Restriction map for
pFN21ASDA0848
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI AlwNI Aor51HI AscI
Asp718I AspEI BamHI BbeI BbvCI
BcgI BciVI BclI BglI BglII
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspMI BsrBI BsrFI BssHII Bst1107I
BstBI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FauI
FbaI FseI HaeII HpaI KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SacII SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 2168 GTCGAC GT^[AC][GT]AC
AflII 370 CTTAAG C^TTAAG
AflIII 709 ACATGT A^C[GA][CT]GT
Alw44I 2743 GTGCAC G^TGCAC
AocI 1599 CCTGAGG CC^T[ACGT]AGG
ApaLI 2743 GTGCAC G^TGCAC
AseI 316 ATTAAT AT^TAAT
AsnI 316 ATTAAT AT^TAAT
AspI 1705 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2860 CTCGAG C^[CT]CG[GA]G
AviII 1805 TGCGCA TGC^GCA
AvrII 2373 CCTAGG C^CTAGG
BalI 916 TGGCCA TGG^CCA
BanI 1902 GGCACC G^G[CT][GA]CC
BbrPI 2740 CACGTG CAC^GTG
BfrI 370 CTTAAG C^TTAAG
BlnI 2373 CCTAGG C^CTAGG
BsaI 784 GAGACC GGTCTC(1/5)
BsaWI 2423 ACCGGA [AT]^CCGG[AT]
BsoBI 2860 CTCGAG C^[CT]CG[GA]G
BspLU11I 709 ACATGT A^CATGT
BssSI 2273 CTCGTG CACGAG(-5/-1)
BstAPI 2314 GCAGGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2238 GGTTACC G^GT[ACGT]ACC
BstPI 2238 GGTTACC G^GT[ACGT]ACC
BstXI 795 CCACTTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1302 GGATCT [GA]^GATC[CT]
Bsu36I 1599 CCTGAGG CC^T[ACGT]AGG
CfoI 1806 GCGC GCG^C
DraIII 1122 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 916 TGGCCA [CT]^GGCC[GA]
Eco81I 1599 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2238 GGTTACC G^GT[ACGT]ACC
FspI 1805 TGCGCA TGC^GCA
HhaI 1806 GCGC GCG^C
HinP1I 1806 GCGC G^CGC
HindIII 1046 AAGCTT A^AGCTT
MfeI 65 CAATTG C^AATTG
MflI 1302 GGATCT [GA]^GATC[CT]
MluNI 916 TGGCCA TGG^CCA
MscI 916 TGGCCA TGG^CCA
MunI 65 CAATTG C^AATTG
NcoI 801 CCATGG C^CATGG
PaeR7I 2860 CTCGAG C^TCGAG
PflFI 1705 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2521 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2740 CACGTG CAC^GTG
PmlI 2740 CACGTG CAC^GTG
PpuMI 809 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 316 ATTAAT AT^TAAT
SacI 676 GAGCTC GAGCT^C
SalI 2168 GTCGAC G^TCGAC
SexAI 1706 ACCTGGT A^CC[AT]GGT
SphI 2756 GCATGC GCATG^C
SspI 202 AATATT AAT^ATT
Tth111I 1705 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2521 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 450 TCTAGA T^CTAGA
XhoI 2860 CTCGAG C^TCGAG
XhoII 1302 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 1564 GACGCC G[GA]^CG[CT]C
2042 GGCGTC
ApaI 1944 GGGCCC GGGCC^C
2453 GGGCCC
BsaHI 1564 GACGCC G[GA]^CG[CT]C
2042 GGCGTC
Bsp1407I 706 TGTACA T^GTACA
720 TGTACA
BsrGI 706 TGTACA T^GTACA
720 TGTACA
EcoNI 96 CCTAATAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2620 CCTGGGGGAGG
EcoRI 258 GAATTC G^AATTC
881 GAATTC
EcoT22I 332 ATGCAT ATGCA^T
911 ATGCAT
HgaI 1564 GACGC GACGC(5/10)
2043 GCGTC
Hin1I 1564 GACGCC G[GA]^CG[CT]C
2042 GGCGTC
HincII 1168 GTCAAC GT[CT]^[GA]AC
2168 GTCGAC
HindII 1168 GTCAAC GT[CT]^[GA]AC
2168 GTCGAC
MspA1I 682 CAGCTG C[AC]G^C[GT]G
1856 CAGCTG
NsiI 332 ATGCAT ATGCA^T
911 ATGCAT
PvuII 682 CAGCTG CAG^CTG
1856 CAGCTG
SmlI 370 CTTAAG C^T[CT][GA]AG
2860 CTCGAG
SspBI 706 TGTACA T^GTACA
720 TGTACA
StuI 1431 AGGCCT AGG^CCT
2016 AGGCCT
XcmI 2517 CCACCCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2586 CCATGAGAAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI