(Circular) (Six-base) MAPSORT of: pFN21ASDA0907.seq Check: 2527 from: 1 to: 6421 pFN21ASDA0907 6421 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4231 278 Size: 53 83 186 936 2695 2468 Fragments arranged by size: 2695 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3714 3764 1937 Size: 1777 50 4594 Fragments arranged by size: 4594 1777 50 Acc65I G'GTAC_C Cuts at: 3742 3742 Size: 6421 AclI AA'CG_TT Cuts at: 1563 4548 4921 1563 Size: 2985 373 3063 Fragments arranged by size: 3063 2985 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2163 2264 2358 4484 5868 6136 1632 Size: 531 101 94 2126 1384 268 1917 Fragments arranged by size: 2126 1917 1384 531 268 101 94 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6224 AflIII A'CryG_T Cuts at: 1203 2366 5320 6297 1203 Size: 1163 2954 977 1327 Fragments arranged by size: 2954 1327 1163 977 AgeI A'CCGG_T Cuts at: 5252 5252 Size: 6421 AhdI GACnn_n'nnGTC Cuts at: 5150 5150 Size: 6421 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6421 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6421 AlwNI CAG_nnn'CTG Cuts at: 2630 2876 3608 4164 5736 2630 Size: 246 732 556 1572 3315 Fragments arranged by size: 3315 1572 732 556 246 ApaLI G'TGCA_C Cuts at: 4478 5634 4478 Size: 1156 5265 ApoI r'AATT_y Cuts at: 1493 3730 3956 4108 6217 1493 Size: 2237 226 152 2109 1697 Fragments arranged by size: 2237 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2220 2349 3012 4975 160 Size: 872 1188 129 663 1963 1606 Fragments arranged by size: 1963 1606 1188 872 663 129 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 AvaI C'yCGr_G Cuts at: 1870 1943 2385 2439 3746 1870 Size: 73 442 54 1307 4545 Fragments arranged by size: 4545 1307 442 73 54 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3006 3039 3006 Size: 33 6388 BamHI G'GATC_C Cuts at: 3751 4118 3751 Size: 367 6054 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2061 2162 2825 3742 5197 Size: 325 217 627 274 101 663 917 1455 Cuts at: 5197 618 Size: 1842 Fragments arranged by size: 1842 1455 917 663 627 325 274 217 101 BanII G_rGCy'C Cuts at: 1391 3740 1391 Size: 2349 4072 BbeI G_GCGC'C Cuts at: 2065 2065 Size: 6421 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2827 928 Size: 971 928 4522 Fragments arranged by size: 4522 971 928 BbvCI CC'TCA_GC Cuts at: 3132 3132 Size: 6421 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4612 4646 1903 Size: 34 2675 34 3678 Fragments arranged by size: 3678 2675 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3449 4313 5523 3449 Size: 864 1210 4347 Fragments arranged by size: 4347 1210 864 BclI T'GATC_A Cuts at: 1548 1548 Size: 6421 BfrBI ATG'CAT Cuts at: 2707 2707 Size: 6421 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5032 136 Size: 107 122 71 631 605 3360 1525 Fragments arranged by size: 3360 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6416 6416 Size: 6421 BlpI GC'TnA_GC Cuts at: 3838 3838 Size: 6421 Bme1580I G_kGCm'C Cuts at: 1790 2165 4482 5638 1790 Size: 375 2317 1156 2573 Fragments arranged by size: 2573 2317 1156 375 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6421 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4152 5110 442 Size: 416 53 83 399 24 2735 958 1753 Fragments arranged by size: 2735 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3279 1056 Size: 2223 4198 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2070 2073 2652 3219 3243 3255 3487 5081 Size: 831 3 579 567 24 12 232 1594 Cuts at: 5081 1239 Size: 2579 Fragments arranged by size: 2579 1594 831 579 567 232 24 12 3 Bpu10I CC'TnA_GC Cuts at: 3132 3132 Size: 6421 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2382 4546 5411 5709 2382 Size: 2164 865 298 3094 Fragments arranged by size: 3094 2164 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5084 882 Size: 617 3585 2219 Fragments arranged by size: 3585 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3718 4212 493 Size: 711 2514 494 2702 Fragments arranged by size: 2702 2514 711 494 BsaBI GATnn'nnATC Cuts at: 6415 6415 Size: 6421 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2062 4228 4610 5245 Size: 53 83 186 936 529 2166 382 635 Cuts at: 5245 275 Size: 1451 Fragments arranged by size: 2166 1451 936 635 529 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2550 4854 5252 5526 5673 6084 2550 Size: 2304 398 274 147 411 2887 Fragments arranged by size: 2887 2304 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2158 2188 3652 3682 1178 Size: 30 165 30 755 30 1464 30 3917 Fragments arranged by size: 3917 1464 755 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3060 3120 3434 1186 Size: 1874 60 314 4173 Fragments arranged by size: 4173 1874 314 60 BseYI C'CCAG_C Cuts at: 3351 3357 3363 3369 3683 5624 6120 3351 Size: 6 6 6 314 1941 496 3652 Fragments arranged by size: 3652 1941 496 314 6 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3284 3546 1163 Size: 573 1548 262 4038 Fragments arranged by size: 4038 1548 573 262 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3871 4632 5660 1540 Size: 275 186 1870 761 1028 2301 Fragments arranged by size: 2301 1870 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4482 4567 5638 4482 Size: 85 1071 5265 Fragments arranged by size: 5265 1071 85 BsmI GAATG_Cn' Cuts at: 2269 3932 4025 2269 Size: 1663 93 4665 Fragments arranged by size: 4665 1663 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3400 3400 Size: 6421 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 3740 4482 4567 5638 1391 Size: 399 375 1575 742 85 1071 2174 Fragments arranged by size: 2174 1575 1071 742 399 375 85 BspEI T'CCGG_A Cuts at: 6084 6084 Size: 6421 BspHI T'CATG_A Cuts at: 2451 4310 6040 2451 Size: 1859 1730 2832 Fragments arranged by size: 2832 1859 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3776 844 Size: 2932 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4308 1394 Size: 429 2485 3507 Fragments arranged by size: 3507 2485 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4916 5090 65 Size: 1183 3668 174 1396 Fragments arranged by size: 3668 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3208 5065 5252 1700 Size: 110 1398 1857 187 2869 Fragments arranged by size: 2869 1857 1398 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6421 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6421 BssSI C'ACGA_G Cuts at: 4481 5493 4481 Size: 1012 5409 BstAPI GCAn_nnn'nTGC Cuts at: 794 3583 794 Size: 2789 3632 BstBI TT'CG_AA Cuts at: 2007 5225 2007 Size: 3218 3203 BstEII G'GTnAC_C Cuts at: 1184 2134 3110 1184 Size: 950 976 4495 Fragments arranged by size: 4495 976 950 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2578 2233 Size: 345 6076 BstYI r'GATC_y Cuts at: 1972 3751 4118 4503 4520 5972 6058 6070 6416 Size: 1779 367 385 17 1452 86 12 346 Cuts at: 6416 1972 Size: 1977 Fragments arranged by size: 1977 1779 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3550 3550 Size: 6421 BtgI C'CryG_G Cuts at: 513 1065 2028 3664 513 Size: 552 963 1636 3270 Fragments arranged by size: 3270 1636 963 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2638 487 Size: 683 56 1412 4270 Fragments arranged by size: 4270 1412 683 56 BtsI GCAGTG_nn' Cuts at: 2149 3937 4731 4751 2149 Size: 1788 794 20 3819 Fragments arranged by size: 3819 1788 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6421 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6386 DraI TTT'AAA Cuts at: 3725 3725 Size: 6421 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5428 809 Size: 507 4112 1802 Fragments arranged by size: 4112 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2878 3868 4757 Size: 54 1425 50 275 18 1048 990 889 Cuts at: 4757 6386 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1048 990 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3868 1537 Size: 275 2056 4090 Fragments arranged by size: 4090 2056 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 3398 4351 1353 Size: 755 1290 953 3423 Fragments arranged by size: 3423 1290 953 755 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5008 5382 5528 387 Size: 1064 138 3419 374 146 1280 Fragments arranged by size: 3419 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2070 2073 2163 2264 2358 2652 3219 Size: 393 438 3 90 101 94 294 567 Cuts at: 3219 3243 3255 3487 4484 5081 5868 6136 1239 Size: 24 12 232 997 597 787 268 1524 Fragments arranged by size: 1524 997 787 597 567 438 393 294 268 232 101 94 90 24 12 3 EcoNI CCTnn'n_nnAGG Cuts at: 2292 3030 2292 Size: 738 5683 EcoO109I rG'GnC_Cy Cuts at: 3648 3648 Size: 6421 EcoRI G'AATT_C Cuts at: 3730 4108 3730 Size: 378 6043 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2625 2707 2625 Size: 82 6339 FspI TGC'GCA Cuts at: 4927 4927 Size: 6421 HaeII r_GCGC'y Cuts at: 1678 2065 5568 1678 Size: 387 3503 2531 Fragments arranged by size: 3503 2531 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2858 2890 3286 3318 5062 Size: 32 135 32 1286 32 396 32 1744 Cuts at: 5062 5094 5136 5168 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1744 1286 396 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3765 4011 669 Size: 988 281 1827 246 3079 Fragments arranged by size: 3079 1827 988 281 246 HindIII A'AGCT_T Cuts at: 748 2276 2886 2951 748 Size: 1528 610 65 4218 Fragments arranged by size: 4218 1528 610 65 HpaI GTT'AAC Cuts at: 4011 4011 Size: 6421 KasI G'GCGC_C Cuts at: 2061 2061 Size: 6421 KpnI G_GTAC'C Cuts at: 3746 3746 Size: 6421 MfeI C'AATT_G Cuts at: 4020 4020 Size: 6421 MluI A'CGCG_T Cuts at: 6297 6297 Size: 6421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2630 5535 5719 1305 Size: 1325 2905 184 2007 Fragments arranged by size: 2905 2007 1325 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4599 Fragments arranged by size: 4599 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 3476 4379 4738 4897 518 Size: 648 1065 1245 903 359 159 2042 Fragments arranged by size: 2042 1245 1065 903 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2117 2171 2672 3182 3837 4514 5662 Size: 424 87 54 501 510 655 677 1148 Cuts at: 5662 5907 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 655 510 501 424 245 87 54 NaeI GCC'GGC Cuts at: 3210 3210 Size: 6421 NarI GG'CG_CC Cuts at: 2062 2062 Size: 6421 NcoI C'CATG_G Cuts at: 513 1065 3664 513 Size: 552 2599 3270 Fragments arranged by size: 3270 2599 552 NdeI CA'TA_TG Cuts at: 387 3477 387 Size: 3090 3331 NgoMIV G'CCGG_C Cuts at: 3208 3208 Size: 6421 NheI G'CTAG_C Cuts at: 1052 3275 1052 Size: 2223 4198 NotI GC'GGCC_GC Cuts at: 3868 3868 Size: 6421 NsiI A_TGCA'T Cuts at: 2709 2709 Size: 6421 NspI r_CATG'y Cuts at: 1132 1229 2370 2747 3779 5324 1132 Size: 97 1141 377 1032 1545 2229 Fragments arranged by size: 2229 1545 1141 1032 377 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6421 PciI A'CATG_T Cuts at: 2366 5320 2366 Size: 2954 3467 PflMI CCAn_nnn'nTGG Cuts at: 1108 2876 3095 3608 1108 Size: 1768 219 513 3921 Fragments arranged by size: 3921 1768 513 219 PfoI T'CCnGG_A Cuts at: 2047 2047 Size: 6421 PmeI GTTT'AAAC Cuts at: 3725 3725 Size: 6421 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4490 4522 6003 6035 4490 Size: 32 1481 32 4876 Fragments arranged by size: 4876 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3648 3648 Size: 6421 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6421 PsiI TTA'TAA Cuts at: 3991 3991 Size: 6421 PspXI vC'TCGA_Gb Cuts at: 2385 2439 2385 Size: 54 6367 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2733 2765 2733 Size: 32 6389 PstI C_TGCA'G Cuts at: 830 2431 3773 830 Size: 1601 1342 3478 Fragments arranged by size: 3478 1601 1342 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6421 PvuII CAG'CTG Cuts at: 2171 2672 3182 2171 Size: 501 510 5410 Fragments arranged by size: 5410 510 501 SacII CC_GC'GG Cuts at: 2031 2031 Size: 6421 SalI G'TCGA_C Cuts at: 1936 3763 1936 Size: 1827 4594 SbfI CC_TGCA'GG Cuts at: 3773 3773 Size: 6421 ScaI AGT'ACT Cuts at: 4669 6381 4669 Size: 1712 4709 SexAI A'CCwGG_T Cuts at: 2325 2325 Size: 6421 SfcI C'TryA_G Cuts at: 826 1045 2427 3769 4904 5585 5776 826 Size: 219 1382 1342 1135 681 191 1471 Fragments arranged by size: 1471 1382 1342 1135 681 219 191 SfoI GGC'GCC Cuts at: 2063 2063 Size: 6421 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6421 SmaI CCC'GGG Cuts at: 1872 3748 1872 Size: 1876 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2385 2397 2439 4525 5426 5688 Size: 197 926 442 12 42 2086 901 262 Cuts at: 5688 820 Size: 1553 Fragments arranged by size: 2086 1553 926 901 442 262 197 42 12 SnaBI TAC'GTA Cuts at: 493 3718 4212 493 Size: 3225 494 2702 Fragments arranged by size: 3225 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6421 SphI G_CATG'C Cuts at: 1132 3779 1132 Size: 2647 3774 SspI AAT'ATT Cuts at: 5 52 4345 5 Size: 47 4293 2081 Fragments arranged by size: 4293 2081 47 StuI AGG'CCT Cuts at: 1517 2298 2934 1517 Size: 781 636 5004 Fragments arranged by size: 5004 781 636 StyI C'CwwG_G Cuts at: 513 1065 2187 3664 3860 513 Size: 552 1122 1477 196 3074 Fragments arranged by size: 3074 1477 1122 552 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2665 3287 4448 4465 4618 4803 5248 Size: 1255 1085 622 1161 17 153 185 445 Cuts at: 5248 325 Size: 1498 Fragments arranged by size: 1498 1255 1161 1085 622 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4667 6379 96 Size: 274 80 33 51 1443 2690 1712 138 Fragments arranged by size: 2690 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2088 2120 2158 2190 3652 3684 6381 6413 2088 Size: 32 38 32 1462 32 2697 32 2096 Fragments arranged by size: 2697 2096 1462 38 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3288 1316 Size: 1972 4449 XbaI T'CTAG_A Cuts at: 2737 2993 3757 2737 Size: 256 764 5401 Fragments arranged by size: 5401 764 256 XhoI C'TCGA_G Cuts at: 1943 2385 2439 1943 Size: 442 54 5925 Fragments arranged by size: 5925 442 54 XmaI C'CCGG_G Cuts at: 1870 3746 1870 Size: 1876 4545 XmnI GAAnn'nnTTC Cuts at: 4550 4550 Size: 6421 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4229 276 Size: 53 83 186 936 2695 2468 Fragments arranged by size: 2695 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI PacI PmlI PspOMI RsrII SacI SanDI SapI SfiI SrfI SwaI XcmI