(Circular) (Six-base) MAPSORT of: pFN21ASDA0843.seq Check: 7959 from: 1 to: 6778 pFN21ASDA0843 6778 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2117 2117 Size: 6778 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4588 278 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3383 3942 4121 1937 Size: 1446 559 179 4594 Fragments arranged by size: 4594 1446 559 179 Acc65I G'GTAC_C Cuts at: 4099 4099 Size: 6778 AclI AA'CG_TT Cuts at: 1563 4905 5278 1563 Size: 3342 373 3063 Fragments arranged by size: 3342 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2374 3623 4070 4841 6225 6493 1632 Size: 742 1249 447 771 1384 268 1917 Fragments arranged by size: 1917 1384 1249 771 742 447 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6581 AflIII A'CryG_T Cuts at: 1203 3027 5677 6654 1203 Size: 1824 2650 977 1327 Fragments arranged by size: 2650 1824 1327 977 AgeI A'CCGG_T Cuts at: 5609 5609 Size: 6778 AhdI GACnn_n'nnGTC Cuts at: 5507 5507 Size: 6778 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6778 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6778 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3067 3099 3067 Size: 32 6746 AlwNI CAG_nnn'CTG Cuts at: 4521 6093 4521 Size: 1572 5206 ApaI G_GGCC'C Cuts at: 3231 3231 Size: 6778 ApaLI G'TGCA_C Cuts at: 2132 4835 5991 2132 Size: 2703 1156 2919 Fragments arranged by size: 2919 2703 1156 ApoI r'AATT_y Cuts at: 1493 4087 4313 4465 6574 1493 Size: 2594 226 152 2109 1697 Fragments arranged by size: 2594 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5332 160 Size: 872 4300 1606 Fragments arranged by size: 4300 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 AvaI C'yCGr_G Cuts at: 1870 1943 2817 3142 3230 3513 4103 1870 Size: 73 874 325 88 283 590 4545 Fragments arranged by size: 4545 874 590 325 283 88 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2659 2692 2659 Size: 33 6745 BamHI G'GATC_C Cuts at: 2876 4108 4475 2876 Size: 1232 367 5179 Fragments arranged by size: 5179 1232 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2258 2793 4099 5554 618 Size: 325 217 627 471 535 1306 1455 1842 Fragments arranged by size: 1842 1455 1306 627 535 471 325 217 BanII G_rGCy'C Cuts at: 1391 3231 3248 3632 3665 4097 1391 Size: 1840 17 384 33 432 4072 Fragments arranged by size: 4072 1840 432 384 33 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3417 928 Size: 971 1518 4289 Fragments arranged by size: 4289 1518 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2612 2646 2889 2923 4969 5003 1903 Size: 34 675 34 243 34 2046 34 3678 Fragments arranged by size: 3678 2046 675 243 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4670 5880 4670 Size: 1210 5568 BclI T'GATC_A Cuts at: 1548 1548 Size: 6778 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3177 5389 136 Size: 107 122 71 631 605 1505 2212 1525 Fragments arranged by size: 2212 1525 1505 631 605 122 107 71 BglII A'GATC_T Cuts at: 3879 6773 3879 Size: 2894 3884 BlpI GC'TnA_GC Cuts at: 4195 4195 Size: 6778 Bme1580I G_kGCm'C Cuts at: 1790 2136 3231 3513 4839 5995 1790 Size: 346 1095 282 1326 1156 2573 Fragments arranged by size: 2573 1326 1156 1095 346 282 BmgBI CAC'GTC Cuts at: 1318 2539 1318 Size: 1221 5557 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2188 2419 2629 Size: 416 53 83 399 24 771 231 210 Cuts at: 2629 3868 4509 5467 442 Size: 1239 641 958 1753 Fragments arranged by size: 1753 1239 958 771 641 416 399 231 210 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6778 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3065 3121 3348 3555 5438 1239 Size: 1826 56 227 207 1883 2579 Fragments arranged by size: 2579 1883 1826 227 207 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4903 5768 6066 4903 Size: 865 298 5615 Fragments arranged by size: 5615 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5441 882 Size: 617 3942 2219 Fragments arranged by size: 3942 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4075 4569 493 Size: 711 2871 494 2702 Fragments arranged by size: 2871 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6772 6772 Size: 6778 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4585 4967 5602 275 Size: 53 83 186 936 3052 382 635 1451 Fragments arranged by size: 3052 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3345 5211 5609 5883 6030 6441 3345 Size: 1866 398 274 147 411 3682 Fragments arranged by size: 3682 1866 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2367 2397 3067 3097 3140 Size: 30 165 30 964 30 670 30 43 Cuts at: 3140 3170 3464 3494 1178 Size: 30 294 30 4462 Fragments arranged by size: 4462 964 670 294 165 43 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2504 3552 3557 1186 Size: 1318 1048 5 4407 Fragments arranged by size: 4407 1318 1048 5 BseYI C'CCAG_C Cuts at: 3070 3322 3815 5981 6477 3070 Size: 252 493 2166 496 3371 Fragments arranged by size: 3371 2166 496 493 252 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2215 2365 2374 3671 3788 1163 Size: 573 479 150 9 1297 117 4153 Fragments arranged by size: 4153 1297 573 479 150 117 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3626 3731 4228 4989 6017 1540 Size: 275 186 1625 105 497 761 1028 2301 Fragments arranged by size: 2301 1625 1028 761 497 275 186 105 BsiHKAI G_wGCw'C Cuts at: 2136 2423 3248 3632 3665 4839 4924 5995 2136 Size: 287 825 384 33 1174 85 1071 2919 Fragments arranged by size: 2919 1174 1071 825 384 287 85 33 BsmI GAATG_Cn' Cuts at: 2034 4289 4382 2034 Size: 2255 93 4430 Fragments arranged by size: 4430 2255 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3121 3121 Size: 6778 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2423 3231 3248 3513 3632 3665 Size: 399 346 287 808 17 265 119 33 Cuts at: 3665 4097 4839 4924 5995 1391 Size: 432 742 85 1071 2174 Fragments arranged by size: 2174 1071 808 742 432 399 346 287 265 119 85 33 17 BspEI T'CCGG_A Cuts at: 6441 6441 Size: 6778 BspHI T'CATG_A Cuts at: 4667 6397 4667 Size: 1730 5048 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2117 2570 4133 844 Size: 1273 453 1563 3489 Fragments arranged by size: 3489 1563 1273 453 BsrBI CCG'CTC Cuts at: 1394 1823 3201 3222 4665 1394 Size: 429 1378 21 1443 3507 Fragments arranged by size: 3507 1443 1378 429 21 BsrDI GCAATG_nn' Cuts at: 65 1248 5273 5447 65 Size: 1183 4025 174 1396 Fragments arranged by size: 4025 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3171 3591 4020 5422 5609 1700 Size: 110 1361 420 429 1402 187 2869 Fragments arranged by size: 2869 1402 1361 429 420 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6778 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6778 BssSI C'ACGA_G Cuts at: 4838 5850 4838 Size: 1012 5766 BstAPI GCAn_nnn'nTGC Cuts at: 794 2352 794 Size: 1558 5220 BstBI TT'CG_AA Cuts at: 2007 5582 2007 Size: 3575 3203 BstEII G'GTnAC_C Cuts at: 1184 2383 2889 1184 Size: 1199 506 5073 Fragments arranged by size: 5073 1199 506 BstXI CCAn_nnnn'nTGG Cuts at: 3995 3995 Size: 6778 BstYI r'GATC_y Cuts at: 1972 2876 2928 3640 3879 4108 4475 4860 4877 Size: 904 52 712 239 229 367 385 17 Cuts at: 4877 6329 6415 6427 6773 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 904 712 385 367 346 239 229 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2063 2430 3493 513 Size: 552 998 367 1063 3798 Fragments arranged by size: 3798 1063 998 552 367 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6039 Fragments arranged by size: 6039 683 56 BtsI GCAGTG_nn' Cuts at: 2537 4294 5088 5108 2537 Size: 1757 794 20 4207 Fragments arranged by size: 4207 1757 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6778 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2440 2475 529 Size: 35 1876 35 4832 Fragments arranged by size: 4832 1876 35 35 DraI TTT'AAA Cuts at: 4082 4082 Size: 6778 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5785 809 Size: 507 4469 1802 Fragments arranged by size: 4469 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2433 2503 3238 Size: 54 1425 50 275 18 603 70 735 Cuts at: 3238 3589 4225 5114 6743 8 Size: 351 636 889 1629 43 Fragments arranged by size: 1629 1425 889 735 636 603 351 275 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4225 1537 Size: 275 2413 4090 Fragments arranged by size: 4090 2413 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3833 3972 4031 4708 1353 Size: 2480 139 59 677 3423 Fragments arranged by size: 3423 2480 677 139 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5365 5739 5885 387 Size: 1064 138 3776 374 146 1280 Fragments arranged by size: 3776 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3246 3630 3663 3246 Size: 384 33 6361 Fragments arranged by size: 6361 384 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2374 3065 3121 3348 3555 3623 4070 Size: 393 742 691 56 227 207 68 447 Cuts at: 4070 4841 5438 6225 6493 1239 Size: 771 597 787 268 1524 Fragments arranged by size: 1524 787 771 742 691 597 447 393 268 227 207 68 56 EcoNI CCTnn'n_nnAGG Cuts at: 2722 3971 2722 Size: 1249 5529 EcoO109I rG'GnC_Cy Cuts at: 3276 3498 3948 3276 Size: 222 450 6106 Fragments arranged by size: 6106 450 222 EcoRI G'AATT_C Cuts at: 4087 4465 4087 Size: 378 6400 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2404 2404 Size: 6778 FspI TGC'GCA Cuts at: 5284 5284 Size: 6778 HaeII r_GCGC'y Cuts at: 1678 3964 5925 1678 Size: 2286 1961 2531 Fragments arranged by size: 2531 2286 1961 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3260 3292 5419 5451 5493 Size: 32 135 32 1688 32 2127 32 42 Cuts at: 5493 5525 1373 Size: 32 2626 Fragments arranged by size: 2626 2127 1688 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3856 4122 4368 669 Size: 988 281 1918 266 246 3079 Fragments arranged by size: 3079 1918 988 281 266 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6778 HpaI GTT'AAC Cuts at: 4368 4368 Size: 6778 KpnI G_GTAC'C Cuts at: 4103 4103 Size: 6778 MfeI C'AATT_G Cuts at: 4377 4377 Size: 6778 MluI A'CGCG_T Cuts at: 6654 6654 Size: 6778 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2646 3057 3311 5892 6076 1305 Size: 1341 411 254 2581 184 2007 Fragments arranged by size: 2581 2007 1341 411 254 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 10 Size: 54 1425 343 603 4353 Fragments arranged by size: 4353 1425 603 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4736 5095 5254 518 Size: 648 3570 359 159 2042 Fragments arranged by size: 3570 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2097 2265 2274 2394 2544 3326 3695 4194 Size: 491 168 9 120 150 782 369 499 Cuts at: 4194 4871 6019 6264 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 782 677 499 491 369 245 168 150 120 9 NaeI GCC'GGC Cuts at: 3173 3593 3173 Size: 420 6358 NcoI C'CATG_G Cuts at: 513 1065 2430 3493 513 Size: 552 1365 1063 3798 Fragments arranged by size: 3798 1365 1063 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6778 NgoMIV G'CCGG_C Cuts at: 3171 3591 3171 Size: 420 6358 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6778 NotI GC'GGCC_GC Cuts at: 4225 4225 Size: 6778 NspI r_CATG'y Cuts at: 1132 1229 3031 3184 3568 3868 4136 5681 1132 Size: 97 1802 153 384 300 268 1545 2229 Fragments arranged by size: 2229 1802 1545 384 300 268 153 97 PasI CC'CwG_GG Cuts at: 1348 2311 1348 Size: 963 5815 PciI A'CATG_T Cuts at: 3027 5677 3027 Size: 2650 4128 PflMI CCAn_nnn'nTGG Cuts at: 1108 2431 1108 Size: 1323 5455 PmeI GTTT'AAAC Cuts at: 4082 4082 Size: 6778 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3067 3099 4847 4879 6360 6392 3067 Size: 32 1748 32 1481 32 3453 Fragments arranged by size: 3453 1748 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3276 3498 3948 3276 Size: 222 450 6106 Fragments arranged by size: 6106 450 222 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6778 PsiI TTA'TAA Cuts at: 4348 4348 Size: 6778 PspOMI G'GGCC_C Cuts at: 3227 3227 Size: 6778 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2207 2239 2207 Size: 32 6746 PstI C_TGCA'G Cuts at: 830 2567 4130 830 Size: 1737 1563 3478 Fragments arranged by size: 3478 1737 1563 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6778 PvuII CAG'CTG Cuts at: 2274 2544 3326 3695 2274 Size: 270 782 369 5357 Fragments arranged by size: 5357 782 369 270 SacI G_AGCT'C Cuts at: 3248 3632 3665 3248 Size: 384 33 6361 Fragments arranged by size: 6361 384 33 SalI G'TCGA_C Cuts at: 1936 4120 1936 Size: 2184 4594 SanDI GG'GwC_CC Cuts at: 3498 3498 Size: 6778 SbfI CC_TGCA'GG Cuts at: 4130 4130 Size: 6778 ScaI AGT'ACT Cuts at: 3482 5026 6738 3482 Size: 1544 1712 3522 Fragments arranged by size: 3522 1712 1544 SfcI C'TryA_G Cuts at: 826 1045 2563 3166 3384 4126 5261 5942 6133 Size: 219 1518 603 218 742 1135 681 191 Cuts at: 6133 826 Size: 1471 Fragments arranged by size: 1518 1471 1135 742 681 603 219 218 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6778 SmaI CCC'GGG Cuts at: 1872 2819 3144 4105 1872 Size: 947 325 961 4545 Fragments arranged by size: 4545 961 947 325 SmlI C'TyrA_G Cuts at: 820 1017 1943 4882 5783 6045 820 Size: 197 926 2939 901 262 1553 Fragments arranged by size: 2939 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4075 4569 493 Size: 3582 494 2702 Fragments arranged by size: 3582 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6778 SphI G_CATG'C Cuts at: 1132 3568 3868 4136 1132 Size: 2436 300 268 3774 Fragments arranged by size: 3774 2436 300 268 SrfI GCCC'GGGC Cuts at: 2819 2819 Size: 6778 SspI AAT'ATT Cuts at: 5 52 4702 5 Size: 47 4650 2081 Fragments arranged by size: 4650 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6778 StyI C'CwwG_G Cuts at: 513 1065 2430 2961 3493 3933 4217 513 Size: 552 1365 531 532 440 284 3074 Fragments arranged by size: 3074 1365 552 532 531 440 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2249 2679 2727 2908 3717 3829 4805 Size: 1255 669 430 48 181 809 112 976 Cuts at: 4805 4822 4975 5160 5605 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 976 809 669 445 430 185 181 153 112 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2223 3432 3480 Size: 274 80 33 51 1443 246 1209 48 Cuts at: 3480 3873 5024 6736 96 Size: 393 1151 1712 138 Fragments arranged by size: 1712 1443 1209 1151 393 274 246 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2020 2052 2367 2399 2516 2548 6738 6770 2020 Size: 32 315 32 117 32 4190 32 2028 Fragments arranged by size: 4190 2028 315 117 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6778 XbaI T'CTAG_A Cuts at: 4114 4114 Size: 6778 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2252 2636 3584 2252 Size: 384 948 5446 Fragments arranged by size: 5446 948 384 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6778 XmaI C'CCGG_G Cuts at: 1870 2817 3142 4103 1870 Size: 947 325 961 4545 Fragments arranged by size: 4545 961 947 325 XmnI GAAnn'nnTTC Cuts at: 2363 4907 2363 Size: 2544 4234 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4586 276 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI RsrII SacII SapI SexAI SfiI SfoI SwaI