(Circular) (Six-base) MAPSORT of: pFN21ASDA0876.seq Check: 5716 from: 1 to: 8017 pFN21ASDA0876 8017 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2233 4736 5272 2233 Size: 2503 536 4978 Fragments arranged by size: 4978 2503 536 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5827 278 Size: 53 83 186 936 4291 2468 Fragments arranged by size: 4291 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5360 1937 Size: 3423 4594 Acc65I G'GTAC_C Cuts at: 5338 5338 Size: 8017 AclI AA'CG_TT Cuts at: 1563 6144 6517 1563 Size: 4581 373 3063 Fragments arranged by size: 4581 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2779 2795 3137 6080 7464 7732 1632 Size: 1147 16 342 2943 1384 268 1917 Fragments arranged by size: 2943 1917 1384 1147 342 268 16 AfeI AGC'GCT Cuts at: 3008 3219 3008 Size: 211 7806 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7820 AflIII A'CryG_T Cuts at: 1203 2979 3654 4373 6916 7893 1203 Size: 1776 675 719 2543 977 1327 Fragments arranged by size: 2543 1776 1327 977 719 675 AgeI A'CCGG_T Cuts at: 6848 6848 Size: 8017 AhdI GACnn_n'nnGTC Cuts at: 6746 6746 Size: 8017 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8017 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4341 1184 Size: 3157 4860 AlwNI CAG_nnn'CTG Cuts at: 3304 3503 4052 5760 7332 3304 Size: 199 549 1708 1572 3989 Fragments arranged by size: 3989 1708 1572 549 199 ApaI G_GGCC'C Cuts at: 3497 3497 Size: 8017 ApaLI G'TGCA_C Cuts at: 2941 6074 7230 2941 Size: 3133 1156 3728 Fragments arranged by size: 3728 3133 1156 ApoI r'AATT_y Cuts at: 1493 2093 2822 5326 5552 5704 7813 1493 Size: 600 729 2504 226 152 2109 1697 Fragments arranged by size: 2504 2109 1697 729 600 226 152 AseI AT'TA_AT Cuts at: 160 1032 6571 160 Size: 872 5539 1606 Fragments arranged by size: 5539 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8017 AvaI C'yCGr_G Cuts at: 1870 1943 2708 3002 3080 3105 3275 3789 4349 Size: 73 765 294 78 25 170 514 560 Cuts at: 4349 4496 4553 5173 5342 1870 Size: 147 57 620 169 4545 Fragments arranged by size: 4545 765 620 560 514 294 170 169 147 78 73 57 25 AvrII C'CTAG_G Cuts at: 4783 4783 Size: 8017 BamHI G'GATC_C Cuts at: 2787 4467 5347 5714 2787 Size: 1680 880 367 5090 Fragments arranged by size: 5090 1680 880 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2230 2355 2519 3532 Size: 325 217 627 255 188 125 164 1013 Cuts at: 3532 3559 3616 3808 3855 4129 4613 4844 5152 Size: 27 57 192 47 274 484 231 308 Cuts at: 5152 5213 5338 6793 618 Size: 61 125 1455 1842 Fragments arranged by size: 1842 1455 1013 627 484 325 308 274 255 231 217 192 188 164 125 125 61 57 47 27 BanII G_rGCy'C Cuts at: 1391 2143 2717 3092 3483 3497 3524 4068 4977 Size: 752 574 375 391 14 27 544 909 Cuts at: 4977 5166 5305 5336 1391 Size: 189 139 31 4072 Fragments arranged by size: 4072 909 752 574 544 391 375 189 139 31 27 14 BbeI G_GCGC'C Cuts at: 2046 2234 3859 2046 Size: 188 1625 6204 Fragments arranged by size: 6204 1625 188 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2576 3186 4196 928 Size: 971 677 610 1010 4749 Fragments arranged by size: 4749 1010 971 677 610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2509 2543 6208 6242 1903 Size: 34 572 34 3665 34 3678 Fragments arranged by size: 3678 3665 572 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4631 5909 7119 4631 Size: 1278 1210 5529 Fragments arranged by size: 5529 1278 1210 BclI T'GATC_A Cuts at: 1548 2829 1548 Size: 1281 6736 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2147 2262 6628 Size: 107 122 71 631 605 475 115 4366 Cuts at: 6628 136 Size: 1525 Fragments arranged by size: 4366 1525 631 605 475 122 115 107 71 BglII A'GATC_T Cuts at: 2967 8012 2967 Size: 5045 2972 BlpI GC'TnA_GC Cuts at: 3086 5434 3086 Size: 2348 5669 Bme1580I G_kGCm'C Cuts at: 1790 2945 3497 3552 3564 5155 5216 6078 7234 Size: 1155 552 55 12 1591 61 862 1156 Cuts at: 7234 1790 Size: 2573 Fragments arranged by size: 2573 1591 1156 1155 862 552 61 55 12 BmgBI CAC'GTC Cuts at: 1318 2218 2458 2980 4374 4650 1318 Size: 900 240 522 1394 276 4685 Fragments arranged by size: 4685 1394 900 522 276 240 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2458 3558 5748 Size: 416 53 83 399 24 1041 1100 2190 Cuts at: 5748 6706 442 Size: 958 1753 Fragments arranged by size: 2190 1753 1100 1041 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8017 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3106 4660 3106 Size: 1554 6463 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2702 3115 3497 5120 6677 1239 Size: 1463 413 382 1623 1557 2579 Fragments arranged by size: 2579 1623 1557 1463 413 382 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3901 3997 4718 6142 7007 7305 3901 Size: 96 721 1424 865 298 4613 Fragments arranged by size: 4613 1424 865 721 298 96 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3144 3773 4723 6680 882 Size: 617 1645 629 950 1957 2219 Fragments arranged by size: 2219 1957 1645 950 629 617 BsaAI yAC'GTr Cuts at: 493 1204 2116 2946 3613 3655 4474 4660 5257 Size: 711 912 830 667 42 819 186 597 Cuts at: 5257 5314 5808 493 Size: 57 494 2702 Fragments arranged by size: 2702 912 830 819 711 667 597 494 186 57 42 BsaBI GATnn'nnATC Cuts at: 3839 8011 3839 Size: 4172 3845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 2231 2730 3856 Size: 53 83 186 936 510 188 499 1126 Cuts at: 3856 3972 5824 6206 6841 275 Size: 116 1852 382 635 1451 Fragments arranged by size: 1852 1451 1126 936 635 510 499 382 188 186 116 83 53 BsaWI w'CCGG_w Cuts at: 2484 3144 4977 6450 6848 7122 7269 7680 2484 Size: 660 1833 1473 398 274 147 411 2821 Fragments arranged by size: 2821 1833 1473 660 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2498 2528 2627 2657 3443 Size: 30 165 30 1095 30 99 30 786 Cuts at: 3443 3473 3641 3671 4871 4901 4964 4994 1178 Size: 30 168 30 1200 30 63 30 4201 Fragments arranged by size: 4201 1200 1095 786 168 165 99 63 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3119 3242 3266 3293 3296 3299 3302 3305 Size: 1933 123 24 27 3 3 3 3 Cuts at: 3305 3464 3530 3584 3941 4446 5008 5117 5120 Size: 159 66 54 357 505 562 109 3 Cuts at: 5120 5195 1186 Size: 75 4008 Fragments arranged by size: 4008 1933 562 505 357 159 123 109 75 66 54 27 24 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2054 3209 3233 3257 3490 4942 5064 7220 7716 Size: 1155 24 24 233 1452 122 2156 496 Cuts at: 7716 2054 Size: 2355 Fragments arranged by size: 2355 2156 1452 1155 496 233 122 24 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2609 3771 4066 5306 1163 Size: 573 873 1162 295 1240 3874 Fragments arranged by size: 3874 1240 1162 873 573 295 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 3626 3875 4199 5467 6228 Size: 275 186 1315 310 249 324 1268 761 Cuts at: 6228 7256 1540 Size: 1028 2301 Fragments arranged by size: 2301 1315 1268 1028 761 324 310 275 249 186 BsiHKAI G_wGCw'C Cuts at: 2670 2717 2945 3092 3654 3681 4631 4641 4689 Size: 47 228 147 562 27 950 10 48 Cuts at: 4689 4977 5147 6078 6163 7234 2670 Size: 288 170 931 85 1071 3453 Fragments arranged by size: 3453 1071 950 931 562 288 228 170 147 85 48 47 27 10 BsmI GAATG_Cn' Cuts at: 5528 5621 5528 Size: 93 7924 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2670 2717 2945 3092 3483 3497 Size: 399 353 527 47 228 147 391 14 Cuts at: 3497 3524 3552 3564 3654 3681 4068 4631 4641 Size: 27 28 12 90 27 387 563 10 Cuts at: 4641 4689 4977 5147 5155 5166 5216 5305 5336 Size: 48 288 170 8 11 50 89 31 Cuts at: 5336 6078 6163 7234 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 563 527 399 391 387 353 288 228 170 147 90 89 85 50 48 47 31 28 27 27 14 12 11 10 8 BspEI T'CCGG_A Cuts at: 2484 7680 2484 Size: 5196 2821 BspHI T'CATG_A Cuts at: 2067 2826 5906 7636 2067 Size: 759 3080 1730 2448 Fragments arranged by size: 3080 2448 1730 759 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2233 2631 3126 3758 4309 4434 4673 4736 Size: 1389 398 495 632 551 125 239 63 Cuts at: 4736 4868 5272 5372 844 Size: 132 404 100 3489 Fragments arranged by size: 3489 1389 632 551 495 404 398 239 132 125 100 63 BsrBI CCG'CTC Cuts at: 1394 1823 2686 3382 4201 5127 5904 1394 Size: 429 863 696 819 926 777 3507 Fragments arranged by size: 3507 926 863 819 777 696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6512 6686 65 Size: 1183 5264 174 1396 Fragments arranged by size: 5264 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2855 3529 3852 3870 5292 6661 6848 Size: 110 1045 674 323 18 1422 1369 187 Cuts at: 6848 1700 Size: 2869 Fragments arranged by size: 2869 1422 1369 1045 674 323 187 110 18 BsrGI T'GTAC_A Cuts at: 96 2607 96 Size: 2511 5506 BssHII G'CGCG_C Cuts at: 1925 4055 4502 1925 Size: 2130 447 5440 Fragments arranged by size: 5440 2130 447 BssSI C'ACGA_G Cuts at: 4921 6077 7089 4921 Size: 1156 1012 5849 Fragments arranged by size: 5849 1156 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3304 4052 4311 794 Size: 2510 748 259 4500 Fragments arranged by size: 4500 2510 748 259 BstBI TT'CG_AA Cuts at: 2007 6821 2007 Size: 4814 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8017 BstXI CCAn_nnnn'nTGG Cuts at: 2305 2305 Size: 8017 BstYI r'GATC_y Cuts at: 1972 2787 2967 4467 5347 5714 6099 6116 7568 Size: 815 180 1500 880 367 385 17 1452 Cuts at: 7568 7654 7666 8012 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1500 1452 880 815 385 367 346 180 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3555 4769 3555 Size: 1214 6803 BtgI C'CryG_G Cuts at: 513 1065 2022 2038 2085 2189 2351 2973 3063 Size: 552 957 16 47 104 162 622 90 Cuts at: 3063 3510 3620 3723 5226 513 Size: 447 110 103 1503 3304 Fragments arranged by size: 3304 1503 957 622 552 447 162 110 104 103 90 47 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4471 487 Size: 683 56 3245 4033 Fragments arranged by size: 4033 3245 683 56 BtsI GCAGTG_nn' Cuts at: 3302 3766 4004 4639 5533 6327 6347 3302 Size: 464 238 635 894 794 20 4972 Fragments arranged by size: 4972 894 794 635 464 238 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8017 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7982 DraI TTT'AAA Cuts at: 2098 5321 2098 Size: 3223 4794 DraIII CAC_nnn'GTG Cuts at: 2940 4534 2940 Size: 1594 6423 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2257 7024 809 Size: 507 941 4767 1802 Fragments arranged by size: 4767 1802 941 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2325 2691 Size: 54 1425 50 275 18 327 168 366 Cuts at: 2691 2931 3313 3359 3623 3872 4014 4161 5464 Size: 240 382 46 264 249 142 147 1303 Cuts at: 5464 6353 7982 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1303 889 382 366 327 275 264 249 240 168 147 142 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 3623 3872 5464 1537 Size: 275 1501 310 249 1592 4090 Fragments arranged by size: 4090 1592 1501 310 275 249 EarI CTCTTCn'nnn_ Cuts at: 1353 2273 3330 3386 3842 4094 5947 1353 Size: 920 1057 56 456 252 1853 3423 Fragments arranged by size: 3423 1853 1057 920 456 252 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5257 6604 6978 7124 387 Size: 1064 138 3668 1347 374 146 1280 Fragments arranged by size: 3668 1347 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2715 3090 4975 2715 Size: 375 1885 5757 Fragments arranged by size: 5757 1885 375 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2702 2779 2795 3115 3137 3497 5120 Size: 393 1070 77 16 320 22 360 1623 Cuts at: 5120 6080 6677 7464 7732 1239 Size: 960 597 787 268 1524 Fragments arranged by size: 1623 1524 1070 960 787 597 393 360 320 268 77 22 16 EcoNI CCTnn'n_nnAGG Cuts at: 3227 4148 4787 5000 5102 5279 3227 Size: 921 639 213 102 177 5965 Fragments arranged by size: 5965 921 639 213 177 102 EcoO109I rG'GnC_Cy Cuts at: 3034 3500 4108 4946 5061 3034 Size: 466 608 838 115 5990 Fragments arranged by size: 5990 838 608 466 115 EcoRI G'AATT_C Cuts at: 2822 5326 5704 2822 Size: 2504 378 5135 Fragments arranged by size: 5135 2504 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2593 3412 3655 2593 Size: 819 243 6955 Fragments arranged by size: 6955 819 243 FseI GG_CCGG'CC Cuts at: 3874 5296 3874 Size: 1422 6595 FspI TGC'GCA Cuts at: 2881 2990 6523 2881 Size: 109 3533 4375 Fragments arranged by size: 4375 3533 109 FspAI rTGC'GCAy Cuts at: 2990 2990 Size: 8017 HaeII r_GCGC'y Cuts at: 1678 2046 2234 2680 3010 3071 3221 3458 3859 Size: 368 188 446 330 61 150 237 401 Cuts at: 3859 4528 4548 5203 7164 1678 Size: 669 20 655 1961 2531 Fragments arranged by size: 2531 1961 669 655 446 401 368 330 237 188 150 61 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2627 2659 3641 3673 3917 Size: 32 135 32 1055 32 982 32 244 Cuts at: 3917 3949 4188 4220 4871 4903 5045 5077 6658 Size: 32 239 32 651 32 142 32 1581 Cuts at: 6658 6690 6732 6764 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1581 1055 982 651 244 239 142 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3271 5361 5607 669 Size: 988 281 1333 2090 246 3079 Fragments arranged by size: 3079 2090 1333 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8017 HpaI GTT'AAC Cuts at: 5607 5607 Size: 8017 KasI G'GCGC_C Cuts at: 2042 2230 3855 2042 Size: 188 1625 6204 Fragments arranged by size: 6204 1625 188 KpnI G_GTAC'C Cuts at: 5342 5342 Size: 8017 MfeI C'AATT_G Cuts at: 5616 5616 Size: 8017 MluI A'CGCG_T Cuts at: 7893 7893 Size: 8017 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3722 4786 7131 7315 1305 Size: 2417 1064 2345 184 2007 Fragments arranged by size: 2417 2345 2007 1064 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2159 2327 2693 2933 10 Size: 54 1425 343 327 168 366 240 5094 Fragments arranged by size: 5094 1425 366 343 327 240 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 2303 2978 3602 5975 6334 6493 518 Size: 648 1137 675 624 2373 359 159 2042 Fragments arranged by size: 2373 2042 1137 675 648 624 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2191 2353 2436 3418 3622 3985 4049 Size: 452 133 162 83 982 204 363 64 Cuts at: 4049 4249 4561 4830 4942 5068 5433 6110 7258 Size: 200 312 269 112 126 365 677 1148 Cuts at: 7258 7503 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 982 677 452 365 363 312 269 245 204 200 162 133 126 112 83 64 NaeI GCC'GGC Cuts at: 2857 3531 3854 3872 5294 2857 Size: 674 323 18 1422 5580 Fragments arranged by size: 5580 1422 674 323 18 NarI GG'CG_CC Cuts at: 2043 2231 3856 2043 Size: 188 1625 6204 Fragments arranged by size: 6204 1625 188 NcoI C'CATG_G Cuts at: 513 1065 2022 2085 2973 3510 3723 513 Size: 552 957 63 888 537 213 4807 Fragments arranged by size: 4807 957 888 552 537 213 63 NdeI CA'TA_TG Cuts at: 387 387 Size: 8017 NgoMIV G'CCGG_C Cuts at: 2855 3529 3852 3870 5292 2855 Size: 674 323 18 1422 5580 Fragments arranged by size: 5580 1422 674 323 18 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8017 NotI GC'GGCC_GC Cuts at: 5464 5464 Size: 8017 NspI r_CATG'y Cuts at: 1132 1229 2566 5375 6920 1132 Size: 97 1337 2809 1545 2229 Fragments arranged by size: 2809 2229 1545 1337 97 PasI CC'CwG_GG Cuts at: 1348 3194 3865 1348 Size: 1846 671 5500 Fragments arranged by size: 5500 1846 671 PciI A'CATG_T Cuts at: 6916 6916 Size: 8017 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 3865 4534 1108 Size: 1137 1620 669 4591 Fragments arranged by size: 4591 1620 1137 669 PfoI T'CCnGG_A Cuts at: 2493 3092 2493 Size: 599 7418 PmeI GTTT'AAAC Cuts at: 5321 5321 Size: 8017 PmlI CAC'GTG Cuts at: 2946 3655 4474 4660 2946 Size: 709 819 186 6303 Fragments arranged by size: 6303 819 709 186 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4022 4054 6086 6118 7599 7631 4022 Size: 32 2032 32 1481 32 4408 Fragments arranged by size: 4408 2032 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3034 4946 5061 3034 Size: 1912 115 5990 Fragments arranged by size: 5990 1912 115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8017 PsiI TTA'TAA Cuts at: 5587 5587 Size: 8017 PspOMI G'GGCC_C Cuts at: 3493 3493 Size: 8017 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2531 2563 2531 Size: 32 7985 PstI C_TGCA'G Cuts at: 830 3000 3508 3999 4320 4446 5283 5369 830 Size: 2170 508 491 321 126 837 86 3478 Fragments arranged by size: 3478 2170 837 508 491 321 126 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8017 PvuII CAG'CTG Cuts at: 2058 3418 4942 5068 2058 Size: 1360 1524 126 5007 Fragments arranged by size: 5007 1524 1360 126 SacI G_AGCT'C Cuts at: 2717 3092 4977 2717 Size: 375 1885 5757 Fragments arranged by size: 5757 1885 375 SacII CC_GC'GG Cuts at: 2192 2354 3623 2192 Size: 162 1269 6586 Fragments arranged by size: 6586 1269 162 SalI G'TCGA_C Cuts at: 1936 5359 1936 Size: 3423 4594 SanDI GG'GwC_CC Cuts at: 4946 5061 4946 Size: 115 7902 SapI GCTCTTCn'nnn_ Cuts at: 3386 3842 4094 3386 Size: 456 252 7309 Fragments arranged by size: 7309 456 252 SbfI CC_TGCA'GG Cuts at: 4320 5283 5369 4320 Size: 963 86 6968 Fragments arranged by size: 6968 963 86 ScaI AGT'ACT Cuts at: 2342 6265 7977 2342 Size: 3923 1712 2382 Fragments arranged by size: 3923 2382 1712 SexAI A'CCwGG_T Cuts at: 5040 5040 Size: 8017 SfcI C'TryA_G Cuts at: 826 1045 2996 3504 3995 4112 4316 4442 4891 Size: 219 1951 508 491 117 204 126 449 Cuts at: 4891 5279 5365 6500 7181 7372 826 Size: 388 86 1135 681 191 1471 Fragments arranged by size: 1951 1471 1135 681 508 491 449 388 219 204 191 126 117 86 SfiI GGCCn_nnn'nGGCC Cuts at: 2262 2262 Size: 8017 SfoI GGC'GCC Cuts at: 2044 2232 3857 2044 Size: 188 1625 6204 Fragments arranged by size: 6204 1625 188 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8017 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8017 SmaI CCC'GGG Cuts at: 1872 2710 3107 3791 5344 1872 Size: 838 397 684 1553 4545 Fragments arranged by size: 4545 1553 838 684 397 SmlI C'TyrA_G Cuts at: 820 1017 1943 3916 3976 4733 6121 7022 7284 Size: 197 926 1973 60 757 1388 901 262 Cuts at: 7284 820 Size: 1553 Fragments arranged by size: 1973 1553 1388 926 901 757 262 197 60 SnaBI TAC'GTA Cuts at: 493 5314 5808 493 Size: 4821 494 2702 Fragments arranged by size: 4821 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8017 SphI G_CATG'C Cuts at: 1132 5375 1132 Size: 4243 3774 SrfI GCCC'GGGC Cuts at: 3791 3791 Size: 8017 SspI AAT'ATT Cuts at: 5 52 5941 5 Size: 47 5889 2081 Fragments arranged by size: 5889 2081 47 StuI AGG'CCT Cuts at: 1517 3671 3914 1517 Size: 2154 243 5620 Fragments arranged by size: 5620 2154 243 StyI C'CwwG_G Cuts at: 513 1065 2022 2085 2973 3366 3510 3723 4783 Size: 552 957 63 888 393 144 213 1060 Cuts at: 4783 5187 5456 513 Size: 404 269 3074 Fragments arranged by size: 3074 1060 957 888 552 404 393 269 213 144 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4147 6044 6061 6214 6399 6844 325 Size: 1255 2567 1897 17 153 185 445 1498 Fragments arranged by size: 2567 1897 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 2340 2553 Size: 274 80 33 51 1443 300 63 213 Cuts at: 2553 2607 4581 4821 6263 7975 96 Size: 54 1974 240 1442 1712 138 Fragments arranged by size: 1974 1712 1443 1442 300 274 240 213 138 80 63 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2498 2530 3443 3475 4964 4996 7977 8009 2498 Size: 32 913 32 1489 32 2981 32 2506 Fragments arranged by size: 2981 2506 1489 913 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2960 4934 1316 Size: 1644 1974 4399 Fragments arranged by size: 4399 1974 1644 XbaI T'CTAG_A Cuts at: 5353 5353 Size: 8017 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2242 2497 3610 3832 2242 Size: 255 1113 222 6427 Fragments arranged by size: 6427 1113 255 222 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8017 XmaI C'CCGG_G Cuts at: 1870 2708 3105 3789 5342 1870 Size: 838 397 684 1553 4545 Fragments arranged by size: 4545 1553 838 684 397 XmnI GAAnn'nnTTC Cuts at: 3395 6146 3395 Size: 2751 5266 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5825 276 Size: 53 83 186 936 4291 2468 Fragments arranged by size: 4291 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoRV NruI NsiI PacI PspXI RsrII SwaI