Restriction map for
pFN21ASDA0876
(Linear) (Six-base) MAPSORT of: KIAA0876.seq Check: 9024 from: 1 to: 3288
KIAA0876.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 210 2713 3249 3288
Size: 210 2503 536 39
Fragments arranged by size:
2503 536 210 39
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 756 772 1114 3288
Size: 756 16 342 2174
Fragments arranged by size:
2174 756 342 16
AfeI AGC'GCT
Cuts at: 0 985 1196 3288
Size: 985 211 2092
Fragments arranged by size:
2092 985 211
AflIII A'CryG_T
Cuts at: 0 956 1631 2350 3288
Size: 956 675 719 938
Fragments arranged by size:
956 938 719 675
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2318 3288
Size: 2318 970
AlwNI CAG_nnn'CTG
Cuts at: 0 1281 1480 2029 3288
Size: 1281 199 549 1259
Fragments arranged by size:
1281 1259 549 199
ApaI G_GGCC'C
Cuts at: 0 1474 3288
Size: 1474 1814
ApaLI G'TGCA_C
Cuts at: 0 918 3288
Size: 918 2370
ApoI r'AATT_y
Cuts at: 0 70 799 3288
Size: 70 729 2489
Fragments arranged by size:
2489 729 70
AvaI C'yCGr_G
Cuts at: 0 685 979 1057 1082 1252 1766 2326 2473
Size: 685 294 78 25 170 514 560 147
Cuts at: 2473 2530 3150 3288
Size: 57 620 138
Fragments arranged by size:
685 620 560 514 294 170 147 138
78 57 25
AvrII C'CTAG_G
Cuts at: 0 2760 3288
Size: 2760 528
BamHI G'GATC_C
Cuts at: 0 764 2444 3288
Size: 764 1680 844
Fragments arranged by size:
1680 844 764
BanI G'GyrC_C
Cuts at: 0 19 207 332 496 1509 1536 1593 1785
Size: 19 188 125 164 1013 27 57 192
Cuts at: 1785 1832 2106 2590 2821 3129 3190 3288
Size: 47 274 484 231 308 61 98
Fragments arranged by size:
1013 484 308 274 231 192 188 164
125 98 61 57 47 27 19
BanII G_rGCy'C
Cuts at: 0 120 694 1069 1460 1474 1501 2045 2954
Size: 120 574 375 391 14 27 544 909
Cuts at: 2954 3143 3282 3288
Size: 189 139 6
Fragments arranged by size:
909 574 544 391 375 189 139 120
27 14 6
BbeI G_GCGC'C
Cuts at: 0 23 211 1836 3288
Size: 23 188 1625 1452
Fragments arranged by size:
1625 1452 188 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 553 1163 2173 3288
Size: 553 610 1010 1115
Fragments arranged by size:
1115 1010 610 553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 486 520 3288
Size: 486 34 2768
Fragments arranged by size:
2768 486 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2608 3288
Size: 2608 680
BclI T'GATC_A
Cuts at: 0 806 3288
Size: 806 2482
BglI GCCn_nnn'nGGC
Cuts at: 0 124 239 3288
Size: 124 115 3049
Fragments arranged by size:
3049 124 115
BglII A'GATC_T
Cuts at: 0 944 3288
Size: 944 2344
BlpI GC'TnA_GC
Cuts at: 0 1063 3288
Size: 1063 2225
Bme1580I G_kGCm'C
Cuts at: 0 922 1474 1529 1541 3132 3193 3288
Size: 922 552 55 12 1591 61 95
Fragments arranged by size:
1591 922 552 95 61 55 12
BmgBI CAC'GTC
Cuts at: 0 195 435 957 2351 2627 3288
Size: 195 240 522 1394 276 661
Fragments arranged by size:
1394 661 522 276 240 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 435 1535 3288
Size: 435 1100 1753
Fragments arranged by size:
1753 1100 435
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1083 2637 3288
Size: 1083 1554 651
Fragments arranged by size:
1554 1083 651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1092 1474 3097 3288
Size: 679 413 382 1623 191
Fragments arranged by size:
1623 679 413 382 191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1878 1974 2695 3288
Size: 1878 96 721 593
Fragments arranged by size:
1878 721 593 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 1121 1750 2700 3288
Size: 1121 629 950 588
Fragments arranged by size:
1121 950 629 588
BsaAI yAC'GTr
Cuts at: 0 93 923 1590 1632 2451 2637 3234 3288
Size: 93 830 667 42 819 186 597 54
Fragments arranged by size:
830 819 667 597 186 93 54 42
BsaBI GATnn'nnATC
Cuts at: 0 1816 3288
Size: 1816 1472
BsaHI Gr'CG_yC
Cuts at: 0 20 208 707 1833 1949 3288
Size: 20 188 499 1126 116 1339
Fragments arranged by size:
1339 1126 499 188 116 20
BsaWI w'CCGG_w
Cuts at: 0 461 1121 2954 3288
Size: 461 660 1833 334
Fragments arranged by size:
1833 660 461 334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 475 505 604 634 1420 1450 1618 1648
Size: 475 30 99 30 786 30 168 30
Cuts at: 1648 2848 2878 2941 2971 3288
Size: 1200 30 63 30 317
Fragments arranged by size:
1200 786 475 317 168 99 63 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1096 1219 1243 1270 1273 1276 1279 1282
Size: 1096 123 24 27 3 3 3 3
Cuts at: 1282 1441 1507 1561 1918 2423 2985 3094 3097
Size: 159 66 54 357 505 562 109 3
Cuts at: 3097 3172 3288
Size: 75 116
Fragments arranged by size:
1096 562 505 357 159 123 116 109
75 66 54 27 24 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 31 1186 1210 1234 1467 2919 3041 3288
Size: 31 1155 24 24 233 1452 122 247
Fragments arranged by size:
1452 1155 247 233 122 31 24 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1748 2043 3283 3288
Size: 586 1162 295 1240 5
Fragments arranged by size:
1240 1162 586 295 5
BsiEI CG_ry'CG
Cuts at: 0 1293 1603 1852 2176 3288
Size: 1293 310 249 324 1112
Fragments arranged by size:
1293 1112 324 310 249
BsiHKAI G_wGCw'C
Cuts at: 0 647 694 922 1069 1631 1658 2608 2618
Size: 647 47 228 147 562 27 950 10
Cuts at: 2618 2666 2954 3124 3288
Size: 48 288 170 164
Fragments arranged by size:
950 647 562 288 228 170 164 147
48 47 27 10
Bsp1286I G_dGCh'C
Cuts at: 0 120 647 694 922 1069 1460 1474 1501
Size: 120 527 47 228 147 391 14 27
Cuts at: 1501 1529 1541 1631 1658 2045 2608 2618 2666
Size: 28 12 90 27 387 563 10 48
Cuts at: 2666 2954 3124 3132 3143 3193 3282 3288
Size: 288 170 8 11 50 89 6
Fragments arranged by size:
563 527 391 387 288 228 170 147
120 90 89 50 48 47 28 27
27 14 12 11 10 8 6
BspEI T'CCGG_A
Cuts at: 0 461 3288
Size: 461 2827
BspHI T'CATG_A
Cuts at: 0 44 803 3288
Size: 44 759 2485
Fragments arranged by size:
2485 759 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 210 608 1103 1735 2286 2411 2650 2713
Size: 210 398 495 632 551 125 239 63
Cuts at: 2713 2845 3249 3288
Size: 132 404 39
Fragments arranged by size:
632 551 495 404 398 239 210 132
125 63 39
BsrBI CCG'CTC
Cuts at: 0 663 1359 2178 3104 3288
Size: 663 696 819 926 184
Fragments arranged by size:
926 819 696 663 184
BsrFI r'CCGG_y
Cuts at: 0 832 1506 1829 1847 3269 3288
Size: 832 674 323 18 1422 19
Fragments arranged by size:
1422 832 674 323 19 18
BsrGI T'GTAC_A
Cuts at: 0 584 3288
Size: 584 2704
BssHII G'CGCG_C
Cuts at: 0 2032 2479 3288
Size: 2032 447 809
Fragments arranged by size:
2032 809 447
BssSI C'ACGA_G
Cuts at: 0 2898 3288
Size: 2898 390
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1281 2029 2288 3288
Size: 1281 748 259 1000
Fragments arranged by size:
1281 1000 748 259
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 3288
Size: 282 3006
BstYI r'GATC_y
Cuts at: 0 764 944 2444 3288
Size: 764 180 1500 844
Fragments arranged by size:
1500 844 764 180
Bsu36I CC'TnA_GG
Cuts at: 0 1532 2746 3288
Size: 1532 1214 542
Fragments arranged by size:
1532 1214 542
BtgI C'CryG_G
Cuts at: 0 15 62 166 328 950 1040 1487 1597
Size: 15 47 104 162 622 90 447 110
Cuts at: 1597 1700 3203 3288
Size: 103 1503 85
Fragments arranged by size:
1503 622 447 162 110 104 103 90
85 47 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2448 3288
Size: 2448 840
BtsI GCAGTG_nn'
Cuts at: 0 1279 1743 1981 2616 3288
Size: 1279 464 238 635 672
Fragments arranged by size:
1279 672 635 464 238
DraI TTT'AAA
Cuts at: 0 75 3288
Size: 75 3213
DraIII CAC_nnn'GTG
Cuts at: 0 917 2511 3288
Size: 917 1594 777
Fragments arranged by size:
1594 917 777
DrdI GACnn_nn'nnGTC
Cuts at: 0 234 3288
Size: 234 3054
EaeI y'GGCC_r
Cuts at: 0 134 302 668 908 1290 1336 1600 1849
Size: 134 168 366 240 382 46 264 249
Cuts at: 1849 1991 2138 3288
Size: 142 147 1150
Fragments arranged by size:
1150 382 366 264 249 240 168 147
142 134 46
EagI C'GGCC_G
Cuts at: 0 1290 1600 1849 3288
Size: 1290 310 249 1439
Fragments arranged by size:
1439 1290 310 249
EarI CTCTTCn'nnn_
Cuts at: 0 250 1307 1363 1819 2071 3288
Size: 250 1057 56 456 252 1217
Fragments arranged by size:
1217 1057 456 252 250 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3234 3288
Size: 3234 54
EcoICRI GAG'CTC
Cuts at: 0 692 1067 2952 3288
Size: 692 375 1885 336
Fragments arranged by size:
1885 692 375 336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 756 772 1092 1114 1474 3097 3288
Size: 679 77 16 320 22 360 1623 191
Fragments arranged by size:
1623 679 360 320 191 77 22 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1204 2125 2764 2977 3079 3256 3288
Size: 1204 921 639 213 102 177 32
Fragments arranged by size:
1204 921 639 213 177 102 32
EcoO109I rG'GnC_Cy
Cuts at: 0 1011 1477 2085 2923 3038 3288
Size: 1011 466 608 838 115 250
Fragments arranged by size:
1011 838 608 466 250 115
EcoRI G'AATT_C
Cuts at: 0 799 3288
Size: 799 2489
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 570 1389 1632 3288
Size: 570 819 243 1656
Fragments arranged by size:
1656 819 570 243
FseI GG_CCGG'CC
Cuts at: 0 1851 3273 3288
Size: 1851 1422 15
Fragments arranged by size:
1851 1422 15
FspI TGC'GCA
Cuts at: 0 858 967 3288
Size: 858 109 2321
Fragments arranged by size:
2321 858 109
FspAI rTGC'GCAy
Cuts at: 0 967 3288
Size: 967 2321
HaeII r_GCGC'y
Cuts at: 0 23 211 657 987 1048 1198 1435 1836
Size: 23 188 446 330 61 150 237 401
Cuts at: 1836 2505 2525 3180 3288
Size: 669 20 655 108
Fragments arranged by size:
669 655 446 401 330 237 188 150
108 61 23 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 1618 1650 1894 1926 2165 2197
Size: 604 32 982 32 244 32 239 32
Cuts at: 2197 2848 2880 3022 3054 3288
Size: 651 32 142 32 234
Fragments arranged by size:
982 651 604 244 239 234 142 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1248 3288
Size: 1248 2040
KasI G'GCGC_C
Cuts at: 0 19 207 1832 3288
Size: 19 188 1625 1456
Fragments arranged by size:
1625 1456 188 19
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1699 2763 3288
Size: 1699 1064 525
Fragments arranged by size:
1699 1064 525
MscI TGG'CCA
Cuts at: 0 136 304 670 910 3288
Size: 136 168 366 240 2378
Fragments arranged by size:
2378 366 240 168 136
MslI CAynn'nnrTG
Cuts at: 0 280 955 1579 3288
Size: 280 675 624 1709
Fragments arranged by size:
1709 675 624 280
MspA1I CmG'CkG
Cuts at: 0 35 168 330 413 1395 1599 1962 2026
Size: 35 133 162 83 982 204 363 64
Cuts at: 2026 2226 2538 2807 2919 3045 3288
Size: 200 312 269 112 126 243
Fragments arranged by size:
982 363 312 269 243 204 200 162
133 126 112 83 64 35
NaeI GCC'GGC
Cuts at: 0 834 1508 1831 1849 3271 3288
Size: 834 674 323 18 1422 17
Fragments arranged by size:
1422 834 674 323 18 17
NarI GG'CG_CC
Cuts at: 0 20 208 1833 3288
Size: 20 188 1625 1455
Fragments arranged by size:
1625 1455 188 20
NcoI C'CATG_G
Cuts at: 0 62 950 1487 1700 3288
Size: 62 888 537 213 1588
Fragments arranged by size:
1588 888 537 213 62
NgoMIV G'CCGG_C
Cuts at: 0 832 1506 1829 1847 3269 3288
Size: 832 674 323 18 1422 19
Fragments arranged by size:
1422 832 674 323 19 18
NspI r_CATG'y
Cuts at: 0 543 3288
Size: 543 2745
PasI CC'CwG_GG
Cuts at: 0 1171 1842 3288
Size: 1171 671 1446
Fragments arranged by size:
1446 1171 671
PflMI CCAn_nnn'nTGG
Cuts at: 0 222 1842 2511 3288
Size: 222 1620 669 777
Fragments arranged by size:
1620 777 669 222
PfoI T'CCnGG_A
Cuts at: 0 470 1069 3288
Size: 470 599 2219
Fragments arranged by size:
2219 599 470
PmlI CAC'GTG
Cuts at: 0 923 1632 2451 2637 3288
Size: 923 709 819 186 651
Fragments arranged by size:
923 819 709 651 186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1999 2031 3288
Size: 1999 32 1257
Fragments arranged by size:
1999 1257 32
PpuMI rG'GwC_Cy
Cuts at: 0 1011 2923 3038 3288
Size: 1011 1912 115 250
Fragments arranged by size:
1912 1011 250 115
PspOMI G'GGCC_C
Cuts at: 0 1470 3288
Size: 1470 1818
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 508 540 3288
Size: 508 32 2748
Fragments arranged by size:
2748 508 32
PstI C_TGCA'G
Cuts at: 0 977 1485 1976 2297 2423 3260 3288
Size: 977 508 491 321 126 837 28
Fragments arranged by size:
977 837 508 491 321 126 28
PvuII CAG'CTG
Cuts at: 0 35 1395 2919 3045 3288
Size: 35 1360 1524 126 243
Fragments arranged by size:
1524 1360 243 126 35
SacI G_AGCT'C
Cuts at: 0 694 1069 2954 3288
Size: 694 375 1885 334
Fragments arranged by size:
1885 694 375 334
SacII CC_GC'GG
Cuts at: 0 169 331 1600 3288
Size: 169 162 1269 1688
Fragments arranged by size:
1688 1269 169 162
SanDI GG'GwC_CC
Cuts at: 0 2923 3038 3288
Size: 2923 115 250
Fragments arranged by size:
2923 250 115
SapI GCTCTTCn'nnn_
Cuts at: 0 1363 1819 2071 3288
Size: 1363 456 252 1217
Fragments arranged by size:
1363 1217 456 252
SbfI CC_TGCA'GG
Cuts at: 0 2297 3260 3288
Size: 2297 963 28
Fragments arranged by size:
2297 963 28
ScaI AGT'ACT
Cuts at: 0 319 3288
Size: 319 2969
SexAI A'CCwGG_T
Cuts at: 0 3017 3288
Size: 3017 271
SfcI C'TryA_G
Cuts at: 0 973 1481 1972 2089 2293 2419 2868 3256
Size: 973 508 491 117 204 126 449 388
Cuts at: 3256 3288
Size: 32
Fragments arranged by size:
973 508 491 449 388 204 126 117
32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 239 3288
Size: 239 3049
SfoI GGC'GCC
Cuts at: 0 21 209 1834 3288
Size: 21 188 1625 1454
Fragments arranged by size:
1625 1454 188 21
SmaI CCC'GGG
Cuts at: 0 687 1084 1768 3288
Size: 687 397 684 1520
Fragments arranged by size:
1520 687 684 397
SmlI C'TyrA_G
Cuts at: 0 1893 1953 2710 3288
Size: 1893 60 757 578
Fragments arranged by size:
1893 757 578 60
SrfI GCCC'GGGC
Cuts at: 0 1768 3288
Size: 1768 1520
StuI AGG'CCT
Cuts at: 0 1648 1891 3288
Size: 1648 243 1397
Fragments arranged by size:
1648 1397 243
StyI C'CwwG_G
Cuts at: 0 62 950 1343 1487 1700 2760 3164 3288
Size: 62 888 393 144 213 1060 404 124
Fragments arranged by size:
1060 888 404 393 213 144 124 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2124 3288
Size: 2124 1164
TatI w'GTAC_w
Cuts at: 0 254 317 530 584 2558 2798 3288
Size: 254 63 213 54 1974 240 490
Fragments arranged by size:
1974 490 254 240 213 63 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 1420 1452 2941 2973 3288
Size: 475 32 913 32 1489 32 315
Fragments arranged by size:
1489 913 475 315 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 937 2911 3288
Size: 937 1974 377
Fragments arranged by size:
1974 937 377
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 474 1587 1809 3288
Size: 219 255 1113 222 1479
Fragments arranged by size:
1479 1113 255 222 219
XmaI C'CCGG_G
Cuts at: 0 685 1082 1766 3288
Size: 685 397 684 1522
Fragments arranged by size:
1522 685 684 397
XmnI GAAnn'nnTTC
Cuts at: 0 1372 3288
Size: 1372 1916
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AlwNI ApaI ApaLI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PsrI PstI PvuII SacI SacII
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AloI AscI AseI AsiSI BaeI BbvCI BfrBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrDI BstBI BstEII BstZ17I
ClaI CspCI EcoRV HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PshAI PsiI PspXI PvuI RsrII SalI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI XbaI XhoI ZraI