(Circular) (Six-base) MAPSORT of: pFN21ASDA0844.seq Check: 7081 from: 1 to: 5950 pFN21ASDA0844 5950 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3293 1937 Size: 1356 4594 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4013 5397 5665 1632 Size: 2381 1384 268 1917 Fragments arranged by size: 2381 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 2500 4849 5826 1203 Size: 1297 2349 977 1327 Fragments arranged by size: 2349 1327 1297 977 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5950 AlwNI CAG_nnn'CTG Cuts at: 3693 5265 3693 Size: 1572 4378 ApaI G_GGCC'C Cuts at: 2158 2158 Size: 5950 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 2513 3259 3485 3637 5746 1493 Size: 1020 746 226 152 2109 1697 Fragments arranged by size: 2109 1697 1020 746 226 152 AseI AT'TA_AT Cuts at: 160 1032 4504 160 Size: 872 3472 1606 Fragments arranged by size: 3472 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 2107 3275 1870 Size: 73 164 1168 4545 Fragments arranged by size: 4545 1168 164 73 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2994 3271 4726 618 Size: 325 217 627 1207 277 1455 1842 Fragments arranged by size: 1842 1455 1207 627 325 277 217 BanII G_rGCy'C Cuts at: 1391 2158 3168 3269 1391 Size: 767 1010 101 4072 Fragments arranged by size: 4072 1010 767 101 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4979 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4141 4175 1903 Size: 34 2204 34 3678 Fragments arranged by size: 3678 2204 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3186 4561 136 Size: 107 122 71 631 605 1514 1375 1525 Fragments arranged by size: 1525 1514 1375 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 3367 3367 Size: 5950 Bme1580I G_kGCm'C Cuts at: 1790 2158 2943 2997 4011 5167 1790 Size: 368 785 54 1014 1156 2573 Fragments arranged by size: 2573 1156 1014 785 368 54 BmgBI CAC'GTC Cuts at: 1318 2692 1318 Size: 1374 4576 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3681 4639 442 Size: 416 53 83 399 24 2264 958 1753 Fragments arranged by size: 2264 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2819 4610 1239 Size: 944 636 1791 2579 Fragments arranged by size: 2579 1791 944 636 Bpu10I CC'TnA_GC Cuts at: 2984 2984 Size: 5950 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2170 2909 3011 3115 4075 4940 5238 2170 Size: 739 102 104 960 865 298 2882 Fragments arranged by size: 2882 960 865 739 298 104 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2112 3129 4613 882 Size: 617 613 1017 1484 2219 Fragments arranged by size: 2219 1484 1017 617 613 BsaAI yAC'GTr Cuts at: 493 1204 2944 3247 3741 493 Size: 711 1740 303 494 2702 Fragments arranged by size: 2702 1740 711 494 303 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3757 4139 4774 275 Size: 53 83 186 936 2224 382 635 1451 Fragments arranged by size: 2224 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4383 4781 5055 5202 5613 4383 Size: 398 274 147 411 4720 Fragments arranged by size: 4720 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5725 Fragments arranged by size: 5725 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2756 2759 2950 1186 Size: 1570 3 191 4186 Fragments arranged by size: 4186 1570 191 3 BseYI C'CCAG_C Cuts at: 5153 5649 5153 Size: 496 5454 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2420 2648 1163 Size: 573 684 228 4465 Fragments arranged by size: 4465 684 573 228 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2453 3400 4161 5189 1540 Size: 275 186 452 947 761 1028 2301 Fragments arranged by size: 2301 1028 947 761 452 275 186 BsiHKAI G_wGCw'C Cuts at: 3168 4011 4096 5167 3168 Size: 843 85 1071 3951 Fragments arranged by size: 3951 1071 843 85 BsmI GAATG_Cn' Cuts at: 3001 3461 3554 3001 Size: 460 93 5397 Fragments arranged by size: 5397 460 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2653 2653 Size: 5950 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2158 2943 2997 3168 3269 4011 4096 Size: 399 368 785 54 171 101 742 85 Cuts at: 4096 5167 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 785 742 399 368 171 101 85 54 BspEI T'CCGG_A Cuts at: 5613 5613 Size: 5950 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3305 844 Size: 2461 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2668 3837 1394 Size: 429 845 1169 3507 Fragments arranged by size: 3507 1169 845 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4445 4619 65 Size: 1183 3197 174 1396 Fragments arranged by size: 3197 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4594 4781 1700 Size: 110 2784 187 2869 Fragments arranged by size: 2869 2784 187 110 BsrGI T'GTAC_A Cuts at: 96 2325 96 Size: 2229 3721 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 4010 5022 4010 Size: 1012 4938 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5950 BstBI TT'CG_AA Cuts at: 2007 4754 2007 Size: 2747 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5950 BstXI CCAn_nnnn'nTGG Cuts at: 2581 2581 Size: 5950 BstYI r'GATC_y Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945 Size: 1308 367 385 17 1452 86 12 346 Cuts at: 5945 1972 Size: 1977 Fragments arranged by size: 1977 1452 1308 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2415 2697 513 Size: 552 1350 282 3766 Fragments arranged by size: 3766 1350 552 282 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5211 Fragments arranged by size: 5211 683 56 BtsI GCAGTG_nn' Cuts at: 2290 2409 3466 4260 4280 2290 Size: 119 1057 794 20 3960 Fragments arranged by size: 3960 1057 794 119 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5915 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2694 3178 3397 Size: 54 1425 50 275 18 864 484 219 Cuts at: 3397 4286 5915 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 864 484 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2549 3880 1353 Size: 1196 1331 3423 Fragments arranged by size: 3423 1331 1196 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2891 4537 4911 5057 387 Size: 1064 138 1302 1646 374 146 1280 Fragments arranged by size: 1646 1302 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3166 3166 Size: 5950 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2819 4013 4610 5397 5665 1239 Size: 393 551 636 1194 597 787 268 1524 Fragments arranged by size: 1524 1194 787 636 597 551 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2965 3065 2965 Size: 100 5850 EcoO109I rG'GnC_Cy Cuts at: 2154 2456 2154 Size: 302 5648 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 EcoRV GAT'ATC Cuts at: 2054 2054 Size: 5950 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 5097 1678 Size: 3419 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2143 2175 2945 2977 3014 Size: 32 135 32 571 32 770 32 37 Cuts at: 3014 3046 4591 4623 4665 4697 1373 Size: 32 1545 32 42 32 2626 Fragments arranged by size: 2626 1545 770 571 135 42 37 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2320 3294 3540 669 Size: 988 281 382 974 246 3079 Fragments arranged by size: 3079 988 974 382 281 246 HindIII A'AGCT_T Cuts at: 748 2137 2484 748 Size: 1389 347 4214 Fragments arranged by size: 4214 1389 347 HpaI GTT'AAC Cuts at: 2320 3540 2320 Size: 1220 4730 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2586 5064 5248 1305 Size: 1281 2478 184 2007 Fragments arranged by size: 2478 2007 1281 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4128 Fragments arranged by size: 4128 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 3908 4267 4426 518 Size: 648 1248 1494 359 159 2042 Fragments arranged by size: 2042 1494 1248 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2632 2804 2863 2937 3366 4043 5191 5436 Size: 1026 172 59 74 429 677 1148 245 Cuts at: 5436 1606 Size: 2120 Fragments arranged by size: 2120 1148 1026 677 429 245 172 74 59 NcoI C'CATG_G Cuts at: 513 1065 2415 513 Size: 552 1350 4048 Fragments arranged by size: 4048 1350 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 2504 3094 3308 4853 1132 Size: 97 1275 590 214 1545 2229 Fragments arranged by size: 2229 1545 1275 590 214 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 2500 4849 2500 Size: 2349 3601 PflMI CCAn_nnn'nTGG Cuts at: 1108 2791 1108 Size: 1683 4267 PfoI T'CCnGG_A Cuts at: 2458 2790 2835 2458 Size: 332 45 5573 Fragments arranged by size: 5573 332 45 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PmlI CAC'GTG Cuts at: 2944 2944 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2456 2456 Size: 5950 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PspOMI G'GGCC_C Cuts at: 2154 2154 Size: 5950 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3227 3259 3227 Size: 32 5918 PstI C_TGCA'G Cuts at: 830 3146 3302 830 Size: 2316 156 3478 Fragments arranged by size: 3478 2316 156 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 PvuII CAG'CTG Cuts at: 2632 2804 2632 Size: 172 5778 SacI G_AGCT'C Cuts at: 3168 3168 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 2894 4198 5910 2894 Size: 1304 1712 2934 Fragments arranged by size: 2934 1712 1304 SfcI C'TryA_G Cuts at: 826 1045 3142 3298 4433 5114 5305 826 Size: 219 2097 156 1135 681 191 1471 Fragments arranged by size: 2097 1471 1135 681 219 191 156 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2149 2888 3026 3130 4054 4955 Size: 197 926 206 739 138 104 924 901 Cuts at: 4955 5217 820 Size: 262 1553 Fragments arranged by size: 1553 926 924 901 739 262 206 197 138 104 SnaBI TAC'GTA Cuts at: 493 3247 3741 493 Size: 2754 494 2702 Fragments arranged by size: 2754 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3094 3308 1132 Size: 1962 214 3774 Fragments arranged by size: 3774 1962 214 SspI AAT'ATT Cuts at: 5 52 3874 5 Size: 47 3822 2081 Fragments arranged by size: 3822 2081 47 StuI AGG'CCT Cuts at: 1517 2386 2912 1517 Size: 869 526 4555 Fragments arranged by size: 4555 869 526 StyI C'CwwG_G Cuts at: 513 1065 2349 2415 3072 3102 3389 513 Size: 552 1284 66 657 30 287 3074 Fragments arranged by size: 3074 1284 657 552 287 66 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3057 3977 3994 4147 4332 4777 325 Size: 1255 1477 920 17 153 185 445 1498 Fragments arranged by size: 1498 1477 1255 920 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 2713 2892 Size: 274 80 33 51 1443 348 388 179 Cuts at: 2892 4196 5908 96 Size: 1304 1712 138 Fragments arranged by size: 1712 1443 1304 388 348 274 179 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5910 5942 5910 Size: 32 5918 Tth111I GACn'n_nGTC Cuts at: 1316 2240 2449 1316 Size: 924 209 4817 Fragments arranged by size: 4817 924 209 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2912 2912 Size: 5950 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 4079 4079 Size: 5950 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI