Restriction map for
pFN21ASDA0848
(Linear) (Six-base) MAPSORT of: KIAA0848.seq Check: 9361 from: 1 to: 2931
KIAA0848.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 2169 2931
Size: 2169 762
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 748 1482 2046 2931
Size: 402 346 734 564 885
Fragments arranged by size:
885 734 564 402 346
AflII C'TTAA_G
Cuts at: 0 370 2931
Size: 370 2561
AflIII A'CryG_T
Cuts at: 0 709 2931
Size: 709 2222
AleI CACnn'nnGTG
Cuts at: 0 2488 2931
Size: 2488 443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2122 2931
Size: 2122 809
ApaI G_GGCC'C
Cuts at: 0 1948 2457 2931
Size: 1948 509 474
Fragments arranged by size:
1948 509 474
ApaLI G'TGCA_C
Cuts at: 0 2743 2931
Size: 2743 188
ApoI r'AATT_y
Cuts at: 0 258 881 1287 2931
Size: 258 623 406 1644
Fragments arranged by size:
1644 623 406 258
AseI AT'TA_AT
Cuts at: 0 317 2931
Size: 317 2614
AvaI C'yCGr_G
Cuts at: 0 2860 2931
Size: 2860 71
AvrII C'CTAG_G
Cuts at: 0 2373 2931
Size: 2373 558
BanI G'GyrC_C
Cuts at: 0 1902 2931
Size: 1902 1029
BanII G_rGCy'C
Cuts at: 0 128 680 1780 1948 2457 2931
Size: 128 552 1100 168 509 474
Fragments arranged by size:
1100 552 509 474 168 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 408 835 2920 2931
Size: 408 427 2085 11
Fragments arranged by size:
2085 427 408 11
BfrBI ATG'CAT
Cuts at: 0 334 913 2931
Size: 334 579 2018
Fragments arranged by size:
2018 579 334
Bme1580I G_kGCm'C
Cuts at: 0 1905 1948 2457 2723 2747 2931
Size: 1905 43 509 266 24 184
Fragments arranged by size:
1905 509 266 184 43 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1133 2567 2931
Size: 1133 1434 364
Fragments arranged by size:
1434 1133 364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 664 1880 1901 2171 2929 2931
Size: 374 290 1216 21 270 758 2
Fragments arranged by size:
1216 758 374 290 270 21 2
Bpu10I CC'TnA_GC
Cuts at: 0 998 2931
Size: 998 1933
BsaI GGTCTCn'nnnn_
Cuts at: 0 778 2931
Size: 778 2153
BsaAI yAC'GTr
Cuts at: 0 610 2031 2742 2931
Size: 610 1421 711 189
Fragments arranged by size:
1421 711 610 189
BsaHI Gr'CG_yC
Cuts at: 0 1565 2043 2931
Size: 1565 478 888
Fragments arranged by size:
1565 888 478
BsaWI w'CCGG_w
Cuts at: 0 2423 2931
Size: 2423 508
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1925 2293 2296 2299 2931
Size: 1925 368 3 3 632
Fragments arranged by size:
1925 632 368 3 3
BseYI C'CCAG_C
Cuts at: 0 1869 1874 2474 2520 2931
Size: 1869 5 600 46 411
Fragments arranged by size:
1869 600 411 46 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1680 1828 2342 2667 2722 2931
Size: 1680 148 514 325 55 209
Fragments arranged by size:
1680 514 325 209 148 55
BsiHKAI G_wGCw'C
Cuts at: 0 680 2036 2747 2931
Size: 680 1356 711 184
Fragments arranged by size:
1356 711 680 184
BsmI GAATG_Cn'
Cuts at: 0 650 746 915 1230 1659 2931
Size: 650 96 169 315 429 1272
Fragments arranged by size:
1272 650 429 315 169 96
Bsp1286I G_dGCh'C
Cuts at: 0 128 680 1780 1905 1948 2036 2457 2723
Size: 128 552 1100 125 43 88 421 266
Cuts at: 2723 2747 2931
Size: 24 184
Fragments arranged by size:
1100 552 421 266 184 128 125 88
43 24
BsrDI GCAATG_nn'
Cuts at: 0 334 1076 2116 2931
Size: 334 742 1040 815
Fragments arranged by size:
1040 815 742 334
BsrGI T'GTAC_A
Cuts at: 0 706 720 2931
Size: 706 14 2211
Fragments arranged by size:
2211 706 14
BssSI C'ACGA_G
Cuts at: 0 2273 2931
Size: 2273 658
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2320 2931
Size: 2320 611
BstEII G'GTnAC_C
Cuts at: 0 2238 2931
Size: 2238 693
BstXI CCAn_nnnn'nTGG
Cuts at: 0 802 2931
Size: 802 2129
BstYI r'GATC_y
Cuts at: 0 1302 2931
Size: 1302 1629
Bsu36I CC'TnA_GG
Cuts at: 0 1600 2931
Size: 1600 1331
BtgI C'CryG_G
Cuts at: 0 801 1628 2204 2931
Size: 801 827 576 727
Fragments arranged by size:
827 801 727 576
BtsI GCAGTG_nn'
Cuts at: 0 2468 2931
Size: 2468 463
DraI TTT'AAA
Cuts at: 0 240 380 539 1719 2931
Size: 240 140 159 1180 1212
Fragments arranged by size:
1212 1180 240 159 140
DraIII CAC_nnn'GTG
Cuts at: 0 1127 2931
Size: 1127 1804
EaeI y'GGCC_r
Cuts at: 0 916 2931
Size: 916 2015
EarI CTCTTCn'nnn_
Cuts at: 0 724 1387 2812 2931
Size: 724 663 1425 119
Fragments arranged by size:
1425 724 663 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2165 2569 2931
Size: 2165 404 362
Fragments arranged by size:
2165 404 362
EcoICRI GAG'CTC
Cuts at: 0 678 2931
Size: 678 2253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 402 664 748 1482 1880 1901 2046
Size: 374 28 262 84 734 398 21 145
Cuts at: 2046 2171 2929 2931
Size: 125 758 2
Fragments arranged by size:
758 734 398 374 262 145 125 84
28 21 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 100 2624 2931
Size: 100 2524 307
Fragments arranged by size:
2524 307 100
EcoO109I rG'GnC_Cy
Cuts at: 0 810 1217 1349 1944 1945 2196 2454 2931
Size: 810 407 132 595 1 251 258 477
Fragments arranged by size:
810 595 477 407 258 251 132 1
EcoRI G'AATT_C
Cuts at: 0 258 881 2931
Size: 258 623 2050
Fragments arranged by size:
2050 623 258
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1398 1629 2076 2818 2931
Size: 1398 231 447 742 113
Fragments arranged by size:
1398 742 447 231 113
FspI TGC'GCA
Cuts at: 0 1807 2931
Size: 1807 1124
FspAI rTGC'GCAy
Cuts at: 0 1807 2931
Size: 1807 1124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 211 243 375 407 1088 1120 1236 1268
Size: 211 32 132 32 681 32 116 32
Cuts at: 1268 2484 2516 2931
Size: 1216 32 415
Fragments arranged by size:
1216 681 415 211 132 116 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1170 2170 2931
Size: 1170 1000 761
Fragments arranged by size:
1170 1000 761
HindIII A'AGCT_T
Cuts at: 0 1046 2931
Size: 1046 1885
MfeI C'AATT_G
Cuts at: 0 65 2931
Size: 65 2866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 2931
Size: 589 2342
MscI TGG'CCA
Cuts at: 0 918 2931
Size: 918 2013
MslI CAynn'nnrTG
Cuts at: 0 556 800 1148 1652 1799 2104 2216 2488
Size: 556 244 348 504 147 305 112 272
Cuts at: 2488 2931
Size: 443
Fragments arranged by size:
556 504 443 348 305 272 244 147
112
MspA1I CmG'CkG
Cuts at: 0 684 1858 2931
Size: 684 1174 1073
Fragments arranged by size:
1174 1073 684
NcoI C'CATG_G
Cuts at: 0 801 2931
Size: 801 2130
NsiI A_TGCA'T
Cuts at: 0 336 915 2931
Size: 336 579 2016
Fragments arranged by size:
2016 579 336
NspI r_CATG'y
Cuts at: 0 713 1425 2760 2931
Size: 713 712 1335 171
Fragments arranged by size:
1335 713 712 171
PciI A'CATG_T
Cuts at: 0 709 2931
Size: 709 2222
PflMI CCAn_nnn'nTGG
Cuts at: 0 2527 2931
Size: 2527 404
PfoI T'CCnGG_A
Cuts at: 0 1475 1640 2906 2931
Size: 1475 165 1266 25
Fragments arranged by size:
1475 1266 165 25
PmlI CAC'GTG
Cuts at: 0 2742 2931
Size: 2742 189
PpuMI rG'GwC_Cy
Cuts at: 0 810 2931
Size: 810 2121
PspOMI G'GGCC_C
Cuts at: 0 1944 2453 2931
Size: 1944 509 478
Fragments arranged by size:
1944 509 478
PspXI vC'TCGA_Gb
Cuts at: 0 2860 2931
Size: 2860 71
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1447 1479 2931
Size: 1447 32 1452
Fragments arranged by size:
1452 1447 32
PstI C_TGCA'G
Cuts at: 0 254 464 1494 2016 2931
Size: 254 210 1030 522 915
Fragments arranged by size:
1030 915 522 254 210
PvuII CAG'CTG
Cuts at: 0 684 1858 2931
Size: 684 1174 1073
Fragments arranged by size:
1174 1073 684
SacI G_AGCT'C
Cuts at: 0 680 2931
Size: 680 2251
SalI G'TCGA_C
Cuts at: 0 2168 2931
Size: 2168 763
SexAI A'CCwGG_T
Cuts at: 0 1706 2931
Size: 1706 1225
SfcI C'TryA_G
Cuts at: 0 250 460 1435 1490 2012 2931
Size: 250 210 975 55 522 919
Fragments arranged by size:
975 919 522 250 210 55
SmlI C'TyrA_G
Cuts at: 0 370 2860 2931
Size: 370 2490 71
Fragments arranged by size:
2490 370 71
SphI G_CATG'C
Cuts at: 0 2760 2931
Size: 2760 171
SspI AAT'ATT
Cuts at: 0 204 2931
Size: 204 2727
StuI AGG'CCT
Cuts at: 0 1433 2018 2931
Size: 1433 585 913
Fragments arranged by size:
1433 913 585
StyI C'CwwG_G
Cuts at: 0 435 697 801 1024 1155 1213 1298 1687
Size: 435 262 104 223 131 58 85 389
Cuts at: 1687 2373 2402 2931
Size: 686 29 529
Fragments arranged by size:
686 529 435 389 262 223 131 104
85 58 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1115 2350 2415 2534 2931
Size: 1115 1235 65 119 397
Fragments arranged by size:
1235 1115 397 119 65
TatI w'GTAC_w
Cuts at: 0 706 720 1454 2222 2931
Size: 706 14 734 768 709
Fragments arranged by size:
768 734 709 706 14
Tth111I GACn'n_nGTC
Cuts at: 0 1708 2931
Size: 1708 1223
XbaI T'CTAG_A
Cuts at: 0 450 2931
Size: 450 2481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2524 2593 2931
Size: 2524 69 338
Fragments arranged by size:
2524 338 69
XhoI C'TCGA_G
Cuts at: 0 2860 2931
Size: 2860 71
XmnI GAAnn'nnTTC
Cuts at: 0 746 1208 1612 2801 2931
Size: 746 462 404 1189 130
Fragments arranged by size:
1189 746 462 404 130
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlfI ApaI ApaLI
ApoI AseI AvaI AvrII BanI BanII BbsI BfrBI
Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrGI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI
PciI PflMI PfoI PmlI PpuMI PspOMI PspXI PsrI
PstI PvuII SacI SalI SexAI SfcI SmlI SphI
SspI StuI StyI TaqII TatI Tth111I XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AloI
AlwNI AscI AsiSI BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI BmgBI BmtI BplI
BpuEI BsaBI BsaXI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BssHII BstBI BstZ17I BtgZI ClaI
CspCI DrdI EagI EcoRV FseI HaeII HpaI KasI
KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PmeI PpiI PshAI PsiI PvuI
RsrII SacII SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TstI
XmaI ZraI