Restriction map for
pFN21ASDA0887
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
AlwNI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BanII BbrPI BbvCI
BcgI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BseAI
BsgI BsiEI BsiWI BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
ScaI SfuI SgrAI SmaI SnaBI
SpeI SspI SwaI Tth111I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AcsI 1011 GAATTT [GA]^AATT[CT]
AcyI 6 GGCGCC G[GA]^CG[CT]C
Alw44I 581 GTGCAC G^TGCAC
ApaLI 581 GTGCAC G^TGCAC
ApoI 1011 GAATTT [GA]^AATT[CT]
Asp700I 1195 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 581 GTGCAC G[AT]GC[AT]^C
AvaI 1115 CTCGAG C^[CT]CG[GA]G
AvaII 1320 GGACC G^G[AT]CC
BalI 698 TGGCCA TGG^CCA
BanI 6 GGCGCC G^G[CT][GA]CC
BbeI 6 GGCGCC GGCGC^C
BbsI 457 GTCTTC GAAGAC(2/6)
BciVI 652 GGATAC GTATCC(6/5)
BclI 540 TGATCA T^GATCA
BcnI 381 CCGGG CC^[GC]GG
BglI 886 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 457 GTCTTC GAAGAC(2/6)
BsaHI 6 GGCGCC G[GA]^CG[CT]C
BsaI 660 GGTCTC GGTCTC(1/5)
BsaWI 308 TCCGGT [AT]^CCGG[AT]
BsiHKAI 581 GTGCAC G[AT]GC[AT]^C
BsmI 1042 GAATGC GAATGC(1/-1)
BsoBI 1115 CTCGAG C^[CT]CG[GA]G
Bsp1407I 138 TGTACA T^GTACA
BspMI 207 GCAGGT ACCTGC(4/8)
BsrGI 138 TGTACA T^GTACA
CfoI 7 GCGC GCG^C
DraII 1319 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 575 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 698 TGGCCA [CT]^GGCC[GA]
EcoO109I 1319 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 540 TGATCA T^GATCA
HaeII 6 GGCGCC [GA]GCGC^[CT]
HhaI 7 GCGC GCG^C
Hin1I 6 GGCGCC G[GA]^CG[CT]C
HinP1I 7 GCGC G^CGC
HphI 543 TCACC GGTGA(8/7)
KasI 6 GGCGCC G^GCGCC
MaeII 472 ACGT A^CGT
MaeIII 385 GTCAC ^GT[ACGT]AC
MluNI 698 TGGCCA TGG^CCA
MscI 698 TGGCCA TGG^CCA
MspA1I 218 CAGCTG C[AC]G^C[GT]G
NarI 6 GGCGCC GG^CGCC
NciI 381 CCGGG CC^[GC]GG
NlaIV 6 GGCGCC GG[ACGT]^[ACGT]CC
NspI 628 GCATGC [GA]CATG^[CT]
PaeR7I 1115 CTCGAG C^TCGAG
PflMI 99 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1319 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 218 CAGCTG CAG^CTG
SbfI 204 CCTGCAGG CCTGCA^GG
SexAI 815 ACCTGGT A^CC[AT]GGT
SfiI 885 GGCCTACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 6 GGCGCC GGC^GCC
SphI 628 GCATGC GCATG^C
Sse8387I 204 CCTGCAGG CCTGCA^GG
SspBI 138 TGTACA T^GTACA
Tsp45I 385 GTCAC ^GT[GC]AC
Van91I 99 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1115 CTCGAG C^TCGAG
XmnI 1195 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 10 CCTGAGG CC^T[ACGT]AGG
646 CCTGAGG
BmyI 446 GGGCAC G[AGT]GC[ACT]^C
581 GTGCAC
BsmBI 999 GAGACG CGTCTC(1/5)
1125 GAGACG
BsmFI 401 GGGAC GGGAC(10/14)
738 GGGAC
Bsp1286I 446 GGGCAC G[AGT]GC[ACT]^C
581 GTGCAC
BsrBI 16 GAGCGG CCGCTC(-3/-3)
940 GAGCGG
BsrI 70 ACTGG ACTGG(1/-1)
119 ACTGG
Bsu36I 10 CCTGAGG CC^T[ACGT]AGG
646 CCTGAGG
Cac8I 628 GCATGC GC[ACGT]^[ACGT]GC
1046 GCCTGC
Cfr13I 1259 GGCCC G^G[ACGT]CC
1320 GGACC
Eco81I 10 CCTGAGG CC^T[ACGT]AGG
646 CCTGAGG
EcoT14I 104 CCTTGG C^C[AT][AT]GG
262 CCAAGG
FauI 18 GCGGG CCCGC(4/6)
942 GCGGG
PleI 550 GACTC GAGTC(4/5)
1289 GACTC
PstI 52 CTGCAG CTGCA^G
205 CTGCAG
SapI 349 GCTCTTC GCTCTTC(1/4)
618 GCTCTTC
Sau96I 1259 GGCCC G^G[ACGT]CC
1320 GGACC
SmlI 249 CTCAAG C^T[CT][GA]AG
1115 CTCGAG
StuI 750 AGGCCT AGG^CCT
884 AGGCCT
StyI 104 CCTTGG C^C[AT][AT]GG
262 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BsaJI BseRI BsiYI BslI
BsmAI BssKI BstF5I BstNI BstYI
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeI MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII