(Circular) (Six-base) MAPSORT of: pFN21ASDA0895.seq Check: 1074 from: 1 to: 6136 pFN21ASDA0895 6136 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3946 278 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3198 3479 1937 Size: 1261 281 4594 Fragments arranged by size: 4594 1261 281 Acc65I G'GTAC_C Cuts at: 3457 3457 Size: 6136 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4199 5583 5851 1632 Size: 2567 1384 268 1917 Fragments arranged by size: 2567 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2729 820 Size: 197 1712 4227 Fragments arranged by size: 4227 1712 197 AflIII A'CryG_T Cuts at: 1203 5035 6012 1203 Size: 3832 977 1327 Fragments arranged by size: 3832 1327 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 4865 4865 Size: 6136 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6136 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2091 2477 1184 Size: 907 386 4843 Fragments arranged by size: 4843 907 386 AlwNI CAG_nnn'CTG Cuts at: 3879 5451 3879 Size: 1572 4564 ApaLI G'TGCA_C Cuts at: 4193 5349 4193 Size: 1156 4980 ApoI r'AATT_y Cuts at: 1493 2573 3210 3445 3671 3823 5932 1493 Size: 1080 637 235 226 152 2109 1697 Fragments arranged by size: 2109 1697 1080 637 235 226 152 AseI AT'TA_AT Cuts at: 160 1032 4690 160 Size: 872 3658 1606 Fragments arranged by size: 3658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 2149 2769 3266 3461 1870 Size: 73 206 620 497 195 4545 Fragments arranged by size: 4545 620 497 206 195 73 BamHI G'GATC_C Cuts at: 3466 3833 3466 Size: 367 5769 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2335 2493 3457 4912 618 Size: 325 217 627 548 158 964 1455 1842 Fragments arranged by size: 1842 1455 964 627 548 325 217 158 BanII G_rGCy'C Cuts at: 1391 3455 1391 Size: 2064 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2238 928 Size: 971 339 4826 Fragments arranged by size: 4826 971 339 BbvCI CC'TCA_GC Cuts at: 2526 2855 2526 Size: 329 5807 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4327 4361 1903 Size: 34 2390 34 3678 Fragments arranged by size: 3678 2390 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 2112 1548 Size: 564 5572 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4747 136 Size: 107 122 71 631 605 3075 1525 Fragments arranged by size: 3075 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3421 6131 3421 Size: 2710 3426 BlpI GC'TnA_GC Cuts at: 2700 3553 2700 Size: 853 5283 Bme1580I G_kGCm'C Cuts at: 1790 4197 5353 1790 Size: 2407 1156 2573 Fragments arranged by size: 2573 2407 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6136 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3867 4825 442 Size: 416 53 83 399 24 2450 958 1753 Fragments arranged by size: 2450 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3351 3351 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2390 2864 2885 4796 1239 Size: 809 342 474 21 1911 2579 Fragments arranged by size: 2579 1911 809 474 342 21 Bpu10I CC'TnA_GC Cuts at: 2064 2526 2855 2064 Size: 462 329 5345 Fragments arranged by size: 5345 462 329 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4261 5126 5424 4261 Size: 865 298 4973 Fragments arranged by size: 4973 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4799 882 Size: 617 3300 2219 Fragments arranged by size: 3300 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2831 3433 3927 493 Size: 711 1627 602 494 2702 Fragments arranged by size: 2702 1627 711 602 494 BsaBI GATnn'nnATC Cuts at: 3291 6130 3291 Size: 2839 3297 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3943 4325 4960 275 Size: 53 83 186 936 2410 382 635 1451 Fragments arranged by size: 2410 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4569 4967 5241 5388 5799 4569 Size: 398 274 147 411 4906 Fragments arranged by size: 4906 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5911 Fragments arranged by size: 5911 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3023 1186 Size: 1837 4299 BseYI C'CCAG_C Cuts at: 2279 5339 5835 2279 Size: 3060 496 2580 Fragments arranged by size: 3060 2580 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2811 1163 Size: 573 1075 4488 Fragments arranged by size: 4488 1075 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3586 4347 5375 1540 Size: 275 186 1585 761 1028 2301 Fragments arranged by size: 2301 1585 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4197 4282 5353 4197 Size: 85 1071 4980 Fragments arranged by size: 4980 1071 85 BsmI GAATG_Cn' Cuts at: 2856 2982 3647 3740 2856 Size: 126 665 93 5252 Fragments arranged by size: 5252 665 126 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3455 4197 4282 5353 1391 Size: 399 1665 742 85 1071 2174 Fragments arranged by size: 2174 1665 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5799 5799 Size: 6136 BspHI T'CATG_A Cuts at: 4025 5755 4025 Size: 1730 4406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2769 3087 3491 844 Size: 1925 318 404 3489 Fragments arranged by size: 3489 1925 404 318 BsrBI CCG'CTC Cuts at: 1394 1823 4023 1394 Size: 429 2200 3507 Fragments arranged by size: 3507 2200 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4631 4805 65 Size: 1183 3383 174 1396 Fragments arranged by size: 3383 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4780 4967 1700 Size: 110 2970 187 2869 Fragments arranged by size: 2970 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6136 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6136 BssSI C'ACGA_G Cuts at: 4196 5208 4196 Size: 1012 5124 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6136 BstBI TT'CG_AA Cuts at: 2007 4940 2007 Size: 2933 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6136 BstYI r'GATC_y Cuts at: 1972 2311 3421 3466 3833 4218 4235 5687 5773 Size: 339 1110 45 367 385 17 1452 86 Cuts at: 5773 5785 6131 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1110 385 367 346 339 86 45 17 12 BstZ17I GTA'TAC Cuts at: 3199 3199 Size: 6136 BtgI C'CryG_G Cuts at: 513 1065 2909 3352 513 Size: 552 1844 443 3297 Fragments arranged by size: 3297 1844 552 443 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5397 Fragments arranged by size: 5397 683 56 BtsI GCAGTG_nn' Cuts at: 2356 2823 3652 4446 4466 2356 Size: 467 829 794 20 4026 Fragments arranged by size: 4026 829 794 467 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6101 DraI TTT'AAA Cuts at: 3085 3440 3085 Size: 355 5781 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5143 809 Size: 507 3827 1802 Fragments arranged by size: 3827 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3355 3583 4472 Size: 54 1425 50 275 18 1525 228 889 Cuts at: 4472 6101 8 Size: 1629 43 Fragments arranged by size: 1629 1525 1425 889 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3583 1537 Size: 275 1771 4090 Fragments arranged by size: 4090 1771 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2514 4066 1353 Size: 1161 1552 3423 Fragments arranged by size: 3423 1552 1161 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4723 5097 5243 387 Size: 1064 138 3134 374 146 1280 Fragments arranged by size: 3134 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2390 2864 2885 4199 4796 5583 Size: 393 416 342 474 21 1314 597 787 Cuts at: 5583 5851 1239 Size: 268 1524 Fragments arranged by size: 1524 1314 787 597 474 416 393 342 268 21 EcoO109I rG'GnC_Cy Cuts at: 2758 3109 3331 2758 Size: 351 222 5563 Fragments arranged by size: 5563 351 222 EcoRI G'AATT_C Cuts at: 3210 3445 3823 3210 Size: 235 378 5523 Fragments arranged by size: 5523 378 235 EcoRV GAT'ATC Cuts at: 2434 2434 Size: 6136 FspI TGC'GCA Cuts at: 4642 4642 Size: 6136 HaeII r_GCGC'y Cuts at: 1678 5283 1678 Size: 3605 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4777 4809 4851 4883 1373 Size: 32 135 32 3205 32 42 32 2626 Fragments arranged by size: 3205 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3480 3726 669 Size: 988 281 1542 246 3079 Fragments arranged by size: 3079 1542 988 281 246 HindIII A'AGCT_T Cuts at: 748 2051 2539 748 Size: 1303 488 4345 Fragments arranged by size: 4345 1303 488 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KpnI G_GTAC'C Cuts at: 3461 3461 Size: 6136 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2825 2900 5250 5434 1305 Size: 1520 75 2350 184 2007 Fragments arranged by size: 2350 2007 1520 184 75 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4314 Fragments arranged by size: 4314 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 3389 4094 4453 4612 518 Size: 648 873 1350 705 359 159 2042 Fragments arranged by size: 2042 1350 873 705 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2530 3552 4229 5377 5622 1606 Size: 924 1022 677 1148 245 2120 Fragments arranged by size: 2120 1148 1022 924 677 245 NcoI C'CATG_G Cuts at: 513 1065 2909 3352 513 Size: 552 1844 443 3297 Fragments arranged by size: 3297 1844 552 443 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 3494 5039 1132 Size: 97 2265 1545 2229 Fragments arranged by size: 2265 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6136 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6136 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PmlI CAC'GTG Cuts at: 2831 2831 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 5718 5750 4205 Size: 32 1481 32 4591 Fragments arranged by size: 4591 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2758 3109 3331 2758 Size: 351 222 5563 Fragments arranged by size: 5563 351 222 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6136 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PstI C_TGCA'G Cuts at: 830 3348 3488 830 Size: 2518 140 3478 Fragments arranged by size: 3478 2518 140 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 PvuII CAG'CTG Cuts at: 2530 2530 Size: 6136 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SanDI GG'GwC_CC Cuts at: 3331 3331 Size: 6136 SbfI CC_TGCA'GG Cuts at: 3348 3488 3348 Size: 140 5996 ScaI AGT'ACT Cuts at: 2460 4384 6096 2460 Size: 1924 1712 2500 Fragments arranged by size: 2500 1924 1712 SexAI A'CCwGG_T Cuts at: 3192 3192 Size: 6136 SfcI C'TryA_G Cuts at: 826 1045 2204 3344 3484 4619 5300 5491 826 Size: 219 1159 1140 140 1135 681 191 1471 Fragments arranged by size: 1471 1159 1140 1135 681 219 191 140 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 3463 1872 Size: 1591 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 4240 5141 5403 820 Size: 197 926 786 1511 901 262 1553 Fragments arranged by size: 1553 1511 926 901 786 262 197 SnaBI TAC'GTA Cuts at: 493 3433 3927 493 Size: 2940 494 2702 Fragments arranged by size: 2940 2702 494 SpeI A'CTAG_T Cuts at: 152 2396 152 Size: 2244 3892 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 4060 5 Size: 47 4008 2081 Fragments arranged by size: 4008 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6136 StyI C'CwwG_G Cuts at: 513 1065 2909 3352 3575 513 Size: 552 1844 443 223 3074 Fragments arranged by size: 3074 1844 552 443 223 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2736 4163 4180 4333 4518 4963 325 Size: 1255 1156 1427 17 153 185 445 1498 Fragments arranged by size: 1498 1427 1255 1156 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2458 4382 6094 Size: 274 80 33 51 1443 481 1924 1712 Cuts at: 6094 96 Size: 138 Fragments arranged by size: 1924 1712 1443 481 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6096 6128 6096 Size: 32 6104 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6136 XbaI T'CTAG_A Cuts at: 2534 3472 2534 Size: 938 5198 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2916 2916 Size: 6136 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6136 XmaI C'CCGG_G Cuts at: 1870 3461 1870 Size: 1591 4545 XmnI GAAnn'nnTTC Cuts at: 2052 2134 4265 2052 Size: 82 2131 3923 Fragments arranged by size: 3923 2131 82 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3944 276 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI