(Circular) (Six-base) MAPSORT of: pFN21ASDA0885.seq Check: 8711 from: 1 to: 7123 pFN21ASDA0885 7123 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4933 278 Size: 53 83 186 936 3397 2468 Fragments arranged by size: 3397 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4466 1937 Size: 2529 4594 Acc65I G'GTAC_C Cuts at: 2067 4444 2067 Size: 2377 4746 AclI AA'CG_TT Cuts at: 1563 2469 5250 5623 1563 Size: 906 2781 373 3063 Fragments arranged by size: 3063 2781 906 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2067 2331 2472 3133 3620 5186 6570 6838 Size: 435 264 141 661 487 1566 1384 268 Cuts at: 6838 1632 Size: 1917 Fragments arranged by size: 1917 1566 1384 661 487 435 268 264 141 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6926 AflIII A'CryG_T Cuts at: 1203 6022 6999 1203 Size: 4819 977 1327 Fragments arranged by size: 4819 1327 977 AgeI A'CCGG_T Cuts at: 5954 5954 Size: 7123 AhdI GACnn_n'nnGTC Cuts at: 5852 5852 Size: 7123 AleI CACnn'nnGTG Cuts at: 1166 2661 3150 1166 Size: 1495 489 5139 Fragments arranged by size: 5139 1495 489 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2567 1184 Size: 1383 5740 AlwNI CAG_nnn'CTG Cuts at: 4218 4866 6438 4218 Size: 648 1572 4903 Fragments arranged by size: 4903 1572 648 ApaI G_GGCC'C Cuts at: 4247 4247 Size: 7123 ApaLI G'TGCA_C Cuts at: 5180 6336 5180 Size: 1156 5967 ApoI r'AATT_y Cuts at: 1493 2231 2666 2711 3156 3491 4432 4658 4810 Size: 738 435 45 445 335 941 226 152 Cuts at: 4810 6919 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 941 738 445 435 335 226 152 45 AseI AT'TA_AT Cuts at: 160 1032 5677 160 Size: 872 4645 1606 Fragments arranged by size: 4645 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 AvaI C'yCGr_G Cuts at: 1870 1943 2380 3063 4448 1870 Size: 73 437 683 1385 4545 Fragments arranged by size: 4545 1385 683 437 73 BamHI G'GATC_C Cuts at: 4453 4820 4453 Size: 367 6756 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2067 4444 5899 618 Size: 325 217 627 280 2377 1455 1842 Fragments arranged by size: 2377 1842 1455 627 325 280 217 BanII G_rGCy'C Cuts at: 1391 4247 4442 1391 Size: 2856 195 4072 Fragments arranged by size: 4072 2856 195 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6152 BbvCI CC'TCA_GC Cuts at: 3384 3384 Size: 7123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5314 5348 1903 Size: 34 3377 34 3678 Fragments arranged by size: 3678 3377 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5015 6225 5015 Size: 1210 5913 BclI T'GATC_A Cuts at: 1548 1548 Size: 7123 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5734 136 Size: 107 122 71 631 605 4062 1525 Fragments arranged by size: 4062 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7118 7118 Size: 7123 BlpI GC'TnA_GC Cuts at: 4540 4540 Size: 7123 Bme1580I G_kGCm'C Cuts at: 1790 3272 3314 4247 5184 6340 1790 Size: 1482 42 933 937 1156 2573 Fragments arranged by size: 2573 1482 1156 937 933 42 BmgBI CAC'GTC Cuts at: 1318 2996 1318 Size: 1678 5445 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2112 2268 2841 Size: 416 53 83 399 24 695 156 573 Cuts at: 2841 3704 4854 5812 442 Size: 863 1150 958 1753 Fragments arranged by size: 1753 1150 958 863 695 573 416 399 156 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7123 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2385 2385 Size: 7123 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2355 2505 5783 1239 Size: 1116 150 3278 2579 Fragments arranged by size: 3278 2579 1116 150 Bpu10I CC'TnA_GC Cuts at: 3384 3384 Size: 7123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2754 5248 6113 6411 2754 Size: 2494 865 298 3466 Fragments arranged by size: 3466 2494 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5786 882 Size: 617 4287 2219 Fragments arranged by size: 4287 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4420 4914 493 Size: 711 3216 494 2702 Fragments arranged by size: 3216 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7117 7117 Size: 7123 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4930 5312 5947 275 Size: 53 83 186 936 3397 382 635 1451 Fragments arranged by size: 3397 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5556 5954 6228 6375 6786 5556 Size: 398 274 147 411 5893 Fragments arranged by size: 5893 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2211 2241 3471 3501 4155 Size: 30 165 30 808 30 1230 30 654 Cuts at: 4155 4185 1178 Size: 30 4116 Fragments arranged by size: 4116 1230 808 654 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3459 3853 3905 4254 4314 1186 Size: 2273 394 52 349 60 3995 Fragments arranged by size: 3995 2273 394 349 60 52 BseYI C'CCAG_C Cuts at: 6326 6822 6326 Size: 496 6627 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3768 4231 4401 1163 Size: 573 2032 463 170 3885 Fragments arranged by size: 3885 2032 573 463 170 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4573 5334 6362 1540 Size: 275 186 2572 761 1028 2301 Fragments arranged by size: 2572 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2542 4324 5184 5269 6340 2542 Size: 1782 860 85 1071 3325 Fragments arranged by size: 3325 1782 1071 860 85 BsmI GAATG_Cn' Cuts at: 4634 4727 4634 Size: 93 7030 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2542 3272 3314 4247 4324 4442 5184 Size: 399 752 730 42 933 77 118 742 Cuts at: 5184 5269 6340 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 933 752 742 730 399 118 85 77 42 BspEI T'CCGG_A Cuts at: 6786 6786 Size: 7123 BspHI T'CATG_A Cuts at: 3634 5012 6742 3634 Size: 1378 1730 4015 Fragments arranged by size: 4015 1730 1378 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2208 3517 4478 844 Size: 1364 1309 961 3489 Fragments arranged by size: 3489 1364 1309 961 BsrBI CCG'CTC Cuts at: 1394 1823 5010 1394 Size: 429 3187 3507 Fragments arranged by size: 3507 3187 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2268 2867 5618 5792 65 Size: 1183 1020 599 2751 174 1396 Fragments arranged by size: 2751 1396 1183 1020 599 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5767 5954 1700 Size: 110 3957 187 2869 Fragments arranged by size: 3957 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7123 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7123 BssSI C'ACGA_G Cuts at: 5183 6195 5183 Size: 1012 6111 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7123 BstBI TT'CG_AA Cuts at: 2007 5927 2007 Size: 3920 3203 BstEII G'GTnAC_C Cuts at: 1184 2944 2960 1184 Size: 1760 16 5347 Fragments arranged by size: 5347 1760 16 BstXI CCAn_nnnn'nTGG Cuts at: 2054 3451 2054 Size: 1397 5726 BstYI r'GATC_y Cuts at: 1972 3458 4392 4453 4820 5205 5222 6674 6760 Size: 1486 934 61 367 385 17 1452 86 Cuts at: 6760 6772 7118 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1486 1452 934 385 367 346 86 61 17 12 Bsu36I CC'TnA_GG Cuts at: 3836 3836 Size: 7123 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6571 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2715 487 Size: 683 56 1489 4895 Fragments arranged by size: 4895 1489 683 56 BtsI GCAGTG_nn' Cuts at: 2090 4639 5433 5453 2090 Size: 2549 794 20 3760 Fragments arranged by size: 3760 2549 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7123 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7088 DraI TTT'AAA Cuts at: 4427 4427 Size: 7123 DraIII CAC_nnn'GTG Cuts at: 2096 3775 2096 Size: 1679 5444 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3552 6130 809 Size: 507 2236 2578 1802 Fragments arranged by size: 2578 2236 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3939 4570 5459 Size: 54 1425 50 275 18 2109 631 889 Cuts at: 5459 7088 8 Size: 1629 43 Fragments arranged by size: 2109 1629 1425 889 631 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4570 1537 Size: 275 2758 4090 Fragments arranged by size: 4090 2758 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5053 1353 Size: 3700 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5710 6084 6230 387 Size: 1064 138 4121 374 146 1280 Fragments arranged by size: 4121 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2067 2331 2355 2472 2505 3133 3620 Size: 393 435 264 24 117 33 628 487 Cuts at: 3620 5186 5783 6570 6838 1239 Size: 1566 597 787 268 1524 Fragments arranged by size: 1566 1524 787 628 597 487 435 393 268 264 117 33 24 EcoNI CCTnn'n_nnAGG Cuts at: 3474 3474 Size: 7123 EcoO109I rG'GnC_Cy Cuts at: 4243 4244 4243 Size: 1 7122 EcoRI G'AATT_C Cuts at: 2711 4432 4810 2711 Size: 1721 378 5024 Fragments arranged by size: 5024 1721 378 EcoRV GAT'ATC Cuts at: 2794 2794 Size: 7123 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2183 2695 3595 3664 4132 2183 Size: 512 900 69 468 5174 Fragments arranged by size: 5174 900 512 468 69 FspI TGC'GCA Cuts at: 4043 5629 4043 Size: 1586 5537 HaeII r_GCGC'y Cuts at: 1678 4218 6270 1678 Size: 2540 2052 2531 Fragments arranged by size: 2540 2531 2052 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2017 2049 2351 2383 2921 Size: 32 135 32 445 32 302 32 538 Cuts at: 2921 2953 3206 3238 3832 3864 4177 4209 5764 Size: 32 253 32 594 32 313 32 1555 Cuts at: 5764 5796 5838 5870 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1555 594 538 445 313 302 253 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2124 2890 4467 4713 669 Size: 988 281 186 766 1577 246 3079 Fragments arranged by size: 3079 1577 988 766 281 246 186 HindIII A'AGCT_T Cuts at: 748 748 Size: 7123 HpaI GTT'AAC Cuts at: 2124 4713 2124 Size: 2589 4534 KpnI G_GTAC'C Cuts at: 2071 4448 2071 Size: 2377 4746 MfeI C'AATT_G Cuts at: 3986 4722 3986 Size: 736 6387 MluI A'CGCG_T Cuts at: 6999 6999 Size: 7123 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2417 3875 6237 6421 1305 Size: 1112 1458 2362 184 2007 Fragments arranged by size: 2362 2007 1458 1112 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3941 10 Size: 54 1425 343 2109 3192 Fragments arranged by size: 3192 2109 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2052 2526 2661 3150 3660 5081 5440 Size: 648 886 474 135 489 510 1421 359 Cuts at: 5440 5599 518 Size: 159 2042 Fragments arranged by size: 2042 1421 886 648 510 489 474 359 159 135 MspA1I CmG'CkG Cuts at: 1606 2375 2476 2577 4539 5216 6364 6609 1606 Size: 769 101 101 1962 677 1148 245 2120 Fragments arranged by size: 2120 1962 1148 769 677 245 101 101 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6571 NdeI CA'TA_TG Cuts at: 387 387 Size: 7123 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7123 NotI GC'GGCC_GC Cuts at: 4570 4570 Size: 7123 NspI r_CATG'y Cuts at: 1132 1229 4481 6026 1132 Size: 97 3252 1545 2229 Fragments arranged by size: 3252 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7123 PciI A'CATG_T Cuts at: 6022 6022 Size: 7123 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7123 PmeI GTTT'AAAC Cuts at: 4427 4427 Size: 7123 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5192 5224 6705 6737 4160 Size: 32 1000 32 1481 32 4546 Fragments arranged by size: 4546 1481 1000 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7123 PsiI TTA'TAA Cuts at: 4693 4693 Size: 7123 PspOMI G'GGCC_C Cuts at: 4243 4243 Size: 7123 PstI C_TGCA'G Cuts at: 830 3963 4475 830 Size: 3133 512 3478 Fragments arranged by size: 3478 3133 512 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7123 PvuII CAG'CTG Cuts at: 2375 2476 2375 Size: 101 7022 RsrII CG'GwC_CG Cuts at: 2248 2248 Size: 7123 SalI G'TCGA_C Cuts at: 1936 4465 1936 Size: 2529 4594 SbfI CC_TGCA'GG Cuts at: 4475 4475 Size: 7123 ScaI AGT'ACT Cuts at: 5371 7083 5371 Size: 1712 5411 SfcI C'TryA_G Cuts at: 826 1045 2659 3959 4154 4471 5606 6287 6478 Size: 219 1614 1300 195 317 1135 681 191 Cuts at: 6478 826 Size: 1471 Fragments arranged by size: 1614 1471 1300 1135 681 317 219 195 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7123 SmaI CCC'GGG Cuts at: 1872 2382 4450 1872 Size: 510 2068 4545 Fragments arranged by size: 4545 2068 510 SmlI C'TyrA_G Cuts at: 820 1017 1943 2769 5227 6128 6390 820 Size: 197 926 826 2458 901 262 1553 Fragments arranged by size: 2458 1553 926 901 826 262 197 SnaBI TAC'GTA Cuts at: 493 4420 4914 493 Size: 3927 494 2702 Fragments arranged by size: 3927 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7123 SphI G_CATG'C Cuts at: 1132 4481 1132 Size: 3349 3774 SspI AAT'ATT Cuts at: 5 52 2980 5047 5 Size: 47 2928 2067 2081 Fragments arranged by size: 2928 2081 2067 47 StuI AGG'CCT Cuts at: 1517 3517 1517 Size: 2000 5123 StyI C'CwwG_G Cuts at: 513 1065 2940 3264 3444 3868 4248 4349 4562 Size: 552 1875 324 180 424 380 101 213 Cuts at: 4562 513 Size: 3074 Fragments arranged by size: 3074 1875 552 424 380 324 213 180 101 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2265 3006 3062 5150 5167 5320 5505 Size: 1255 685 741 56 2088 17 153 185 Cuts at: 5505 5950 325 Size: 445 1498 Fragments arranged by size: 2088 1498 1255 741 685 445 185 153 56 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5369 7081 96 Size: 274 80 33 51 1443 3392 1712 138 Fragments arranged by size: 3392 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2319 2351 4155 4187 7083 7115 2319 Size: 32 1804 32 2896 32 2327 Fragments arranged by size: 2896 2327 1804 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3704 1316 Size: 2388 4735 XbaI T'CTAG_A Cuts at: 4459 4459 Size: 7123 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3577 3577 Size: 7123 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7123 XmaI C'CCGG_G Cuts at: 1870 2380 4448 1870 Size: 510 2068 4545 Fragments arranged by size: 4545 2068 510 XmnI GAAnn'nnTTC Cuts at: 5252 5252 Size: 7123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4931 276 Size: 53 83 186 936 3397 2468 Fragments arranged by size: 3397 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI