Restriction map for
pFN21ASDA0843
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MslI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccII 106 CGCG CG^CG
AflIII 1004 ACATGT A^C[GA][CT]GT
Alw44I 109 GTGCAC G^TGCAC
ApaI 1204 GGGCCC GGGCC^C
ApaLI 109 GTGCAC G^TGCAC
Asp700I 336 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 410 TGGCCA TGG^CCA
BamHI 853 GGATCC G^GATCC
BbsI 1387 GAAGAC GAAGAC(2/6)
BfaI 8 CTAG C^TAG
BglI 1148 GCCGGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1856 AGATCT A^GATCT
BpuAI 1387 GAAGAC GAAGAC(2/6)
BsaWI 1322 ACCGGA [AT]^CCGG[AT]
BsmBI 1104 GAGACG CGTCTC(1/5)
BsmI 11 GCATTC GAATGC(1/-1)
BspLU11I 1004 ACATGT A^CATGT
BstAPI 323 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 106 CGCG CG^CG
BstXI 1965 CCAAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
HaeII 1937 AGCGCC [GA]GCGC^[CT]
HgaI 545 GCGTC GACGC(5/10)
HincII 1831 GTCAAC GT[CT]^[GA]AC
HindII 1831 GTCAAC GT[CT]^[GA]AC
MaeI 8 CTAG C^TAG
MluNI 410 TGGCCA TGG^CCA
MscI 410 TGGCCA TGG^CCA
MvnI 106 CGCG CG^CG
PflMI 402 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 540 CTGCAG CTGCA^G
ScaI 1457 AGTACT AGT^ACT
TaqI 1603 TCGA T^CGA
TthHB8I 1603 TCGA T^CGA
Van91I 402 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 336 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 1359 GTCTAC GT^[AC][GT]AC
1918 GTAGAC
BanI 235 GGCACC G^G[CT][GA]CC
770 GGCACC
BcgI 600 CGACTACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
877 CGAGTCATCTGC
BsiEI 1600 CGGTCG CG[GA][CT]^CG
1705 CGGTCG
BspMI 85 ACCTGC ACCTGC(4/8)
538 ACCTGC
BsrBI 1176 CCGCTC CCGCTC(-3/-3)
1197 CCGCTC
BstEII 360 GGTGACC G^GT[ACGT]ACC
866 GGTCACC
BstPI 360 GGTGACC G^GT[ACGT]ACC
866 GGTCACC
DdeI 1454 CTGAG C^T[ACGT]AG
1988 CTGAG
EcoNI 695 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1944 CCTGTCCCAGG
EcoO65I 360 GGTGACC G^GT[ACGT]ACC
866 GGTCACC
FauI 239 CCCGC CCCGC(4/6)
1175 CCCGC
MaeII 394 ACGT A^CGT
515 ACGT
NaeI 1148 GCCGGC GCC^GGC
1568 GCCGGC
NcoI 407 CCATGG C^CATGG
1470 CCATGG
NgoMIV 1148 GCCGGC G^CCGGC
1568 GCCGGC
SfaNI 134 GATGC GCATC(5/9)
1017 GATGC
SmaI 794 CCCGGG CCC^GGG
1119 CCCGGG
SphI 1541 GCATGC GCATG^C
1841 GCATGC
Tsp509I 913 AATT ^AATT
1563 AATT
XmaCI 794 CCCGGG C^CCGGG
1119 CCCGGG
XmaI 794 CCCGGG C^CCGGG
1119 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfcI
StyI TfiI Tru9I TseI Tsp45I
TspRI XcmI XhoII