(Circular) (Six-base) MAPSORT of: pFN21ASDA0832.seq Check: 445 from: 1 to: 6103 pFN21ASDA0832 6103 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3913 278 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2964 3396 3446 1937 Size: 1027 432 50 4594 Fragments arranged by size: 4594 1027 432 50 Acc65I G'GTAC_C Cuts at: 2433 3424 2433 Size: 991 5112 AclI AA'CG_TT Cuts at: 1563 4230 4603 1563 Size: 2667 373 3063 Fragments arranged by size: 3063 2667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2190 3179 4166 5550 5818 1632 Size: 558 989 987 1384 268 1917 Fragments arranged by size: 1917 1384 989 987 558 268 AfeI AGC'GCT Cuts at: 3216 3216 Size: 6103 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5906 AflIII A'CryG_T Cuts at: 1203 5002 5979 1203 Size: 3799 977 1327 Fragments arranged by size: 3799 1327 977 AgeI A'CCGG_T Cuts at: 2967 4934 2967 Size: 1967 4136 AhdI GACnn_n'nnGTC Cuts at: 4832 4832 Size: 6103 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2212 2244 2212 Size: 32 6071 AlwNI CAG_nnn'CTG Cuts at: 3846 5418 3846 Size: 1572 4531 ApaLI G'TGCA_C Cuts at: 4160 5316 4160 Size: 1156 4947 ApoI r'AATT_y Cuts at: 1493 2943 3116 3412 3638 3790 5899 1493 Size: 1450 173 296 226 152 2109 1697 Fragments arranged by size: 2109 1697 1450 296 226 173 152 AseI AT'TA_AT Cuts at: 160 1032 4657 160 Size: 872 3625 1606 Fragments arranged by size: 3625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 3428 1870 Size: 73 1485 4545 Fragments arranged by size: 4545 1485 73 BamHI G'GATC_C Cuts at: 3433 3800 3433 Size: 367 5736 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2433 3424 4879 618 Size: 325 217 627 646 991 1455 1842 Fragments arranged by size: 1842 1455 991 646 627 325 217 BanII G_rGCy'C Cuts at: 1391 3422 1391 Size: 2031 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3030 3255 928 Size: 971 1131 225 3776 Fragments arranged by size: 3776 1131 971 225 BbvCI CC'TCA_GC Cuts at: 2694 2694 Size: 6103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4294 4328 1903 Size: 34 2357 34 3678 Fragments arranged by size: 3678 2357 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3995 5205 3995 Size: 1210 4893 BclI T'GATC_A Cuts at: 1548 1548 Size: 6103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2199 2468 4714 Size: 107 122 71 631 605 527 269 2246 Cuts at: 4714 136 Size: 1525 Fragments arranged by size: 2246 1525 631 605 527 269 122 107 71 BglII A'GATC_T Cuts at: 2766 6098 2766 Size: 3332 2771 BlpI GC'TnA_GC Cuts at: 3520 3520 Size: 6103 Bme1580I G_kGCm'C Cuts at: 1790 4164 5320 1790 Size: 2374 1156 2573 Fragments arranged by size: 2573 2374 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3834 4792 442 Size: 416 53 83 399 24 2417 958 1753 Fragments arranged by size: 2417 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2320 4763 1239 Size: 1081 2443 2579 Fragments arranged by size: 2579 2443 1081 Bpu10I CC'TnA_GC Cuts at: 2694 2694 Size: 6103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4228 5093 5391 4228 Size: 865 298 4940 Fragments arranged by size: 4940 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2976 4766 882 Size: 617 1477 1790 2219 Fragments arranged by size: 2219 1790 1477 617 BsaAI yAC'GTr Cuts at: 493 1204 2633 3400 3894 493 Size: 711 1429 767 494 2702 Fragments arranged by size: 2702 1429 767 711 494 BsaBI GATnn'nnATC Cuts at: 6097 6097 Size: 6103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2211 3910 4292 4927 Size: 53 83 186 936 678 1699 382 635 Cuts at: 4927 275 Size: 1451 Fragments arranged by size: 1699 1451 936 678 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2758 2967 4536 4934 5208 5355 5766 2758 Size: 209 1569 398 274 147 411 3095 Fragments arranged by size: 3095 1569 411 398 274 209 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2212 2242 3273 3303 1178 Size: 30 165 30 809 30 1031 30 3978 Fragments arranged by size: 3978 1031 809 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6103 BseYI C'CCAG_C Cuts at: 2158 5306 5802 2158 Size: 3148 496 2459 Fragments arranged by size: 3148 2459 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 3338 1163 Size: 573 312 1290 3928 Fragments arranged by size: 3928 1290 573 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2842 3553 4314 5342 1540 Size: 275 186 841 711 761 1028 2301 Fragments arranged by size: 2301 1028 841 761 711 275 186 BsiHKAI G_wGCw'C Cuts at: 4164 4249 5320 4164 Size: 85 1071 4947 Fragments arranged by size: 4947 1071 85 BsmI GAATG_Cn' Cuts at: 2471 3614 3707 2471 Size: 1143 93 4867 Fragments arranged by size: 4867 1143 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3422 4164 4249 5320 1391 Size: 399 1632 742 85 1071 2174 Fragments arranged by size: 2174 1632 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5766 5766 Size: 6103 BspHI T'CATG_A Cuts at: 2577 3992 5722 2577 Size: 1415 1730 2958 Fragments arranged by size: 2958 1730 1415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3458 844 Size: 2614 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3990 1394 Size: 429 2167 3507 Fragments arranged by size: 3507 2167 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4598 4772 65 Size: 1183 3350 174 1396 Fragments arranged by size: 3350 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2967 4747 4934 1700 Size: 110 1157 1780 187 2869 Fragments arranged by size: 2869 1780 1157 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6103 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6103 BssSI C'ACGA_G Cuts at: 4163 5175 4163 Size: 1012 5091 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6103 BstBI TT'CG_AA Cuts at: 2007 4907 2007 Size: 2900 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6103 BstXI CCAn_nnnn'nTGG Cuts at: 2195 2195 Size: 6103 BstYI r'GATC_y Cuts at: 1972 2353 2766 3433 3800 4185 4202 5654 5740 Size: 381 413 667 367 385 17 1452 86 Cuts at: 5740 5752 6098 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 667 413 385 381 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2965 2965 Size: 6103 Bsu36I CC'TnA_GG Cuts at: 3293 3293 Size: 6103 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4594 Fragments arranged by size: 4594 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2407 487 Size: 683 56 1181 4183 Fragments arranged by size: 4183 1181 683 56 BtsI GCAGTG_nn' Cuts at: 3281 3619 4413 4433 3281 Size: 338 794 20 4951 Fragments arranged by size: 4951 794 338 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 2589 3407 2589 Size: 818 5285 DraIII CAC_nnn'GTG Cuts at: 2828 2828 Size: 6103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2789 5110 809 Size: 507 1473 2321 1802 Fragments arranged by size: 2321 1802 1473 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2242 2835 3235 Size: 54 1425 50 275 18 412 593 400 Cuts at: 3235 3550 4439 6068 8 Size: 315 889 1629 43 Fragments arranged by size: 1629 1425 889 593 412 400 315 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3550 1537 Size: 275 1738 4090 Fragments arranged by size: 4090 1738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2070 2209 4033 1353 Size: 717 139 1824 3423 Fragments arranged by size: 3423 1824 717 139 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2921 4690 5064 5210 387 Size: 1064 138 1332 1769 374 146 1280 Fragments arranged by size: 1769 1332 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2320 3179 4166 4763 5550 5818 Size: 393 558 130 859 987 597 787 268 Cuts at: 5818 1239 Size: 1524 Fragments arranged by size: 1524 987 859 787 597 558 393 268 130 EcoRI G'AATT_C Cuts at: 3412 3790 3412 Size: 378 5725 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2491 2491 Size: 6103 FspI TGC'GCA Cuts at: 4609 4609 Size: 6103 HaeII r_GCGC'y Cuts at: 1678 3218 5250 1678 Size: 1540 2032 2531 Fragments arranged by size: 2531 2032 1540 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2318 2350 4744 4776 4818 Size: 32 135 32 746 32 2394 32 42 Cuts at: 4818 4850 1373 Size: 32 2626 Fragments arranged by size: 2626 2394 746 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2182 3447 3693 669 Size: 988 281 244 1265 246 3079 Fragments arranged by size: 3079 1265 988 281 246 244 HindIII A'AGCT_T Cuts at: 748 3191 748 Size: 2443 3660 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KpnI G_GTAC'C Cuts at: 2437 3428 2437 Size: 991 5112 MfeI C'AATT_G Cuts at: 3702 3702 Size: 6103 MluI A'CGCG_T Cuts at: 5979 5979 Size: 6103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3363 5217 5401 1305 Size: 2058 1854 184 2007 Fragments arranged by size: 2058 2007 1854 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2244 3237 10 Size: 54 1425 343 412 993 2876 Fragments arranged by size: 2876 1425 993 412 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4061 4420 4579 518 Size: 648 2895 359 159 2042 Fragments arranged by size: 2895 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2124 2511 2698 3076 3519 4196 5344 5589 Size: 518 387 187 378 443 677 1148 245 Cuts at: 5589 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 518 443 387 378 245 187 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4594 Fragments arranged by size: 4594 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6103 NotI GC'GGCC_GC Cuts at: 3550 3550 Size: 6103 NspI r_CATG'y Cuts at: 1132 1229 2380 2602 3461 5006 1132 Size: 97 1151 222 859 1545 2229 Fragments arranged by size: 2229 1545 1151 859 222 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6103 PciI A'CATG_T Cuts at: 5002 5002 Size: 6103 PflMI CCAn_nnn'nTGG Cuts at: 1108 3244 1108 Size: 2136 3967 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2212 2244 4172 4204 5685 5717 2212 Size: 32 1928 32 1481 32 2598 Fragments arranged by size: 2598 1928 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3255 1613 Size: 1642 4461 PsiI TTA'TAA Cuts at: 3673 3673 Size: 6103 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2666 2698 2666 Size: 32 6071 PstI C_TGCA'G Cuts at: 830 2090 2928 3075 3222 3455 830 Size: 1260 838 147 147 233 3478 Fragments arranged by size: 3478 1260 838 233 147 147 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 PvuII CAG'CTG Cuts at: 2124 2511 2698 3076 2124 Size: 387 187 378 5151 Fragments arranged by size: 5151 387 378 187 SalI G'TCGA_C Cuts at: 1936 3445 1936 Size: 1509 4594 SapI GCTCTTCn'nnn_ Cuts at: 2070 2209 2070 Size: 139 5964 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 4351 6063 4351 Size: 1712 4391 SfcI C'TryA_G Cuts at: 826 1045 2086 2224 2924 3071 3135 3218 3451 Size: 219 1041 138 700 147 64 83 233 Cuts at: 3451 4586 5267 5458 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1041 700 681 233 219 191 147 138 83 64 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6103 SmaI CCC'GGG Cuts at: 1872 3430 1872 Size: 1558 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4207 5108 5370 820 Size: 197 926 2264 901 262 1553 Fragments arranged by size: 2264 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3400 3894 493 Size: 2907 494 2702 Fragments arranged by size: 2907 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6103 SphI G_CATG'C Cuts at: 1132 2602 3461 1132 Size: 1470 859 3774 Fragments arranged by size: 3774 1470 859 SspI AAT'ATT Cuts at: 5 52 4027 5 Size: 47 3975 2081 Fragments arranged by size: 3975 2081 47 StuI AGG'CCT Cuts at: 1517 3045 1517 Size: 1528 4575 StyI C'CwwG_G Cuts at: 513 1065 2022 2953 3309 3390 3542 513 Size: 552 957 931 356 81 152 3074 Fragments arranged by size: 3074 957 931 552 356 152 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2856 4130 4147 4300 4485 4930 325 Size: 1255 1276 1274 17 153 185 445 1498 Fragments arranged by size: 1498 1276 1274 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2649 4349 6061 Size: 274 80 33 51 1443 672 1700 1712 Cuts at: 6061 96 Size: 138 Fragments arranged by size: 1712 1700 1443 672 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2102 2134 3273 3305 6063 6095 2102 Size: 32 1139 32 2758 32 2110 Fragments arranged by size: 2758 2110 1139 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2177 1316 Size: 861 5242 XbaI T'CTAG_A Cuts at: 3439 3439 Size: 6103 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2192 2195 2899 2192 Size: 3 704 5396 Fragments arranged by size: 5396 704 3 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6103 XmaI C'CCGG_G Cuts at: 1870 3428 1870 Size: 1558 4545 XmnI GAAnn'nnTTC Cuts at: 2079 3183 4232 2079 Size: 1104 1049 3950 Fragments arranged by size: 3950 1104 1049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3911 276 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI