Restriction map for
pFN21ASDA0843
(Linear) (Six-base) MAPSORT of: KIAA0843.seq Check: 9720 from: 1 to: 2049
KIAA0843.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 94 2049
Size: 94 1955
AccI GT'mk_AC
Cuts at: 0 1360 1919 2049
Size: 1360 559 130
Fragments arranged by size:
1360 559 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1600 2047 2049
Size: 351 1249 447 2
Fragments arranged by size:
1249 447 351 2
AflIII A'CryG_T
Cuts at: 0 1004 2049
Size: 1004 1045
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1044 1076 2049
Size: 1044 32 973
Fragments arranged by size:
1044 973 32
ApaI G_GGCC'C
Cuts at: 0 1208 2049
Size: 1208 841
ApaLI G'TGCA_C
Cuts at: 0 109 2049
Size: 109 1940
AvaI C'yCGr_G
Cuts at: 0 794 1119 1207 1490 2049
Size: 794 325 88 283 559
Fragments arranged by size:
794 559 325 283 88
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 636 669 2049
Size: 636 33 1380
Fragments arranged by size:
1380 636 33
BamHI G'GATC_C
Cuts at: 0 853 2049
Size: 853 1196
BanI G'GyrC_C
Cuts at: 0 235 770 2049
Size: 235 535 1279
Fragments arranged by size:
1279 535 235
BanII G_rGCy'C
Cuts at: 0 1208 1225 1609 1642 2049
Size: 1208 17 384 33 407
Fragments arranged by size:
1208 407 384 33 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 1394 2049
Size: 1394 655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 589 623 866 900 2049
Size: 589 34 243 34 1149
Fragments arranged by size:
1149 589 243 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1154 2049
Size: 1154 895
BglII A'GATC_T
Cuts at: 0 1856 2049
Size: 1856 193
Bme1580I G_kGCm'C
Cuts at: 0 113 1208 1490 2049
Size: 113 1095 282 559
Fragments arranged by size:
1095 559 282 113
BmgBI CAC'GTC
Cuts at: 0 516 2049
Size: 516 1533
BmrI ACTGGGnnnn_n'
Cuts at: 0 165 396 606 1845 2049
Size: 165 231 210 1239 204
Fragments arranged by size:
1239 231 210 204 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1042 1098 1325 1532 2049
Size: 1042 56 227 207 517
Fragments arranged by size:
1042 517 227 207 56
BsaWI w'CCGG_w
Cuts at: 0 1322 2049
Size: 1322 727
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 344 374 1044 1074 1117 1147 1441 1471
Size: 344 30 670 30 43 30 294 30
Cuts at: 1471 2049
Size: 578
Fragments arranged by size:
670 578 344 294 43 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 481 1529 1534 2049
Size: 481 1048 5 515
Fragments arranged by size:
1048 515 481 5
BseYI C'CCAG_C
Cuts at: 0 1047 1299 1792 2049
Size: 1047 252 493 257
Fragments arranged by size:
1047 493 257 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 342 351 1648 1765 2049
Size: 192 150 9 1297 117 284
Fragments arranged by size:
1297 284 192 150 117 9
BsiEI CG_ry'CG
Cuts at: 0 1603 1708 2049
Size: 1603 105 341
Fragments arranged by size:
1603 341 105
BsiHKAI G_wGCw'C
Cuts at: 0 113 400 1225 1609 1642 2049
Size: 113 287 825 384 33 407
Fragments arranged by size:
825 407 384 287 113 33
BsmI GAATG_Cn'
Cuts at: 0 11 2049
Size: 11 2038
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1098 2049
Size: 1098 951
Bsp1286I G_dGCh'C
Cuts at: 0 113 400 1208 1225 1490 1609 1642 2049
Size: 113 287 808 17 265 119 33 407
Fragments arranged by size:
808 407 287 265 119 113 33 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 547 2049
Size: 94 453 1502
Fragments arranged by size:
1502 453 94
BsrBI CCG'CTC
Cuts at: 0 1178 1199 2049
Size: 1178 21 850
Fragments arranged by size:
1178 850 21
BsrFI r'CCGG_y
Cuts at: 0 1148 1568 1997 2049
Size: 1148 420 429 52
Fragments arranged by size:
1148 429 420 52
BstAPI GCAn_nnn'nTGC
Cuts at: 0 329 2049
Size: 329 1720
BstEII G'GTnAC_C
Cuts at: 0 360 866 2049
Size: 360 506 1183
Fragments arranged by size:
1183 506 360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1972 2049
Size: 1972 77
BstYI r'GATC_y
Cuts at: 0 853 905 1617 1856 2049
Size: 853 52 712 239 193
Fragments arranged by size:
853 712 239 193 52
BtgI C'CryG_G
Cuts at: 0 40 407 1470 2049
Size: 40 367 1063 579
Fragments arranged by size:
1063 579 367 40
BtsI GCAGTG_nn'
Cuts at: 0 514 2049
Size: 514 1535
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 417 452 2049
Size: 417 35 1597
Fragments arranged by size:
1597 417 35
EaeI y'GGCC_r
Cuts at: 0 410 480 1215 1566 2049
Size: 410 70 735 351 483
Fragments arranged by size:
735 483 410 351 70
EarI CTCTTCn'nnn_
Cuts at: 0 1810 1949 2008 2049
Size: 1810 139 59 41
Fragments arranged by size:
1810 139 59 41
EcoICRI GAG'CTC
Cuts at: 0 1223 1607 1640 2049
Size: 1223 384 33 409
Fragments arranged by size:
1223 409 384 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1042 1098 1325 1532 1600 2047 2049
Size: 351 691 56 227 207 68 447 2
Fragments arranged by size:
691 447 351 227 207 68 56 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 699 1948 2049
Size: 699 1249 101
Fragments arranged by size:
1249 699 101
EcoO109I rG'GnC_Cy
Cuts at: 0 1253 1475 1925 2049
Size: 1253 222 450 124
Fragments arranged by size:
1253 450 222 124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 381 2049
Size: 381 1668
HaeII r_GCGC'y
Cuts at: 0 1941 2049
Size: 1941 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1237 1269 2049
Size: 1237 32 780
Fragments arranged by size:
1237 780 32
HincII GTy'rAC
Cuts at: 0 1833 2049
Size: 1833 216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 1034 1288 2049
Size: 623 411 254 761
Fragments arranged by size:
761 623 411 254
MscI TGG'CCA
Cuts at: 0 412 2049
Size: 412 1637
MspA1I CmG'CkG
Cuts at: 0 74 242 251 371 521 1303 1672 2049
Size: 74 168 9 120 150 782 369 377
Fragments arranged by size:
782 377 369 168 150 120 74 9
NaeI GCC'GGC
Cuts at: 0 1150 1570 2049
Size: 1150 420 479
Fragments arranged by size:
1150 479 420
NcoI C'CATG_G
Cuts at: 0 407 1470 2049
Size: 407 1063 579
Fragments arranged by size:
1063 579 407
NgoMIV G'CCGG_C
Cuts at: 0 1148 1568 2049
Size: 1148 420 481
Fragments arranged by size:
1148 481 420
NspI r_CATG'y
Cuts at: 0 1008 1161 1545 1845 2049
Size: 1008 153 384 300 204
Fragments arranged by size:
1008 384 300 204 153
PasI CC'CwG_GG
Cuts at: 0 288 2049
Size: 288 1761
PciI A'CATG_T
Cuts at: 0 1004 2049
Size: 1004 1045
PflMI CCAn_nnn'nTGG
Cuts at: 0 408 2049
Size: 408 1641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1044 1076 2049
Size: 1044 32 973
Fragments arranged by size:
1044 973 32
PpuMI rG'GwC_Cy
Cuts at: 0 1253 1475 1925 2049
Size: 1253 222 450 124
Fragments arranged by size:
1253 450 222 124
PspOMI G'GGCC_C
Cuts at: 0 1204 2049
Size: 1204 845
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 184 216 2049
Size: 184 32 1833
Fragments arranged by size:
1833 184 32
PstI C_TGCA'G
Cuts at: 0 544 2049
Size: 544 1505
PvuII CAG'CTG
Cuts at: 0 251 521 1303 1672 2049
Size: 251 270 782 369 377
Fragments arranged by size:
782 377 369 270 251
SacI G_AGCT'C
Cuts at: 0 1225 1609 1642 2049
Size: 1225 384 33 407
Fragments arranged by size:
1225 407 384 33
SanDI GG'GwC_CC
Cuts at: 0 1475 2049
Size: 1475 574
ScaI AGT'ACT
Cuts at: 0 1459 2049
Size: 1459 590
SfcI C'TryA_G
Cuts at: 0 540 1143 1361 2049
Size: 540 603 218 688
Fragments arranged by size:
688 603 540 218
SmaI CCC'GGG
Cuts at: 0 796 1121 2049
Size: 796 325 928
Fragments arranged by size:
928 796 325
SphI G_CATG'C
Cuts at: 0 1545 1845 2049
Size: 1545 300 204
Fragments arranged by size:
1545 300 204
SrfI GCCC'GGGC
Cuts at: 0 796 2049
Size: 796 1253
StyI C'CwwG_G
Cuts at: 0 407 938 1470 1910 2049
Size: 407 531 532 440 139
Fragments arranged by size:
532 531 440 407 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 656 704 885 1694 1806 2049
Size: 226 430 48 181 809 112 243
Fragments arranged by size:
809 430 243 226 181 112 48
TatI w'GTAC_w
Cuts at: 0 200 1409 1457 1850 2049
Size: 200 1209 48 393 199
Fragments arranged by size:
1209 393 200 199 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 29 344 376 493 525 2049
Size: 29 315 32 117 32 1524
Fragments arranged by size:
1524 315 117 32 32 29
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 229 613 1561 2049
Size: 229 384 948 488
Fragments arranged by size:
948 488 384 229
XmaI C'CCGG_G
Cuts at: 0 794 1119 2049
Size: 794 325 930
Fragments arranged by size:
930 794 325
XmnI GAAnn'nnTTC
Cuts at: 0 340 2049
Size: 340 1709
Enzymes that do cut:
AarI AccI AcuI AflIII AloI ApaI ApaLI AvaI
BaeI BamHI BanI BanII BbsI BcgI BglI BglII
Bme1580I BmgBI BmrI BpmI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII MmeI MscI MspA1I NaeI NcoI NgoMIV
NspI PasI PciI PflMI PpiI PpuMI PspOMI PsrI
PstI PvuII SacI SanDI ScaI SfcI SmaI SphI
SrfI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AlwNI ApoI AscI AseI AsiSI AvrII BbeI
BbvCI BciVI BclI BfrBI BlpI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsiWI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII DrdI EagI EciI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MslI NarI NdeI NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PshAI PsiI PspXI PvuI
RsrII SacII SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SspI StuI SwaI
Tth111I XbaI XhoI ZraI