(Circular) (Six-base) MAPSORT of: pFN21ASDA0886.seq Check: 1454 from: 1 to: 8305 pFN21ASDA0886 8305 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6115 278 Size: 53 83 186 936 4579 2468 Fragments arranged by size: 4579 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2366 5648 1937 Size: 429 3282 4594 Fragments arranged by size: 4594 3282 429 Acc65I G'GTAC_C Cuts at: 5626 5626 Size: 8305 AclI AA'CG_TT Cuts at: 1563 4595 6432 6805 1563 Size: 3032 1837 373 3063 Fragments arranged by size: 3063 3032 1837 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2826 3090 3468 3684 3933 5016 5280 5399 Size: 1194 264 378 216 249 1083 264 119 Cuts at: 5399 5452 6368 7752 8020 1632 Size: 53 916 1384 268 1917 Fragments arranged by size: 1917 1384 1194 1083 916 378 268 264 264 249 216 119 53 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8108 AflIII A'CryG_T Cuts at: 1203 3410 7204 8181 1203 Size: 2207 3794 977 1327 Fragments arranged by size: 3794 2207 1327 977 AgeI A'CCGG_T Cuts at: 7136 7136 Size: 8305 AhdI GACnn_n'nnGTC Cuts at: 7034 7034 Size: 8305 AleI CACnn'nnGTG Cuts at: 1166 4713 1166 Size: 3547 4758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3826 4781 1184 Size: 2642 955 4708 Fragments arranged by size: 4708 2642 955 AlwNI CAG_nnn'CTG Cuts at: 2957 3437 4208 6048 7620 2957 Size: 480 771 1840 1572 3642 Fragments arranged by size: 3642 1840 1572 771 480 ApaLI G'TGCA_C Cuts at: 6362 7518 6362 Size: 1156 7149 ApoI r'AATT_y Cuts at: 1493 3883 4503 5614 5840 5992 8101 1493 Size: 2390 620 1111 226 152 2109 1697 Fragments arranged by size: 2390 2109 1697 1111 620 226 152 AscI GG'CGCG_CC Cuts at: 2247 2247 Size: 8305 AseI AT'TA_AT Cuts at: 160 1032 4071 6859 160 Size: 872 3039 2788 1606 Fragments arranged by size: 3039 2788 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8305 AvaI C'yCGr_G Cuts at: 1870 1943 2114 2292 2350 2566 5630 1870 Size: 73 171 178 58 216 3064 4545 Fragments arranged by size: 4545 3064 216 178 171 73 58 AvrII C'CTAG_G Cuts at: 2978 2978 Size: 8305 BamHI G'GATC_C Cuts at: 5635 6002 5635 Size: 367 7938 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2287 5087 5343 5417 5626 Size: 325 217 627 500 2800 256 74 209 Cuts at: 5626 7081 618 Size: 1455 1842 Fragments arranged by size: 2800 1842 1455 627 500 325 256 217 209 74 BanII G_rGCy'C Cuts at: 1391 2115 2357 2480 2493 2564 2573 4527 5624 Size: 724 242 123 13 71 9 1954 1097 Cuts at: 5624 1391 Size: 4072 Fragments arranged by size: 4072 1954 1097 724 242 123 71 13 9 BbeI G_GCGC'C Cuts at: 2291 5347 2291 Size: 3056 5249 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2034 2169 3096 5075 928 Size: 971 135 135 927 1979 4158 Fragments arranged by size: 4158 1979 971 927 135 135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6496 6530 1903 Size: 34 4559 34 3678 Fragments arranged by size: 4559 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4714 6197 7407 4714 Size: 1483 1210 5612 Fragments arranged by size: 5612 1483 1210 BclI T'GATC_A Cuts at: 1548 4210 4644 1548 Size: 2662 434 5209 Fragments arranged by size: 5209 2662 434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6916 136 Size: 107 122 71 631 605 5244 1525 Fragments arranged by size: 5244 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8300 8300 Size: 8305 BlpI GC'TnA_GC Cuts at: 5722 5722 Size: 8305 Bme1580I G_kGCm'C Cuts at: 1790 2229 2376 6366 7522 1790 Size: 439 147 3990 1156 2573 Fragments arranged by size: 3990 2573 1156 439 147 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8305 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2216 3360 6036 Size: 416 53 83 399 24 799 1144 2676 Cuts at: 6036 6994 442 Size: 958 1753 Fragments arranged by size: 2676 1753 1144 958 799 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8305 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2198 2207 3267 3660 4863 5074 5094 Size: 950 9 9 1060 393 1203 211 20 Cuts at: 5094 6965 1239 Size: 1871 2579 Fragments arranged by size: 2579 1871 1203 1060 950 393 211 20 9 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3329 4887 6430 7295 7593 3329 Size: 1558 1543 865 298 4041 Fragments arranged by size: 4041 1558 1543 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2056 2576 2854 6968 882 Size: 617 557 520 278 4114 2219 Fragments arranged by size: 4114 2219 617 557 520 278 BsaAI yAC'GTr Cuts at: 493 1204 5602 6096 493 Size: 711 4398 494 2702 Fragments arranged by size: 4398 2702 711 494 BsaBI GATnn'nnATC Cuts at: 4996 5514 8299 4996 Size: 518 2785 5002 Fragments arranged by size: 5002 2785 518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2288 5344 6112 6494 Size: 53 83 186 936 755 3056 768 382 Cuts at: 6494 7129 275 Size: 635 1451 Fragments arranged by size: 3056 1451 936 768 755 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4159 6738 7136 7410 7557 7968 4159 Size: 2579 398 274 147 411 4496 Fragments arranged by size: 4496 2579 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3400 3430 4081 4111 1178 Size: 30 165 30 1997 30 651 30 5372 Fragments arranged by size: 5372 1997 651 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2034 2138 2150 2153 2162 2186 2448 2554 Size: 848 104 12 3 9 24 262 106 Cuts at: 2554 2617 2730 4535 5291 1186 Size: 63 113 1805 756 4200 Fragments arranged by size: 4200 1805 848 756 262 113 106 104 63 24 12 9 3 BseYI C'CCAG_C Cuts at: 3427 3923 4742 7508 8004 3427 Size: 496 819 2766 496 3728 Fragments arranged by size: 3728 2766 819 496 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5308 1163 Size: 573 3572 4160 Fragments arranged by size: 4160 3572 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2078 2310 2370 2544 5755 6516 Size: 275 186 77 232 60 174 3211 761 Cuts at: 6516 7544 1540 Size: 1028 2301 Fragments arranged by size: 3211 2301 1028 761 275 232 186 174 77 60 BsiHKAI G_wGCw'C Cuts at: 3418 4527 4869 6366 6451 7522 3418 Size: 1109 342 1497 85 1071 4201 Fragments arranged by size: 4201 1497 1109 1071 342 85 BsmI GAATG_Cn' Cuts at: 5816 5909 5816 Size: 93 8212 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2229 2357 2376 2480 2493 2564 Size: 399 325 114 128 19 104 13 71 Cuts at: 2564 2573 3418 4527 4869 5624 6366 6451 7522 Size: 9 845 1109 342 755 742 85 1071 Cuts at: 7522 1391 Size: 2174 Fragments arranged by size: 2174 1109 1071 845 755 742 399 342 325 128 114 104 85 71 19 13 9 BspEI T'CCGG_A Cuts at: 4159 7968 4159 Size: 3809 4496 BspHI T'CATG_A Cuts at: 6194 7924 6194 Size: 1730 6575 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5660 844 Size: 4816 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2312 2332 6192 1394 Size: 429 489 20 3860 3507 Fragments arranged by size: 3860 3507 489 429 20 BsrDI GCAATG_nn' Cuts at: 65 1248 3444 4748 5148 6800 6974 65 Size: 1183 2196 1304 400 1652 174 1396 Fragments arranged by size: 2196 1652 1396 1304 1183 400 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2243 2284 2305 6949 7136 1700 Size: 110 433 41 21 4644 187 2869 Fragments arranged by size: 4644 2869 433 187 110 41 21 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8305 BssHII G'CGCG_C Cuts at: 1925 2247 1925 Size: 322 7983 BssSI C'ACGA_G Cuts at: 6365 7377 6365 Size: 1012 7293 BstAPI GCAn_nnn'nTGC Cuts at: 794 2957 794 Size: 2163 6142 BstBI TT'CG_AA Cuts at: 2007 7109 2007 Size: 5102 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8305 BstYI r'GATC_y Cuts at: 1972 3475 5635 6002 6387 6404 7856 7942 7954 Size: 1503 2160 367 385 17 1452 86 12 Cuts at: 7954 8300 1972 Size: 346 1977 Fragments arranged by size: 2160 1977 1503 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2424 4401 2424 Size: 1977 6328 BtgI C'CryG_G Cuts at: 513 1065 2022 2214 513 Size: 552 957 192 6604 Fragments arranged by size: 6604 957 552 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7566 Fragments arranged by size: 7566 683 56 BtsI GCAGTG_nn' Cuts at: 3153 5394 5821 6615 6635 3153 Size: 2241 427 794 20 4823 Fragments arranged by size: 4823 2241 794 427 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8305 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8270 DraI TTT'AAA Cuts at: 5609 5609 Size: 8305 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7312 809 Size: 507 5996 1802 Fragments arranged by size: 5996 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2075 2307 2466 Size: 54 1425 50 275 18 245 232 159 Cuts at: 2466 2541 4896 5752 6641 8270 8 Size: 75 2355 856 889 1629 43 Fragments arranged by size: 2355 1629 1425 889 856 275 245 232 159 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2075 2307 2541 5752 1537 Size: 275 263 232 234 3211 4090 Fragments arranged by size: 4090 3211 275 263 234 232 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2139 2601 2730 3009 3819 3996 4086 Size: 774 12 462 129 279 810 177 90 Cuts at: 4086 4803 5354 5471 6235 1353 Size: 717 551 117 764 3423 Fragments arranged by size: 3423 810 774 764 717 551 462 279 177 129 117 90 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6892 7266 7412 387 Size: 1064 138 5303 374 146 1280 Fragments arranged by size: 5303 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4525 4525 Size: 8305 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2198 2207 2826 3090 3267 3468 Size: 393 557 9 9 619 264 177 201 Cuts at: 3468 3660 3684 3933 4863 5016 5074 5094 5280 Size: 192 24 249 930 153 58 20 186 Cuts at: 5280 5399 5452 6368 6965 7752 8020 1239 Size: 119 53 916 597 787 268 1524 Fragments arranged by size: 1524 930 916 787 619 597 557 393 268 264 249 201 192 186 177 153 119 58 53 24 20 9 9 EcoNI CCTnn'n_nnAGG Cuts at: 3468 4404 3468 Size: 936 7369 EcoO109I rG'GnC_Cy Cuts at: 2297 2347 2297 Size: 50 8255 EcoRI G'AATT_C Cuts at: 3883 5614 5992 3883 Size: 1731 378 6196 Fragments arranged by size: 6196 1731 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2831 4885 2831 Size: 2054 6251 FspI TGC'GCA Cuts at: 6811 6811 Size: 8305 HaeII r_GCGC'y Cuts at: 1678 2291 5347 7452 1678 Size: 613 3056 2105 2531 Fragments arranged by size: 3056 2531 2105 613 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2681 2713 3240 3272 3390 Size: 32 135 32 1109 32 527 32 118 Cuts at: 3390 3422 4976 5008 5437 5469 6946 6978 7020 Size: 32 1554 32 429 32 1477 32 42 Cuts at: 7020 7052 1373 Size: 32 2626 Fragments arranged by size: 2626 1554 1477 1109 527 429 135 118 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2367 2790 4258 5041 5649 5895 Size: 988 281 429 423 1468 783 608 246 Cuts at: 5895 669 Size: 3079 Fragments arranged by size: 3079 1468 988 783 608 429 423 281 246 HindIII A'AGCT_T Cuts at: 748 2835 3942 4139 748 Size: 2087 1107 197 4914 Fragments arranged by size: 4914 2087 1107 197 HpaI GTT'AAC Cuts at: 5895 5895 Size: 8305 KasI G'GCGC_C Cuts at: 2287 5343 2287 Size: 3056 5249 KpnI G_GTAC'C Cuts at: 5630 5630 Size: 8305 MfeI C'AATT_G Cuts at: 4614 5904 4614 Size: 1290 7015 MluI A'CGCG_T Cuts at: 8181 8181 Size: 8305 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7419 7603 1305 Size: 6114 184 2007 Fragments arranged by size: 6114 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4898 10 Size: 54 1425 343 3066 3417 Fragments arranged by size: 3417 3066 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3866 4713 6263 6622 6781 518 Size: 648 2700 847 1550 359 159 2042 Fragments arranged by size: 2700 2042 1550 847 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2216 2389 5721 6398 7546 7791 1606 Size: 610 173 3332 677 1148 245 2120 Fragments arranged by size: 3332 2120 1148 677 610 245 173 NaeI GCC'GGC Cuts at: 2245 2286 2307 2245 Size: 41 21 8243 Fragments arranged by size: 8243 41 21 NarI GG'CG_CC Cuts at: 2288 5344 2288 Size: 3056 5249 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6796 Fragments arranged by size: 6796 957 552 NdeI CA'TA_TG Cuts at: 387 3997 387 Size: 3610 4695 NgoMIV G'CCGG_C Cuts at: 2243 2284 2305 2243 Size: 41 21 8243 Fragments arranged by size: 8243 41 21 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8305 NotI GC'GGCC_GC Cuts at: 5752 5752 Size: 8305 NspI r_CATG'y Cuts at: 1132 1229 3414 3661 3699 4119 5663 7208 1132 Size: 97 2185 247 38 420 1544 1545 2229 Fragments arranged by size: 2229 2185 1545 1544 420 247 97 38 PacI TTA_AT'TAA Cuts at: 4128 4128 Size: 8305 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8305 PciI A'CATG_T Cuts at: 3410 7204 3410 Size: 3794 4511 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8305 PmeI GTTT'AAAC Cuts at: 5609 5609 Size: 8305 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6374 6406 7887 7919 6374 Size: 32 1481 32 6760 Fragments arranged by size: 6760 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2297 2347 2297 Size: 50 8255 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8305 PsiI TTA'TAA Cuts at: 5875 5875 Size: 8305 PstI C_TGCA'G Cuts at: 830 2635 3802 3892 4537 5657 830 Size: 1805 1167 90 645 1120 3478 Fragments arranged by size: 3478 1805 1167 1120 645 90 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8305 SacI G_AGCT'C Cuts at: 4527 4527 Size: 8305 SacII CC_GC'GG Cuts at: 2217 2217 Size: 8305 SalI G'TCGA_C Cuts at: 1936 2365 5647 1936 Size: 429 3282 4594 Fragments arranged by size: 4594 3282 429 SanDI GG'GwC_CC Cuts at: 2297 2347 2297 Size: 50 8255 SapI GCTCTTCn'nnn_ Cuts at: 2601 4086 2601 Size: 1485 6820 SbfI CC_TGCA'GG Cuts at: 5657 5657 Size: 8305 ScaI AGT'ACT Cuts at: 4531 6553 8265 4531 Size: 2022 1712 4571 Fragments arranged by size: 4571 2022 1712 SfcI C'TryA_G Cuts at: 826 1045 2631 3063 3798 3888 4533 5653 6788 Size: 219 1586 432 735 90 645 1120 1135 Cuts at: 6788 7469 7660 826 Size: 681 191 1471 Fragments arranged by size: 1586 1471 1135 1120 735 681 645 432 219 191 90 SfoI GGC'GCC Cuts at: 2289 5345 2289 Size: 3056 5249 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8305 SgrAI Cr'CCGG_yG Cuts at: 1700 2243 2284 1700 Size: 543 41 7721 Fragments arranged by size: 7721 543 41 SmaI CCC'GGG Cuts at: 1872 2294 5632 1872 Size: 422 3338 4545 Fragments arranged by size: 4545 3338 422 SmlI C'TyrA_G Cuts at: 820 1017 1943 3308 4902 6409 7310 7572 820 Size: 197 926 1365 1594 1507 901 262 1553 Fragments arranged by size: 1594 1553 1507 1365 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5602 6096 493 Size: 5109 494 2702 Fragments arranged by size: 5109 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8305 SphI G_CATG'C Cuts at: 1132 5663 1132 Size: 4531 3774 SspI AAT'ATT Cuts at: 5 52 4069 6229 5 Size: 47 4017 2160 2081 Fragments arranged by size: 4017 2160 2081 47 StuI AGG'CCT Cuts at: 1517 3669 1517 Size: 2152 6153 StyI C'CwwG_G Cuts at: 513 1065 2022 2776 2978 5154 5744 513 Size: 552 957 754 202 2176 590 3074 Fragments arranged by size: 3074 2176 957 754 590 552 202 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3512 5250 6332 6349 6502 6687 7132 Size: 1255 1932 1738 1082 17 153 185 445 Cuts at: 7132 325 Size: 1498 Fragments arranged by size: 1932 1738 1498 1255 1082 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3685 4529 5049 Size: 274 80 33 51 1443 1708 844 520 Cuts at: 5049 5292 6551 8263 96 Size: 243 1259 1712 138 Fragments arranged by size: 1712 1708 1443 1259 844 520 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3400 3432 8265 8297 3400 Size: 32 4833 32 3408 Fragments arranged by size: 4833 3408 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8305 XbaI T'CTAG_A Cuts at: 5641 5641 Size: 8305 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2029 Size: 8305 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8305 XmaI C'CCGG_G Cuts at: 1870 2292 5630 1870 Size: 422 3338 4545 Fragments arranged by size: 4545 3338 422 XmnI GAAnn'nnTTC Cuts at: 6434 6434 Size: 8305 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6113 276 Size: 53 83 186 936 4579 2468 Fragments arranged by size: 4579 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SexAI SfiI SrfI SwaI