Restriction map for
pFN21ASDA0883
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AfaI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AviII AvrII BamHI BbeI
BbrPI BbvI BcgI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsiEI BsiHKAI
BsiWI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MslI MunI NarI NciI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsaI RsrII
SacI SacII SalI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TseI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 847 CGCG CG^CG
AhdI 670 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 948 GGATC GGATC(4/5)
AspEI 670 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 871 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 351 CTCGGG C^[CT]CG[GA]G
BalI 583 TGGCCA TGG^CCA
BanI 926 GGTGCC G^G[CT][GA]CC
BbsI 765 GAAGAC GAAGAC(2/6)
BbvCI 460 CCTCAGC CCTCAGC(-5/-2)
BciVI 74 GGATAC GTATCC(6/5)
BglI 626 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 765 GAAGAC GAAGAC(2/6)
BsmBI 1085 GAGACG CGTCTC(1/5)
BsmI 180 GAATGC GAATGC(1/-1)
BsoBI 351 CTCGGG C^[CT]CG[GA]G
BspHI 1077 TCATGA T^CATGA
BsrBI 833 GAGCGG CCGCTC(-3/-3)
BsrDI 445 GCAATG GCAATG(2/0)
BstAPI 654 GCACATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 847 CGCG CG^CG
DraI 126 TTTAAA TTT^AAA
DraIII 655 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 123 GACTTTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 563 CCACGG C^C[GA][CT]GG
Eam1105I 670 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 3 GGCGCT [GA]GCGC^[CT]
MluNI 583 TGGCCA TGG^CCA
MscI 583 TGGCCA TGG^CCA
MspA1I 1068 CCGCTG C[AC]G^C[GT]G
MvnI 847 CGCG CG^CG
NaeI 929 GCCGGC GCC^GGC
NgoMIV 929 GCCGGC G^CCGGC
NspI 453 ACATGC [GA]CATG^[CT]
PflFI 871 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 429 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 535 GAGTC GAGTC(4/5)
PpuMI 110 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1077 TCATGA T^CATGA
SapI 1039 GAAGAGC GCTCTTC(1/4)
SexAI 872 ACCAGGT A^CC[AT]GGT
SmlI 973 CTTGAG C^T[CT][GA]AG
Tsp45I 57 GTCAC ^GT[GC]AC
Tsp509I 419 AATT ^AATT
Tth111I 871 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 429 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 327 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 167 TCCGGA T^CCGGA
827 TCCGGA
AlwNI 463 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
916 CAGATGCTG
ApaI 633 GGGCCC GGGCC^C
954 GGGCCC
BpmI 389 CTCCAG CTGGAG(16/14)
883 CTGGAG
BsaI 348 GGTCTC GGTCTC(1/5)
792 GGTCTC
BseAI 167 TCCGGA T^CCGGA
827 TCCGGA
BseRI 91 GAGGAG GAGGAG(10/8)
1003 GAGGAG
BsgI 27 GTGCAG GTGCAG(16/14)
661 GTGCAG
BsmFI 438 GGGAC GGGAC(10/14)
517 GTCCC
BspEI 167 TCCGGA T^CCGGA
827 TCCGGA
BspMI 140 GCAGGT ACCTGC(4/8)
888 GCAGGT
BsrFI 78 ACCGGC [GA]^CCGG[CT]
929 GCCGGC
BstF5I 852 CATCC GGATG(2/0)
45 GGATG
Cfr10I 78 ACCGGC [GA]^CCGG[CT]
929 GCCGGC
DpnII 273 GATC ^GATC
949 GATC
EcoNI 369 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
882 CCTGGAGCAGG
FauI 957 CCCGC CCCGC(4/6)
630 GCGGG
Fnu4HI 737 GCCGC GC^[ACGT]GC
1067 GCCGC
FokI 852 CATCC GGATG(9/13)
45 GGATG
ItaI 737 GCCGC GC^[ACGT]GC
1067 GCCGC
MboI 273 GATC ^GATC
949 GATC
MroI 167 TCCGGA T^CCGGA
827 TCCGGA
NdeII 273 GATC ^GATC
949 GATC
Sau3AI 273 GATC ^GATC
949 GATC
SfaNI 918 GATGC GCATC(5/9)
407 GCATC
StuI 704 AGGCCT AGG^CCT
1013 AGGCCT
TaqI 363 TCGA T^CGA
1037 TCGA
TfiI 102 GATTC G^A[AT]TC
1074 GAATC
TthHB8I 363 TCGA T^CGA
1037 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AvaII BanII BfaI
BmyI BsaJI BsaWI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EarI EcoO109I EcoRII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI Ksp632I MaeI MaeIII MboII
MnlI MseI MspI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI Tru9I
TspRI