Restriction map for
pFN21ASDA0886
(Linear) (Six-base) MAPSORT of: KIAA0886.seq Check: 460 from: 1 to: 3576
KIAA0886.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 343 3576
Size: 343 3233
AclI AA'CG_TT
Cuts at: 0 2572 3576
Size: 2572 1004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1067 1445 1661 1910 2993 3257 3376
Size: 803 264 378 216 249 1083 264 119
Cuts at: 3376 3429 3576
Size: 53 147
Fragments arranged by size:
1083 803 378 264 264 249 216 147
119 53
AflIII A'CryG_T
Cuts at: 0 1387 3576
Size: 1387 2189
AleI CACnn'nnGTG
Cuts at: 0 2690 3576
Size: 2690 886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1803 2758 3576
Size: 1803 955 818
Fragments arranged by size:
1803 955 818
AlwNI CAG_nnn'CTG
Cuts at: 0 934 1414 2185 3576
Size: 934 480 771 1391
Fragments arranged by size:
1391 934 771 480
ApoI r'AATT_y
Cuts at: 0 1860 2480 3576
Size: 1860 620 1096
Fragments arranged by size:
1860 1096 620
AscI GG'CGCG_CC
Cuts at: 0 224 3576
Size: 224 3352
AseI AT'TA_AT
Cuts at: 0 2048 3576
Size: 2048 1528
AvaI C'yCGr_G
Cuts at: 0 91 269 327 543 3576
Size: 91 178 58 216 3033
Fragments arranged by size:
3033 216 178 91 58
AvrII C'CTAG_G
Cuts at: 0 955 3576
Size: 955 2621
BanI G'GyrC_C
Cuts at: 0 264 3064 3320 3394 3576
Size: 264 2800 256 74 182
Fragments arranged by size:
2800 264 256 182 74
BanII G_rGCy'C
Cuts at: 0 92 334 457 470 541 550 2504 3576
Size: 92 242 123 13 71 9 1954 1072
Fragments arranged by size:
1954 1072 242 123 92 71 13 9
BbeI G_GCGC'C
Cuts at: 0 268 3324 3576
Size: 268 3056 252
Fragments arranged by size:
3056 268 252
BbsI GAAGACnn'nnnn_
Cuts at: 0 11 146 1073 3052 3576
Size: 11 135 927 1979 524
Fragments arranged by size:
1979 927 524 135 11
BciVI GTATCCnnnnn_n'
Cuts at: 0 2691 3576
Size: 2691 885
BclI T'GATC_A
Cuts at: 0 2187 2621 3576
Size: 2187 434 955
Fragments arranged by size:
2187 955 434
Bme1580I G_kGCm'C
Cuts at: 0 206 353 3576
Size: 206 147 3223
Fragments arranged by size:
3223 206 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 193 1337 3576
Size: 193 1144 2239
Fragments arranged by size:
2239 1144 193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 175 184 1244 1637 2840 3051 3071
Size: 166 9 9 1060 393 1203 211 20
Cuts at: 3071 3576
Size: 505
Fragments arranged by size:
1203 1060 505 393 211 166 20 9
9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1306 2864 3576
Size: 1306 1558 712
Fragments arranged by size:
1558 1306 712
BsaI GGTCTCn'nnnn_
Cuts at: 0 33 553 831 3576
Size: 33 520 278 2745
Fragments arranged by size:
2745 520 278 33
BsaBI GATnn'nnATC
Cuts at: 0 2973 3491 3576
Size: 2973 518 85
Fragments arranged by size:
2973 518 85
BsaHI Gr'CG_yC
Cuts at: 0 265 3321 3576
Size: 265 3056 255
Fragments arranged by size:
3056 265 255
BsaWI w'CCGG_w
Cuts at: 0 2136 3576
Size: 2136 1440
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1377 1407 2058 2088 3576
Size: 1377 30 651 30 1488
Fragments arranged by size:
1488 1377 651 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 11 115 127 130 139 163 425 531
Size: 11 104 12 3 9 24 262 106
Cuts at: 531 594 707 2512 3268 3576
Size: 63 113 1805 756 308
Fragments arranged by size:
1805 756 308 262 113 106 104 63
24 12 11 9 3
BseYI C'CCAG_C
Cuts at: 0 1404 1900 2719 3576
Size: 1404 496 819 857
Fragments arranged by size:
1404 857 819 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3285 3576
Size: 3285 291
BsiEI CG_ry'CG
Cuts at: 0 55 287 347 521 3576
Size: 55 232 60 174 3055
Fragments arranged by size:
3055 232 174 60 55
BsiHKAI G_wGCw'C
Cuts at: 0 1395 2504 2846 3576
Size: 1395 1109 342 730
Fragments arranged by size:
1395 1109 730 342
Bsp1286I G_dGCh'C
Cuts at: 0 92 206 334 353 457 470 541 550
Size: 92 114 128 19 104 13 71 9
Cuts at: 550 1395 2504 2846 3576
Size: 845 1109 342 730
Fragments arranged by size:
1109 845 730 342 128 114 104 92
71 19 13 9
BspEI T'CCGG_A
Cuts at: 0 2136 3576
Size: 2136 1440
BsrBI CCG'CTC
Cuts at: 0 289 309 3576
Size: 289 20 3267
Fragments arranged by size:
3267 289 20
BsrDI GCAATG_nn'
Cuts at: 0 1421 2725 3125 3576
Size: 1421 1304 400 451
Fragments arranged by size:
1421 1304 451 400
BsrFI r'CCGG_y
Cuts at: 0 220 261 282 3576
Size: 220 41 21 3294
Fragments arranged by size:
3294 220 41 21
BssHII G'CGCG_C
Cuts at: 0 224 3576
Size: 224 3352
BstAPI GCAn_nnn'nTGC
Cuts at: 0 934 3576
Size: 934 2642
BstYI r'GATC_y
Cuts at: 0 1452 3576
Size: 1452 2124
Bsu36I CC'TnA_GG
Cuts at: 0 401 2378 3576
Size: 401 1977 1198
Fragments arranged by size:
1977 1198 401
BtgI C'CryG_G
Cuts at: 0 191 3576
Size: 191 3385
BtsI GCAGTG_nn'
Cuts at: 0 1130 3371 3576
Size: 1130 2241 205
Fragments arranged by size:
2241 1130 205
EaeI y'GGCC_r
Cuts at: 0 52 284 443 518 2873 3576
Size: 52 232 159 75 2355 703
Fragments arranged by size:
2355 703 232 159 75 52
EagI C'GGCC_G
Cuts at: 0 52 284 518 3576
Size: 52 232 234 3058
Fragments arranged by size:
3058 234 232 52
EarI CTCTTCn'nnn_
Cuts at: 0 104 116 578 707 986 1796 1973 2063
Size: 104 12 462 129 279 810 177 90
Cuts at: 2063 2780 3331 3448 3576
Size: 717 551 117 128
Fragments arranged by size:
810 717 551 462 279 177 129 128
117 104 90 12
EcoICRI GAG'CTC
Cuts at: 0 2502 3576
Size: 2502 1074
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 175 184 803 1067 1244 1445 1637
Size: 166 9 9 619 264 177 201 192
Cuts at: 1637 1661 1910 2840 2993 3051 3071 3257 3376
Size: 24 249 930 153 58 20 186 119
Cuts at: 3376 3429 3576
Size: 53 147
Fragments arranged by size:
930 619 264 249 201 192 186 177
166 153 147 119 58 53 24 20
9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1445 2381 3576
Size: 1445 936 1195
Fragments arranged by size:
1445 1195 936
EcoO109I rG'GnC_Cy
Cuts at: 0 274 324 3576
Size: 274 50 3252
Fragments arranged by size:
3252 274 50
EcoRI G'AATT_C
Cuts at: 0 1860 3576
Size: 1860 1716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 808 2862 3576
Size: 808 2054 714
Fragments arranged by size:
2054 808 714
HaeII r_GCGC'y
Cuts at: 0 268 3324 3576
Size: 268 3056 252
Fragments arranged by size:
3056 268 252
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 1217 1249 1367 1399 2953 2985
Size: 658 32 527 32 118 32 1554 32
Cuts at: 2985 3414 3446 3576
Size: 429 32 130
Fragments arranged by size:
1554 658 527 429 130 118 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 344 767 2235 3018 3576
Size: 344 423 1468 783 558
Fragments arranged by size:
1468 783 558 423 344
HindIII A'AGCT_T
Cuts at: 0 812 1919 2116 3576
Size: 812 1107 197 1460
Fragments arranged by size:
1460 1107 812 197
KasI G'GCGC_C
Cuts at: 0 264 3320 3576
Size: 264 3056 256
Fragments arranged by size:
3056 264 256
MfeI C'AATT_G
Cuts at: 0 2591 3576
Size: 2591 985
MscI TGG'CCA
Cuts at: 0 2875 3576
Size: 2875 701
MslI CAynn'nnrTG
Cuts at: 0 1843 2690 3576
Size: 1843 847 886
Fragments arranged by size:
1843 886 847
MspA1I CmG'CkG
Cuts at: 0 193 366 3576
Size: 193 173 3210
Fragments arranged by size:
3210 193 173
NaeI GCC'GGC
Cuts at: 0 222 263 284 3576
Size: 222 41 21 3292
Fragments arranged by size:
3292 222 41 21
NarI GG'CG_CC
Cuts at: 0 265 3321 3576
Size: 265 3056 255
Fragments arranged by size:
3056 265 255
NdeI CA'TA_TG
Cuts at: 0 1974 3576
Size: 1974 1602
NgoMIV G'CCGG_C
Cuts at: 0 220 261 282 3576
Size: 220 41 21 3294
Fragments arranged by size:
3294 220 41 21
NspI r_CATG'y
Cuts at: 0 1391 1638 1676 2096 3576
Size: 1391 247 38 420 1480
Fragments arranged by size:
1480 1391 420 247 38
PacI TTA_AT'TAA
Cuts at: 0 2105 3576
Size: 2105 1471
PciI A'CATG_T
Cuts at: 0 1387 3576
Size: 1387 2189
PpuMI rG'GwC_Cy
Cuts at: 0 274 324 3576
Size: 274 50 3252
Fragments arranged by size:
3252 274 50
PstI C_TGCA'G
Cuts at: 0 612 1779 1869 2514 3576
Size: 612 1167 90 645 1062
Fragments arranged by size:
1167 1062 645 612 90
SacI G_AGCT'C
Cuts at: 0 2504 3576
Size: 2504 1072
SacII CC_GC'GG
Cuts at: 0 194 3576
Size: 194 3382
SalI G'TCGA_C
Cuts at: 0 342 3576
Size: 342 3234
SanDI GG'GwC_CC
Cuts at: 0 274 324 3576
Size: 274 50 3252
Fragments arranged by size:
3252 274 50
SapI GCTCTTCn'nnn_
Cuts at: 0 578 2063 3576
Size: 578 1485 1513
Fragments arranged by size:
1513 1485 578
ScaI AGT'ACT
Cuts at: 0 2508 3576
Size: 2508 1068
SfcI C'TryA_G
Cuts at: 0 608 1040 1775 1865 2510 3576
Size: 608 432 735 90 645 1066
Fragments arranged by size:
1066 735 645 608 432 90
SfoI GGC'GCC
Cuts at: 0 266 3322 3576
Size: 266 3056 254
Fragments arranged by size:
3056 266 254
SgrAI Cr'CCGG_yG
Cuts at: 0 220 261 3576
Size: 220 41 3315
Fragments arranged by size:
3315 220 41
SmaI CCC'GGG
Cuts at: 0 271 3576
Size: 271 3305
SmlI C'TyrA_G
Cuts at: 0 1285 2879 3576
Size: 1285 1594 697
Fragments arranged by size:
1594 1285 697
SspI AAT'ATT
Cuts at: 0 2046 3576
Size: 2046 1530
StuI AGG'CCT
Cuts at: 0 1646 3576
Size: 1646 1930
StyI C'CwwG_G
Cuts at: 0 753 955 3131 3576
Size: 753 202 2176 445
Fragments arranged by size:
2176 753 445 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1489 3227 3576
Size: 1489 1738 349
Fragments arranged by size:
1738 1489 349
TatI w'GTAC_w
Cuts at: 0 1662 2506 3026 3269 3576
Size: 1662 844 520 243 307
Fragments arranged by size:
1662 844 520 307 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1377 1409 3576
Size: 1377 32 2167
Fragments arranged by size:
2167 1377 32
XmaI C'CCGG_G
Cuts at: 0 269 3576
Size: 269 3307
Enzymes that do cut:
AccI AclI AcuI AflIII AleI AlfI AlwNI ApoI
AscI AseI AvaI AvrII BanI BanII BbeI BbsI
BciVI BclI Bme1580I BmrI BpmI BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII BstAPI BstYI
Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
KasI MfeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PacI PciI PpuMI PstI SacI SacII
SalI SanDI SapI ScaI SfcI SfoI SgrAI SmaI
SmlI SspI StuI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AloI
ApaI ApaLI AsiSI BaeI BamHI BbvCI BcgI BfrBI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaAI
BsiWI BsmI BsmBI BspHI BspMI BsrGI BssSI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoRV FseI FspI FspAI HpaI KpnI
MluI MmeI NcoI NheI NotI NruI NsiI PasI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SbfI SexAI SfiI
SgfI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
XcmI XhoI XmnI ZraI