(Circular) (Six-base) MAPSORT of: pFN21ASDA0841.seq Check: 5009 from: 1 to: 6628 pFN21ASDA0841 6628 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3031 3031 Size: 6628 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4438 278 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3971 1937 Size: 2034 4594 Acc65I G'GTAC_C Cuts at: 3949 3949 Size: 6628 AclI AA'CG_TT Cuts at: 1563 4755 5128 1563 Size: 3192 373 3063 Fragments arranged by size: 3192 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2978 3755 4691 6075 6343 1632 Size: 1346 777 936 1384 268 1917 Fragments arranged by size: 1917 1384 1346 936 777 268 AfeI AGC'GCT Cuts at: 3507 3507 Size: 6628 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6431 AflIII A'CryG_T Cuts at: 1203 3016 5527 6504 1203 Size: 1813 2511 977 1327 Fragments arranged by size: 2511 1813 1327 977 AgeI A'CCGG_T Cuts at: 5459 5459 Size: 6628 AhdI GACnn_n'nnGTC Cuts at: 5357 5357 Size: 6628 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6628 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2790 3425 1184 Size: 1606 635 4387 Fragments arranged by size: 4387 1606 635 AlwNI CAG_nnn'CTG Cuts at: 2314 2470 2617 2704 2881 2995 3046 3115 3145 Size: 156 147 87 177 114 51 69 30 Cuts at: 3145 3676 3733 3766 3856 3913 4371 5943 2314 Size: 531 57 33 90 57 458 1572 2999 Fragments arranged by size: 2999 1572 531 458 177 156 147 114 90 87 69 57 57 51 33 30 ApaI G_GGCC'C Cuts at: 2101 2416 2611 3904 2101 Size: 315 195 1293 4825 Fragments arranged by size: 4825 1293 315 195 ApaLI G'TGCA_C Cuts at: 2171 4685 5841 2171 Size: 2514 1156 2958 Fragments arranged by size: 2958 2514 1156 ApoI r'AATT_y Cuts at: 1493 3937 4163 4315 6424 1493 Size: 2444 226 152 2109 1697 Fragments arranged by size: 2444 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5182 160 Size: 872 4150 1606 Fragments arranged by size: 4150 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 AvaI C'yCGr_G Cuts at: 1870 1943 2094 3274 3953 1870 Size: 73 151 1180 679 4545 Fragments arranged by size: 4545 1180 679 151 73 BamHI G'GATC_C Cuts at: 2534 3026 3177 3958 4325 2534 Size: 492 151 781 367 4837 Fragments arranged by size: 4837 781 492 367 151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2080 2487 3189 3331 3949 Size: 325 217 627 293 407 702 142 618 Cuts at: 3949 5404 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 702 627 618 407 325 293 217 142 BanII G_rGCy'C Cuts at: 1391 2101 2401 2416 2568 2611 2711 3087 3275 Size: 710 300 15 152 43 100 376 188 Cuts at: 3275 3494 3846 3904 3947 1391 Size: 219 352 58 43 4072 Fragments arranged by size: 4072 710 376 352 300 219 188 152 100 58 43 43 15 BbeI G_GCGC'C Cuts at: 2491 3193 2491 Size: 702 5926 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2440 3647 928 Size: 971 541 1207 3909 Fragments arranged by size: 3909 1207 971 541 BbvCI CC'TCA_GC Cuts at: 3149 3149 Size: 6628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2577 2611 4819 4853 1903 Size: 34 640 34 2208 34 3678 Fragments arranged by size: 3678 2208 640 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4520 5730 4520 Size: 1210 5418 BclI T'GATC_A Cuts at: 1548 1548 Size: 6628 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2991 3871 3899 Size: 107 122 71 631 605 1319 880 28 Cuts at: 3899 5239 136 Size: 1340 1525 Fragments arranged by size: 1525 1340 1319 880 631 605 122 107 71 28 BglII A'GATC_T Cuts at: 6623 6623 Size: 6628 BlpI GC'TnA_GC Cuts at: 2705 3509 4045 2705 Size: 804 536 5288 Fragments arranged by size: 5288 804 536 Bme1580I G_kGCm'C Cuts at: 1790 2085 2101 2175 2416 2611 3404 3904 4689 Size: 295 16 74 241 195 793 500 785 Cuts at: 4689 5845 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 793 785 500 295 241 195 74 16 BmgBI CAC'GTC Cuts at: 1318 2369 2544 2749 1318 Size: 1051 175 205 5197 Fragments arranged by size: 5197 1051 205 175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2060 2605 3062 Size: 416 53 83 399 24 643 545 457 Cuts at: 3062 3862 4359 5317 442 Size: 800 497 958 1753 Fragments arranged by size: 1753 958 800 643 545 497 457 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2033 3290 1056 Size: 977 1257 4394 Fragments arranged by size: 4394 1257 977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2420 2420 Size: 6628 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2582 2854 2972 3383 3478 3516 3764 Size: 1097 246 272 118 411 95 38 248 Cuts at: 3764 3788 5288 1239 Size: 24 1500 2579 Fragments arranged by size: 2579 1500 1097 411 272 248 246 118 95 38 24 Bpu10I CC'TnA_GC Cuts at: 2343 3149 3780 2343 Size: 806 631 5191 Fragments arranged by size: 5191 806 631 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2364 3071 4753 5618 5916 2364 Size: 707 1682 865 298 3076 Fragments arranged by size: 3076 1682 865 707 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2941 3200 5291 882 Size: 617 1442 259 2091 2219 Fragments arranged by size: 2219 2091 1442 617 259 BsaAI yAC'GTr Cuts at: 493 1204 3925 4419 493 Size: 711 2721 494 2702 Fragments arranged by size: 2721 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6622 6622 Size: 6628 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2488 3190 4435 4817 Size: 53 83 186 936 955 702 1245 382 Cuts at: 4817 5452 275 Size: 635 1451 Fragments arranged by size: 1451 1245 955 936 702 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5061 5459 5733 5880 6291 5061 Size: 398 274 147 411 5398 Fragments arranged by size: 5398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6403 Fragments arranged by size: 6403 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 2396 3017 3218 3380 3854 1186 Size: 1207 3 621 201 162 474 3960 Fragments arranged by size: 3960 1207 621 474 201 162 3 BseYI C'CCAG_C Cuts at: 2058 2388 2420 2940 2979 3128 3520 3621 3882 Size: 330 32 520 39 149 392 101 261 Cuts at: 3882 5831 6327 2058 Size: 1949 496 2359 Fragments arranged by size: 2359 1949 520 496 392 330 261 149 101 39 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2581 2827 3080 3167 3455 3937 1163 Size: 573 845 246 253 87 288 482 3854 Fragments arranged by size: 3854 845 573 482 288 253 246 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2067 4078 4839 5867 1540 Size: 275 186 66 2011 761 1028 2301 Fragments arranged by size: 2301 2011 1028 761 275 186 66 BsiHKAI G_wGCw'C Cuts at: 2175 2401 2711 2769 2951 3087 3846 4689 4774 Size: 226 310 58 182 136 759 843 85 Cuts at: 4774 5845 2175 Size: 1071 2958 Fragments arranged by size: 2958 1071 843 759 310 226 182 136 85 58 BsmI GAATG_Cn' Cuts at: 4139 4232 4139 Size: 93 6535 BsmBI CGTCTCn'nnnn_ Cuts at: 2711 3007 2711 Size: 296 6332 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2101 2175 2401 2416 2568 2611 Size: 399 295 16 74 226 15 152 43 Cuts at: 2611 2711 2769 2951 3087 3275 3404 3494 3846 Size: 100 58 182 136 188 129 90 352 Cuts at: 3846 3904 3947 4689 4774 5845 1391 Size: 58 43 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 352 295 226 188 182 152 136 129 100 90 85 74 58 58 43 43 16 15 BspEI T'CCGG_A Cuts at: 6291 6291 Size: 6628 BspHI T'CATG_A Cuts at: 2668 4517 6247 2668 Size: 1849 1730 3049 Fragments arranged by size: 3049 1849 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3031 3983 844 Size: 2187 952 3489 Fragments arranged by size: 3489 2187 952 BsrBI CCG'CTC Cuts at: 1394 1823 2782 2965 4515 1394 Size: 429 959 183 1550 3507 Fragments arranged by size: 3507 1550 959 429 183 BsrDI GCAATG_nn' Cuts at: 65 1248 5123 5297 65 Size: 1183 3875 174 1396 Fragments arranged by size: 3875 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3559 5272 5459 1700 Size: 110 1749 1713 187 2869 Fragments arranged by size: 2869 1749 1713 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6628 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6628 BssSI C'ACGA_G Cuts at: 4688 5700 4688 Size: 1012 5616 BstAPI GCAn_nnn'nTGC Cuts at: 794 2170 2470 3145 794 Size: 1376 300 675 4277 Fragments arranged by size: 4277 1376 675 300 BstBI TT'CG_AA Cuts at: 2007 5432 2007 Size: 3425 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6628 BstYI r'GATC_y Cuts at: 1972 2196 2455 2534 3026 3177 3625 3684 3958 Size: 224 259 79 492 151 448 59 274 Cuts at: 3958 4325 4710 4727 6179 6265 6277 6623 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 492 448 385 367 346 274 259 224 151 86 79 59 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2085 2355 2568 2889 3444 513 Size: 552 957 63 270 213 321 555 3697 Fragments arranged by size: 3697 957 555 552 321 270 213 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5889 Fragments arranged by size: 5889 683 56 BtsI GCAGTG_nn' Cuts at: 2705 2815 3333 4144 4938 4958 2705 Size: 110 518 811 794 20 4375 Fragments arranged by size: 4375 811 794 518 110 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6628 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6593 DraI TTT'AAA Cuts at: 3932 3932 Size: 6628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2884 5635 809 Size: 507 1568 2751 1802 Fragments arranged by size: 2751 1802 1568 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2221 2352 3246 Size: 54 1425 50 275 18 391 131 894 Cuts at: 3246 4075 4964 6593 8 Size: 829 889 1629 43 Fragments arranged by size: 1629 1425 894 889 829 391 275 131 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4075 1537 Size: 275 2263 4090 Fragments arranged by size: 4090 2263 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 3579 3731 4558 1353 Size: 711 1515 152 827 3423 Fragments arranged by size: 3423 1515 827 711 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2932 3212 5215 5589 5735 387 Size: 1064 138 1343 280 2003 374 146 1280 Fragments arranged by size: 2003 1343 1280 1064 374 280 146 138 EcoICRI GAG'CTC Cuts at: 2399 2709 3085 3844 2399 Size: 310 376 759 5183 Fragments arranged by size: 5183 759 376 310 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2582 2854 2972 2978 3383 3478 Size: 393 704 246 272 118 6 405 95 Cuts at: 3478 3516 3755 3764 3788 4691 5288 6075 6343 Size: 38 239 9 24 903 597 787 268 Cuts at: 6343 1239 Size: 1524 Fragments arranged by size: 1524 903 787 704 597 405 393 272 268 246 239 118 95 38 24 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 2957 2999 3725 3836 2957 Size: 42 726 111 5749 Fragments arranged by size: 5749 726 111 42 EcoO109I rG'GnC_Cy Cuts at: 2098 2851 2992 3091 3583 3853 3900 3901 2098 Size: 753 141 99 492 270 47 1 4825 Fragments arranged by size: 4825 753 492 270 141 99 47 1 EcoRI G'AATT_C Cuts at: 3937 4315 3937 Size: 378 6250 EcoRV GAT'ATC Cuts at: 2838 2838 Size: 6628 FspI TGC'GCA Cuts at: 2117 5134 2117 Size: 3017 3611 HaeII r_GCGC'y Cuts at: 1678 2491 2986 3067 3193 3509 5775 1678 Size: 813 495 81 126 316 2266 2531 Fragments arranged by size: 2531 2266 813 495 316 126 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2783 2815 5269 5301 5343 Size: 32 135 32 1211 32 2454 32 42 Cuts at: 5343 5375 1373 Size: 32 2626 Fragments arranged by size: 2626 2454 1211 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3972 4218 669 Size: 988 281 2034 246 3079 Fragments arranged by size: 3079 2034 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6628 HpaI GTT'AAC Cuts at: 4218 4218 Size: 6628 KasI G'GCGC_C Cuts at: 2487 3189 2487 Size: 702 5926 KpnI G_GTAC'C Cuts at: 3953 3953 Size: 6628 MfeI C'AATT_G Cuts at: 4227 4227 Size: 6628 MluI A'CGCG_T Cuts at: 3016 6504 3016 Size: 3488 3140 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2228 5742 5926 1305 Size: 923 3514 184 2007 Fragments arranged by size: 3514 2007 923 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2223 10 Size: 54 1425 343 391 4415 Fragments arranged by size: 4415 1425 391 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2354 4586 4945 5104 518 Size: 648 1188 2232 359 159 2042 Fragments arranged by size: 2232 2042 1188 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2467 2944 3128 3175 3196 3469 3607 4044 Size: 861 477 184 47 21 273 138 437 Cuts at: 4044 4721 5869 6114 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 861 677 477 437 273 245 184 138 47 21 NaeI GCC'GGC Cuts at: 3561 3561 Size: 6628 NarI GG'CG_CC Cuts at: 2488 3190 2488 Size: 702 5926 NcoI C'CATG_G Cuts at: 513 1065 2022 2355 2889 513 Size: 552 957 333 534 4252 Fragments arranged by size: 4252 957 552 534 333 NdeI CA'TA_TG Cuts at: 387 387 Size: 6628 NgoMIV G'CCGG_C Cuts at: 3559 3559 Size: 6628 NheI G'CTAG_C Cuts at: 1052 2029 3286 1052 Size: 977 1257 4394 Fragments arranged by size: 4394 1257 977 NotI GC'GGCC_GC Cuts at: 4075 4075 Size: 6628 NspI r_CATG'y Cuts at: 1132 1229 2171 3986 5531 1132 Size: 97 942 1815 1545 2229 Fragments arranged by size: 2229 1815 1545 942 97 PasI CC'CwG_GG Cuts at: 1348 3895 1348 Size: 2547 4081 PciI A'CATG_T Cuts at: 5527 5527 Size: 6628 PflMI CCAn_nnn'nTGG Cuts at: 1108 2314 2752 2995 3199 3520 1108 Size: 1206 438 243 204 321 4216 Fragments arranged by size: 4216 1206 438 321 243 204 PfoI T'CCnGG_A Cuts at: 2295 2904 2295 Size: 609 6019 PmeI GTTT'AAAC Cuts at: 3932 3932 Size: 6628 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4697 4729 6210 6242 4697 Size: 32 1481 32 5083 Fragments arranged by size: 5083 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3583 3583 Size: 6628 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6628 PsiI TTA'TAA Cuts at: 4198 4198 Size: 6628 PspOMI G'GGCC_C Cuts at: 2097 2412 2607 3900 2097 Size: 315 195 1293 4825 Fragments arranged by size: 4825 1293 315 195 PstI C_TGCA'G Cuts at: 830 2475 2496 2622 2779 3120 3600 3684 3789 Size: 1645 21 126 157 341 480 84 105 Cuts at: 3789 3909 3980 830 Size: 120 71 3478 Fragments arranged by size: 3478 1645 480 341 157 126 120 105 84 71 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6628 PvuII CAG'CTG Cuts at: 3128 3196 3469 3128 Size: 68 273 6287 Fragments arranged by size: 6287 273 68 SacI G_AGCT'C Cuts at: 2401 2711 3087 3846 2401 Size: 310 376 759 5183 Fragments arranged by size: 5183 759 376 310 SalI G'TCGA_C Cuts at: 1936 3970 1936 Size: 2034 4594 SbfI CC_TGCA'GG Cuts at: 2496 3600 3980 2496 Size: 1104 380 5144 Fragments arranged by size: 5144 1104 380 ScaI AGT'ACT Cuts at: 4876 6588 4876 Size: 1712 4916 SfcI C'TryA_G Cuts at: 826 1045 2471 2492 2618 2775 3116 3158 3596 Size: 219 1426 21 126 157 341 42 438 Cuts at: 3596 3680 3785 3905 3976 5111 5792 5983 826 Size: 84 105 120 71 1135 681 191 1471 Fragments arranged by size: 1471 1426 1135 681 438 341 219 191 157 126 120 105 84 71 42 21 SfiI GGCCn_nnn'nGGCC Cuts at: 3899 3899 Size: 6628 SfoI GGC'GCC Cuts at: 2489 3191 2489 Size: 702 5926 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6628 SmaI CCC'GGG Cuts at: 1872 2096 3276 3955 1872 Size: 224 1180 679 4545 Fragments arranged by size: 4545 1180 679 224 SmlI C'TyrA_G Cuts at: 820 1017 1943 2379 3086 4732 5633 5895 820 Size: 197 926 436 707 1646 901 262 1553 Fragments arranged by size: 1646 1553 926 901 707 436 262 197 SnaBI TAC'GTA Cuts at: 493 3925 4419 493 Size: 3432 494 2702 Fragments arranged by size: 3432 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6628 SphI G_CATG'C Cuts at: 1132 3986 1132 Size: 2854 3774 SrfI GCCC'GGGC Cuts at: 2096 2096 Size: 6628 SspI AAT'ATT Cuts at: 5 52 4552 5 Size: 47 4500 2081 Fragments arranged by size: 4500 2081 47 StuI AGG'CCT Cuts at: 1517 3542 3660 1517 Size: 2025 118 4485 Fragments arranged by size: 4485 2025 118 StyI C'CwwG_G Cuts at: 513 1065 2022 2355 2760 2889 3485 4067 513 Size: 552 957 333 405 129 596 582 3074 Fragments arranged by size: 3074 957 596 582 552 405 333 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2402 2787 2915 3683 4655 4672 4825 Size: 1255 822 385 128 768 972 17 153 Cuts at: 4825 5010 5455 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 972 822 768 445 385 185 153 128 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4874 6586 96 Size: 274 80 33 51 1443 2897 1712 138 Fragments arranged by size: 2897 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2911 2943 3464 3496 6588 6620 2911 Size: 32 521 32 3092 32 2919 Fragments arranged by size: 3092 2919 521 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6628 XbaI T'CTAG_A Cuts at: 3964 3964 Size: 6628 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6628 XmaI C'CCGG_G Cuts at: 1870 2094 3274 3953 1870 Size: 224 1180 679 4545 Fragments arranged by size: 4545 1180 679 224 XmnI GAAnn'nnTTC Cuts at: 4757 4757 Size: 6628 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4436 276 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SwaI XcmI