Restriction map for
pFN21ASDA0915
(Linear) (Six-base) MAPSORT of: KIAA0915.seq Check: 9670 from: 1 to: 2523
KIAA0915.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2313 2363 2523
Size: 2313 50 160
Fragments arranged by size:
2313 160 50
AatII G_ACGT'C
Cuts at: 0 1282 2523
Size: 1282 1241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 935 1033 1844 2336 2390 2401 2480
Size: 503 432 98 811 492 54 11 79
Cuts at: 2480 2523
Size: 43
Fragments arranged by size:
811 503 492 432 98 79 54 43
11
AfeI AGC'GCT
Cuts at: 0 1797 2200 2523
Size: 1797 403 323
Fragments arranged by size:
1797 403 323
AleI CACnn'nnGTG
Cuts at: 0 1327 1582 2523
Size: 1327 255 941
Fragments arranged by size:
1327 941 255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 838 1714 2523
Size: 406 432 876 809
Fragments arranged by size:
876 809 432 406
AlwNI CAG_nnn'CTG
Cuts at: 0 334 439 604 976 2052 2283 2523
Size: 334 105 165 372 1076 231 240
Fragments arranged by size:
1076 372 334 240 231 165 105
ApaLI G'TGCA_C
Cuts at: 0 171 2523
Size: 171 2352
ApoI r'AATT_y
Cuts at: 0 1906 2523
Size: 1906 617
AvaI C'yCGr_G
Cuts at: 0 2476 2523
Size: 2476 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1039 1072 2523
Size: 1039 33 1451
Fragments arranged by size:
1451 1039 33
BamHI G'GATC_C
Cuts at: 0 2097 2523
Size: 2097 426
BanI G'GyrC_C
Cuts at: 0 675 1898 1934 2450 2500 2523
Size: 675 1223 36 516 50 23
Fragments arranged by size:
1223 675 516 50 36 23
BanII G_rGCy'C
Cuts at: 0 194 495 668 2523
Size: 194 301 173 1855
Fragments arranged by size:
1855 301 194 173
BbeI G_GCGC'C
Cuts at: 0 1902 2523
Size: 1902 621
BbsI GAAGACnn'nnnn_
Cuts at: 0 1210 2523
Size: 1210 1313
BbvCI CC'TCA_GC
Cuts at: 0 234 432 1231 1799 2523
Size: 234 198 799 568 724
Fragments arranged by size:
799 724 568 234 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1764 1798 2523
Size: 1764 34 725
Fragments arranged by size:
1764 725 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1218 2523
Size: 1218 1305
BclI T'GATC_A
Cuts at: 0 1368 2523
Size: 1368 1155
BglI GCCn_nnn'nGGC
Cuts at: 0 1756 2523
Size: 1756 767
BglII A'GATC_T
Cuts at: 0 897 2523
Size: 897 1626
BlpI GC'TnA_GC
Cuts at: 0 809 1333 2523
Size: 809 524 1190
Fragments arranged by size:
1190 809 524
Bme1580I G_kGCm'C
Cuts at: 0 175 2523
Size: 175 2348
BmrI ACTGGGnnnn_n'
Cuts at: 0 3 87 2523
Size: 3 84 2436
Fragments arranged by size:
2436 84 3
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 268 575 1139 2523
Size: 268 307 564 1384
Fragments arranged by size:
1384 564 307 268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 425 581 682 953 1109 1157 2350
Size: 332 93 156 101 271 156 48 1193
Cuts at: 2350 2523
Size: 173
Fragments arranged by size:
1193 332 271 173 156 156 101 93
48
Bpu10I CC'TnA_GC
Cuts at: 0 234 432 494 1231 1799 2523
Size: 234 198 62 737 568 724
Fragments arranged by size:
737 724 568 234 198 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 817 2523
Size: 193 624 1706
Fragments arranged by size:
1706 624 193
BsaI GGTCTCn'nnnn_
Cuts at: 0 350 526 1549 1890 2523
Size: 350 176 1023 341 633
Fragments arranged by size:
1023 633 350 341 176
BsaBI GATnn'nnATC
Cuts at: 0 902 2523
Size: 902 1621
BsaHI Gr'CG_yC
Cuts at: 0 1279 1899 2523
Size: 1279 620 624
Fragments arranged by size:
1279 624 620
BsaWI w'CCGG_w
Cuts at: 0 2094 2523
Size: 2094 429
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 1285 1315 2523
Size: 103 30 1152 30 1208
Fragments arranged by size:
1208 1152 103 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 111 709 820 944 956 1009 1102 1567
Size: 111 598 111 124 12 53 93 465
Cuts at: 1567 2460 2523
Size: 893 63
Fragments arranged by size:
893 598 465 124 111 111 93 63
53 12
BseYI C'CCAG_C
Cuts at: 0 195 2084 2205 2408 2523
Size: 195 1889 121 203 115
Fragments arranged by size:
1889 203 195 121 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 1414 2285 2341 2523
Size: 366 1048 871 56 182
Fragments arranged by size:
1048 871 366 182 56
BsiHKAI G_wGCw'C
Cuts at: 0 175 597 668 1955 2523
Size: 175 422 71 1287 568
Fragments arranged by size:
1287 568 422 175 71
BsmI GAATG_Cn'
Cuts at: 0 1752 2523
Size: 1752 771
Bsp1286I G_dGCh'C
Cuts at: 0 175 194 495 597 668 1955 2523
Size: 175 19 301 102 71 1287 568
Fragments arranged by size:
1287 568 301 175 102 71 19
BspEI T'CCGG_A
Cuts at: 0 2094 2523
Size: 2094 429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 342 455 1304 2015 2079 2313 2363 2523
Size: 342 113 849 711 64 234 50 160
Fragments arranged by size:
849 711 342 234 160 113 64 50
BsrBI CCG'CTC
Cuts at: 0 800 1419 1635 2523
Size: 800 619 216 888
Fragments arranged by size:
888 800 619 216
BsrFI r'CCGG_y
Cuts at: 0 1607 2523
Size: 1607 916
BssSI C'ACGA_G
Cuts at: 0 851 2523
Size: 851 1672
BstEII G'GTnAC_C
Cuts at: 0 374 769 2523
Size: 374 395 1754
Fragments arranged by size:
1754 395 374
BstYI r'GATC_y
Cuts at: 0 897 2097 2523
Size: 897 1200 426
Fragments arranged by size:
1200 897 426
BtgI C'CryG_G
Cuts at: 0 398 2523
Size: 398 2125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2050 2523
Size: 2050 473
BtsI GCAGTG_nn'
Cuts at: 0 803 2523
Size: 803 1720
DraIII CAC_nnn'GTG
Cuts at: 0 1184 1500 2523
Size: 1184 316 1023
Fragments arranged by size:
1184 1023 316
EarI CTCTTCn'nnn_
Cuts at: 0 986 1387 1458 1718 1820 2523
Size: 986 401 71 260 102 703
Fragments arranged by size:
986 703 401 260 102 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 49 309 1584 2110 2523
Size: 49 260 1275 526 413
Fragments arranged by size:
1275 526 413 260 49
EcoICRI GAG'CTC
Cuts at: 0 666 2523
Size: 666 1857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 425 503 581 682 935 953 1033
Size: 332 93 78 78 101 253 18 80
Cuts at: 1033 1109 1157 1844 2336 2350 2390 2401 2480
Size: 76 48 687 492 14 40 11 79
Cuts at: 2480 2523
Size: 43
Fragments arranged by size:
687 492 332 253 101 93 80 79
78 78 76 48 43 40 18 14
11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1186 2077 2495 2523
Size: 1186 891 418 28
Fragments arranged by size:
1186 891 418 28
EcoO109I rG'GnC_Cy
Cuts at: 0 103 578 699 708 882 1504 1758 1872
Size: 103 475 121 9 174 622 254 114
Cuts at: 1872 2227 2396 2523
Size: 355 169 127
Fragments arranged by size:
622 475 355 254 174 169 127 121
114 103 9
EcoRI G'AATT_C
Cuts at: 0 1906 2523
Size: 1906 617
FspI TGC'GCA
Cuts at: 0 1690 2523
Size: 1690 833
HaeII r_GCGC'y
Cuts at: 0 318 1799 1902 2202 2523
Size: 318 1481 103 300 321
Fragments arranged by size:
1481 321 318 300 103
HindIII A'AGCT_T
Cuts at: 0 2174 2523
Size: 2174 349
KasI G'GCGC_C
Cuts at: 0 1898 2523
Size: 1898 625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2466 2523
Size: 2466 57
MslI CAynn'nnrTG
Cuts at: 0 1327 1582 2523
Size: 1327 255 941
Fragments arranged by size:
1327 941 255
MspA1I CmG'CkG
Cuts at: 0 199 729 1214 1644 2040 2412 2523
Size: 199 530 485 430 396 372 111
Fragments arranged by size:
530 485 430 396 372 199 111
NaeI GCC'GGC
Cuts at: 0 1609 2523
Size: 1609 914
NarI GG'CG_CC
Cuts at: 0 1899 2523
Size: 1899 624
NcoI C'CATG_G
Cuts at: 0 398 2523
Size: 398 2125
NgoMIV G'CCGG_C
Cuts at: 0 1607 2523
Size: 1607 916
NspI r_CATG'y
Cuts at: 0 1254 1492 1695 2196 2523
Size: 1254 238 203 501 327
Fragments arranged by size:
1254 501 327 238 203
PasI CC'CwG_GG
Cuts at: 0 100 704 1761 2523
Size: 100 604 1057 762
Fragments arranged by size:
1057 762 604 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 185 604 1500 2523
Size: 185 419 896 1023
Fragments arranged by size:
1023 896 419 185
PfoI T'CCnGG_A
Cuts at: 0 1346 2523
Size: 1346 1177
PpuMI rG'GwC_Cy
Cuts at: 0 578 699 708 882 2523
Size: 578 121 9 174 1641
Fragments arranged by size:
1641 578 174 121 9
PstI C_TGCA'G
Cuts at: 0 24 1043 1616 1703 2523
Size: 24 1019 573 87 820
Fragments arranged by size:
1019 820 573 87 24
PvuII CAG'CTG
Cuts at: 0 199 2412 2523
Size: 199 2213 111
Fragments arranged by size:
2213 199 111
SacI G_AGCT'C
Cuts at: 0 668 2523
Size: 668 1855
SanDI GG'GwC_CC
Cuts at: 0 578 708 882 2523
Size: 578 130 174 1641
Fragments arranged by size:
1641 578 174 130
SapI GCTCTTCn'nnn_
Cuts at: 0 1458 1820 2523
Size: 1458 362 703
Fragments arranged by size:
1458 703 362
SbfI CC_TGCA'GG
Cuts at: 0 1043 2523
Size: 1043 1480
SfcI C'TryA_G
Cuts at: 0 20 1039 1560 1612 1699 2523
Size: 20 1019 521 52 87 824
Fragments arranged by size:
1019 824 521 87 52 20
SfoI GGC'GCC
Cuts at: 0 1900 2523
Size: 1900 623
SmlI C'TyrA_G
Cuts at: 0 208 832 2476 2523
Size: 208 624 1644 47
Fragments arranged by size:
1644 624 208 47
SphI G_CATG'C
Cuts at: 0 1254 1492 1695 2523
Size: 1254 238 203 828
Fragments arranged by size:
1254 828 238 203
StyI C'CwwG_G
Cuts at: 0 398 2523
Size: 398 2125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 375 663 757 1093 1161 1333 1753 1858
Size: 375 288 94 336 68 172 420 105
Cuts at: 1858 2173 2523
Size: 315 350
Fragments arranged by size:
420 375 350 336 315 288 172 105
94 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 103 135 285 317 1136 1168 2523
Size: 103 32 150 32 819 32 1355
Fragments arranged by size:
1355 819 150 103 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2324 2523
Size: 2324 199
XbaI T'CTAG_A
Cuts at: 0 241 2523
Size: 241 2282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1218 2523
Size: 1218 1305
XhoI C'TCGA_G
Cuts at: 0 2476 2523
Size: 2476 47
ZraI GAC'GTC
Cuts at: 0 1280 2523
Size: 1280 1243
Enzymes that do cut:
AarI AatII AcuI AfeI AleI AlfI AlwNI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrFI BssSI BstEII BstYI BtgI
BtgZI BtsI DraIII EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII HindIII KasI MmeI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PpuMI PstI PvuII SacI SanDI SapI SbfI
SfcI SfoI SmlI SphI StyI TaqII TstI Tth111I
XbaI XcmI XhoI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BfrBI BmgBI BmtI
BsaAI BsiEI BsiWI BsmBI BspHI BsrDI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EaeI EagI EcoRV FalI FseI FspAI Hin4I
HincII HpaI KpnI MfeI MluI MscI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TatI XmaI XmnI