Restriction map for
pFN21ASDA0886
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI Asp700I
Asp718I AspEI AspI AviII BamHI
BbrPI BbvCI BcgI BfrI BglI
BglII BlpI Bpu1102I BsaAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspMI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRV EcoT22I
FseI FspI HgaI HpaI KpnI
MluI NcoI NheI NotI NruI
NsiI NspV PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PvuI PvuII RcaI RsrII SbfI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 342 GTCGAC GT^[AC][GT]AC
AccIII 2136 TCCGGA T^CCGGA
AclI 2571 AACGTT AA^CGTT
AflIII 1387 ACATGT A^C[GA][CT]GT
AscI 223 GGCGCGCC GG^CGCGCC
AseI 2047 ATTAAT AT^TAAT
AsnI 2047 ATTAAT AT^TAAT
AvrII 955 CCTAGG C^CTAGG
BalI 2873 TGGCCA TGG^CCA
BciVI 2680 GTATCC GTATCC(6/5)
BlnI 955 CCTAGG C^CTAGG
BsaWI 2136 TCCGGA [AT]^CCGG[AT]
BseAI 2136 TCCGGA T^CCGGA
BsgI 3300 CTGCAC GTGCAG(16/14)
BspEI 2136 TCCGGA T^CCGGA
BspLU11I 1387 ACATGT A^CATGT
BssHII 224 GCGCGC G^CGCGC
BstAPI 928 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1452 AGATCC [GA]^GATC[CT]
DsaI 191 CCGCGG C^C[GA][CT]GG
EcoRI 1860 GAATTC G^AATTC
KspI 191 CCGCGG CCGC^GG
MfeI 2591 CAATTG C^AATTG
MflI 1452 AGATCC [GA]^GATC[CT]
MluNI 2873 TGGCCA TGG^CCA
MroI 2136 TCCGGA T^CCGGA
MscI 2873 TGGCCA TGG^CCA
MunI 2591 CAATTG C^AATTG
NdeI 1973 CATATG CA^TATG
PacI 2101 TTAATTAA TTAAT^TAA
PshBI 2047 ATTAAT AT^TAAT
Psp1406I 2571 AACGTT AA^CGTT
SacI 2500 GAGCTC GAGCT^C
SacII 191 CCGCGG CCGC^GG
SalI 342 GTCGAC G^TCGAC
ScaI 2506 AGTACT AGT^ACT
SmaI 269 CCCGGG CCC^GGG
SspI 2044 AATATT AAT^ATT
StuI 1644 AGGCCT AGG^CCT
XhoII 1452 AGATCC [GA]^GATC[CT]
XmaCI 269 CCCGGG C^CCGGG
XmaI 269 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcsI 1860 GAATTC [GA]^AATT[CT]
2480 AAATTC
AcyI 264 GGCGCC G[GA]^CG[CT]C
3320 GGCGCC
AocI 400 CCTGAGG CC^T[ACGT]AGG
2377 CCTGAGG
ApoI 1860 GAATTC [GA]^AATT[CT]
2480 AAATTC
BbeI 264 GGCGCC GGCGC^C
3320 GGCGCC
BclI 2187 TGATCA T^GATCA
2621 TGATCA
BsaBI 2969 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3487 GATCATTATC
BsaHI 264 GGCGCC G[GA]^CG[CT]C
3320 GGCGCC
BsmFI 273 GGGAC GGGAC(10/14)
323 GGGAC
BsrBI 287 CCGCTC CCGCTC(-3/-3)
307 GAGCGG
Bsu36I 400 CCTGAGG CC^T[ACGT]AGG
2377 CCTGAGG
DraII 273 GGGACCC [GA]G^G[ACGT]CC[CT]
323 GGGACCC
Eco81I 400 CCTGAGG CC^T[ACGT]AGG
2377 CCTGAGG
EcoNI 1441 CCTTTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2377 CCTGAGGGAGG
EcoO109I 273 GGGACCC [GA]G^G[ACGT]CC[CT]
323 GGGACCC
FbaI 2187 TGATCA T^GATCA
2621 TGATCA
HaeII 264 GGCGCC [GA]GCGC^[CT]
3320 GGCGCC
Hin1I 264 GGCGCC G[GA]^CG[CT]C
3320 GGCGCC
KasI 264 GGCGCC G^GCGCC
3320 GGCGCC
MaeII 2264 ACGT A^CGT
2572 ACGT
MamI 2969 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3487 GATCATTATC
MslI 1839 CATTGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2686 CACAAAAGTG
MspA1I 191 CCGCGG C[AC]G^C[GT]G
364 CCGCTG
NarI 264 GGCGCC GG^CGCC
3320 GGCGCC
PpuMI 273 GGGACCC [GA]G^G[AT]CC[CT]
323 GGGACCC
SapI 571 GCTCTTC GCTCTTC(1/4)
2056 GCTCTTC
SfoI 264 GGCGCC GGC^GCC
3320 GGCGCC
SgrAI 219 CGCCGGCG C[GA]^CCGG[CT]G
260 CGCCGGCG
SmlI 1285 CTTGAG C^T[CT][GA]AG
2879 CTCAAG
TaqI 343 TCGA T^CGA
1235 TCGA
TthHB8I 343 TCGA T^CGA
1235 TCGA
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EaeI EagI EarI EclXI
Eco52I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI