(Circular) (Six-base) MAPSORT of: pFN21ASDA0887.seq Check: 5910 from: 1 to: 6064 pFN21ASDA0887 6064 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3407 1937 Size: 1470 4594 Acc65I G'GTAC_C Cuts at: 3385 3385 Size: 6064 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2631 2756 3255 4127 5511 5779 1632 Size: 999 125 499 872 1384 268 1917 Fragments arranged by size: 1917 1384 999 872 499 268 125 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 4963 5940 1203 Size: 3760 977 1327 Fragments arranged by size: 3760 1327 977 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 4793 4793 Size: 6064 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AlwNI CAG_nnn'CTG Cuts at: 3807 5379 3807 Size: 1572 4492 ApaLI G'TGCA_C Cuts at: 2604 4121 5277 2604 Size: 1517 1156 3391 Fragments arranged by size: 3391 1517 1156 ApoI r'AATT_y Cuts at: 1493 3034 3373 3599 3751 5860 1493 Size: 1541 339 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 339 226 152 AseI AT'TA_AT Cuts at: 160 1032 4618 160 Size: 872 3586 1606 Fragments arranged by size: 3586 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 3138 3389 1870 Size: 73 1195 251 4545 Fragments arranged by size: 4545 1195 251 73 BamHI G'GATC_C Cuts at: 3394 3761 3394 Size: 367 5697 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 3385 4840 618 Size: 325 217 627 242 1356 1455 1842 Fragments arranged by size: 1842 1455 1356 627 325 242 217 BanII G_rGCy'C Cuts at: 1391 3383 1391 Size: 1992 4072 BbeI G_GCGC'C Cuts at: 2033 2033 Size: 6064 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2473 928 Size: 971 574 4519 Fragments arranged by size: 4519 971 574 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4255 4289 1903 Size: 34 2318 34 3678 Fragments arranged by size: 3678 2318 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2669 3956 5166 2669 Size: 1287 1210 3567 Fragments arranged by size: 3567 1287 1210 BclI T'GATC_A Cuts at: 1548 2563 1548 Size: 1015 5049 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2915 4675 136 Size: 107 122 71 631 605 1243 1760 1525 Fragments arranged by size: 1760 1525 1243 631 605 122 107 71 BglII A'GATC_T Cuts at: 6059 6059 Size: 6064 BlpI GC'TnA_GC Cuts at: 3481 3481 Size: 6064 Bme1580I G_kGCm'C Cuts at: 1790 2473 2608 4125 5281 1790 Size: 683 135 1517 1156 2573 Fragments arranged by size: 2573 1517 1156 683 135 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3795 4753 442 Size: 416 53 83 399 24 2378 958 1753 Fragments arranged by size: 2378 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4724 1239 Size: 3485 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2257 4189 5054 5352 2257 Size: 1932 865 298 2969 Fragments arranged by size: 2969 1932 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2689 4727 882 Size: 617 1190 2038 2219 Fragments arranged by size: 2219 2038 1190 617 BsaAI yAC'GTr Cuts at: 493 1204 3361 3855 493 Size: 711 2157 494 2702 Fragments arranged by size: 2702 2157 711 494 BsaBI GATnn'nnATC Cuts at: 6058 6058 Size: 6064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 3871 4253 4888 Size: 53 83 186 936 497 1841 382 635 Cuts at: 4888 275 Size: 1451 Fragments arranged by size: 1841 1451 936 635 497 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2331 4497 4895 5169 5316 5727 2331 Size: 2166 398 274 147 411 2668 Fragments arranged by size: 2668 2166 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5839 Fragments arranged by size: 5839 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2975 3002 3005 3032 3290 1186 Size: 865 924 27 3 27 258 3960 Fragments arranged by size: 3960 924 865 258 27 27 3 BseYI C'CCAG_C Cuts at: 5267 5763 5267 Size: 496 5568 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5491 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2608 4125 4210 5281 2608 Size: 1517 85 1071 3391 Fragments arranged by size: 3391 1517 1071 85 BsmI GAATG_Cn' Cuts at: 3071 3575 3668 3071 Size: 504 93 5467 Fragments arranged by size: 5467 504 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3016 3142 3016 Size: 126 5938 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2473 2608 3383 4125 4210 5281 1391 Size: 399 683 135 775 742 85 1071 2174 Fragments arranged by size: 2174 1071 775 742 683 399 135 85 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 3953 5683 3953 Size: 1730 4334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2221 3419 844 Size: 1377 1198 3489 Fragments arranged by size: 3489 1377 1198 BsrBI CCG'CTC Cuts at: 1394 1823 2041 2965 3951 1394 Size: 429 218 924 986 3507 Fragments arranged by size: 3507 986 924 429 218 BsrDI GCAATG_nn' Cuts at: 65 1248 4559 4733 65 Size: 1183 3311 174 1396 Fragments arranged by size: 3311 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4708 4895 1700 Size: 110 2898 187 2869 Fragments arranged by size: 2898 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2161 96 Size: 2065 3999 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6064 BssSI C'ACGA_G Cuts at: 4124 5136 4124 Size: 1012 5052 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6064 BstBI TT'CG_AA Cuts at: 2007 4868 2007 Size: 2861 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6064 BstYI r'GATC_y Cuts at: 1972 2044 2086 2253 3394 3761 4146 4163 5615 Size: 72 42 167 1141 367 385 17 1452 Cuts at: 5615 5701 5713 6059 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1141 385 367 346 167 86 72 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2034 2670 2034 Size: 636 5428 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4555 Fragments arranged by size: 4555 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5325 Fragments arranged by size: 5325 683 56 BtsI GCAGTG_nn' Cuts at: 3580 4374 4394 3580 Size: 794 20 5250 Fragments arranged by size: 5250 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6064 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DraIII CAC_nnn'GTG Cuts at: 2603 2603 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2721 3511 4400 Size: 54 1425 50 275 18 891 790 889 Cuts at: 4400 6029 8 Size: 1629 43 Fragments arranged by size: 1629 1425 891 889 790 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 2448 2648 3994 1353 Size: 1026 69 200 1346 3423 Fragments arranged by size: 3423 1346 1026 200 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2995 4651 5025 5171 387 Size: 1064 138 1406 1656 374 146 1280 Fragments arranged by size: 1656 1406 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2631 2756 3255 4127 4724 5511 5779 Size: 393 999 125 499 872 597 787 268 Cuts at: 5779 1239 Size: 1524 Fragments arranged by size: 1524 999 872 787 597 499 393 268 125 EcoO109I rG'GnC_Cy Cuts at: 3343 3343 Size: 6064 EcoRI G'AATT_C Cuts at: 3373 3751 3373 Size: 378 5686 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3133 3133 Size: 6064 FspI TGC'GCA Cuts at: 4570 4570 Size: 6064 HaeII r_GCGC'y Cuts at: 1678 2033 5211 1678 Size: 355 3178 2531 Fragments arranged by size: 3178 2531 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2667 2699 4705 4737 4779 Size: 32 135 32 1095 32 2006 32 42 Cuts at: 4779 4811 1373 Size: 32 2626 Fragments arranged by size: 2626 2006 1095 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3408 3654 669 Size: 988 281 1470 246 3079 Fragments arranged by size: 3079 1470 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 3654 3654 Size: 6064 KasI G'GCGC_C Cuts at: 2029 2029 Size: 6064 KpnI G_GTAC'C Cuts at: 3389 3389 Size: 6064 MfeI C'AATT_G Cuts at: 3663 3663 Size: 6064 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3042 5178 5362 1305 Size: 1737 2136 184 2007 Fragments arranged by size: 2136 2007 1737 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2723 10 Size: 54 1425 343 891 3351 Fragments arranged by size: 3351 1425 891 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2559 2636 2729 4022 4381 4540 518 Size: 648 1393 77 93 1293 359 159 2042 Fragments arranged by size: 2042 1393 1293 648 359 159 93 77 MspA1I CmG'CkG Cuts at: 1606 2243 3480 4157 5305 5550 1606 Size: 637 1237 677 1148 245 2120 Fragments arranged by size: 2120 1237 1148 677 637 245 NarI GG'CG_CC Cuts at: 2030 2030 Size: 6064 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4555 Fragments arranged by size: 4555 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6064 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2655 3422 4967 1132 Size: 97 1426 767 1545 2229 Fragments arranged by size: 2229 1545 1426 767 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 4963 4963 Size: 6064 PflMI CCAn_nnn'nTGG Cuts at: 1108 2128 1108 Size: 1020 5044 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3343 3343 Size: 6064 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6064 PsiI TTA'TAA Cuts at: 3634 3634 Size: 6064 PstI C_TGCA'G Cuts at: 830 2079 2232 3416 830 Size: 1249 153 1184 3478 Fragments arranged by size: 3478 1249 1184 153 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 PvuII CAG'CTG Cuts at: 2243 2243 Size: 6064 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SapI GCTCTTCn'nnn_ Cuts at: 2379 2648 2379 Size: 269 5795 SbfI CC_TGCA'GG Cuts at: 2232 3416 2232 Size: 1184 4880 ScaI AGT'ACT Cuts at: 4312 6024 4312 Size: 1712 4352 SexAI A'CCwGG_T Cuts at: 2838 2838 Size: 6064 SfcI C'TryA_G Cuts at: 826 1045 2075 2228 2257 3299 3412 4547 5228 Size: 219 1030 153 29 1042 113 1135 681 Cuts at: 5228 5419 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1042 1030 681 219 191 153 113 29 SfiI GGCCn_nnn'nGGCC Cuts at: 2915 2915 Size: 6064 SfoI GGC'GCC Cuts at: 2031 2031 Size: 6064 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 3391 1872 Size: 1519 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2272 3138 4168 5069 5331 820 Size: 197 926 329 866 1030 901 262 1553 Fragments arranged by size: 1553 1030 926 901 866 329 262 197 SnaBI TAC'GTA Cuts at: 493 3361 3855 493 Size: 2868 494 2702 Fragments arranged by size: 2868 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 2655 3422 1132 Size: 1523 767 3774 Fragments arranged by size: 3774 1523 767 SspI AAT'ATT Cuts at: 5 52 3988 5 Size: 47 3936 2081 Fragments arranged by size: 3936 2081 47 StuI AGG'CCT Cuts at: 1517 2775 2909 1517 Size: 1258 134 4672 Fragments arranged by size: 4672 1258 134 StyI C'CwwG_G Cuts at: 513 1065 2022 2127 2285 3503 513 Size: 552 957 105 158 1218 3074 Fragments arranged by size: 3074 1218 957 552 158 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4091 4108 4261 4446 4891 325 Size: 1255 2511 17 153 185 445 1498 Fragments arranged by size: 2511 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2161 4310 6022 Size: 274 80 33 51 1443 184 2149 1712 Cuts at: 6022 96 Size: 138 Fragments arranged by size: 2149 1712 1443 274 184 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6024 6056 6024 Size: 32 6032 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6064 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XhoI C'TCGA_G Cuts at: 1943 3138 1943 Size: 1195 4869 XmaI C'CCGG_G Cuts at: 1870 3389 1870 Size: 1519 4545 XmnI GAAnn'nnTTC Cuts at: 3222 4193 3222 Size: 971 5093 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SrfI SwaI XcmI