(Circular) (Six-base) MAPSORT of: pFN21ASDA0848.seq Check: 899 from: 1 to: 7660 pFN21ASDA0848 7660 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5470 278 Size: 53 83 186 936 3934 2468 Fragments arranged by size: 3934 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4192 5003 1937 Size: 2255 811 4594 Fragments arranged by size: 4594 2255 811 Acc65I G'GTAC_C Cuts at: 4981 4981 Size: 7660 AclI AA'CG_TT Cuts at: 1563 5787 6160 1563 Size: 4224 373 3063 Fragments arranged by size: 4224 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2425 2771 3505 4069 5723 7107 7375 1632 Size: 793 346 734 564 1654 1384 268 1917 Fragments arranged by size: 1917 1654 1384 793 734 564 346 268 AflII C'TTAA_G Cuts at: 820 1017 2393 820 Size: 197 1376 6087 Fragments arranged by size: 6087 1376 197 AflIII A'CryG_T Cuts at: 1203 2732 6559 7536 1203 Size: 1529 3827 977 1327 Fragments arranged by size: 3827 1529 1327 977 AgeI A'CCGG_T Cuts at: 6491 6491 Size: 7660 AhdI GACnn_n'nnGTC Cuts at: 6389 6389 Size: 7660 AleI CACnn'nnGTG Cuts at: 1166 4511 1166 Size: 3345 4315 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4145 1184 Size: 2961 4699 AlwNI CAG_nnn'CTG Cuts at: 5403 6975 5403 Size: 1572 6088 ApaI G_GGCC'C Cuts at: 3971 4480 3971 Size: 509 7151 ApaLI G'TGCA_C Cuts at: 4766 5717 6873 4766 Size: 951 1156 5553 Fragments arranged by size: 5553 1156 951 ApoI r'AATT_y Cuts at: 1493 2281 2904 3310 4969 5195 5347 7456 1493 Size: 788 623 406 1659 226 152 2109 1697 Fragments arranged by size: 2109 1697 1659 788 623 406 226 152 AseI AT'TA_AT Cuts at: 160 1032 2340 6214 160 Size: 872 1308 3874 1606 Fragments arranged by size: 3874 1606 1308 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7660 AvaI C'yCGr_G Cuts at: 1870 1943 4883 4985 1870 Size: 73 2940 102 4545 Fragments arranged by size: 4545 2940 102 73 AvrII C'CTAG_G Cuts at: 4396 4396 Size: 7660 BamHI G'GATC_C Cuts at: 4990 5357 4990 Size: 367 7293 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3925 4981 6436 618 Size: 325 217 627 2138 1056 1455 1842 Fragments arranged by size: 2138 1842 1455 1056 627 325 217 BanII G_rGCy'C Cuts at: 1391 2151 2703 3803 3971 4480 4979 1391 Size: 760 552 1100 168 509 499 4072 Fragments arranged by size: 4072 1100 760 552 509 499 168 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2431 2858 4943 928 Size: 971 532 427 2085 3645 Fragments arranged by size: 3645 2085 971 532 427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5851 5885 1903 Size: 34 3914 34 3678 Fragments arranged by size: 3914 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5552 6762 5552 Size: 1210 6450 BclI T'GATC_A Cuts at: 1548 1548 Size: 7660 BfrBI ATG'CAT Cuts at: 2357 2936 2357 Size: 579 7081 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6271 136 Size: 107 122 71 631 605 4599 1525 Fragments arranged by size: 4599 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7655 7655 Size: 7660 BlpI GC'TnA_GC Cuts at: 5077 5077 Size: 7660 Bme1580I G_kGCm'C Cuts at: 1790 3928 3971 4480 4746 4770 5721 6877 1790 Size: 2138 43 509 266 24 951 1156 2573 Fragments arranged by size: 2573 2138 1156 951 509 266 43 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7660 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3156 4590 5391 Size: 416 53 83 399 24 1739 1434 801 Cuts at: 5391 6349 442 Size: 958 1753 Fragments arranged by size: 1753 1739 1434 958 801 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7660 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2397 2687 3903 3924 4194 4952 6320 1239 Size: 1158 290 1216 21 270 758 1368 2579 Fragments arranged by size: 2579 1368 1216 1158 758 290 270 21 Bpu10I CC'TnA_GC Cuts at: 3021 3021 Size: 7660 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5785 6650 6948 5785 Size: 865 298 6497 Fragments arranged by size: 6497 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2801 6323 882 Size: 617 1302 3522 2219 Fragments arranged by size: 3522 2219 1302 617 BsaAI yAC'GTr Cuts at: 493 1204 2633 4054 4765 4957 5451 493 Size: 711 1429 1421 711 192 494 2702 Fragments arranged by size: 2702 1429 1421 711 711 494 192 BsaBI GATnn'nnATC Cuts at: 7654 7654 Size: 7660 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3588 4066 5467 5849 Size: 53 83 186 936 2055 478 1401 382 Cuts at: 5849 6484 275 Size: 635 1451 Fragments arranged by size: 2055 1451 1401 936 635 478 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4446 6093 6491 6765 6912 7323 4446 Size: 1647 398 274 147 411 4783 Fragments arranged by size: 4783 1647 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7435 Fragments arranged by size: 7435 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3948 4316 4319 4322 1186 Size: 2762 368 3 3 4524 Fragments arranged by size: 4524 2762 368 3 3 BseYI C'CCAG_C Cuts at: 3892 3897 4497 4543 6863 7359 3892 Size: 5 600 46 2320 496 4193 Fragments arranged by size: 4193 2320 600 496 46 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3703 3851 4365 4690 4745 1163 Size: 573 1967 148 514 325 55 4078 Fragments arranged by size: 4078 1967 573 514 325 148 55 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5110 5871 6899 1540 Size: 275 186 3109 761 1028 2301 Fragments arranged by size: 3109 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2703 4059 4770 5721 5806 6877 2703 Size: 1356 711 951 85 1071 3486 Fragments arranged by size: 3486 1356 1071 951 711 85 BsmI GAATG_Cn' Cuts at: 2673 2769 2938 3253 3682 5171 5264 2673 Size: 96 169 315 429 1489 93 5069 Fragments arranged by size: 5069 1489 429 315 169 96 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2703 3803 3928 3971 4059 4480 Size: 399 361 552 1100 125 43 88 421 Cuts at: 4480 4746 4770 4979 5721 5806 6877 1391 Size: 266 24 209 742 85 1071 2174 Fragments arranged by size: 2174 1100 1071 742 552 421 399 361 266 209 125 88 85 43 24 BspEI T'CCGG_A Cuts at: 7323 7323 Size: 7660 BspHI T'CATG_A Cuts at: 5549 7279 5549 Size: 1730 5930 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5015 844 Size: 4171 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5547 1394 Size: 429 3724 3507 Fragments arranged by size: 3724 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2357 3099 4139 6155 6329 65 Size: 1183 1109 742 1040 2016 174 1396 Fragments arranged by size: 2016 1396 1183 1109 1040 742 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6304 6491 1700 Size: 110 4494 187 2869 Fragments arranged by size: 4494 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2729 2743 96 Size: 2633 14 5013 Fragments arranged by size: 5013 2633 14 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7660 BssSI C'ACGA_G Cuts at: 4296 5720 6732 4296 Size: 1424 1012 5224 Fragments arranged by size: 5224 1424 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4343 794 Size: 3549 4111 BstBI TT'CG_AA Cuts at: 2007 6464 2007 Size: 4457 3203 BstEII G'GTnAC_C Cuts at: 1184 4261 1184 Size: 3077 4583 BstXI CCAn_nnnn'nTGG Cuts at: 2825 2825 Size: 7660 BstYI r'GATC_y Cuts at: 1972 3325 4990 5357 5742 5759 7211 7297 7309 Size: 1353 1665 367 385 17 1452 86 12 Cuts at: 7309 7655 1972 Size: 346 1977 Fragments arranged by size: 1977 1665 1452 1353 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3623 3623 Size: 7660 BtgI C'CryG_G Cuts at: 513 1065 2824 3651 4227 513 Size: 552 1759 827 576 3946 Fragments arranged by size: 3946 1759 827 576 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6921 Fragments arranged by size: 6921 683 56 BtsI GCAGTG_nn' Cuts at: 4491 5176 5970 5990 4491 Size: 685 794 20 6161 Fragments arranged by size: 6161 794 685 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7660 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7625 DraI TTT'AAA Cuts at: 2263 2403 2562 3742 4964 2263 Size: 140 159 1180 1222 4959 Fragments arranged by size: 4959 1222 1180 159 140 DraIII CAC_nnn'GTG Cuts at: 3150 3150 Size: 7660 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6667 809 Size: 507 5351 1802 Fragments arranged by size: 5351 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2939 5107 5996 Size: 54 1425 50 275 18 1109 2168 889 Cuts at: 5996 7625 8 Size: 1629 43 Fragments arranged by size: 2168 1629 1425 1109 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5107 1537 Size: 275 3295 4090 Fragments arranged by size: 4090 3295 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2747 3410 4835 5590 1353 Size: 1394 663 1425 755 3423 Fragments arranged by size: 3423 1425 1394 755 663 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4188 4592 6247 6621 6767 387 Size: 1064 138 2599 404 1655 374 146 1280 Fragments arranged by size: 2599 1655 1280 1064 404 374 146 138 EcoICRI GAG'CTC Cuts at: 2701 2701 Size: 7660 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2397 2425 2687 2771 3505 3903 3924 Size: 393 765 28 262 84 734 398 21 Cuts at: 3924 4069 4194 4952 5723 6320 7107 7375 1239 Size: 145 125 758 771 597 787 268 1524 Fragments arranged by size: 1524 787 771 765 758 734 597 398 393 268 262 145 125 84 28 21 EcoNI CCTnn'n_nnAGG Cuts at: 2123 4647 2123 Size: 2524 5136 EcoO109I rG'GnC_Cy Cuts at: 2833 3240 3372 3967 3968 4219 4477 2833 Size: 407 132 595 1 251 258 6016 Fragments arranged by size: 6016 595 407 258 251 132 1 EcoRI G'AATT_C Cuts at: 2281 2904 4969 5347 2281 Size: 623 2065 378 4594 Fragments arranged by size: 4594 2065 623 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3421 3652 4099 4841 3421 Size: 231 447 742 6240 Fragments arranged by size: 6240 742 447 231 FspI TGC'GCA Cuts at: 3830 6166 3830 Size: 2336 5324 FspAI rTGC'GCAy Cuts at: 3830 3830 Size: 7660 HaeII r_GCGC'y Cuts at: 1678 6807 1678 Size: 5129 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2234 2266 2398 2430 3111 Size: 32 135 32 662 32 132 32 681 Cuts at: 3111 3143 3259 3291 4507 4539 6301 6333 6375 Size: 32 116 32 1216 32 1762 32 42 Cuts at: 6375 6407 1373 Size: 32 2626 Fragments arranged by size: 2626 1762 1216 681 662 135 132 116 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3193 4193 5004 5250 669 Size: 988 281 1255 1000 811 246 3079 Fragments arranged by size: 3079 1255 1000 988 811 281 246 HindIII A'AGCT_T Cuts at: 748 3069 748 Size: 2321 5339 HpaI GTT'AAC Cuts at: 5250 5250 Size: 7660 KpnI G_GTAC'C Cuts at: 4985 4985 Size: 7660 MfeI C'AATT_G Cuts at: 2088 5259 2088 Size: 3171 4489 MluI A'CGCG_T Cuts at: 7536 7536 Size: 7660 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2612 6774 6958 1305 Size: 1307 4162 184 2007 Fragments arranged by size: 4162 2007 1307 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2941 10 Size: 54 1425 343 1109 4729 Fragments arranged by size: 4729 1425 1109 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2579 2823 3171 3675 3822 4127 4239 Size: 648 1413 244 348 504 147 305 112 Cuts at: 4239 4511 5618 5977 6136 518 Size: 272 1107 359 159 2042 Fragments arranged by size: 2042 1413 1107 648 504 359 348 305 272 244 159 147 112 MspA1I CmG'CkG Cuts at: 1606 2707 3881 5076 5753 6901 7146 1606 Size: 1101 1174 1195 677 1148 245 2120 Fragments arranged by size: 2120 1195 1174 1148 1101 677 245 NcoI C'CATG_G Cuts at: 513 1065 2824 513 Size: 552 1759 5349 Fragments arranged by size: 5349 1759 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7660 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7660 NotI GC'GGCC_GC Cuts at: 5107 5107 Size: 7660 NsiI A_TGCA'T Cuts at: 2359 2938 2359 Size: 579 7081 NspI r_CATG'y Cuts at: 1132 1229 2736 3448 4783 5018 6563 1132 Size: 97 1507 712 1335 235 1545 2229 Fragments arranged by size: 2229 1545 1507 1335 712 235 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7660 PciI A'CATG_T Cuts at: 2732 6559 2732 Size: 3827 3833 PflMI CCAn_nnn'nTGG Cuts at: 1108 4550 1108 Size: 3442 4218 PfoI T'CCnGG_A Cuts at: 3498 3663 4929 3498 Size: 165 1266 6229 Fragments arranged by size: 6229 1266 165 PmeI GTTT'AAAC Cuts at: 4964 4964 Size: 7660 PmlI CAC'GTG Cuts at: 4765 4765 Size: 7660 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5729 5761 7242 7274 5729 Size: 32 1481 32 6115 Fragments arranged by size: 6115 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2833 2833 Size: 7660 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7660 PsiI TTA'TAA Cuts at: 5230 5230 Size: 7660 PspOMI G'GGCC_C Cuts at: 3967 4476 3967 Size: 509 7151 PspXI vC'TCGA_Gb Cuts at: 4883 4883 Size: 7660 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3470 3502 3470 Size: 32 7628 PstI C_TGCA'G Cuts at: 830 2277 2487 3517 4039 5012 830 Size: 1447 210 1030 522 973 3478 Fragments arranged by size: 3478 1447 1030 973 522 210 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7660 PvuII CAG'CTG Cuts at: 2707 3881 2707 Size: 1174 6486 SacI G_AGCT'C Cuts at: 2703 2703 Size: 7660 SalI G'TCGA_C Cuts at: 1936 4191 5002 1936 Size: 2255 811 4594 Fragments arranged by size: 4594 2255 811 SbfI CC_TGCA'GG Cuts at: 5012 5012 Size: 7660 ScaI AGT'ACT Cuts at: 5908 7620 5908 Size: 1712 5948 SexAI A'CCwGG_T Cuts at: 3729 3729 Size: 7660 SfcI C'TryA_G Cuts at: 826 1045 2273 2483 3458 3513 4035 5008 6143 Size: 219 1228 210 975 55 522 973 1135 Cuts at: 6143 6824 7015 826 Size: 681 191 1471 Fragments arranged by size: 1471 1228 1135 975 973 681 522 219 210 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7660 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7660 SmaI CCC'GGG Cuts at: 1872 4987 1872 Size: 3115 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2393 4883 5764 6665 6927 820 Size: 197 926 450 2490 881 901 262 1553 Fragments arranged by size: 2490 1553 926 901 881 450 262 197 SnaBI TAC'GTA Cuts at: 493 4957 5451 493 Size: 4464 494 2702 Fragments arranged by size: 4464 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7660 SphI G_CATG'C Cuts at: 1132 4783 5018 1132 Size: 3651 235 3774 Fragments arranged by size: 3774 3651 235 SspI AAT'ATT Cuts at: 5 52 2227 5584 5 Size: 47 2175 3357 2081 Fragments arranged by size: 3357 2175 2081 47 StuI AGG'CCT Cuts at: 1517 3456 4041 1517 Size: 1939 585 5136 Fragments arranged by size: 5136 1939 585 StyI C'CwwG_G Cuts at: 513 1065 2458 2720 2824 3047 3178 3236 3321 Size: 552 1393 262 104 223 131 58 85 Cuts at: 3321 3710 4396 4425 5099 513 Size: 389 686 29 674 3074 Fragments arranged by size: 3074 1393 686 674 552 389 262 223 131 104 85 58 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3138 4373 4438 4557 5687 5704 5857 Size: 1255 1558 1235 65 119 1130 17 153 Cuts at: 5857 6042 6487 325 Size: 185 445 1498 Fragments arranged by size: 1558 1498 1255 1235 1130 445 185 153 119 65 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2729 2743 3477 Size: 274 80 33 51 1443 752 14 734 Cuts at: 3477 4245 5906 7618 96 Size: 768 1661 1712 138 Fragments arranged by size: 1712 1661 1443 768 752 734 274 138 80 51 33 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7620 7652 7620 Size: 32 7628 Tth111I GACn'n_nGTC Cuts at: 1316 3731 1316 Size: 2415 5245 XbaI T'CTAG_A Cuts at: 2473 4996 2473 Size: 2523 5137 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4547 4616 4547 Size: 69 7591 XhoI C'TCGA_G Cuts at: 1943 4883 1943 Size: 2940 4720 XmaI C'CCGG_G Cuts at: 1870 4985 1870 Size: 3115 4545 XmnI GAAnn'nnTTC Cuts at: 2769 3231 3635 4824 5789 2769 Size: 462 404 1189 965 4640 Fragments arranged by size: 4640 1189 965 462 404 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5468 276 Size: 53 83 186 936 3934 2468 Fragments arranged by size: 3934 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I EcoRV FseI KasI NaeI NarI NgoMIV NruI PacI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI