(Circular) (Six-base) MAPSORT of: pFN21ASDA0881.seq Check: 7142 from: 1 to: 7876 pFN21ASDA0881 7876 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2563 2563 Size: 7876 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2646 5686 278 Size: 53 83 186 936 1110 3040 2468 Fragments arranged by size: 3040 2468 1110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2148 2796 5219 1937 Size: 211 648 2423 4594 Fragments arranged by size: 4594 2423 648 211 Acc65I G'GTAC_C Cuts at: 5197 5197 Size: 7876 AclI AA'CG_TT Cuts at: 1563 6003 6376 1563 Size: 4440 373 3063 Fragments arranged by size: 4440 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 2375 2414 2792 2870 3225 3824 4818 Size: 440 303 39 378 78 355 599 994 Cuts at: 4818 5939 7323 7591 1632 Size: 1121 1384 268 1917 Fragments arranged by size: 1917 1384 1121 994 599 440 378 355 303 268 78 39 AflII C'TTAA_G Cuts at: 820 1017 4376 820 Size: 197 3359 4320 Fragments arranged by size: 4320 3359 197 AflIII A'CryG_T Cuts at: 1203 4292 6775 7752 1203 Size: 3089 2483 977 1327 Fragments arranged by size: 3089 2483 1327 977 AgeI A'CCGG_T Cuts at: 6707 6707 Size: 7876 AhdI GACnn_n'nnGTC Cuts at: 2647 6605 2647 Size: 3958 3918 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7876 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3495 3989 4233 1184 Size: 2311 494 244 4827 Fragments arranged by size: 4827 2311 494 244 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2240 2272 3695 3727 4481 4513 2240 Size: 32 1423 32 754 32 5603 Fragments arranged by size: 5603 1423 754 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2360 2437 3541 4003 4468 4531 5038 5619 7191 Size: 77 1104 462 465 63 507 581 1572 Cuts at: 7191 2360 Size: 3045 Fragments arranged by size: 3045 1572 1104 581 507 465 462 77 63 ApaI G_GGCC'C Cuts at: 4974 5084 5109 4974 Size: 110 25 7741 Fragments arranged by size: 7741 110 25 ApaLI G'TGCA_C Cuts at: 2375 3585 4053 5933 7089 2375 Size: 1210 468 1880 1156 3162 Fragments arranged by size: 3162 1880 1210 1156 468 ApoI r'AATT_y Cuts at: 1493 2788 5185 5411 5563 7672 1493 Size: 1295 2397 226 152 2109 1697 Fragments arranged by size: 2397 2109 1697 1295 226 152 AseI AT'TA_AT Cuts at: 160 1032 6430 160 Size: 872 5398 1606 Fragments arranged by size: 5398 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7876 AvaI C'yCGr_G Cuts at: 1870 1943 2157 2594 3065 3210 5053 5102 5201 Size: 73 214 437 471 145 1843 49 99 Cuts at: 5201 1870 Size: 4545 Fragments arranged by size: 4545 1843 471 437 214 145 99 73 49 BamHI G'GATC_C Cuts at: 5206 5573 5206 Size: 367 7509 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2551 2573 2590 2980 2996 Size: 325 217 627 764 22 17 390 16 Cuts at: 2996 3657 5098 5197 6652 618 Size: 661 1441 99 1455 1842 Fragments arranged by size: 1842 1455 1441 764 661 627 390 325 217 99 22 17 16 BanII G_rGCy'C Cuts at: 1391 2076 2354 3033 3287 3816 4137 4974 5008 Size: 685 278 679 254 529 321 837 34 Cuts at: 5008 5084 5109 5195 1391 Size: 76 25 86 4072 Fragments arranged by size: 4072 837 685 679 529 321 278 254 86 76 34 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2798 928 Size: 971 899 6006 Fragments arranged by size: 6006 971 899 BbvCI CC'TCA_GC Cuts at: 3560 3560 Size: 7876 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5110 5144 6067 6101 1903 Size: 34 3173 34 923 34 3678 Fragments arranged by size: 3678 3173 923 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5768 6978 5768 Size: 1210 6666 BclI T'GATC_A Cuts at: 1548 1548 Size: 7876 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5041 6487 136 Size: 107 122 71 631 605 3369 1446 1525 Fragments arranged by size: 3369 1525 1446 631 605 122 107 71 BglII A'GATC_T Cuts at: 7871 7871 Size: 7876 BlpI GC'TnA_GC Cuts at: 2070 2353 3497 4131 4359 4454 5293 2070 Size: 283 1144 634 228 95 839 4653 Fragments arranged by size: 4653 1144 839 634 283 228 95 Bme1580I G_kGCm'C Cuts at: 1790 2379 2576 3087 3589 4057 4636 4876 4974 Size: 589 197 511 502 468 579 240 98 Cuts at: 4974 5084 5103 5109 5937 7093 1790 Size: 110 19 6 828 1156 2573 Fragments arranged by size: 2573 1156 828 589 579 511 502 468 240 197 110 98 19 6 BmgBI CAC'GTC Cuts at: 1318 4293 1318 Size: 2975 4901 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5607 6565 442 Size: 416 53 83 399 24 4190 958 1753 Fragments arranged by size: 4190 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7876 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3493 3493 Size: 7876 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2741 3091 3323 3569 3782 3800 3866 4091 Size: 1502 350 232 246 213 18 66 225 Cuts at: 4091 4112 4196 4361 4553 4628 4661 4753 4899 Size: 21 84 165 192 75 33 92 146 Cuts at: 4899 4900 6536 1239 Size: 1 1636 2579 Fragments arranged by size: 2579 1636 1502 350 246 232 225 213 192 165 146 92 84 75 66 33 21 18 1 Bpu10I CC'TnA_GC Cuts at: 3560 5111 3560 Size: 1551 6325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2805 3626 4370 4730 6001 6866 7164 2805 Size: 821 744 360 1271 865 298 3517 Fragments arranged by size: 3517 1271 865 821 744 360 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3405 4337 4475 6539 882 Size: 617 1906 932 138 2064 2219 Fragments arranged by size: 2219 2064 1906 932 617 138 BsaAI yAC'GTr Cuts at: 493 1204 5173 5667 493 Size: 711 3969 494 2702 Fragments arranged by size: 3969 2702 711 494 BsaBI GATnn'nnATC Cuts at: 4565 7870 4565 Size: 3305 4571 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2643 4924 5683 6065 Size: 53 83 186 936 1110 2281 759 382 Cuts at: 6065 6700 275 Size: 635 1451 Fragments arranged by size: 2281 1451 1110 936 759 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2166 2784 6309 6707 6981 7128 7539 2166 Size: 618 3525 398 274 147 411 2503 Fragments arranged by size: 3525 2503 618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2240 2253 2270 2283 3695 Size: 30 165 30 837 13 17 13 1412 Cuts at: 3695 3725 4481 4511 1178 Size: 30 756 30 4543 Fragments arranged by size: 4543 1412 837 756 165 30 30 30 30 17 13 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3026 3035 3176 3179 3182 3185 3188 3197 Size: 1840 9 141 3 3 3 3 9 Cuts at: 3197 3200 3203 3590 3716 3824 3914 4673 4884 Size: 3 3 387 126 108 90 759 211 Cuts at: 4884 1186 Size: 4178 Fragments arranged by size: 4178 1840 759 387 211 141 126 108 90 9 9 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2030 3086 3329 3355 3827 4162 4759 4837 4966 Size: 1056 243 26 472 335 597 78 129 Cuts at: 4966 5031 7079 7575 2030 Size: 65 2048 496 2331 Fragments arranged by size: 2331 2048 1056 597 496 472 335 243 129 78 65 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2432 4136 1163 Size: 573 696 1704 4903 Fragments arranged by size: 4903 1704 696 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5326 6087 7115 1540 Size: 275 186 3325 761 1028 2301 Fragments arranged by size: 3325 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 2319 2379 2757 2868 3243 3582 3589 3816 Size: 243 60 378 111 375 339 7 227 Cuts at: 3816 4057 4137 4333 5937 6022 7093 2076 Size: 241 80 196 1604 85 1071 2859 Fragments arranged by size: 2859 1604 1071 378 375 339 243 241 227 196 111 85 80 60 7 BsmI GAATG_Cn' Cuts at: 2713 5387 5480 2713 Size: 2674 93 5109 Fragments arranged by size: 5109 2674 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4283 4938 4283 Size: 655 7221 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2319 2354 2379 2576 2757 2868 Size: 399 286 243 35 25 197 181 111 Cuts at: 2868 3033 3087 3243 3287 3582 3589 3816 4057 Size: 165 54 156 44 295 7 227 241 Cuts at: 4057 4137 4333 4636 4876 4974 5008 5084 5103 Size: 80 196 303 240 98 34 76 19 Cuts at: 5103 5109 5195 5937 6022 7093 1391 Size: 6 86 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 295 286 243 241 240 227 197 196 181 165 156 111 98 86 85 80 76 54 44 35 34 25 19 7 6 BspEI T'CCGG_A Cuts at: 2166 2784 7539 2166 Size: 618 4755 2503 Fragments arranged by size: 4755 2503 618 BspHI T'CATG_A Cuts at: 5765 7495 5765 Size: 1730 6146 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2563 2672 2949 4011 5231 844 Size: 1719 109 277 1062 1220 3489 Fragments arranged by size: 3489 1719 1220 1062 277 109 BsrBI CCG'CTC Cuts at: 1394 1823 2695 2875 3501 3706 4714 5763 1394 Size: 429 872 180 626 205 1008 1049 3507 Fragments arranged by size: 3507 1049 1008 872 626 429 205 180 BsrDI GCAATG_nn' Cuts at: 65 1248 2730 6371 6545 65 Size: 1183 1482 3641 174 1396 Fragments arranged by size: 3641 1482 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3273 6520 6707 1700 Size: 110 1463 3247 187 2869 Fragments arranged by size: 3247 2869 1463 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7876 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7876 BssSI C'ACGA_G Cuts at: 3684 4073 4586 5936 6948 3684 Size: 389 513 1350 1012 4612 Fragments arranged by size: 4612 1350 1012 513 389 BstAPI GCAn_nnn'nTGC Cuts at: 794 3689 4204 794 Size: 2895 515 4466 Fragments arranged by size: 4466 2895 515 BstBI TT'CG_AA Cuts at: 2007 6680 2007 Size: 4673 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7876 BstYI r'GATC_y Cuts at: 1972 2964 3456 4299 4566 5206 5573 5958 5975 Size: 992 492 843 267 640 367 385 17 Cuts at: 5975 7427 7513 7525 7871 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 992 843 640 492 385 367 346 267 86 17 12 BstZ17I GTA'TAC Cuts at: 2149 2149 Size: 7876 Bsu36I CC'TnA_GG Cuts at: 2309 2309 Size: 7876 BtgI C'CryG_G Cuts at: 513 1065 2128 2419 2610 4885 4996 5094 513 Size: 552 1063 291 191 2275 111 98 3295 Fragments arranged by size: 3295 2275 1063 552 291 191 111 98 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2726 487 Size: 683 56 1500 5637 Fragments arranged by size: 5637 1500 683 56 BtsI GCAGTG_nn' Cuts at: 3893 3953 4055 4634 5068 5074 5392 6186 6206 Size: 60 102 579 434 6 318 794 20 Cuts at: 6206 3893 Size: 5563 Fragments arranged by size: 5563 794 579 434 318 102 60 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7876 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7841 DraI TTT'AAA Cuts at: 5180 5180 Size: 7876 DraIII CAC_nnn'GTG Cuts at: 3082 4985 4997 3082 Size: 1903 12 5961 Fragments arranged by size: 5961 1903 12 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6883 809 Size: 507 5567 1802 Fragments arranged by size: 5567 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2125 2184 2607 Size: 54 1425 50 275 18 295 59 423 Cuts at: 2607 2876 3219 5323 6212 7841 8 Size: 269 343 2104 889 1629 43 Fragments arranged by size: 2104 1629 1425 889 423 343 295 275 269 59 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5323 1537 Size: 275 3511 4090 Fragments arranged by size: 4090 3511 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2108 3012 3153 3174 3382 4386 4650 Size: 728 27 904 141 21 208 1004 264 Cuts at: 4650 5806 1353 Size: 1156 3423 Fragments arranged by size: 3423 1156 1004 904 728 264 208 141 27 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3911 4436 5016 6463 6837 6983 Size: 1064 138 2322 525 580 1447 374 146 Cuts at: 6983 387 Size: 1280 Fragments arranged by size: 2322 1447 1280 1064 580 525 374 146 138 EcoICRI GAG'CTC Cuts at: 2074 3814 4135 2074 Size: 1740 321 5815 Fragments arranged by size: 5815 1740 321 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2375 2414 2741 2792 2870 3091 Size: 393 440 303 39 327 51 78 221 Cuts at: 3091 3225 3323 3569 3782 3800 3824 3866 4091 Size: 134 98 246 213 18 24 42 225 Cuts at: 4091 4112 4196 4361 4553 4628 4661 4753 4818 Size: 21 84 165 192 75 33 92 65 Cuts at: 4818 4899 4900 5939 6536 7323 7591 1239 Size: 81 1 1039 597 787 268 1524 Fragments arranged by size: 1524 1039 787 597 440 393 327 303 268 246 225 221 213 192 165 134 98 92 84 81 78 75 65 51 42 39 33 24 21 18 1 EcoNI CCTnn'n_nnAGG Cuts at: 2393 3761 3995 4899 2393 Size: 1368 234 904 5370 Fragments arranged by size: 5370 1368 904 234 EcoO109I rG'GnC_Cy Cuts at: 2056 2434 3004 3200 4000 4015 4504 4952 4970 Size: 378 570 196 800 15 489 448 18 Cuts at: 4970 4971 5081 5106 2056 Size: 1 110 25 4826 Fragments arranged by size: 4826 800 570 489 448 378 196 110 25 18 15 1 EcoRI G'AATT_C Cuts at: 5185 5563 5185 Size: 378 7498 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2875 2875 Size: 7876 FspI TGC'GCA Cuts at: 6382 6382 Size: 7876 HaeII r_GCGC'y Cuts at: 1678 2429 3105 3931 4469 7023 1678 Size: 751 676 826 538 2554 2531 Fragments arranged by size: 2554 2531 826 751 676 538 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2633 2665 4291 4323 6517 Size: 32 135 32 1061 32 1626 32 2194 Cuts at: 6517 6549 6591 6623 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2194 1626 1061 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2797 3127 5220 5466 669 Size: 988 281 859 330 2093 246 3079 Fragments arranged by size: 3079 2093 988 859 330 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7876 HpaI GTT'AAC Cuts at: 5466 5466 Size: 7876 KpnI G_GTAC'C Cuts at: 5201 5201 Size: 7876 MfeI C'AATT_G Cuts at: 5475 5475 Size: 7876 MluI A'CGCG_T Cuts at: 7752 7752 Size: 7876 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6990 7174 1305 Size: 5685 184 2007 Fragments arranged by size: 5685 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2127 2186 2609 3221 10 Size: 54 1425 343 295 59 423 612 4665 Fragments arranged by size: 4665 1425 612 423 343 295 59 54 MslI CAynn'nnrTG Cuts at: 518 1166 2472 5834 6193 6352 518 Size: 648 1306 3362 359 159 2042 Fragments arranged by size: 3362 2042 1306 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2411 3496 3538 3598 3616 3802 4174 Size: 424 381 1085 42 60 18 186 372 Cuts at: 4174 4648 5096 5292 5969 7117 7362 1606 Size: 474 448 196 677 1148 245 2120 Fragments arranged by size: 2120 1148 1085 677 474 448 424 381 372 245 196 186 60 42 18 NcoI C'CATG_G Cuts at: 513 1065 2128 2610 4885 513 Size: 552 1063 482 2275 3504 Fragments arranged by size: 3504 2275 1063 552 482 NdeI CA'TA_TG Cuts at: 387 387 Size: 7876 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7876 NotI GC'GGCC_GC Cuts at: 5323 5323 Size: 7876 NspI r_CATG'y Cuts at: 1132 1229 3040 3094 4220 4909 5234 6779 1132 Size: 97 1811 54 1126 689 325 1545 2229 Fragments arranged by size: 2229 1811 1545 1126 689 325 97 54 PasI CC'CwG_GG Cuts at: 1348 4810 1348 Size: 3462 4414 PciI A'CATG_T Cuts at: 6775 6775 Size: 7876 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7876 PfoI T'CCnGG_A Cuts at: 2115 2868 3459 3816 4284 2115 Size: 753 591 357 468 5707 Fragments arranged by size: 5707 753 591 468 357 PmeI GTTT'AAAC Cuts at: 5180 5180 Size: 7876 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2240 2272 3695 3727 4481 4513 5945 5977 7458 Size: 32 1423 32 754 32 1432 32 1481 Cuts at: 7458 7490 2240 Size: 32 2626 Fragments arranged by size: 2626 1481 1432 1423 754 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 4000 4015 2056 Size: 1944 15 5917 Fragments arranged by size: 5917 1944 15 PshAI GACnn'nnGTC Cuts at: 1613 2770 2802 1613 Size: 1157 32 6687 Fragments arranged by size: 6687 1157 32 PsiI TTA'TAA Cuts at: 5446 5446 Size: 7876 PspOMI G'GGCC_C Cuts at: 4970 5080 5105 4970 Size: 110 25 7741 Fragments arranged by size: 7741 110 25 PstI C_TGCA'G Cuts at: 830 2946 3352 3597 3948 4149 4269 4677 5228 Size: 2116 406 245 351 201 120 408 551 Cuts at: 5228 830 Size: 3478 Fragments arranged by size: 3478 2116 551 408 406 351 245 201 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7876 PvuII CAG'CTG Cuts at: 2030 2411 3496 3538 3802 4174 2030 Size: 381 1085 42 264 372 5732 Fragments arranged by size: 5732 1085 381 372 264 42 SacI G_AGCT'C Cuts at: 2076 3816 4137 2076 Size: 1740 321 5815 Fragments arranged by size: 5815 1740 321 SacII CC_GC'GG Cuts at: 5097 5097 Size: 7876 SalI G'TCGA_C Cuts at: 1936 2795 5218 1936 Size: 859 2423 4594 Fragments arranged by size: 4594 2423 859 SapI GCTCTTCn'nnn_ Cuts at: 2081 2108 4386 2081 Size: 27 2278 5571 Fragments arranged by size: 5571 2278 27 SbfI CC_TGCA'GG Cuts at: 5228 5228 Size: 7876 ScaI AGT'ACT Cuts at: 2780 3935 6124 7836 2780 Size: 1155 2189 1712 2820 Fragments arranged by size: 2820 2189 1712 1155 SfcI C'TryA_G Cuts at: 826 1045 2203 2942 3348 3593 3944 4145 4265 Size: 219 1158 739 406 245 351 201 120 Cuts at: 4265 4673 5224 6359 7040 7231 826 Size: 408 551 1135 681 191 1471 Fragments arranged by size: 1471 1158 1135 739 681 551 408 406 351 245 219 201 191 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7876 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7876 SmaI CCC'GGG Cuts at: 1872 2159 3212 5104 5203 1872 Size: 287 1053 1892 99 4545 Fragments arranged by size: 4545 1892 1053 287 99 SmlI C'TyrA_G Cuts at: 820 1017 1943 2820 3065 3605 4376 4385 4709 Size: 197 926 877 245 540 771 9 324 Cuts at: 4709 5980 6881 7143 820 Size: 1271 901 262 1553 Fragments arranged by size: 1553 1271 926 901 877 771 540 324 262 245 197 9 SnaBI TAC'GTA Cuts at: 493 5173 5667 493 Size: 4680 494 2702 Fragments arranged by size: 4680 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7876 SphI G_CATG'C Cuts at: 1132 4220 4909 5234 1132 Size: 3088 689 325 3774 Fragments arranged by size: 3774 3088 689 325 SrfI GCCC'GGGC Cuts at: 5104 5104 Size: 7876 SspI AAT'ATT Cuts at: 5 52 5800 5 Size: 47 5748 2081 Fragments arranged by size: 5748 2081 47 StuI AGG'CCT Cuts at: 1517 4736 1517 Size: 3219 4657 StyI C'CwwG_G Cuts at: 513 1065 2128 2610 2652 2913 3727 4885 5315 Size: 552 1063 482 42 261 814 1158 430 Cuts at: 5315 513 Size: 3074 Fragments arranged by size: 3074 1158 1063 814 552 482 430 261 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2896 2998 5903 5920 6073 6258 6703 Size: 1255 1316 102 2905 17 153 185 445 Cuts at: 6703 325 Size: 1498 Fragments arranged by size: 2905 1498 1316 1255 445 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2778 3933 3957 Size: 274 80 33 51 1443 801 1155 24 Cuts at: 3957 4179 4314 6122 7834 96 Size: 222 135 1808 1712 138 Fragments arranged by size: 1808 1712 1443 1155 801 274 222 138 135 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4034 4066 4981 5013 5150 5182 7836 7868 4034 Size: 32 915 32 137 32 2654 32 4042 Fragments arranged by size: 4042 2654 915 137 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7876 XbaI T'CTAG_A Cuts at: 2368 5212 2368 Size: 2844 5032 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2135 2566 2890 4087 4777 5144 2135 Size: 431 324 1197 690 367 4867 Fragments arranged by size: 4867 1197 690 431 367 324 XhoI C'TCGA_G Cuts at: 1943 3065 1943 Size: 1122 6754 XmaI C'CCGG_G Cuts at: 1870 2157 3210 5102 5201 1870 Size: 287 1053 1892 99 4545 Fragments arranged by size: 4545 1892 1053 287 99 XmnI GAAnn'nnTTC Cuts at: 4280 6005 4280 Size: 1725 6151 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2644 5684 276 Size: 53 83 186 936 1110 3040 2468 Fragments arranged by size: 3040 2468 1110 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SwaI