(Circular) (Six-base) MAPSORT of: pFN21ASDA0893.seq Check: 6853 from: 1 to: 7486 pFN21ASDA0893 7486 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5296 278 Size: 53 83 186 936 3760 2468 Fragments arranged by size: 3760 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4829 1937 Size: 2892 4594 Acc65I G'GTAC_C Cuts at: 4807 4807 Size: 7486 AclI AA'CG_TT Cuts at: 1563 5613 5986 1563 Size: 4050 373 3063 Fragments arranged by size: 4050 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2052 2201 2303 2750 3702 5549 6933 7201 Size: 420 149 102 447 952 1847 1384 268 Cuts at: 7201 1632 Size: 1917 Fragments arranged by size: 1917 1847 1384 952 447 420 268 149 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7289 AflIII A'CryG_T Cuts at: 1203 6385 7362 1203 Size: 5182 977 1327 Fragments arranged by size: 5182 1327 977 AgeI A'CCGG_T Cuts at: 6317 6317 Size: 7486 AhdI GACnn_n'nnGTC Cuts at: 6215 6215 Size: 7486 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7486 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7486 AlwNI CAG_nnn'CTG Cuts at: 2350 5229 6801 2350 Size: 2879 1572 3035 Fragments arranged by size: 3035 2879 1572 ApaLI G'TGCA_C Cuts at: 3948 5543 6699 3948 Size: 1595 1156 4735 Fragments arranged by size: 4735 1595 1156 ApoI r'AATT_y Cuts at: 1493 2089 2249 2354 2543 2648 3012 3898 4130 Size: 596 160 105 189 105 364 886 232 Cuts at: 4130 4795 5021 5173 7282 1493 Size: 665 226 152 2109 1697 Fragments arranged by size: 2109 1697 886 665 596 364 232 226 189 160 152 105 105 AseI AT'TA_AT Cuts at: 160 1032 3822 6040 160 Size: 872 2790 2218 1606 Fragments arranged by size: 2790 2218 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7486 AvaI C'yCGr_G Cuts at: 1870 1943 4811 1870 Size: 73 2868 4545 Fragments arranged by size: 4545 2868 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3147 3180 3147 Size: 33 7453 BamHI G'GATC_C Cuts at: 4007 4816 5183 4007 Size: 809 367 6310 Fragments arranged by size: 6310 809 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4807 6262 618 Size: 325 217 627 3020 1455 1842 Fragments arranged by size: 3020 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2343 4585 4805 1391 Size: 952 2242 220 4072 Fragments arranged by size: 4072 2242 952 220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2779 2929 3840 928 Size: 971 880 150 911 4574 Fragments arranged by size: 4574 971 911 880 150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5677 5711 1903 Size: 34 3740 34 3678 Fragments arranged by size: 3740 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5378 6588 5378 Size: 1210 6276 BclI T'GATC_A Cuts at: 1548 3418 1548 Size: 1870 5616 BfrBI ATG'CAT Cuts at: 4141 4141 Size: 7486 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6097 136 Size: 107 122 71 631 605 4425 1525 Fragments arranged by size: 4425 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4123 7481 4123 Size: 3358 4128 BlpI GC'TnA_GC Cuts at: 4903 4903 Size: 7486 Bme1580I G_kGCm'C Cuts at: 1790 3952 5547 6703 1790 Size: 2162 1595 1156 2573 Fragments arranged by size: 2573 2162 1595 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7486 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4296 4446 5217 Size: 416 53 83 399 24 2879 150 771 Cuts at: 5217 6175 442 Size: 958 1753 Fragments arranged by size: 2879 1753 958 771 416 399 150 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7486 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2943 2943 Size: 7486 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2691 3707 3741 3894 4048 4233 4333 Size: 905 547 1016 34 153 154 185 100 Cuts at: 4333 4599 6146 1239 Size: 266 1547 2579 Fragments arranged by size: 2579 1547 1016 905 547 266 185 154 153 100 34 Bpu10I CC'TnA_GC Cuts at: 3147 3963 3147 Size: 816 6670 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3128 5611 6476 6774 3128 Size: 2483 865 298 3840 Fragments arranged by size: 3840 2483 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6149 882 Size: 617 4650 2219 Fragments arranged by size: 4650 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4783 5277 493 Size: 711 3579 494 2702 Fragments arranged by size: 3579 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2742 7480 2742 Size: 4738 2748 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5293 5675 6310 275 Size: 53 83 186 936 3760 382 635 1451 Fragments arranged by size: 3760 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5919 6317 6591 6738 7149 5919 Size: 398 274 147 411 6256 Fragments arranged by size: 6256 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3631 3661 4021 4051 1178 Size: 30 165 30 2228 30 360 30 4613 Fragments arranged by size: 4613 2228 360 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2868 3639 4206 4527 1186 Size: 1682 771 567 321 4145 Fragments arranged by size: 4145 1682 771 567 321 BseYI C'CCAG_C Cuts at: 2343 2492 6689 7185 2343 Size: 149 4197 496 2644 Fragments arranged by size: 4197 2644 496 149 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4446 4723 1163 Size: 573 2710 277 3926 Fragments arranged by size: 3926 2710 573 277 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4936 5697 6725 1540 Size: 275 186 2935 761 1028 2301 Fragments arranged by size: 2935 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3241 3952 4585 4683 5547 5632 6703 3241 Size: 711 633 98 864 85 1071 4024 Fragments arranged by size: 4024 1071 864 711 633 98 85 BsmI GAATG_Cn' Cuts at: 2642 4997 5090 2642 Size: 2355 93 5038 Fragments arranged by size: 5038 2355 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4466 4466 Size: 7486 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2343 3241 3952 4585 4683 4805 5547 Size: 399 553 898 711 633 98 122 742 Cuts at: 5547 5632 6703 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 898 742 711 633 553 399 122 98 85 BspEI T'CCGG_A Cuts at: 7149 7149 Size: 7486 BspHI T'CATG_A Cuts at: 3001 5375 7105 3001 Size: 2374 1730 3382 Fragments arranged by size: 3382 2374 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4841 844 Size: 3997 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2955 5373 1394 Size: 429 1132 2418 3507 Fragments arranged by size: 3507 2418 1132 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2384 3536 3556 5981 6155 65 Size: 1183 1136 1152 20 2425 174 1396 Fragments arranged by size: 2425 1396 1183 1152 1136 174 20 BsrFI r'CCGG_y Cuts at: 1700 1810 3970 6130 6317 1700 Size: 110 2160 2160 187 2869 Fragments arranged by size: 2869 2160 2160 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7486 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7486 BssSI C'ACGA_G Cuts at: 2502 5546 6558 2502 Size: 3044 1012 3430 Fragments arranged by size: 3430 3044 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7486 BstBI TT'CG_AA Cuts at: 2007 6290 2007 Size: 4283 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7486 BstYI r'GATC_y Cuts at: 1972 2743 3554 4007 4123 4366 4447 4720 4816 Size: 771 811 453 116 243 81 273 96 Cuts at: 4816 5183 5568 5585 7037 7123 7135 7481 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 811 771 453 385 367 346 273 243 116 96 86 81 17 12 Bsu36I CC'TnA_GG Cuts at: 2177 2177 Size: 7486 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6934 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2336 487 Size: 683 56 1110 5637 Fragments arranged by size: 5637 1110 683 56 BtsI GCAGTG_nn' Cuts at: 3193 3861 4429 4434 5002 5796 5816 3193 Size: 668 568 5 568 794 20 4863 Fragments arranged by size: 4863 794 668 568 568 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7486 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7451 DraI TTT'AAA Cuts at: 3985 4790 3985 Size: 805 6681 DraIII CAC_nnn'GTG Cuts at: 3701 3701 Size: 7486 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2997 4073 6493 809 Size: 507 1681 1076 2420 1802 Fragments arranged by size: 2420 1802 1681 1076 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4933 5822 7451 Size: 54 1425 50 275 18 3103 889 1629 Cuts at: 7451 8 Size: 43 Fragments arranged by size: 3103 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4933 1537 Size: 275 3121 4090 Fragments arranged by size: 4090 3121 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2514 3721 3780 5416 1353 Size: 1161 1207 59 1636 3423 Fragments arranged by size: 3423 1636 1207 1161 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6073 6447 6593 387 Size: 1064 138 4484 374 146 1280 Fragments arranged by size: 4484 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4583 4583 Size: 7486 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 2144 2201 2303 2691 2750 3702 Size: 393 420 92 57 102 388 59 952 Cuts at: 3702 3707 3741 3894 4048 4233 4333 4599 5549 Size: 5 34 153 154 185 100 266 950 Cuts at: 5549 6146 6933 7201 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 952 950 787 597 420 393 388 268 266 185 154 153 102 100 92 59 57 34 5 EcoNI CCTnn'n_nnAGG Cuts at: 2126 2126 Size: 7486 EcoO109I rG'GnC_Cy Cuts at: 4261 4642 4261 Size: 381 7105 EcoRI G'AATT_C Cuts at: 2089 2543 4795 5173 2089 Size: 454 2252 378 4402 Fragments arranged by size: 4402 2252 454 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2645 2717 2645 Size: 72 7414 FspI TGC'GCA Cuts at: 5992 5992 Size: 7486 HaeII r_GCGC'y Cuts at: 1678 6633 1678 Size: 4955 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2437 2469 2941 2973 3782 Size: 32 135 32 865 32 472 32 809 Cuts at: 3782 3814 4544 4576 6127 6159 6201 6233 1373 Size: 32 730 32 1551 32 42 32 2626 Fragments arranged by size: 2626 1551 865 809 730 472 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2314 2830 4830 5076 669 Size: 988 281 376 516 2000 246 3079 Fragments arranged by size: 3079 2000 988 516 376 281 246 HindIII A'AGCT_T Cuts at: 748 2099 2301 3467 748 Size: 1351 202 1166 4767 Fragments arranged by size: 4767 1351 1166 202 HpaI GTT'AAC Cuts at: 2314 5076 2314 Size: 2762 4724 KpnI G_GTAC'C Cuts at: 4811 4811 Size: 7486 MfeI C'AATT_G Cuts at: 5085 5085 Size: 7486 MluI A'CGCG_T Cuts at: 7362 7362 Size: 7486 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6600 6784 1305 Size: 5295 184 2007 Fragments arranged by size: 5295 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5664 Fragments arranged by size: 5664 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 4548 4670 5444 5803 5962 518 Size: 648 945 2437 122 774 359 159 2042 Fragments arranged by size: 2437 2042 945 774 648 359 159 122 MspA1I CmG'CkG Cuts at: 1606 2936 3361 3872 4902 5579 6727 6972 1606 Size: 1330 425 511 1030 677 1148 245 2120 Fragments arranged by size: 2120 1330 1148 1030 677 511 425 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6934 NdeI CA'TA_TG Cuts at: 387 2860 387 Size: 2473 5013 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7486 NotI GC'GGCC_GC Cuts at: 4933 4933 Size: 7486 NsiI A_TGCA'T Cuts at: 4143 4143 Size: 7486 NspI r_CATG'y Cuts at: 1132 1229 2390 4498 4844 6389 1132 Size: 97 1161 2108 346 1545 2229 Fragments arranged by size: 2229 2108 1545 1161 346 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7486 PciI A'CATG_T Cuts at: 6385 6385 Size: 7486 PflMI CCAn_nnn'nTGG Cuts at: 1108 2350 3746 1108 Size: 1242 1396 4848 Fragments arranged by size: 4848 1396 1242 PfoI T'CCnGG_A Cuts at: 3247 3658 3247 Size: 411 7075 PmeI GTTT'AAAC Cuts at: 4790 4790 Size: 7486 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5555 5587 7068 7100 5555 Size: 32 1481 32 5941 Fragments arranged by size: 5941 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4642 4642 Size: 7486 PshAI GACnn'nnGTC Cuts at: 1613 3603 1613 Size: 1990 5496 PsiI TTA'TAA Cuts at: 5056 5056 Size: 7486 PstI C_TGCA'G Cuts at: 830 4747 4838 830 Size: 3917 91 3478 Fragments arranged by size: 3917 3478 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7486 PvuII CAG'CTG Cuts at: 2936 3872 2936 Size: 936 6550 SacI G_AGCT'C Cuts at: 4585 4585 Size: 7486 SalI G'TCGA_C Cuts at: 1936 4828 1936 Size: 2892 4594 SbfI CC_TGCA'GG Cuts at: 4838 4838 Size: 7486 ScaI AGT'ACT Cuts at: 5734 7446 5734 Size: 1712 5774 SfcI C'TryA_G Cuts at: 826 1045 2422 4437 4443 4743 4834 5969 6650 Size: 219 1377 2015 6 300 91 1135 681 Cuts at: 6650 6841 826 Size: 191 1471 Fragments arranged by size: 2015 1471 1377 1135 681 300 219 191 91 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7486 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7486 SmaI CCC'GGG Cuts at: 1872 4813 1872 Size: 2941 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3107 5590 6491 6753 820 Size: 197 926 1164 2483 901 262 1553 Fragments arranged by size: 2483 1553 1164 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4783 5277 493 Size: 4290 494 2702 Fragments arranged by size: 4290 2702 494 SpeI A'CTAG_T Cuts at: 152 3575 152 Size: 3423 4063 SphI G_CATG'C Cuts at: 1132 4844 1132 Size: 3712 3774 SspI AAT'ATT Cuts at: 5 52 2821 3308 3484 3634 5410 5 Size: 47 2769 487 176 150 1776 2081 Fragments arranged by size: 2769 2081 1776 487 176 150 47 StuI AGG'CCT Cuts at: 1517 2625 3178 1517 Size: 1108 553 5825 Fragments arranged by size: 5825 1108 553 StyI C'CwwG_G Cuts at: 513 1065 3022 3499 4925 513 Size: 552 1957 477 1426 3074 Fragments arranged by size: 3074 1957 1426 552 477 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3718 4730 5513 5530 5683 5868 6313 Size: 1255 2138 1012 783 17 153 185 445 Cuts at: 6313 325 Size: 1498 Fragments arranged by size: 2138 1498 1255 1012 783 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5732 7444 96 Size: 274 80 33 51 1443 3755 1712 138 Fragments arranged by size: 3755 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2554 2586 4021 4053 7446 7478 2554 Size: 32 1435 32 3393 32 2562 Fragments arranged by size: 3393 2562 1435 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7486 XbaI T'CTAG_A Cuts at: 2233 2392 4822 2233 Size: 159 2430 4897 Fragments arranged by size: 4897 2430 159 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3533 4018 4274 3533 Size: 485 256 6745 Fragments arranged by size: 6745 485 256 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7486 XmaI C'CCGG_G Cuts at: 1870 4811 1870 Size: 2941 4545 XmnI GAAnn'nnTTC Cuts at: 2214 2697 3297 5615 2214 Size: 483 600 2318 4085 Fragments arranged by size: 4085 2318 600 483 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5294 276 Size: 53 83 186 936 3760 2468 Fragments arranged by size: 3760 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI