(Circular) (Six-base) MAPSORT of: pFN21ASDA0888.seq Check: 5541 from: 1 to: 6739 pFN21ASDA0888 6739 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2329 2329 Size: 6739 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4549 278 Size: 53 83 186 936 3013 2468 Fragments arranged by size: 3013 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2687 3134 3496 3963 4082 1937 Size: 750 447 362 467 119 4594 Fragments arranged by size: 4594 750 467 447 362 119 Acc65I G'GTAC_C Cuts at: 2766 4060 2766 Size: 1294 5445 AclI AA'CG_TT Cuts at: 1563 4866 5239 1563 Size: 3303 373 3063 Fragments arranged by size: 3303 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2103 2259 2922 3888 4802 6186 6454 1632 Size: 471 156 663 966 914 1384 268 1917 Fragments arranged by size: 1917 1384 966 914 663 471 268 156 AflII C'TTAA_G Cuts at: 820 1017 2333 3329 820 Size: 197 1316 996 4230 Fragments arranged by size: 4230 1316 996 197 AflIII A'CryG_T Cuts at: 1203 2091 5638 6615 1203 Size: 888 3547 977 1327 Fragments arranged by size: 3547 1327 977 888 AgeI A'CCGG_T Cuts at: 3685 5570 3685 Size: 1885 4854 AhdI GACnn_n'nnGTC Cuts at: 2911 5468 2911 Size: 2557 4182 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6739 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6739 AlwNI CAG_nnn'CTG Cuts at: 4482 6054 4482 Size: 1572 5167 ApaLI G'TGCA_C Cuts at: 4796 5952 4796 Size: 1156 5583 ApoI r'AATT_y Cuts at: 1493 2074 2217 2814 3059 3164 3200 3671 3697 Size: 581 143 597 245 105 36 471 26 Cuts at: 3697 3973 4048 4274 4426 6535 1493 Size: 276 75 226 152 2109 1697 Fragments arranged by size: 2109 1697 597 581 471 276 245 226 152 143 105 75 36 26 AseI AT'TA_AT Cuts at: 160 1032 5293 160 Size: 872 4261 1606 Fragments arranged by size: 4261 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 AvaI C'yCGr_G Cuts at: 1870 1943 4064 1870 Size: 73 2121 4545 Fragments arranged by size: 4545 2121 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2750 2783 2750 Size: 33 6706 BamHI G'GATC_C Cuts at: 4069 4436 4069 Size: 367 6372 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2766 4060 5515 618 Size: 325 217 627 979 1294 1455 1842 Fragments arranged by size: 1842 1455 1294 979 627 325 217 BanII G_rGCy'C Cuts at: 1391 3158 3759 4058 1391 Size: 1767 601 299 4072 Fragments arranged by size: 4072 1767 601 299 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2667 2910 3363 3423 3735 3819 928 Size: 971 768 243 453 60 312 84 3848 Fragments arranged by size: 3848 971 768 453 312 243 84 60 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4930 4964 1903 Size: 34 2993 34 3678 Fragments arranged by size: 3678 2993 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2676 2727 4631 5841 2676 Size: 51 1904 1210 3574 Fragments arranged by size: 3574 1904 1210 51 BclI T'GATC_A Cuts at: 1548 2275 1548 Size: 727 6012 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5350 136 Size: 107 122 71 631 605 3678 1525 Fragments arranged by size: 3678 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6734 6734 Size: 6739 BlpI GC'TnA_GC Cuts at: 4023 4156 4023 Size: 133 6606 Bme1580I G_kGCm'C Cuts at: 1790 3549 4800 5956 1790 Size: 1759 1251 1156 2573 Fragments arranged by size: 2573 1759 1251 1156 BmgBI CAC'GTC Cuts at: 1318 3817 1318 Size: 2499 4240 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4470 5428 442 Size: 416 53 83 399 24 3053 958 1753 Fragments arranged by size: 3053 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6739 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3376 3376 Size: 6739 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2639 2877 5399 1239 Size: 1400 238 2522 2579 Fragments arranged by size: 2579 2522 1400 238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3057 4864 5729 6027 3057 Size: 1807 865 298 3769 Fragments arranged by size: 3769 1807 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2718 3694 5402 882 Size: 617 1219 976 1708 2219 Fragments arranged by size: 2219 1708 1219 976 617 BsaAI yAC'GTr Cuts at: 493 1204 3494 4036 4530 493 Size: 711 2290 542 494 2702 Fragments arranged by size: 2702 2290 711 542 494 BsaBI GATnn'nnATC Cuts at: 6733 6733 Size: 6739 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4546 4928 5563 275 Size: 53 83 186 936 3013 382 635 1451 Fragments arranged by size: 3013 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3685 5172 5570 5844 5991 6402 3685 Size: 1487 398 274 147 411 4022 Fragments arranged by size: 4022 1487 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6514 Fragments arranged by size: 6514 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3755 3872 1186 Size: 2569 117 4053 Fragments arranged by size: 4053 2569 117 BseYI C'CCAG_C Cuts at: 3249 5942 6438 3249 Size: 2693 496 3550 Fragments arranged by size: 3550 2693 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3123 1163 Size: 573 1387 4779 Fragments arranged by size: 4779 1387 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4189 4950 5978 1540 Size: 275 186 2188 761 1028 2301 Fragments arranged by size: 2301 2188 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2348 3158 4800 4885 5956 2348 Size: 810 1642 85 1071 3131 Fragments arranged by size: 3131 1642 1071 810 85 BsmI GAATG_Cn' Cuts at: 2027 3838 4250 4343 2027 Size: 1811 412 93 4423 Fragments arranged by size: 4423 1811 412 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2348 3158 3549 3759 4058 4800 4885 Size: 399 558 810 391 210 299 742 85 Cuts at: 4885 5956 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 810 742 558 399 391 299 210 85 BspEI T'CCGG_A Cuts at: 6402 6402 Size: 6739 BspHI T'CATG_A Cuts at: 2977 4628 6358 2977 Size: 1651 1730 3358 Fragments arranged by size: 3358 1730 1651 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2329 4015 4094 844 Size: 1485 1686 79 3489 Fragments arranged by size: 3489 1686 1485 79 BsrBI CCG'CTC Cuts at: 1394 1823 4626 1394 Size: 429 2803 3507 Fragments arranged by size: 3507 2803 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5234 5408 65 Size: 1183 3986 174 1396 Fragments arranged by size: 3986 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3685 5383 5570 1700 Size: 110 1875 1698 187 2869 Fragments arranged by size: 2869 1875 1698 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6739 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6739 BssSI C'ACGA_G Cuts at: 4799 5811 4799 Size: 1012 5727 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6739 BstBI TT'CG_AA Cuts at: 2007 5543 2007 Size: 3536 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6739 BstYI r'GATC_y Cuts at: 1972 2421 4069 4436 4821 4838 6290 6376 6388 Size: 449 1648 367 385 17 1452 86 12 Cuts at: 6388 6734 1972 Size: 346 1977 Fragments arranged by size: 1977 1648 1452 449 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2688 3497 2688 Size: 809 5930 Bsu36I CC'TnA_GG Cuts at: 3465 3465 Size: 6739 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5230 Fragments arranged by size: 5230 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6000 Fragments arranged by size: 6000 683 56 BtsI GCAGTG_nn' Cuts at: 3223 4255 5049 5069 3223 Size: 1032 794 20 4893 Fragments arranged by size: 4893 1032 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6739 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6704 DraI TTT'AAA Cuts at: 4043 4043 Size: 6739 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5746 809 Size: 507 4430 1802 Fragments arranged by size: 4430 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3026 3233 4186 Size: 54 1425 50 275 18 1196 207 953 Cuts at: 4186 5075 6704 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1196 953 889 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4186 1537 Size: 275 2374 4090 Fragments arranged by size: 4090 2374 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 3345 3549 3783 4669 1353 Size: 792 1200 204 234 886 3423 Fragments arranged by size: 3423 1200 886 792 234 204 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3985 5326 5700 5846 387 Size: 1064 138 2396 1341 374 146 1280 Fragments arranged by size: 2396 1341 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3156 3156 Size: 6739 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2103 2259 2639 2877 2922 3888 4802 Size: 393 471 156 380 238 45 966 914 Cuts at: 4802 5399 6186 6454 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 966 914 787 597 471 393 380 268 238 156 45 EcoO109I rG'GnC_Cy Cuts at: 2113 4004 2113 Size: 1891 4848 EcoRI G'AATT_C Cuts at: 3164 4048 4426 3164 Size: 884 378 5477 Fragments arranged by size: 5477 884 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2669 2722 3608 2669 Size: 53 886 5800 Fragments arranged by size: 5800 886 53 FspI TGC'GCA Cuts at: 5245 5245 Size: 6739 HaeII r_GCGC'y Cuts at: 1678 5886 1678 Size: 4208 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2897 2929 2972 Size: 32 135 32 506 32 787 32 43 Cuts at: 2972 3004 3548 3580 3686 3718 3734 3766 3848 Size: 32 544 32 106 32 16 32 82 Cuts at: 3848 3880 3914 3946 5380 5412 5454 5486 1373 Size: 32 34 32 1434 32 42 32 2626 Fragments arranged by size: 2626 1434 787 544 506 135 106 82 43 42 34 32 32 32 32 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 4083 4329 669 Size: 988 281 2145 246 3079 Fragments arranged by size: 3079 2145 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6739 HpaI GTT'AAC Cuts at: 4329 4329 Size: 6739 KpnI G_GTAC'C Cuts at: 2770 4064 2770 Size: 1294 5445 MfeI C'AATT_G Cuts at: 2549 4338 2549 Size: 1789 4950 MluI A'CGCG_T Cuts at: 6615 6615 Size: 6739 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2737 5853 6037 1305 Size: 1432 3116 184 2007 Fragments arranged by size: 3116 2007 1432 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4917 Fragments arranged by size: 4917 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2431 4697 5056 5215 518 Size: 648 1265 2266 359 159 2042 Fragments arranged by size: 2266 2042 1265 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2255 4155 4832 5980 6225 1606 Size: 649 1900 677 1148 245 2120 Fragments arranged by size: 2120 1900 1148 677 649 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5230 Fragments arranged by size: 5230 957 552 NdeI CA'TA_TG Cuts at: 387 2519 387 Size: 2132 4607 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6739 NotI GC'GGCC_GC Cuts at: 4186 4186 Size: 6739 NspI r_CATG'y Cuts at: 1132 1229 2095 2617 4097 5642 1132 Size: 97 866 522 1480 1545 2229 Fragments arranged by size: 2229 1545 1480 866 522 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6739 PciI A'CATG_T Cuts at: 2091 5638 2091 Size: 3547 3192 PflMI CCAn_nnn'nTGG Cuts at: 1108 3035 1108 Size: 1927 4812 PfoI T'CCnGG_A Cuts at: 2719 3168 2719 Size: 449 6290 PmeI GTTT'AAAC Cuts at: 4043 4043 Size: 6739 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4808 4840 6321 6353 4808 Size: 32 1481 32 5194 Fragments arranged by size: 5194 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2113 4004 2113 Size: 1891 4848 PshAI GACnn'nnGTC Cuts at: 1613 2851 1613 Size: 1238 5501 PsiI TTA'TAA Cuts at: 3344 4309 3344 Size: 965 5774 PstI C_TGCA'G Cuts at: 830 4091 830 Size: 3261 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6739 PvuII CAG'CTG Cuts at: 2255 2255 Size: 6739 SacI G_AGCT'C Cuts at: 3158 3158 Size: 6739 SalI G'TCGA_C Cuts at: 1936 4081 1936 Size: 2145 4594 SanDI GG'GwC_CC Cuts at: 2113 2113 Size: 6739 SapI GCTCTTCn'nnn_ Cuts at: 3783 3783 Size: 6739 SbfI CC_TGCA'GG Cuts at: 4091 4091 Size: 6739 ScaI AGT'ACT Cuts at: 2446 4987 6699 2446 Size: 2541 1712 2486 Fragments arranged by size: 2541 2486 1712 SfcI C'TryA_G Cuts at: 826 1045 2508 4087 5222 5903 6094 826 Size: 219 1463 1579 1135 681 191 1471 Fragments arranged by size: 1579 1471 1463 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6739 SmaI CCC'GGG Cuts at: 1872 4066 1872 Size: 2194 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2333 3072 3329 4843 5744 6006 Size: 197 926 390 739 257 1514 901 262 Cuts at: 6006 820 Size: 1553 Fragments arranged by size: 1553 1514 926 901 739 390 262 257 197 SnaBI TAC'GTA Cuts at: 493 4036 4530 493 Size: 3543 494 2702 Fragments arranged by size: 3543 2702 494 SpeI A'CTAG_T Cuts at: 152 3365 152 Size: 3213 3526 SphI G_CATG'C Cuts at: 1132 4097 1132 Size: 2965 3774 SspI AAT'ATT Cuts at: 5 52 2149 4663 5 Size: 47 2097 2514 2081 Fragments arranged by size: 2514 2097 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6739 StyI C'CwwG_G Cuts at: 513 1065 2022 4178 513 Size: 552 957 2156 3074 Fragments arranged by size: 3074 2156 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4766 4783 4936 5121 5566 325 Size: 1255 3186 17 153 185 445 1498 Fragments arranged by size: 3186 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2444 4985 6697 Size: 274 80 33 51 1443 467 2541 1712 Cuts at: 6697 96 Size: 138 Fragments arranged by size: 2541 1712 1443 467 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6699 6731 6699 Size: 32 6707 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6739 XbaI T'CTAG_A Cuts at: 4075 4075 Size: 6739 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6739 XmaI C'CCGG_G Cuts at: 1870 4064 1870 Size: 2194 4545 XmnI GAAnn'nnTTC Cuts at: 4868 4868 Size: 6739 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4547 276 Size: 53 83 186 936 3013 2468 Fragments arranged by size: 3013 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI