Restriction map for
pFN21ASDA0924
(Linear) (Six-base) MAPSORT of: KIAA0924.seq Check: 475 from: 1 to: 1818
KIAA0924.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1512 1655 1818
Size: 1512 143 163
Fragments arranged by size:
1512 163 143
Acc65I G'GTAC_C
Cuts at: 0 773 1818
Size: 773 1045
AclI AA'CG_TT
Cuts at: 0 794 1818
Size: 794 1024
AflII C'TTAA_G
Cuts at: 0 439 1450 1818
Size: 439 1011 368
Fragments arranged by size:
1011 439 368
AleI CACnn'nnGTG
Cuts at: 0 145 1818
Size: 145 1673
AlwNI CAG_nnn'CTG
Cuts at: 0 30 262 1818
Size: 30 232 1556
Fragments arranged by size:
1556 232 30
ApaLI G'TGCA_C
Cuts at: 0 1120 1818
Size: 1120 698
ApoI r'AATT_y
Cuts at: 0 294 982 995 1009 1818
Size: 294 688 13 14 809
Fragments arranged by size:
809 688 294 14 13
BanI G'GyrC_C
Cuts at: 0 115 773 1781 1818
Size: 115 658 1008 37
Fragments arranged by size:
1008 658 115 37
BanII G_rGCy'C
Cuts at: 0 689 1818
Size: 689 1129
BbsI GAAGACnn'nnnn_
Cuts at: 0 290 1818
Size: 290 1528
Bme1580I G_kGCm'C
Cuts at: 0 751 1124 1229 1818
Size: 751 373 105 589
Fragments arranged by size:
751 589 373 105
BmgBI CAC'GTC
Cuts at: 0 267 1818
Size: 267 1551
BmrI ACTGGGnnnn_n'
Cuts at: 0 1537 1818
Size: 1537 281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1160 1328 1679 1703 1818
Size: 799 361 168 351 24 115
Fragments arranged by size:
799 361 351 168 115 24
Bpu10I CC'TnA_GC
Cuts at: 0 659 1818
Size: 659 1159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1818
Size: 1099 719
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 197 227 1818
Size: 197 30 1591
Fragments arranged by size:
1591 197 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 469 1818
Size: 325 144 1349
Fragments arranged by size:
1349 325 144
BseYI C'CCAG_C
Cuts at: 0 1533 1818
Size: 1533 285
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1633 1818
Size: 1633 185
BsiHKAI G_wGCw'C
Cuts at: 0 1124 1818
Size: 1124 694
BsmI GAATG_Cn'
Cuts at: 0 1134 1214 1818
Size: 1134 80 604
Fragments arranged by size:
1134 604 80
Bsp1286I G_dGCh'C
Cuts at: 0 689 751 1124 1229 1818
Size: 689 62 373 105 589
Fragments arranged by size:
689 589 373 105 62
BspHI T'CATG_A
Cuts at: 0 942 1818
Size: 942 876
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1512 1655 1818
Size: 1512 143 163
Fragments arranged by size:
1512 163 143
BsrDI GCAATG_nn'
Cuts at: 0 504 1818
Size: 504 1314
BsrGI T'GTAC_A
Cuts at: 0 149 1818
Size: 149 1669
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1128 1818
Size: 1128 690
BstEII G'GTnAC_C
Cuts at: 0 1479 1818
Size: 1479 339
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1252 1818
Size: 1252 566
BstYI r'GATC_y
Cuts at: 0 1514 1818
Size: 1514 304
BtgI C'CryG_G
Cuts at: 0 963 1019 1818
Size: 963 56 799
Fragments arranged by size:
963 799 56
BtsI GCAGTG_nn'
Cuts at: 0 263 456 1818
Size: 263 193 1362
Fragments arranged by size:
1362 263 193
DraI TTT'AAA
Cuts at: 0 1745 1818
Size: 1745 73
EaeI y'GGCC_r
Cuts at: 0 1422 1818
Size: 1422 396
EarI CTCTTCn'nnn_
Cuts at: 0 311 417 455 461 473 637 713 1363
Size: 311 106 38 6 12 164 76 650
Cuts at: 1363 1616 1818
Size: 253 202
Fragments arranged by size:
650 311 253 202 164 106 76 38
12 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1160 1328 1679 1703 1818
Size: 799 361 168 351 24 115
Fragments arranged by size:
799 361 351 168 115 24
EcoRI G'AATT_C
Cuts at: 0 294 1818
Size: 294 1524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1296 1818
Size: 1296 522
HindIII A'AGCT_T
Cuts at: 0 1344 1818
Size: 1344 474
KpnI G_GTAC'C
Cuts at: 0 777 1818
Size: 777 1041
MscI TGG'CCA
Cuts at: 0 1424 1818
Size: 1424 394
MslI CAynn'nnrTG
Cuts at: 0 145 196 215 968 1071 1818
Size: 145 51 19 753 103 747
Fragments arranged by size:
753 747 145 103 51 19
MspA1I CmG'CkG
Cuts at: 0 1428 1818
Size: 1428 390
NcoI C'CATG_G
Cuts at: 0 963 1019 1818
Size: 963 56 799
Fragments arranged by size:
963 799 56
NspI r_CATG'y
Cuts at: 0 55 274 813 1065 1076 1449 1818
Size: 55 219 539 252 11 373 369
Fragments arranged by size:
539 373 369 252 219 55 11
PasI CC'CwG_GG
Cuts at: 0 752 1818
Size: 752 1066
PfoI T'CCnGG_A
Cuts at: 0 845 1818
Size: 845 973
ScaI AGT'ACT
Cuts at: 0 1279 1818
Size: 1279 539
SmlI C'TyrA_G
Cuts at: 0 439 1114 1450 1818
Size: 439 675 336 368
Fragments arranged by size:
675 439 368 336
SphI G_CATG'C
Cuts at: 0 274 813 1818
Size: 274 539 1005
Fragments arranged by size:
1005 539 274
StyI C'CwwG_G
Cuts at: 0 963 1019 1818
Size: 963 56 799
Fragments arranged by size:
963 799 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1673 1688 1818
Size: 1673 15 130
Fragments arranged by size:
1673 130 15
TatI w'GTAC_w
Cuts at: 0 149 1184 1277 1818
Size: 149 1035 93 541
Fragments arranged by size:
1035 541 149 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1493 1525 1530 1562 1818
Size: 1493 32 5 32 256
Fragments arranged by size:
1493 256 32 32 5
XmnI GAAnn'nnTTC
Cuts at: 0 1009 1178 1818
Size: 1009 169 640
Fragments arranged by size:
1009 640 169
Enzymes that do cut:
AarI Acc65I AclI AflII AleI AlwNI ApaLI ApoI
BanI BanII BbsI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BstAPI BstEII BstXI BstYI
BtgI BtsI DraI EaeI EarI Eco57MI EcoRI FalI
HindIII KpnI MscI MslI MspA1I NcoI NspI PasI
PfoI ScaI SmlI SphI StyI TaqII TatI TstI
XmnI
Enzymes that do not cut:
AatII AccI AcuI AfeI AflIII AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI ZraI