(Circular) (Six-base) MAPSORT of: pFN21ASDA0883.seq Check: 5419 from: 1 to: 5821 pFN21ASDA0883 5821 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 1937 Size: 1227 4594 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2807 2987 3884 5268 5536 1632 Size: 491 684 180 897 1384 268 1917 Fragments arranged by size: 1917 1384 897 684 491 268 180 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 2698 4550 2698 Size: 1852 3969 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2220 1184 Size: 1036 4785 AlwNI CAG_nnn'CTG Cuts at: 2491 2944 3564 5136 2491 Size: 453 620 1572 3176 Fragments arranged by size: 3176 1572 620 453 ApaI G_GGCC'C Cuts at: 2660 2981 2660 Size: 321 5500 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2374 3146 1870 Size: 73 431 772 4545 Fragments arranged by size: 4545 772 431 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2229 2262 2229 Size: 33 5788 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2949 3142 4597 618 Size: 325 217 627 1162 193 1455 1842 Fragments arranged by size: 1842 1455 1162 627 325 217 193 BanII G_rGCy'C Cuts at: 1391 2392 2660 2981 3140 1391 Size: 1001 268 321 159 4072 Fragments arranged by size: 4072 1001 321 268 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2795 928 Size: 971 896 3954 Fragments arranged by size: 3954 971 896 BbvCI CC'TCA_GC Cuts at: 2484 2484 Size: 5821 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2091 3713 4923 2091 Size: 1622 1210 2989 Fragments arranged by size: 2989 1622 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2655 4432 136 Size: 107 122 71 631 605 983 1777 1525 Fragments arranged by size: 1777 1525 983 631 605 122 107 71 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 2427 2660 2981 3882 5038 1790 Size: 637 233 321 901 1156 2573 Fragments arranged by size: 2573 1156 901 637 321 233 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2253 3552 4510 Size: 416 53 83 399 24 836 1299 958 Cuts at: 4510 442 Size: 1753 Fragments arranged by size: 1753 1299 958 836 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2833 2833 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2397 2927 4481 1239 Size: 1158 530 1554 2579 Fragments arranged by size: 2579 1554 1158 530 Bpu10I CC'TnA_GC Cuts at: 2484 2957 2484 Size: 473 5348 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3017 3946 4811 5109 3017 Size: 929 865 298 3729 Fragments arranged by size: 3729 929 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2377 2821 4484 882 Size: 617 878 444 1663 2219 Fragments arranged by size: 2219 1663 878 617 444 BsaAI yAC'GTr Cuts at: 493 1204 3118 3612 493 Size: 711 1914 494 2702 Fragments arranged by size: 2702 1914 711 494 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3628 4010 4645 275 Size: 53 83 186 936 2095 382 635 1451 Fragments arranged by size: 2095 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2190 2508 2850 4254 4652 4926 5073 5484 2190 Size: 318 342 1404 398 274 147 411 2527 Fragments arranged by size: 2527 1404 411 398 342 318 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2393 2423 2799 2829 1178 Size: 30 165 30 990 30 376 30 4170 Fragments arranged by size: 4170 990 376 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 3041 1186 Size: 943 912 3966 Fragments arranged by size: 3966 943 912 BseYI C'CCAG_C Cuts at: 2542 2921 2987 5024 5520 2542 Size: 379 66 2037 496 2843 Fragments arranged by size: 2843 2037 496 379 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2071 2705 1163 Size: 573 335 634 4279 Fragments arranged by size: 4279 634 573 335 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3882 3967 5038 3882 Size: 85 1071 4665 Fragments arranged by size: 4665 1071 85 BsmI GAATG_Cn' Cuts at: 2209 3332 3425 2209 Size: 1123 93 4605 Fragments arranged by size: 4605 1123 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3102 3102 Size: 5821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2392 2427 2660 2981 3140 3882 3967 Size: 399 602 35 233 321 159 742 85 Cuts at: 3967 5038 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 602 399 321 233 159 85 35 BspEI T'CCGG_A Cuts at: 2190 2850 5484 2190 Size: 660 2634 2527 Fragments arranged by size: 2634 2527 660 BspHI T'CATG_A Cuts at: 3100 3710 5440 3100 Size: 610 1730 3481 Fragments arranged by size: 3481 1730 610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2154 2902 3176 844 Size: 1310 748 274 3489 Fragments arranged by size: 3489 1310 748 274 BsrBI CCG'CTC Cuts at: 1394 1823 2858 3708 1394 Size: 429 1035 850 3507 Fragments arranged by size: 3507 1035 850 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2475 4316 4490 65 Size: 1183 1227 1841 174 1396 Fragments arranged by size: 1841 1396 1227 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2101 2952 4465 4652 1700 Size: 110 291 851 1513 187 2869 Fragments arranged by size: 2869 1513 851 291 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 2683 794 Size: 1889 3932 BstBI TT'CG_AA Cuts at: 2007 4625 2007 Size: 2618 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2029 Size: 5821 BstYI r'GATC_y Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816 Size: 1179 367 385 17 1452 86 12 346 Cuts at: 5816 1972 Size: 1977 Fragments arranged by size: 1977 1452 1179 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2586 513 Size: 552 957 564 3748 Fragments arranged by size: 3748 957 564 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 3337 4131 4151 3337 Size: 794 20 5007 Fragments arranged by size: 5007 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 2151 3125 2151 Size: 974 4847 DraIII CAC_nnn'GTG Cuts at: 2683 2683 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2152 4828 809 Size: 507 836 2676 1802 Fragments arranged by size: 2676 1802 836 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2606 3088 Size: 54 1425 50 275 18 420 356 482 Cuts at: 3088 3268 4157 5786 8 Size: 180 889 1629 43 Fragments arranged by size: 1629 1425 889 482 420 356 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2550 2709 3018 3027 3057 3751 1353 Size: 1197 159 309 9 30 694 3423 Fragments arranged by size: 3423 1197 694 309 159 30 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2120 2657 2891 4408 4782 4928 Size: 1064 138 531 537 234 1517 374 146 Cuts at: 4928 387 Size: 1280 Fragments arranged by size: 1517 1280 1064 537 531 374 234 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2397 2807 2927 2987 3884 4481 Size: 393 491 274 410 120 60 897 597 Cuts at: 4481 5268 5536 1239 Size: 787 268 1524 Fragments arranged by size: 1524 897 787 597 491 410 393 274 268 120 60 EcoNI CCTnn'n_nnAGG Cuts at: 2396 2909 2396 Size: 513 5308 EcoO109I rG'GnC_Cy Cuts at: 2134 2656 2657 2977 2134 Size: 522 1 320 4978 Fragments arranged by size: 4978 522 320 1 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2314 2314 Size: 5821 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 2030 4968 1678 Size: 352 2938 2531 Fragments arranged by size: 2938 2531 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2063 2095 2296 2328 2393 Size: 32 135 32 491 32 201 32 65 Cuts at: 2393 2425 2543 2575 2684 2716 2799 2831 4462 Size: 32 118 32 109 32 83 32 1631 Cuts at: 4462 4494 4536 4568 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1631 491 201 135 118 109 83 65 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3165 3411 669 Size: 988 281 1227 246 3079 Fragments arranged by size: 3079 1227 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2556 4935 5119 1305 Size: 1251 2379 184 2007 Fragments arranged by size: 2379 2007 1251 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2608 10 Size: 54 1425 343 776 3223 Fragments arranged by size: 3223 1425 776 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3779 4138 4297 518 Size: 648 2613 359 159 2042 Fragments arranged by size: 2613 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3093 3237 3914 5062 5307 1606 Size: 1487 144 677 1148 245 2120 Fragments arranged by size: 2120 1487 1148 677 245 144 NaeI GCC'GGC Cuts at: 2954 2954 Size: 5821 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4312 Fragments arranged by size: 4312 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2952 2952 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 2480 3179 4724 1132 Size: 97 1251 699 1545 2229 Fragments arranged by size: 2229 1545 1251 699 97 PasI CC'CwG_GG Cuts at: 1348 2392 1348 Size: 1044 4777 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2458 1108 Size: 1350 4471 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 2134 Size: 5821 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PspOMI G'GGCC_C Cuts at: 2656 2977 2656 Size: 321 5500 PstI C_TGCA'G Cuts at: 830 3173 830 Size: 2343 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SapI GCTCTTCn'nnn_ Cuts at: 3057 3057 Size: 5821 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SexAI A'CCwGG_T Cuts at: 2895 2895 Size: 5821 SfcI C'TryA_G Cuts at: 826 1045 3169 4304 4985 5176 826 Size: 219 2124 1135 681 191 1471 Fragments arranged by size: 2124 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2996 3925 4826 5088 820 Size: 197 926 1053 929 901 262 1553 Fragments arranged by size: 1553 1053 929 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3118 3612 493 Size: 2625 494 2702 Fragments arranged by size: 2702 2625 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 2729 3038 1517 Size: 1212 309 4300 Fragments arranged by size: 4300 1212 309 StyI C'CwwG_G Cuts at: 513 1065 2022 3260 513 Size: 552 957 1238 3074 Fragments arranged by size: 3074 1238 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3848 3865 4018 4203 4648 325 Size: 1255 2268 17 153 185 445 1498 Fragments arranged by size: 2268 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4067 5779 96 Size: 274 80 33 51 1443 2090 1712 138 Fragments arranged by size: 2090 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2047 2079 5781 5813 2047 Size: 32 3702 32 2055 Fragments arranged by size: 3702 2055 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2897 1316 Size: 1581 4240 XbaI T'CTAG_A Cuts at: 2350 3157 2350 Size: 807 5014 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI