Restriction map for
pFN21ASDA0821
(Linear) (Six-base) MAPSORT of: KIAA0821.seq Check: 674 from: 1 to: 4422
KIAA0821.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1920 3560 4422
Size: 1920 1640 862
Fragments arranged by size:
1920 1640 862
AatII G_ACGT'C
Cuts at: 0 164 1709 1961 4422
Size: 164 1545 252 2461
Fragments arranged by size:
2461 1545 252 164
AccI GT'mk_AC
Cuts at: 0 653 1545 2449 4422
Size: 653 892 904 1973
Fragments arranged by size:
1973 904 892 653
Acc65I G'GTAC_C
Cuts at: 0 3535 4422
Size: 3535 887
AfeI AGC'GCT
Cuts at: 0 937 4422
Size: 937 3485
AflIII A'CryG_T
Cuts at: 0 1416 1418 2494 4422
Size: 1416 2 1076 1928
Fragments arranged by size:
1928 1416 1076 2
AleI CACnn'nnGTG
Cuts at: 0 282 2593 4422
Size: 282 2311 1829
Fragments arranged by size:
2311 1829 282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3349 4422
Size: 3349 1073
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2071 2103 4422
Size: 2071 32 2319
Fragments arranged by size:
2319 2071 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1592 1740 1770 2082 4422
Size: 1592 148 30 312 2340
Fragments arranged by size:
2340 1592 312 148 30
ApaI G_GGCC'C
Cuts at: 0 1579 3796 3907 3974 4047 4214 4385 4422
Size: 1579 2217 111 67 73 167 171 37
Fragments arranged by size:
2217 1579 171 167 111 73 67 37
ApaLI G'TGCA_C
Cuts at: 0 1933 2836 3310 3865 4422
Size: 1933 903 474 555 557
Fragments arranged by size:
1933 903 557 555 474
ApoI r'AATT_y
Cuts at: 0 3443 4422
Size: 3443 979
AvaI C'yCGr_G
Cuts at: 0 1445 1520 1571 1682 2106 2256 3367 3781
Size: 1445 75 51 111 424 150 1111 414
Cuts at: 3781 3806 3973 4040 4422
Size: 25 167 67 382
Fragments arranged by size:
1445 1111 424 414 382 167 150 111
75 67 51 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3519 3552 4422
Size: 3519 33 870
Fragments arranged by size:
3519 870 33
BamHI G'GATC_C
Cuts at: 0 536 3382 4422
Size: 536 2846 1040
Fragments arranged by size:
2846 1040 536
BanI G'GyrC_C
Cuts at: 0 146 661 1308 1333 1972 2272 2968 3535
Size: 146 515 647 25 639 300 696 567
Cuts at: 3535 3586 4105 4422
Size: 51 519 317
Fragments arranged by size:
696 647 639 567 519 515 317 300
146 51 25
BanII G_rGCy'C
Cuts at: 0 89 449 1433 1445 1562 1579 1690 3796
Size: 89 360 984 12 117 17 111 2106
Cuts at: 3796 3907 3923 3974 4047 4214 4385 4422
Size: 111 16 51 73 167 171 37
Fragments arranged by size:
2106 984 360 171 167 117 111 111
89 73 51 37 17 16 12
BbeI G_GCGC'C
Cuts at: 0 1976 2276 4109 4422
Size: 1976 300 1833 313
Fragments arranged by size:
1976 1833 313 300
BbsI GAAGACnn'nnnn_
Cuts at: 0 402 660 2172 2328 3264 3642 4422
Size: 402 258 1512 156 936 378 780
Fragments arranged by size:
1512 936 780 402 378 258 156
BbvCI CC'TCA_GC
Cuts at: 0 1231 1585 3396 4422
Size: 1231 354 1811 1026
Fragments arranged by size:
1811 1231 1026 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1186 1220 4422
Size: 1186 34 3202
Fragments arranged by size:
3202 1186 34
BclI T'GATC_A
Cuts at: 0 3848 4422
Size: 3848 574
BglI GCCn_nnn'nGGC
Cuts at: 0 2261 2270 2831 3829 4162 4294 4346 4422
Size: 2261 9 561 998 333 132 52 76
Fragments arranged by size:
2261 998 561 333 132 76 52 9
BglII A'GATC_T
Cuts at: 0 2546 4422
Size: 2546 1876
BlpI GC'TnA_GC
Cuts at: 0 2717 4422
Size: 2717 1705
Bme1580I G_kGCm'C
Cuts at: 0 151 421 913 1311 1579 1937 2840 3314
Size: 151 270 492 398 268 358 903 474
Cuts at: 3314 3376 3589 3796 3869 3907 3974 4047 4214
Size: 62 213 207 73 38 67 73 167
Cuts at: 4214 4385 4422
Size: 171 37
Fragments arranged by size:
903 492 474 398 358 270 268 213
207 171 167 151 73 73 67 62
38 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 294 1196 1321 1372 4041 4208 4422
Size: 294 902 125 51 2669 167 214
Fragments arranged by size:
2669 902 294 214 167 125 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 468 588 4422
Size: 468 120 3834
Fragments arranged by size:
3834 468 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 463 1201 1349 1918 1984 2062 2395
Size: 385 78 738 148 569 66 78 333
Cuts at: 2395 2848 3033 3102 4039 4422
Size: 453 185 69 937 383
Fragments arranged by size:
937 738 569 453 385 383 333 185
148 78 78 69 66
Bpu10I CC'TnA_GC
Cuts at: 0 73 1231 1427 1585 3396 3797 3917 4422
Size: 73 1158 196 158 1811 401 120 505
Fragments arranged by size:
1811 1158 505 401 196 158 120 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3091 3373 4390 4422
Size: 3091 282 1017 32
Fragments arranged by size:
3091 1017 282 32
BsaAI yAC'GTr
Cuts at: 0 525 588 914 996 1419 2495 4422
Size: 525 63 326 82 423 1076 1927
Fragments arranged by size:
1927 1076 525 423 326 82 63
BsaBI GATnn'nnATC
Cuts at: 0 3850 4422
Size: 3850 572
BsaHI Gr'CG_yC
Cuts at: 0 161 260 1706 1958 1973 2273 4106 4332
Size: 161 99 1446 252 15 300 1833 226
Cuts at: 4332 4422
Size: 90
Fragments arranged by size:
1833 1446 300 252 226 161 99 90
15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 358 388 1582 1612 1696 1726
Size: 103 30 225 30 1194 30 84 30
Cuts at: 1726 2071 2101 3739 3769 4168 4198 4422
Size: 345 30 1638 30 399 30 224
Fragments arranged by size:
1638 1194 399 345 225 224 103 84
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1078 1098 1701 1704 1942 1981 2113 3193
Size: 1078 20 603 3 238 39 132 1080
Cuts at: 3193 3627 4036 4116 4422
Size: 434 409 80 306
Fragments arranged by size:
1080 1078 603 434 409 306 238 132
80 39 20 3
BseYI C'CCAG_C
Cuts at: 0 782 1212 1347 1384 1401 1478 1757 2436
Size: 782 430 135 37 17 77 279 679
Cuts at: 2436 3150 3177 4422
Size: 714 27 1245
Fragments arranged by size:
1245 782 714 679 430 279 135 77
37 27 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 391 1578 2095 2773 3061 4074 4422
Size: 262 129 1187 517 678 288 1013 348
Fragments arranged by size:
1187 1013 678 517 348 288 262 129
BsiEI CG_ry'CG
Cuts at: 0 2673 4097 4422
Size: 2673 1424 325
Fragments arranged by size:
2673 1424 325
BsiHKAI G_wGCw'C
Cuts at: 0 23 473 1433 1937 2531 2840 3107 3314
Size: 23 450 960 504 594 309 267 207
Cuts at: 3314 3869 4422
Size: 555 553
Fragments arranged by size:
960 594 555 553 504 450 309 267
207 23
BsmI GAATG_Cn'
Cuts at: 0 183 1923 2229 4422
Size: 183 1740 306 2193
Fragments arranged by size:
2193 1740 306 183
BsmBI CGTCTCn'nnnn_
Cuts at: 0 730 913 1713 4422
Size: 730 183 800 2709
Fragments arranged by size:
2709 800 730 183
Bsp1286I G_dGCh'C
Cuts at: 0 23 89 151 421 449 473 913 1311
Size: 23 66 62 270 28 24 440 398
Cuts at: 1311 1433 1445 1562 1579 1690 1937 2531 2840
Size: 122 12 117 17 111 247 594 309
Cuts at: 2840 3107 3314 3376 3589 3796 3869 3907 3923
Size: 267 207 62 213 207 73 38 16
Cuts at: 3923 3974 4047 4214 4385 4422
Size: 51 73 167 171 37
Fragments arranged by size:
594 440 398 309 270 267 247 213
207 207 171 167 122 117 111 73
73 66 62 62 51 38 37 28
24 23 17 16 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 260 1920 2855 3560 3884 4178 4422
Size: 260 1660 935 705 324 294 244
Fragments arranged by size:
1660 935 705 324 294 260 244
BsrBI CCG'CTC
Cuts at: 0 731 1299 3359 4422
Size: 731 568 2060 1063
Fragments arranged by size:
2060 1063 731 568
BsrDI GCAATG_nn'
Cuts at: 0 2166 4422
Size: 2166 2256
BsrFI r'CCGG_y
Cuts at: 0 316 1470 1795 2242 2779 4115 4422
Size: 316 1154 325 447 537 1336 307
Fragments arranged by size:
1336 1154 537 447 325 316 307
BssSI C'ACGA_G
Cuts at: 0 460 573 4342 4422
Size: 460 113 3769 80
Fragments arranged by size:
3769 460 113 80
BstAPI GCAn_nnn'nTGC
Cuts at: 0 302 438 1488 1770 4422
Size: 302 136 1050 282 2652
Fragments arranged by size:
2652 1050 302 282 136
BstEII G'GTnAC_C
Cuts at: 0 51 511 3069 4410 4422
Size: 51 460 2558 1341 12
Fragments arranged by size:
2558 1341 460 51 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 630 1668 3126 4422
Size: 630 1038 1458 1296
Fragments arranged by size:
1458 1296 1038 630
BstYI r'GATC_y
Cuts at: 0 536 2546 3382 4422
Size: 536 2010 836 1040
Fragments arranged by size:
2010 1040 836 536
Bsu36I CC'TnA_GG
Cuts at: 0 4205 4422
Size: 4205 217
BtgI C'CryG_G
Cuts at: 0 1856 1922 2216 2363 3539 3579 4422
Size: 1856 66 294 147 1176 40 843
Fragments arranged by size:
1856 1176 843 294 147 66 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 227 1641 2286 2922 3144 3594 4422
Size: 227 1414 645 636 222 450 828
Fragments arranged by size:
1414 828 645 636 450 227 222
BtsI GCAGTG_nn'
Cuts at: 0 23 250 895 3288 4422
Size: 23 227 645 2393 1134
Fragments arranged by size:
2393 1134 645 227 23
DraIII CAC_nnn'GTG
Cuts at: 0 302 1329 4422
Size: 302 1027 3093
Fragments arranged by size:
3093 1027 302
DrdI GACnn_nn'nnGTC
Cuts at: 0 462 4422
Size: 462 3960
EaeI y'GGCC_r
Cuts at: 0 59 590 1468 1472 1649 1885 1940 1985
Size: 59 531 878 4 177 236 55 45
Cuts at: 1985 2240 2624 3896 4094 4137 4422
Size: 255 384 1272 198 43 285
Fragments arranged by size:
1272 878 531 384 285 255 236 198
177 59 55 45 43 4
EagI C'GGCC_G
Cuts at: 0 4094 4422
Size: 4094 328
EarI CTCTTCn'nnn_
Cuts at: 0 1438 2212 2713 2731 3211 3247 3953 4422
Size: 1438 774 501 18 480 36 706 469
Fragments arranged by size:
1438 774 706 501 480 469 36 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 803 1291 4422
Size: 803 488 3131
Fragments arranged by size:
3131 803 488
EcoICRI GAG'CTC
Cuts at: 0 1431 4422
Size: 1431 2991
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 463 1201 1349 1918 1984 2062 2395
Size: 385 78 738 148 569 66 78 333
Cuts at: 2395 2848 3033 3102 4039 4422
Size: 453 185 69 937 383
Fragments arranged by size:
937 738 569 453 385 383 333 185
148 78 78 69 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 433 1966 3938 4422
Size: 433 1533 1972 484
Fragments arranged by size:
1972 1533 484 433
EcoO109I rG'GnC_Cy
Cuts at: 0 350 425 495 969 1351 1502 1575 1576
Size: 350 75 70 474 382 151 73 1
Cuts at: 1576 1767 3431 3792 3793 3903 3904 4014 4210
Size: 191 1664 361 1 110 1 110 196
Cuts at: 4210 4221 4381 4422
Size: 11 160 41
Fragments arranged by size:
1664 474 382 361 350 196 191 160
