(Circular) (Six-base) MAPSORT of: pFN21ASDA0915.seq Check: 3447 from: 1 to: 7252 pFN21ASDA0915 7252 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4336 4386 4336 Size: 50 7202 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3305 5062 278 Size: 53 83 186 936 1769 1757 2468 Fragments arranged by size: 2468 1769 1757 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4595 1937 Size: 2658 4594 Acc65I G'GTAC_C Cuts at: 4573 4573 Size: 7252 AclI AA'CG_TT Cuts at: 1563 5379 5752 1563 Size: 3816 373 3063 Fragments arranged by size: 3816 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2526 2958 3056 3867 4359 4413 4424 4503 Size: 894 432 98 811 492 54 11 79 Cuts at: 4503 5315 6699 6967 1632 Size: 812 1384 268 1917 Fragments arranged by size: 1917 1384 894 812 811 492 432 268 98 79 54 11 AfeI AGC'GCT Cuts at: 3820 4223 3820 Size: 403 6849 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7055 AflIII A'CryG_T Cuts at: 1203 6151 7128 1203 Size: 4948 977 1327 Fragments arranged by size: 4948 1327 977 AgeI A'CCGG_T Cuts at: 6083 6083 Size: 7252 AhdI GACnn_n'nnGTC Cuts at: 5981 5981 Size: 7252 AleI CACnn'nnGTG Cuts at: 1166 3350 3605 1166 Size: 2184 255 4813 Fragments arranged by size: 4813 2184 255 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2429 2861 3737 1184 Size: 1245 432 876 4699 Fragments arranged by size: 4699 1245 876 432 AlwNI CAG_nnn'CTG Cuts at: 2357 2462 2627 2999 4075 4306 4995 6567 2357 Size: 105 165 372 1076 231 689 1572 3042 Fragments arranged by size: 3042 1572 1076 689 372 231 165 105 ApaLI G'TGCA_C Cuts at: 2194 5309 6465 2194 Size: 3115 1156 2981 Fragments arranged by size: 3115 2981 1156 ApoI r'AATT_y Cuts at: 1493 3929 4561 4787 4939 7048 1493 Size: 2436 632 226 152 2109 1697 Fragments arranged by size: 2436 2109 1697 632 226 152 AseI AT'TA_AT Cuts at: 160 1032 5806 160 Size: 872 4774 1606 Fragments arranged by size: 4774 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7252 AvaI C'yCGr_G Cuts at: 1870 1943 4499 4577 1870 Size: 73 2556 78 4545 Fragments arranged by size: 4545 2556 78 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3062 3095 3062 Size: 33 7219 BamHI G'GATC_C Cuts at: 4120 4582 4949 4120 Size: 462 367 6423 Fragments arranged by size: 6423 462 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2698 3921 3957 4473 4523 Size: 325 217 627 911 1223 36 516 50 Cuts at: 4523 4573 6028 618 Size: 50 1455 1842 Fragments arranged by size: 1842 1455 1223 911 627 516 325 217 50 50 36 BanII G_rGCy'C Cuts at: 1391 2217 2518 2691 4571 1391 Size: 826 301 173 1880 4072 Fragments arranged by size: 4072 1880 826 301 173 BbeI G_GCGC'C Cuts at: 3925 3925 Size: 7252 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3233 928 Size: 971 1334 4947 Fragments arranged by size: 4947 1334 971 BbvCI CC'TCA_GC Cuts at: 2257 2455 3254 3822 2257 Size: 198 799 568 5687 Fragments arranged by size: 5687 799 568 198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 5443 5477 1903 Size: 34 1850 34 1622 34 3678 Fragments arranged by size: 3678 1850 1622 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3241 5144 6354 3241 Size: 1903 1210 4139 Fragments arranged by size: 4139 1903 1210 BclI T'GATC_A Cuts at: 1548 3391 1548 Size: 1843 5409 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3779 5863 136 Size: 107 122 71 631 605 2107 2084 1525 Fragments arranged by size: 2107 2084 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2920 7247 2920 Size: 4327 2925 BlpI GC'TnA_GC Cuts at: 2832 3356 4669 2832 Size: 524 1313 5415 Fragments arranged by size: 5415 1313 524 Bme1580I G_kGCm'C Cuts at: 1790 2198 5313 6469 1790 Size: 408 3115 1156 2573 Fragments arranged by size: 3115 2573 1156 408 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7252 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2026 2110 4983 Size: 416 53 83 399 24 609 84 2873 Cuts at: 4983 5941 442 Size: 958 1753 Fragments arranged by size: 2873 1753 958 609 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7252 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2291 2598 3162 2291 Size: 307 564 6381 Fragments arranged by size: 6381 564 307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2355 2448 2604 2705 2976 3132 3180 4373 Size: 1116 93 156 101 271 156 48 1193 Cuts at: 4373 5912 1239 Size: 1539 2579 Fragments arranged by size: 2579 1539 1193 1116 271 156 156 101 93 48 Bpu10I CC'TnA_GC Cuts at: 2257 2455 2517 3254 3822 2257 Size: 198 62 737 568 5687 Fragments arranged by size: 5687 737 568 198 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2216 2840 5377 6242 6540 2216 Size: 624 2537 865 298 2928 Fragments arranged by size: 2928 2537 865 624 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2373 2549 3572 3913 5915 882 Size: 617 874 176 1023 341 2002 2219 Fragments arranged by size: 2219 2002 1023 874 617 341 176 BsaAI yAC'GTr Cuts at: 493 1204 4549 5043 493 Size: 711 3345 494 2702 Fragments arranged by size: 3345 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2925 7246 2925 Size: 4321 2931 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3302 3922 5059 5441 Size: 53 83 186 936 1769 620 1137 382 Cuts at: 5441 6076 275 Size: 635 1451 Fragments arranged by size: 1769 1451 1137 936 635 620 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4117 5685 6083 6357 6504 6915 4117 Size: 1568 398 274 147 411 4454 Fragments arranged by size: 4454 1568 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2126 2156 3308 3338 1178 Size: 30 165 30 723 30 1152 30 5092 Fragments arranged by size: 5092 1152 723 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2134 2732 2843 2967 2979 3032 3125 3590 Size: 948 598 111 124 12 53 93 465 Cuts at: 3590 4483 1186 Size: 893 3955 Fragments arranged by size: 3955 948 893 598 465 124 111 93 53 12 BseYI C'CCAG_C Cuts at: 2218 4107 4228 4431 6455 6951 2218 Size: 1889 121 203 2024 496 2519 Fragments arranged by size: 2519 2024 1889 496 203 121 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2389 3437 4308 4364 1163 Size: 573 653 1048 871 56 4051 Fragments arranged by size: 4051 1048 871 653 573 56 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4702 5463 6491 1540 Size: 275 186 2701 761 1028 2301 Fragments arranged by size: 2701 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2198 2620 2691 3978 5313 5398 6469 2198 Size: 422 71 1287 1335 85 1071 2981 Fragments arranged by size: 2981 1335 1287 1071 422 85 71 BsmI GAATG_Cn' Cuts at: 3775 4763 4856 3775 Size: 988 93 6171 Fragments arranged by size: 6171 988 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2217 2518 2620 2691 3978 4571 Size: 399 408 19 301 102 71 1287 593 Cuts at: 4571 5313 5398 6469 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1287 1071 742 593 408 399 301 102 85 71 19 BspEI T'CCGG_A Cuts at: 4117 6915 4117 Size: 2798 4454 BspHI T'CATG_A Cuts at: 5141 6871 5141 Size: 1730 5522 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2365 2478 3327 4038 4102 4336 4386 4607 Size: 1521 113 849 711 64 234 50 221 Cuts at: 4607 844 Size: 3489 Fragments arranged by size: 3489 1521 849 711 234 221 113 64 50 BsrBI CCG'CTC Cuts at: 1394 1823 2823 3442 3658 5139 1394 Size: 429 1000 619 216 1481 3507 Fragments arranged by size: 3507 1481 1000 619 429 216 BsrDI GCAATG_nn' Cuts at: 65 1248 5747 5921 65 Size: 1183 4499 174 1396 Fragments arranged by size: 4499 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3630 5896 6083 1700 Size: 110 1820 2266 187 2869 Fragments arranged by size: 2869 2266 1820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7252 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7252 BssSI C'ACGA_G Cuts at: 2874 5312 6324 2874 Size: 2438 1012 3802 Fragments arranged by size: 3802 2438 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7252 BstBI TT'CG_AA Cuts at: 2007 6056 2007 Size: 4049 3203 BstEII G'GTnAC_C Cuts at: 1184 2397 2792 1184 Size: 1213 395 5644 Fragments arranged by size: 5644 1213 395 BstYI r'GATC_y Cuts at: 1972 2920 4120 4582 4949 5334 5351 6803 6889 Size: 948 1200 462 367 385 17 1452 86 Cuts at: 6889 6901 7247 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1200 948 462 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2421 513 Size: 552 1356 5344 Fragments arranged by size: 5344 1356 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4073 487 Size: 683 56 2847 3666 Fragments arranged by size: 3666 2847 683 56 BtsI GCAGTG_nn' Cuts at: 2826 4768 5562 5582 2826 Size: 1942 794 20 4496 Fragments arranged by size: 4496 1942 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7252 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7217 DraI TTT'AAA Cuts at: 4556 4556 Size: 7252 DraIII CAC_nnn'GTG Cuts at: 3207 3523 3207 Size: 316 6936 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6259 809 Size: 507 4943 1802 Fragments arranged by size: 4943 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4699 5588 7217 Size: 54 1425 50 275 18 2869 889 1629 Cuts at: 7217 8 Size: 43 Fragments arranged by size: 2869 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4699 1537 Size: 275 2887 4090 Fragments arranged by size: 4090 2887 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3009 3410 3481 3741 3843 5182 1353 Size: 1656 401 71 260 102 1339 3423 Fragments arranged by size: 3423 1656 1339 401 260 102 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2072 2332 3607 4133 5839 6213 Size: 1064 138 483 260 1275 526 1706 374 Cuts at: 6213 6359 387 Size: 146 1280 Fragments arranged by size: 1706 1280 1275 1064 526 483 374 260 146 138 EcoICRI GAG'CTC Cuts at: 2689 2689 Size: 7252 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2355 2448 2526 2604 2705 2958 2976 Size: 393 723 93 78 78 101 253 18 Cuts at: 2976 3056 3132 3180 3867 4359 4373 4413 4424 Size: 80 76 48 687 492 14 40 11 Cuts at: 4424 4503 5315 5912 6699 6967 1239 Size: 79 812 597 787 268 1524 Fragments arranged by size: 1524 812 787 723 687 597 492 393 268 253 101 93 80 79 78 78 76 48 40 18 14 11 EcoNI CCTnn'n_nnAGG Cuts at: 3209 4100 4518 3209 Size: 891 418 5943 Fragments arranged by size: 5943 891 418 EcoO109I rG'GnC_Cy Cuts at: 2126 2601 2722 2731 2905 3527 3781 3895 4250 Size: 475 121 9 174 622 254 114 355 Cuts at: 4250 4419 2126 Size: 169 4959 Fragments arranged by size: 4959 622 475 355 254 174 169 121 114 9 EcoRI G'AATT_C Cuts at: 3929 4561 4939 3929 Size: 632 378 6242 Fragments arranged by size: 6242 632 378 FspI TGC'GCA Cuts at: 3713 5758 3713 Size: 2045 5207 HaeII r_GCGC'y Cuts at: 1678 2341 3822 3925 4225 6399 1678 Size: 663 1481 103 300 2174 2531 Fragments arranged by size: 2531 2174 1481 663 300 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5893 5925 5967 5999 1373 Size: 32 135 32 4321 32 42 32 2626 Fragments arranged by size: 4321 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4596 4842 669 Size: 988 281 2658 246 3079 Fragments arranged by size: 3079 2658 988 281 246 HindIII A'AGCT_T Cuts at: 748 4197 748 Size: 3449 3803 HpaI GTT'AAC Cuts at: 4842 4842 Size: 7252 KasI G'GCGC_C Cuts at: 3921 3921 Size: 7252 KpnI G_GTAC'C Cuts at: 4577 4577 Size: 7252 MfeI C'AATT_G Cuts at: 4851 4851 Size: 7252 MluI A'CGCG_T Cuts at: 7128 7128 Size: 7252 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4489 6366 6550 1305 Size: 3184 1877 184 2007 Fragments arranged by size: 3184 2007 1877 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5430 Fragments arranged by size: 5430 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3350 3605 5210 5569 5728 518 Size: 648 2184 255 1605 359 159 2042 Fragments arranged by size: 2184 2042 1605 648 359 255 159 MspA1I CmG'CkG Cuts at: 1606 2222 2752 3237 3667 4063 4435 4668 5345 Size: 616 530 485 430 396 372 233 677 Cuts at: 5345 6493 6738 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 616 530 485 430 396 372 245 233 NaeI GCC'GGC Cuts at: 3632 3632 Size: 7252 NarI GG'CG_CC Cuts at: 3922 3922 Size: 7252 NcoI C'CATG_G Cuts at: 513 1065 2421 513 Size: 552 1356 5344 Fragments arranged by size: 5344 1356 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7252 NgoMIV G'CCGG_C Cuts at: 3630 3630 Size: 