Restriction map for
pFN21ASDA0893
(Linear) (Six-base) MAPSORT of: KIAA0893.seq Check: 106 from: 1 to: 2757
KIAA0893.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 178 280 727 1679 2757
Size: 29 149 102 447 952 1078
Fragments arranged by size:
1078 952 447 149 102 29
AlwNI CAG_nnn'CTG
Cuts at: 0 327 2757
Size: 327 2430
ApaLI G'TGCA_C
Cuts at: 0 1925 2757
Size: 1925 832
ApoI r'AATT_y
Cuts at: 0 66 226 331 520 625 989 1875 2107
Size: 66 160 105 189 105 364 886 232
Cuts at: 2107 2757
Size: 650
Fragments arranged by size:
886 650 364 232 189 160 105 105
66
AseI AT'TA_AT
Cuts at: 0 1799 2757
Size: 1799 958
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1124 1157 2757
Size: 1124 33 1600
Fragments arranged by size:
1600 1124 33
BamHI G'GATC_C
Cuts at: 0 1984 2757
Size: 1984 773
BanII G_rGCy'C
Cuts at: 0 320 2562 2757
Size: 320 2242 195
Fragments arranged by size:
2242 320 195
BbsI GAAGACnn'nnnn_
Cuts at: 0 756 906 1817 2757
Size: 756 150 911 940
Fragments arranged by size:
940 911 756 150
BclI T'GATC_A
Cuts at: 0 1395 2757
Size: 1395 1362
BfrBI ATG'CAT
Cuts at: 0 2118 2757
Size: 2118 639
BglII A'GATC_T
Cuts at: 0 2100 2757
Size: 2100 657
Bme1580I G_kGCm'C
Cuts at: 0 1929 2757
Size: 1929 828
BmrI ACTGGGnnnn_n'
Cuts at: 0 2273 2423 2757
Size: 2273 150 334
Fragments arranged by size:
2273 334 150
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 920 2757
Size: 920 1837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 668 1684 1718 1871 2025 2210 2310
Size: 121 547 1016 34 153 154 185 100
Cuts at: 2310 2576 2757
Size: 266 181
Fragments arranged by size:
1016 547 266 185 181 154 153 121
100 34
Bpu10I CC'TnA_GC
Cuts at: 0 1124 1940 2757
Size: 1124 816 817
Fragments arranged by size:
1124 817 816
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1105 2757
Size: 1105 1652
BsaBI GATnn'nnATC
Cuts at: 0 719 2757
Size: 719 2038
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1608 1638 1998 2028 2757
Size: 1608 30 360 30 729
Fragments arranged by size:
1608 729 360 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 845 1616 2183 2504 2757
Size: 845 771 567 321 253
Fragments arranged by size:
845 771 567 321 253
BseYI C'CCAG_C
Cuts at: 0 320 469 2757
Size: 320 149 2288
Fragments arranged by size:
2288 320 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2423 2700 2757
Size: 2423 277 57
Fragments arranged by size:
2423 277 57
BsiHKAI G_wGCw'C
Cuts at: 0 1218 1929 2562 2660 2757
Size: 1218 711 633 98 97
Fragments arranged by size:
1218 711 633 98 97
BsmI GAATG_Cn'
Cuts at: 0 619 2757
Size: 619 2138
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2443 2757
Size: 2443 314
Bsp1286I G_dGCh'C
Cuts at: 0 320 1218 1929 2562 2660 2757
Size: 320 898 711 633 98 97
Fragments arranged by size:
898 711 633 320 98 97
BspHI T'CATG_A
Cuts at: 0 978 2757
Size: 978 1779
BsrBI CCG'CTC
Cuts at: 0 932 2757
Size: 932 1825
BsrDI GCAATG_nn'
Cuts at: 0 361 1513 1533 2757
Size: 361 1152 20 1224
Fragments arranged by size:
1224 1152 361 20
BsrFI r'CCGG_y
Cuts at: 0 1947 2757
Size: 1947 810
BssSI C'ACGA_G
Cuts at: 0 479 2757
Size: 479 2278
BstYI r'GATC_y
Cuts at: 0 720 1531 1984 2100 2343 2424 2697 2757
Size: 720 811 453 116 243 81 273 60
Fragments arranged by size:
811 720 453 273 243 116 81 60
Bsu36I CC'TnA_GG
Cuts at: 0 154 2757
Size: 154 2603
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 313 2757
Size: 313 2444
BtsI GCAGTG_nn'
Cuts at: 0 1170 1838 2406 2411 2757
Size: 1170 668 568 5 346
Fragments arranged by size:
1170 668 568 346 5
DraI TTT'AAA
Cuts at: 0 1962 2757
Size: 1962 795
DraIII CAC_nnn'GTG
Cuts at: 0 1678 2757
Size: 1678 1079
DrdI GACnn_nn'nnGTC
Cuts at: 0 974 2050 2757
Size: 974 1076 707
Fragments arranged by size:
1076 974 707
EarI CTCTTCn'nnn_
Cuts at: 0 491 1698 1757 2757
Size: 491 1207 59 1000
Fragments arranged by size:
1207 1000 491 59
EcoICRI GAG'CTC
Cuts at: 0 2560 2757
Size: 2560 197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 121 178 280 668 727 1679 1684
Size: 29 92 57 102 388 59 952 5
Cuts at: 1684 1718 1871 2025 2210 2310 2576 2757
Size: 34 153 154 185 100 266 181
Fragments arranged by size:
952 388 266 185 181 154 153 102
100 92 59 57 34 29 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 103 2757
Size: 103 2654
EcoO109I rG'GnC_Cy
Cuts at: 0 2238 2619 2757
Size: 2238 381 138
Fragments arranged by size:
2238 381 138
EcoRI G'AATT_C
Cuts at: 0 66 520 2757
Size: 66 454 2237
Fragments arranged by size:
2237 454 66
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 622 694 2757
Size: 622 72 2063
Fragments arranged by size:
2063 622 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 414 446 918 950 1759 1791 2521 2553
Size: 414 32 472 32 809 32 730 32
Cuts at: 2553 2757
Size: 204
Fragments arranged by size:
809 730 472 414 204 32 32 32
32
HincII GTy'rAC
Cuts at: 0 291 807 2757
Size: 291 516 1950
Fragments arranged by size:
1950 516 291
HindIII A'AGCT_T
Cuts at: 0 76 278 1444 2757
Size: 76 202 1166 1313
Fragments arranged by size:
1313 1166 202 76
HpaI GTT'AAC
Cuts at: 0 291 2757
Size: 291 2466
MslI CAynn'nnrTG
Cuts at: 0 88 2525 2647 2757
Size: 88 2437 122 110
Fragments arranged by size:
2437 122 110 88
MspA1I CmG'CkG
Cuts at: 0 913 1338 1849 2757
Size: 913 425 511 908
Fragments arranged by size:
913 908 511 425
NdeI CA'TA_TG
Cuts at: 0 837 2757
Size: 837 1920
NsiI A_TGCA'T
Cuts at: 0 2120 2757
Size: 2120 637
NspI r_CATG'y
Cuts at: 0 367 2475 2757
Size: 367 2108 282
Fragments arranged by size:
2108 367 282
PflMI CCAn_nnn'nTGG
Cuts at: 0 327 1723 2757
Size: 327 1396 1034
Fragments arranged by size:
1396 1034 327
PfoI T'CCnGG_A
Cuts at: 0 1224 1635 2757
Size: 1224 411 1122
Fragments arranged by size:
1224 1122 411
PpuMI rG'GwC_Cy
Cuts at: 0 2619 2757
Size: 2619 138
PshAI GACnn'nnGTC
Cuts at: 0 1580 2757
Size: 1580 1177
PstI C_TGCA'G
Cuts at: 0 2724 2757
Size: 2724 33
PvuII CAG'CTG
Cuts at: 0 913 1849 2757
Size: 913 936 908
Fragments arranged by size:
936 913 908
SacI G_AGCT'C
Cuts at: 0 2562 2757
Size: 2562 195
SfcI C'TryA_G
Cuts at: 0 399 2414 2420 2720 2757
Size: 399 2015 6 300 37
Fragments arranged by size:
2015 399 300 37 6
SmlI C'TyrA_G
Cuts at: 0 1084 2757
Size: 1084 1673
SpeI A'CTAG_T
Cuts at: 0 1552 2757
Size: 1552 1205
SspI AAT'ATT
Cuts at: 0 798 1285 1461 1611 2757
Size: 798 487 176 150 1146
Fragments arranged by size:
1146 798 487 176 150
StuI AGG'CCT
Cuts at: 0 602 1155 2757
Size: 602 553 1602
Fragments arranged by size:
1602 602 553
StyI C'CwwG_G
Cuts at: 0 999 1476 2757
Size: 999 477 1281
Fragments arranged by size:
1281 999 477
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1695 2707 2757
Size: 1695 1012 50
Fragments arranged by size:
1695 1012 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 531 563 1998 2030 2757
Size: 531 32 1435 32 727
Fragments arranged by size:
1435 727 531 32 32
XbaI T'CTAG_A
Cuts at: 0 210 369 2757
Size: 210 159 2388
Fragments arranged by size:
2388 210 159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1510 1995 2251 2757
Size: 1510 485 256 506
Fragments arranged by size:
1510 506 485 256
XmnI GAAnn'nnTTC
Cuts at: 0 191 674 1274 2757
Size: 191 483 600 1483
Fragments arranged by size:
1483 600 483 191
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI AseI BaeI BamHI BanII
BbsI BclI BfrBI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BssSI
BstYI Bsu36I BtgZI BtsI DraI DraIII DrdI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII
HindIII HpaI MslI MspA1I NdeI NsiI NspI PflMI
PfoI PpuMI PshAI PstI PvuII SacI SfcI SmlI
SpeI SspI StuI StyI TaqII TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AsiSI
AvaI AvrII BanI BbeI BbvCI BcgI BciVI BglI
BlpI BmgBI BmtI BsaI BsaAI BsaHI BsaWI BsiEI
BsiWI BspEI BspMI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I BtgI ClaI CspCI EaeI EagI EciI
EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PciI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI TatI Tth111I
XhoI XmaI ZraI