151 110 110 75 73 70 41 11
1 1 1
EcoRI G'AATT_C
Cuts at: 0 3443 4422
Size: 3443 979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2406 3705 4422
Size: 2406 1299 717
Fragments arranged by size:
2406 1299 717
FseI GG_CCGG'CC
Cuts at: 0 1474 4422
Size: 1474 2948
HaeII r_GCGC'y
Cuts at: 0 939 1976 2276 3151 4109 4422
Size: 939 1037 300 875 958 313
Fragments arranged by size:
1037 958 939 875 313 300
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 460 492 922 954 1696 1728 1774 1806
Size: 460 32 430 32 742 32 46 32
Cuts at: 1806 4168 4200 4422
Size: 2362 32 222
Fragments arranged by size:
2362 742 460 430 222 46 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1116 1611 2450 3051 4422
Size: 1116 495 839 601 1371
Fragments arranged by size:
1371 1116 839 601 495
KasI G'GCGC_C
Cuts at: 0 1972 2272 4105 4422
Size: 1972 300 1833 317
Fragments arranged by size:
1972 1833 317 300
KpnI G_GTAC'C
Cuts at: 0 3539 4422
Size: 3539 883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 2501 4061 4422
Size: 971 1530 1560 361
Fragments arranged by size:
1560 1530 971 361
MscI TGG'CCA
Cuts at: 0 2626 4422
Size: 2626 1796
MslI CAynn'nnrTG
Cuts at: 0 169 282 523 1492 2393 2593 4422
Size: 169 113 241 969 901 200 1829
Fragments arranged by size:
1829 969 901 241 200 169 113
MspA1I CmG'CkG
Cuts at: 0 501 782 882 1140 1347 1737 1761 2127
Size: 501 281 100 258 207 390 24 366
Cuts at: 2127 4160 4407 4422
Size: 2033 247 15
Fragments arranged by size:
2033 501 390 366 281 258 247 207
100 24 15
NaeI GCC'GGC
Cuts at: 0 318 1472 1797 2244 2781 4117 4422
Size: 318 1154 325 447 537 1336 305
Fragments arranged by size:
1336 1154 537 447 325 318 305
NarI GG'CG_CC
Cuts at: 0 1973 2273 4106 4422
Size: 1973 300 1833 316
Fragments arranged by size:
1973 1833 316 300
NcoI C'CATG_G
Cuts at: 0 3539 4422
Size: 3539 883
NgoMIV G'CCGG_C
Cuts at: 0 316 1470 1795 2242 2779 4115 4422
Size: 316 1154 325 447 537 1336 307
Fragments arranged by size:
1336 1154 537 447 325 316 307
NruI TCG'CGA
Cuts at: 0 1130 4422
Size: 1130 3292
NspI r_CATG'y
Cuts at: 0 1491 4422
Size: 1491 2931
PasI CC'CwG_GG
Cuts at: 0 421 1604 2266 3661 4422
Size: 421 1183 662 1395 761
Fragments arranged by size:
1395 1183 761 662 421
PflMI CCAn_nnn'nTGG
Cuts at: 0 306 4422
Size: 306 4116
PfoI T'CCnGG_A
Cuts at: 0 1748 1903 1961 2039 4422
Size: 1748 155 58 78 2383
Fragments arranged by size:
2383 1748 155 78 58
PmlI CAC'GTG
Cuts at: 0 914 1419 2495 4422
Size: 914 505 1076 1927
Fragments arranged by size:
1927 1076 914 505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2071 2103 4422
Size: 2071 32 2319
Fragments arranged by size:
2319 2071 32
PpuMI rG'GwC_Cy
Cuts at: 0 350 425 495 969 1351 3431 4422
Size: 350 75 70 474 382 2080 991
Fragments arranged by size:
2080 991 474 382 350 75 70
PshAI GACnn'nnGTC
Cuts at: 0 341 4422
Size: 341 4081
PspOMI G'GGCC_C
Cuts at: 0 1575 3792 3903 3970 4043 4210 4381 4422
Size: 1575 2217 111 67 73 167 171 41
Fragments arranged by size:
2217 1575 171 167 111 73 67 41
PspXI vC'TCGA_Gb
Cuts at: 0 1520 4422
Size: 1520 2902
PstI C_TGCA'G
Cuts at: 0 434 506 1766 2506 2666 3575 4313 4422
Size: 434 72 1260 740 160 909 738 109
Fragments arranged by size:
1260 909 740 738 434 160 109 72
PvuII CAG'CTG
Cuts at: 0 882 1140 1347 1737 1761 2127 4407 4422
Size: 882 258 207 390 24 366 2280 15
Fragments arranged by size:
2280 882 390 366 258 207 24 15
SacI G_AGCT'C
Cuts at: 0 1433 4422
Size: 1433 2989
SalI G'TCGA_C
Cuts at: 0 2448 4422
Size: 2448 1974
SanDI GG'GwC_CC
Cuts at: 0 425 3431 4422
Size: 425 3006 991
Fragments arranged by size:
3006 991 425
SapI GCTCTTCn'nnn_
Cuts at: 0 1438 2731 4422
Size: 1438 1293 1691
Fragments arranged by size:
1691 1438 1293
SbfI CC_TGCA'GG
Cuts at: 0 4313 4422
Size: 4313 109
ScaI AGT'ACT
Cuts at: 0 2893 4422
Size: 2893 1529
SexAI A'CCwGG_T
Cuts at: 0 1613 3053 4322 4422
Size: 1613 1440 1269 100
Fragments arranged by size:
1613 1440 1269 100
SfcI C'TryA_G
Cuts at: 0 430 502 1173 1762 2502 2662 3571 4309
Size: 430 72 671 589 740 160 909 738
Cuts at: 4309 4422
Size: 113
Fragments arranged by size:
909 740 738 671 589 430 160 113
72
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2261 4422
Size: 2261 2161
SfoI GGC'GCC
Cuts at: 0 1974 2274 4107 4422
Size: 1974 300 1833 315
Fragments arranged by size:
1974 1833 315 300
SmaI CCC'GGG
Cuts at: 0 1573 1684 2258 3369 3783 3975 4042 4422
Size: 1573 111 574 1111 414 192 67 380
Fragments arranged by size:
1573 1111 574 414 380 192 111 67
SmlI C'TyrA_G
Cuts at: 0 1520 3106 3388 4369 4422
Size: 1520 1586 282 981 53
Fragments arranged by size:
1586 1520 981 282 53
SpeI A'CTAG_T
Cuts at: 0 2853 4422
Size: 2853 1569
SphI G_CATG'C
Cuts at: 0 1491 4422
Size: 1491 2931
StuI AGG'CCT
Cuts at: 0 71 2980 4367 4422
Size: 71 2909 1387 55
Fragments arranged by size:
2909 1387 71 55
StyI C'CwwG_G
Cuts at: 0 66 1511 1553 2024 2454 3539 3689 3899
Size: 66 1445 42 471 430 1085 150 210
Cuts at: 3899 4422
Size: 523
Fragments arranged by size:
1445 1085 523 471 430 210 150 66
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 313 811 1321 1340 1492 2571 2583
Size: 79 234 498 510 19 152 1079 12
Cuts at: 2583 2683 2893 4422
Size: 100 210 1529
Fragments arranged by size:
1529 1079 510 498 234 210 152 100
79 19 12
TatI w'GTAC_w
Cuts at: 0 2891 3320 4422
Size: 2891 429 1102
Fragments arranged by size:
2891 1102 429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 18 50 103 135 4422
Size: 18 32 53 32 4287
Fragments arranged by size:
4287 53 32 32 18
Tth111I GACn'n_nGTC
Cuts at: 0 382 1993 2350 3483 4422
Size: 382 1611 357 1133 939
Fragments arranged by size:
1611 1133 939 382 357
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 954 1326 1344 2031 2235 3687 4422
Size: 954 372 18 687 204 1452 735
Fragments arranged by size:
1452 954 735 687 372 204 18
XhoI C'TCGA_G
Cuts at: 0 1520 4422
Size: 1520 2902
XmaI C'CCGG_G
Cuts at: 0 1571 1682 2256 3367 3781 3973 4040 4422
Size: 1571 111 574 1111 414 192 67 382
Fragments arranged by size:
1571 1111 574 414 382 192 111 67
XmnI GAAnn'nnTTC
Cuts at: 0 409 2389 4422
Size: 409 1980 2033
Fragments arranged by size:
2033 1980 409
ZraI GAC'GTC
Cuts at: 0 162 1707 1959 4422
Size: 162 1545 252 2463
Fragments arranged by size:
2463 1545 252 162
Enzymes that do cut:
AarI AatII AccI Acc65I AfeI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AcuI AflII AgeI AhdI AscI AseI AsiSI
AvrII BciVI BfrBI BmgBI BmtI BsaI BsaWI BsiWI
BspEI BspHI BsrGI BssHII BstBI BstZ17I ClaI CspCI
DraI EcoRV FspI FspAI HindIII HpaI MfeI MluI
NdeI NheI NotI NsiI PacI PciI PmeI PsiI
PsrI PvuI RsrII SacII SgfI SgrAI SnaBI SrfI
SspI SwaI XbaI