7252 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7252 NotI GC'GGCC_GC Cuts at: 4699 4699 Size: 7252 NspI r_CATG'y Cuts at: 1132 1229 3277 3515 3718 4219 4610 6155 1132 Size: 97 2048 238 203 501 391 1545 2229 Fragments arranged by size: 2229 2048 1545 501 391 238 203 97 PasI CC'CwG_GG Cuts at: 1348 2123 2727 3784 1348 Size: 775 604 1057 4816 Fragments arranged by size: 4816 1057 775 604 PciI A'CATG_T Cuts at: 6151 6151 Size: 7252 PflMI CCAn_nnn'nTGG Cuts at: 1108 2208 2627 3523 1108 Size: 1100 419 896 4837 Fragments arranged by size: 4837 1100 896 419 PfoI T'CCnGG_A Cuts at: 3369 3369 Size: 7252 PmeI GTTT'AAAC Cuts at: 4556 4556 Size: 7252 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5321 5353 6834 6866 5321 Size: 32 1481 32 5707 Fragments arranged by size: 5707 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2601 2722 2731 2905 2601 Size: 121 9 174 6948 Fragments arranged by size: 6948 174 121 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7252 PsiI TTA'TAA Cuts at: 4822 4822 Size: 7252 PstI C_TGCA'G Cuts at: 830 2047 3066 3639 3726 4604 830 Size: 1217 1019 573 87 878 3478 Fragments arranged by size: 3478 1217 1019 878 573 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7252 PvuII CAG'CTG Cuts at: 2222 4435 2222 Size: 2213 5039 SacI G_AGCT'C Cuts at: 2691 2691 Size: 7252 SalI G'TCGA_C Cuts at: 1936 4594 1936 Size: 2658 4594 SanDI GG'GwC_CC Cuts at: 2601 2731 2905 2601 Size: 130 174 6948 Fragments arranged by size: 6948 174 130 SapI GCTCTTCn'nnn_ Cuts at: 3481 3843 3481 Size: 362 6890 SbfI CC_TGCA'GG Cuts at: 3066 4604 3066 Size: 1538 5714 ScaI AGT'ACT Cuts at: 5500 7212 5500 Size: 1712 5540 SfcI C'TryA_G Cuts at: 826 1045 2043 3062 3583 3635 3722 4600 5735 Size: 219 998 1019 521 52 87 878 1135 Cuts at: 5735 6416 6607 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1019 998 878 681 521 219 191 87 52 SfoI GGC'GCC Cuts at: 3923 3923 Size: 7252 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7252 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7252 SmaI CCC'GGG Cuts at: 1872 4579 1872 Size: 2707 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2231 2855 4499 5356 6257 6519 Size: 197 926 288 624 1644 857 901 262 Cuts at: 6519 820 Size: 1553 Fragments arranged by size: 1644 1553 926 901 857 624 288 262 197 SnaBI TAC'GTA Cuts at: 493 4549 5043 493 Size: 4056 494 2702 Fragments arranged by size: 4056 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7252 SphI G_CATG'C Cuts at: 1132 3277 3515 3718 4610 1132 Size: 2145 238 203 892 3774 Fragments arranged by size: 3774 2145 892 238 203 SspI AAT'ATT Cuts at: 5 52 5176 5 Size: 47 5124 2081 Fragments arranged by size: 5124 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7252 StyI C'CwwG_G Cuts at: 513 1065 2421 4691 513 Size: 552 1356 2270 3074 Fragments arranged by size: 3074 2270 1356 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2398 2686 2780 3116 3184 3356 3776 Size: 1255 818 288 94 336 68 172 420 Cuts at: 3776 3881 4196 5279 5296 5449 5634 6079 325 Size: 105 315 1083 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1083 818 445 420 336 315 288 185 172 153 105 94 68 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5498 7210 96 Size: 274 80 33 51 1443 3521 1712 138 Fragments arranged by size: 3521 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2126 2158 2308 2340 3159 3191 7212 7244 2126 Size: 32 150 32 819 32 4021 32 2134 Fragments arranged by size: 4021 2134 819 150 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4347 1316 Size: 3031 4221 XbaI T'CTAG_A Cuts at: 2264 4588 2264 Size: 2324 4928 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3241 3241 Size: 7252 XhoI C'TCGA_G Cuts at: 1943 4499 1943 Size: 2556 4696 XmaI C'CCGG_G Cuts at: 1870 4577 1870 Size: 2707 4545 XmnI GAAnn'nnTTC Cuts at: 5381 5381 Size: 7252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3303 5060 276 Size: 53 83 186 936 1769 1757 2468 Fragments arranged by size: 2468 1769 1757 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI