Restriction map for
pFN21ASDA0841
(Linear) (Six-base) MAPSORT of: KIAA0841.seq Check: 3575 from: 1 to: 1899
KIAA0841.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1008 1899
Size: 1008 891
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1732 1899
Size: 955 777 167
Fragments arranged by size:
955 777 167
AfeI AGC'GCT
Cuts at: 0 1484 1899
Size: 1484 415
AflIII A'CryG_T
Cuts at: 0 993 1899
Size: 993 906
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 767 1402 1899
Size: 767 635 497
Fragments arranged by size:
767 635 497
AlwNI CAG_nnn'CTG
Cuts at: 0 291 447 594 681 858 972 1023 1092
Size: 291 156 147 87 177 114 51 69
Cuts at: 1092 1122 1653 1710 1743 1833 1890 1899
Size: 30 531 57 33 90 57 9
Fragments arranged by size:
531 291 177 156 147 114 90 87
69 57 57 51 33 30 9
ApaI G_GGCC'C
Cuts at: 0 78 393 588 1881 1899
Size: 78 315 195 1293 18
Fragments arranged by size:
1293 315 195 78 18
ApaLI G'TGCA_C
Cuts at: 0 148 1899
Size: 148 1751
AvaI C'yCGr_G
Cuts at: 0 71 1251 1899
Size: 71 1180 648
Fragments arranged by size:
1180 648 71
BamHI G'GATC_C
Cuts at: 0 511 1003 1154 1899
Size: 511 492 151 745
Fragments arranged by size:
745 511 492 151
BanI G'GyrC_C
Cuts at: 0 57 464 1166 1308 1899
Size: 57 407 702 142 591
Fragments arranged by size:
702 591 407 142 57
BanII G_rGCy'C
Cuts at: 0 78 378 393 545 588 688 1064 1252
Size: 78 300 15 152 43 100 376 188
Cuts at: 1252 1471 1823 1881 1899
Size: 219 352 58 18
Fragments arranged by size:
376 352 300 219 188 152 100 78
58 43 18 15
BbeI G_GCGC'C
Cuts at: 0 468 1170 1899
Size: 468 702 729
Fragments arranged by size:
729 702 468
BbsI GAAGACnn'nnnn_
Cuts at: 0 417 1624 1899
Size: 417 1207 275
Fragments arranged by size:
1207 417 275
BbvCI CC'TCA_GC
Cuts at: 0 1126 1899
Size: 1126 773
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 554 588 1899
Size: 554 34 1311
Fragments arranged by size:
1311 554 34
BglI GCCn_nnn'nGGC
Cuts at: 0 968 1848 1876 1899
Size: 968 880 28 23
Fragments arranged by size:
968 880 28 23
BlpI GC'TnA_GC
Cuts at: 0 682 1486 1899
Size: 682 804 413
Fragments arranged by size:
804 682 413
Bme1580I G_kGCm'C
Cuts at: 0 62 78 152 393 588 1381 1881 1899
Size: 62 16 74 241 195 793 500 18
Fragments arranged by size:
793 500 241 195 74 62 18 16
BmgBI CAC'GTC
Cuts at: 0 346 521 726 1899
Size: 346 175 205 1173
Fragments arranged by size:
1173 346 205 175
BmrI ACTGGGnnnn_n'
Cuts at: 0 37 582 1039 1839 1899
Size: 37 545 457 800 60
Fragments arranged by size:
800 545 457 60 37
BmtI G_CTAG'C
Cuts at: 0 10 1267 1899
Size: 10 1257 632
Fragments arranged by size:
1257 632 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 397 1899
Size: 397 1502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 559 831 949 1360 1455 1493 1741
Size: 313 246 272 118 411 95 38 248
Cuts at: 1741 1765 1899
Size: 24 134
Fragments arranged by size:
411 313 272 248 246 134 118 95
38 24
Bpu10I CC'TnA_GC
Cuts at: 0 320 1126 1757 1899
Size: 320 806 631 142
Fragments arranged by size:
806 631 320 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 1048 1899
Size: 341 707 851
Fragments arranged by size:
851 707 341
BsaI GGTCTCn'nnnn_
Cuts at: 0 918 1177 1899
Size: 918 259 722
Fragments arranged by size:
918 722 259
BsaHI Gr'CG_yC
Cuts at: 0 465 1167 1899
Size: 465 702 732
Fragments arranged by size:
732 702 465
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 373 994 1195 1357 1831 1899
Size: 370 3 621 201 162 474 68
Fragments arranged by size:
621 474 370 201 162 68 3
BseYI C'CCAG_C
Cuts at: 0 35 365 397 917 956 1105 1497 1598
Size: 35 330 32 520 39 149 392 101
Cuts at: 1598 1859 1899
Size: 261 40
Fragments arranged by size:
520 392 330 261 149 101 40 39
35 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 804 1057 1144 1432 1899
Size: 558 246 253 87 288 467
Fragments arranged by size:
558 467 288 253 246 87
BsiEI CG_ry'CG
Cuts at: 0 44 1899
Size: 44 1855
BsiHKAI G_wGCw'C
Cuts at: 0 152 378 688 746 928 1064 1823 1899
Size: 152 226 310 58 182 136 759 76
Fragments arranged by size:
759 310 226 182 152 136 76 58
BsmBI CGTCTCn'nnnn_
Cuts at: 0 688 984 1899
Size: 688 296 915
Fragments arranged by size:
915 688 296
Bsp1286I G_dGCh'C
Cuts at: 0 62 78 152 378 393 545 588 688
Size: 62 16 74 226 15 152 43 100
Cuts at: 688 746 928 1064 1252 1381 1471 1823 1881
Size: 58 182 136 188 129 90 352 58
Cuts at: 1881 1899
Size: 18
Fragments arranged by size:
352 226 188 182 152 136 129 100
90 74 62 58 58 43 18 16
15
BspHI T'CATG_A
Cuts at: 0 645 1899
Size: 645 1254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1008 1899
Size: 1008 891
BsrBI CCG'CTC
Cuts at: 0 759 942 1899
Size: 759 183 957
Fragments arranged by size:
957 759 183
BsrFI r'CCGG_y
Cuts at: 0 1536 1899
Size: 1536 363
BstAPI GCAn_nnn'nTGC
Cuts at: 0 147 447 1122 1899
Size: 147 300 675 777
Fragments arranged by size:
777 675 300 147
BstYI r'GATC_y
Cuts at: 0 173 432 511 1003 1154 1602 1661 1899
Size: 173 259 79 492 151 448 59 238
Fragments arranged by size:
492 448 259 238 173 151 79 59
BtgI C'CryG_G
Cuts at: 0 62 332 545 866 1421 1899
Size: 62 270 213 321 555 478
Fragments arranged by size:
555 478 321 270 213 62
BtsI GCAGTG_nn'
Cuts at: 0 682 792 1310 1899
Size: 682 110 518 589
Fragments arranged by size:
682 589 518 110
DrdI GACnn_nn'nnGTC
Cuts at: 0 861 1899
Size: 861 1038
EaeI y'GGCC_r
Cuts at: 0 198 329 1223 1899
Size: 198 131 894 676
Fragments arranged by size:
894 676 198 131
EarI CTCTTCn'nnn_
Cuts at: 0 41 1556 1708 1899
Size: 41 1515 152 191
Fragments arranged by size:
1515 191 152 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 909 1189 1899
Size: 909 280 710
Fragments arranged by size:
909 710 280
EcoICRI GAG'CTC
Cuts at: 0 376 686 1062 1821 1899
Size: 376 310 376 759 78
Fragments arranged by size:
759 376 376 310 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 559 831 949 955 1360 1455 1493
Size: 313 246 272 118 6 405 95 38
Cuts at: 1493 1732 1741 1765 1899
Size: 239 9 24 134
Fragments arranged by size:
405 313 272 246 239 134 118 95
38 24 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 934 976 1702 1813 1899
Size: 934 42 726 111 86
Fragments arranged by size:
934 726 111 86 42
EcoO109I rG'GnC_Cy
Cuts at: 0 75 828 969 1068 1560 1830 1877 1878
Size: 75 753 141 99 492 270 47 1
Cuts at: 1878 1899
Size: 21
Fragments arranged by size:
753 492 270 141 99 75 47 21
1
EcoRV GAT'ATC
Cuts at: 0 815 1899
Size: 815 1084
FspI TGC'GCA
Cuts at: 0 94 1899
Size: 94 1805
HaeII r_GCGC'y
Cuts at: 0 468 963 1044 1170 1486 1899
Size: 468 495 81 126 316 413
Fragments arranged by size:
495 468 413 316 126 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 760 792 1899
Size: 760 32 1107
Fragments arranged by size:
1107 760 32
KasI G'GCGC_C
Cuts at: 0 464 1166 1899
Size: 464 702 733
Fragments arranged by size:
733 702 464
MluI A'CGCG_T
Cuts at: 0 993 1899
Size: 993 906
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1899
Size: 205 1694
MscI TGG'CCA
Cuts at: 0 200 1899
Size: 200 1699
MslI CAynn'nnrTG
Cuts at: 0 331 1899
Size: 331 1568
MspA1I CmG'CkG
Cuts at: 0 444 921 1105 1152 1173 1446 1584 1899
Size: 444 477 184 47 21 273 138 315
Fragments arranged by size:
477 444 315 273 184 138 47 21
NaeI GCC'GGC
Cuts at: 0 1538 1899
Size: 1538 361
NarI GG'CG_CC
Cuts at: 0 465 1167 1899
Size: 465 702 732
Fragments arranged by size:
732 702 465
NcoI C'CATG_G
Cuts at: 0 332 866 1899
Size: 332 534 1033
Fragments arranged by size:
1033 534 332
NgoMIV G'CCGG_C
Cuts at: 0 1536 1899
Size: 1536 363
NheI G'CTAG_C
Cuts at: 0 6 1263 1899
Size: 6 1257 636
Fragments arranged by size:
1257 636 6
NspI r_CATG'y
Cuts at: 0 148 1899
Size: 148 1751
PasI CC'CwG_GG
Cuts at: 0 1872 1899
Size: 1872 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 291 729 972 1176 1497 1899
Size: 291 438 243 204 321 402
Fragments arranged by size:
438 402 321 291 243 204
PfoI T'CCnGG_A
Cuts at: 0 272 881 1899
Size: 272 609 1018
Fragments arranged by size:
1018 609 272
PpuMI rG'GwC_Cy
Cuts at: 0 1560 1899
Size: 1560 339
PspOMI G'GGCC_C
Cuts at: 0 74 389 584 1877 1899
Size: 74 315 195 1293 22
Fragments arranged by size:
1293 315 195 74 22
PstI C_TGCA'G
Cuts at: 0 452 473 599 756 1097 1577 1661 1766
Size: 452 21 126 157 341 480 84 105
Cuts at: 1766 1886 1899
Size: 120 13
Fragments arranged by size:
480 452 341 157 126 120 105 84
21 13
PvuII CAG'CTG
Cuts at: 0 1105 1173 1446 1899
Size: 1105 68 273 453
Fragments arranged by size:
1105 453 273 68
SacI G_AGCT'C
Cuts at: 0 378 688 1064 1823 1899
Size: 378 310 376 759 76
Fragments arranged by size:
759 378 376 310 76
SbfI CC_TGCA'GG
Cuts at: 0 473 1577 1899
Size: 473 1104 322
Fragments arranged by size:
1104 473 322
SfcI C'TryA_G
Cuts at: 0 448 469 595 752 1093 1135 1573 1657
Size: 448 21 126 157 341 42 438 84
Cuts at: 1657 1762 1882 1899
Size: 105 120 17
Fragments arranged by size:
448 438 341 157 126 120 105 84
42 21 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1876 1899
Size: 1876 23
SfoI GGC'GCC
Cuts at: 0 466 1168 1899
Size: 466 702 731
Fragments arranged by size:
731 702 466
SmaI CCC'GGG
Cuts at: 0 73 1253 1899
Size: 73 1180 646
Fragments arranged by size:
1180 646 73
SmlI C'TyrA_G
Cuts at: 0 356 1063 1899
Size: 356 707 836
Fragments arranged by size:
836 707 356
SrfI GCCC'GGGC
Cuts at: 0 73 1899
Size: 73 1826
StuI AGG'CCT
Cuts at: 0 1519 1637 1899
Size: 1519 118 262
Fragments arranged by size:
1519 262 118
StyI C'CwwG_G
Cuts at: 0 332 737 866 1462 1899
Size: 332 405 129 596 437
Fragments arranged by size:
596 437 405 332 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 379 764 892 1660 1899
Size: 379 385 128 768 239
Fragments arranged by size:
768 385 379 239 128
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 888 920 1441 1473 1899
Size: 888 32 521 32 426
Fragments arranged by size:
888 521 426 32 32
XmaI C'CCGG_G
Cuts at: 0 71 1251 1899
Size: 71 1180 648
Fragments arranged by size:
1180 648 71
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI
BstYI BtgI BtsI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SbfI SfcI SfiI SfoI SmaI
SmlI SrfI StuI StyI TaqII TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AloI ApoI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BglII BsaAI BsaBI BsaWI BsaXI BsiWI
BsmI BspEI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EagI EcoRI FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI NdeI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI ScaI SexAI SgfI
SgrAI SnaBI SpeI SphI SspI SwaI TatI Tth111I
XbaI XcmI XhoI XmnI ZraI