Restriction map for
pFN21ASDA0817
(Linear) (Six-base) MAPSORT of: KIAA0817.seq Check: 5393 from: 1 to: 8334
KIAA0817.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 93 556 978 1122 1665 2419 2426 2728
Size: 93 463 422 144 543 754 7 302
Cuts at: 2728 4072 8334
Size: 1344 4262
Fragments arranged by size:
4262 1344 754 543 463 422 302 144
93 7
AatII G_ACGT'C
Cuts at: 0 5580 8334
Size: 5580 2754
AccI GT'mk_AC
Cuts at: 0 4832 5574 8334
Size: 4832 742 2760
Fragments arranged by size:
4832 2760 742
Acc65I G'GTAC_C
Cuts at: 0 2750 8334
Size: 2750 5584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 912 1309 1327 1836 2638 5155 5468
Size: 351 561 397 18 509 802 2517 313
Cuts at: 5468 5892 8334
Size: 424 2442
Fragments arranged by size:
2517 2442 802 561 509 424 397 351
313 18
AflIII A'CryG_T
Cuts at: 0 917 1545 3611 4859 6286 6961 8334
Size: 917 628 2066 1248 1427 675 1373
Fragments arranged by size:
2066 1427 1373 1248 917 675 628
AhdI GACnn_n'nnGTC
Cuts at: 0 3758 6824 6986 7164 8334
Size: 3758 3066 162 178 1170
Fragments arranged by size:
3758 3066 1170 178 162
AleI CACnn'nnGTG
Cuts at: 0 2858 3610 4342 4774 5188 7255 7330 8334
Size: 2858 752 732 432 414 2067 75 1004
Fragments arranged by size:
2858 2067 1004 752 732 432 414 75
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 370 5784 6079 6192 8334
Size: 370 5414 295 113 2142
Fragments arranged by size:
5414 2142 370 295 113
AlwNI CAG_nnn'CTG
Cuts at: 0 108 1483 1709 1969 2242 2658 3308 3936
Size: 108 1375 226 260 273 416 650 628
Cuts at: 3936 4194 4401 4455 4743 4746 6075 6641 7704
Size: 258 207 54 288 3 1329 566 1063
Cuts at: 7704 7824 8334
Size: 120 510
Fragments arranged by size:
1375 1329 1063 650 628 566 510 416
288 273 260 258 226 207 120 108
54 3
ApaI G_GGCC'C
Cuts at: 0 75 992 1333 2927 3325 4033 4231 4480
Size: 75 917 341 1594 398 708 198 249
Cuts at: 4480 5306 7427 7673 8027 8099 8171 8334
Size: 826 2121 246 354 72 72 163
Fragments arranged by size:
2121 1594 917 826 708 398 354 341
249 246 198 163 75 72 72
ApaLI G'TGCA_C
Cuts at: 0 240 1399 1903 5808 6927 8334
Size: 240 1159 504 3905 1119 1407
Fragments arranged by size:
3905 1407 1159 1119 504 240
ApoI r'AATT_y
Cuts at: 0 7246 8334
Size: 7246 1088
AvaI C'yCGr_G
Cuts at: 0 74 426 984 1715 3252 3278 3557 3748
Size: 74 352 558 731 1537 26 279 191
Cuts at: 3748 4033 4906 5006 7427 8107 8334
Size: 285 873 100 2421 680 227
Fragments arranged by size:
2421 1537 873 731 680 558 352 285
279 227 191 100 74 26
AvrII C'CTAG_G
Cuts at: 0 1924 3213 8334
Size: 1924 1289 5121
Fragments arranged by size:
5121 1924 1289
BamHI G'GATC_C
Cuts at: 0 206 3552 7440 8334
Size: 206 3346 3888 894
Fragments arranged by size:
3888 3346 894 206
BanI G'GyrC_C
Cuts at: 0 117 544 697 963 1632 1653 1736 1885
Size: 117 427 153 266 669 21 83 149
Cuts at: 1885 2393 2478 2611 2702 2750 2804 2900 3050
Size: 508 85 133 91 48 54 96 150
Cuts at: 3050 3514 4534 4768 5480 6194 6353 7474 7675
Size: 464 1020 234 712 714 159 1121 201
Cuts at: 7675 7995 8185 8334
Size: 320 190 149
Fragments arranged by size:
1121 1020 714 712 669 508 464 427
320 266 234 201 190 159 153 150
149 149 133 117 96 91 85 83
54 48 21
BanII G_rGCy'C
Cuts at: 0 75 959 992 1333 2324 2436 2927 3325
Size: 75 884 33 341 991 112 491 398
Cuts at: 3325 3488 3634 4009 4033 4094 4231 4480 4788
Size: 163 146 375 24 61 137 249 308
Cuts at: 4788 4913 5144 5306 5536 6082 6503 7193 7427
Size: 125 231 162 230 546 421 690 234
Cuts at: 7427 7673 7958 7988 8027 8099 8114 8171 8334
Size: 246 285 30 39 72 15 57 163
Fragments arranged by size:
991 884 690 546 491 421 398 375
341 308 285 249 246 234 231 230
163 163 162 146 137 125 112 75
72 61 57 39 33 30 24 15
BbeI G_GCGC'C
Cuts at: 0 121 701 6198 7478 7679 8189 8334
Size: 121 580 5497 1280 201 510 145
Fragments arranged by size:
5497 1280 580 510 201 145 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 1989 5745 6842 7740 7752 8334
Size: 1989 3756 1097 898 12 582
Fragments arranged by size:
3756 1989 1097 898 582 12
BbvCI CC'TCA_GC
Cuts at: 0 3085 5166 8334
Size: 3085 2081 3168
Fragments arranged by size:
3168 3085 2081
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 7594 7628 8334
Size: 7594 34 706
Fragments arranged by size:
7594 706 34
BglI GCCn_nnn'nGGC
Cuts at: 0 431 1846 2597 2743 2998 3110 3386 4398
Size: 431 1415 751 146 255 112 276 1012
Cuts at: 4398 6310 6524 6902 7819 7953 8166 8334
Size: 1912 214 378 917 134 213 168
Fragments arranged by size:
1912 1415 1012 917 751 431 378 276
255 214 213 168 146 134 112
BglII A'GATC_T
Cuts at: 0 1958 2300 8334
Size: 1958 342 6034
Fragments arranged by size:
6034 1958 342
BlpI GC'TnA_GC
Cuts at: 0 522 1484 3811 7686 8334
Size: 522 962 2327 3875 648
Fragments arranged by size:
3875 2327 962 648 522
Bme1580I G_kGCm'C
Cuts at: 0 75 244 427 992 1206 1333 1403 1637
Size: 75 169 183 565 214 127 70 234
Cuts at: 1637 1907 2249 2616 2927 3325 3446 3519 4033
Size: 270 342 367 311 398 121 73 514
Cuts at: 4033 4067 4231 4480 4523 5306 5812 6356 6931
Size: 34 164 249 43 783 506 544 575
Cuts at: 6931 7018 7427 7673 8027 8099 8171 8334
Size: 87 409 246 354 72 72 163
Fragments arranged by size:
783 575 565 544 514 506 409 398
367 354 342 311 270 249 246 234
214 183 169 164 163 127 121 87
75 73 72 72 70 43 34
BmgBI CAC'GTC
Cuts at: 0 598 918 2006 3021 5903 7166 7281 8334
Size: 598 320 1088 1015 2882 1263 115 1053
Fragments arranged by size:
2882 1263 1088 1053 1015 598 320 115
BmrI ACTGGGnnnn_n'
Cuts at: 0 946 960 1523 2902 3637 3969 4178 6822
Size: 946 14 563 1379 735 332 209 2644
Cuts at: 6822 7031 8334
Size: 209 1303
Fragments arranged by size:
2644 1379 1303 946 735 563 332 209
209 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1977 4932 5139 6165 8334
Size: 1977 2955 207 1026 2169
Fragments arranged by size:
2955 2169 1977 1026 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 960 1070 2032 2338 3644 4586 4768 4962
Size: 960 110 962 306 1306 942 182 194
Cuts at: 4962 5134 5651 6726 8137 8334
Size: 172 517 1075 1411 197
Fragments arranged by size:
1411 1306 1075 962 960 942 517 306
197 194 182 172 110
Bpu10I CC'TnA_GC
Cuts at: 0 1931 3085 5166 6164 8334
Size: 1931 1154 2081 998 2170
Fragments arranged by size:
2170 2081 1931 1154 998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7603 8334
Size: 7603 731
BsaI GGTCTCn'nnnn_
Cuts at: 0 4267 4816 5854 7652 8334
Size: 4267 549 1038 1798 682
Fragments arranged by size:
4267 1798 1038 682 549
BsaAI yAC'GTr
Cuts at: 0 245 1281 3149 4860 7530 8334
Size: 245 1036 1868 1711 2670 804
Fragments arranged by size:
2670 1868 1711 1036 804 245
BsaHI Gr'CG_yC
Cuts at: 0 118 698 4571 5577 5644 6195 6294 7358
Size: 118 580 3873 1006 67 551 99 1064
Cuts at: 7358 7475 7676 8186 8334
Size: 117 201 510 148
Fragments arranged by size:
3873 1064 1006 580 551 510 201 148
118 117 99 67
BsaWI w'CCGG_w
Cuts at: 0 203 5582 8334
Size: 203 5379 2752
Fragments arranged by size:
5379 2752 203
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 306 336 769 799 1060 1090
Size: 259 30 17 30 433 30 261 30
Cuts at: 1090 1216 1246 1408 1438 1669 1699 1854 1884
Size: 126 30 162 30 231 30 155 30
Cuts at: 1884 2788 2818 5809 5839 5986 6016 7132 7162
Size: 904 30 2991 30 147 30 1116 30
Cuts at: 7162 8135 8165 8334
Size: 973 30 169
Fragments arranged by size:
2991 1116 973 904 433 261 259 231
169 162 155 147 126 30 30 30
30 30 30 30 30 30 30 30
30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 972 1068 2113 2518 2644 3592 3742 3966
Size: 972 96 1045 405 126 948 150 224
Cuts at: 3966 5329 6814 7463 7792 8334
Size: 1363 1485 649 329 542
Fragments arranged by size:
1485 1363 1045 972 948 649 542 405
329 224 150 126 96
BseYI C'CCAG_C
Cuts at: 0 51 197 390 488 634 1585 2084 2111
Size: 51 146 193 98 146 951 499 27
Cuts at: 2111 2774 2996 4112 4404 4548 5520 7104 7386
Size: 663 222 1116 292 144 972 1584 282
Cuts at: 7386 7435 7479 7647 7951 8080 8092 8257 8334
Size: 49 44 168 304 129 12 165 77
Fragments arranged by size:
1584 1116 972 951 663 499 304 292
282 222 193 168 165 146 146 144
129 98 77 51 49 44 27 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 595 1742 2032 2325 2511 3198 3789
Size: 343 252 1147 290 293 186 687 591
Cuts at: 3789 3940 4306 4906 5457 5697 5902 6429 7258
Size: 151 366 600 551 240 205 527 829
Cuts at: 7258 8212 8334
Size: 954 122
Fragments arranged by size:
1147 954 829 687 600 591 551 527
366 343 293 290 252 240 205 186
151 122
BsiEI CG_ry'CG
Cuts at: 0 1602 7218 8334
Size: 1602 5616 1116
Fragments arranged by size:
5616 1602 1116
BsiHKAI G_wGCw'C
Cuts at: 0 244 316 523 959 1403 1907 2563 3368
Size: 244 72 207 436 444 504 656 805
Cuts at: 3368 3747 4913 5812 6165 6931 7610 7802 8334
Size: 379 1166 899 353 766 679 192 532
Fragments arranged by size:
1166 899 805 766 679 656 532 504
444 436 379 353 244 207 192 72
BsmI GAATG_Cn'
Cuts at: 0 1732 4063 6559 6583 8334
Size: 1732 2331 2496 24 1751
Fragments arranged by size:
2496 2331 1751 1732 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2128 5584 6364 7003 8334
Size: 2128 3456 780 639 1331
Fragments arranged by size:
3456 2128 1331 780 639
Bsp1286I G_dGCh'C
Cuts at: 0 75 244 316 427 523 959 992 1206
Size: 75 169 72 111 96 436 33 214
Cuts at: 1206 1333 1403 1637 1907 2249 2324 2436 2563
Size: 127 70 234 270 342 75 112 127
Cuts at: 2563 2616 2927 3325 3368 3446 3488 3519 3634
Size: 53 311 398 43 78 42 31 115
Cuts at: 3634 3747 4009 4033 4067 4094 4231 4480 4523
Size: 113 262 24 34 27 137 249 43
Cuts at: 4523 4788 4913 5144 5306 5536 5812 6082 6165
Size: 265 125 231 162 230 276 270 83
Cuts at: 6165 6356 6503 6931 7018 7193 7427 7610 7673
Size: 191 147 428 87 175 234 183 63
Cuts at: 7673 7802 7958 7988 8027 8099 8114 8171 8334
Size: 129 156 30 39 72 15 57 163
Fragments arranged by size:
436 428 398 342 311 276 270 270
265 262 249 234 234 231 230 214
191 183 175 169 163 162 156 147
137 129 127 127 125 115 113 112
111 96 87 83 78 75 75 72
72 70 63 57 53 43 43 42
39 34 33 31 30 27 24 15
BspEI T'CCGG_A
Cuts at: 0 5582 8334
Size: 5582 2752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 93 395 556 617 978 994 1122 1665
Size: 93 302 161 61 361 16 128 543
Cuts at: 1665 2419 2426 2728 2839 3076 3208 3239 4072
Size: 754 7 302 111 237 132 31 833
Cuts at: 4072 5596 6098 6700 7646 8334
Size: 1524 502 602 946 688
Fragments arranged by size:
1524 946 833 754 688 602 543 502
361 302 302 237 161 132 128 111
93 61 31 16 7
BsrBI CCG'CTC
Cuts at: 0 2330 2643 4437 4496 5288 7979 8334
Size: 2330 313 1794 59 792 2691 355
Fragments arranged by size:
2691 2330 1794 792 355 313 59
BsrDI GCAATG_nn'
Cuts at: 0 1401 2436 2880 4164 4618 5265 5709 6201
Size: 1401 1035 444 1284 454 647 444 492
Cuts at: 6201 8334
Size: 2133
Fragments arranged by size:
2133 1401 1284 1035 647 492 454 444
444
BsrFI r'CCGG_y
Cuts at: 0 862 1226 1601 2591 3380 3422 3562 4765
Size: 862 364 375 990 789 42 140 1203
Cuts at: 4765 5940 6421 6695 7580 7847 7947 8017 8334
Size: 1175 481 274 885 267 100 70 317
Fragments arranged by size:
1203 1175 990 885 862 789 481 375
364 317 274 267 140 100 70 42
BsrGI T'GTAC_A
Cuts at: 0 1691 2787 4415 7368 8334
Size: 1691 1096 1628 2953 966
Fragments arranged by size:
2953 1691 1628 1096 966
BssSI C'ACGA_G
Cuts at: 0 2854 3949 4837 7603 8230 8334
Size: 2854 1095 888 2766 627 104
Fragments arranged by size:
2854 2766 1095 888 627 104
BstAPI GCAn_nnn'nTGC
Cuts at: 0 108 970 1736 3803 5480 5597 6935 7704
Size: 108 862 766 2067 1677 117 1338 769
Cuts at: 7704 8226 8334
Size: 522 108
Fragments arranged by size:
2067 1677 1338 862 769 766 522 117
108 108
BstEII G'GTnAC_C
Cuts at: 0 763 7896 8334
Size: 763 7133 438
Fragments arranged by size:
7133 763 438
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 634 1365 1806 2227 2386 2860 3124
Size: 30 604 731 441 421 159 474 264
Cuts at: 3124 4248 5026 5943 6946 8161 8334
Size: 1124 778 917 1003 1215 173
Fragments arranged by size:
1215 1124 1003 917 778 731 604 474
441 421 264 173 159 30
BstYI r'GATC_y
Cuts at: 0 206 1958 2210 2300 3552 7440 8334
Size: 206 1752 252 90 1252 3888 894
Fragments arranged by size:
3888 1752 1252 894 252 206 90
Bsu36I CC'TnA_GG
Cuts at: 0 342 1508 4106 6332 8334
Size: 342 1166 2598 2226 2002
Fragments arranged by size:
2598 2226 2002 1166 342
BtgI C'CryG_G
Cuts at: 0 23 444 540 851 1178 1215 1608 1873
Size: 23 421 96 311 327 37 393 265
Cuts at: 1873 2800 2978 3058 3168 3459 4340 4530 4640
Size: 927 178 80 110 291 881 190 110
Cuts at: 4640 5186 5484 6396 6431 6548 7295 7489 7510
Size: 546 298 912 35 117 747 194 21
Cuts at: 7510 7566 8036 8334
Size: 56 470 298
Fragments arranged by size:
927 912 881 747 546 470 421 393
327 311 298 298 291 265 194 190
178 117 110 110 96 80 56 37
35 23 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2831 4472 6114 8334
Size: 2831 1641 1642 2220
Fragments arranged by size:
2831 2220 1642 1641
BtsI GCAGTG_nn'
Cuts at: 0 457 1235 3099 3135 3186 3306 3366 3465
Size: 457 778 1864 36 51 120 60 99
Cuts at: 3465 3519 4125 4304 5598 5812 5959 6076 6507
Size: 54 606 179 1294 214 147 117 431
Cuts at: 6507 6603 6655 7123 7470 7559 8075 8143 8212
Size: 96 52 468 347 89 516 68 69
Cuts at: 8212 8271 8334
Size: 59 63
Fragments arranged by size:
1864 1294 778 606 516 468 457 431
347 214 179 147 120 117 99 96
89 69 68 63 60 59 54 52
51 36
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3462 3497 6694 6729 8334
Size: 3462 35 3197 35 1605
Fragments arranged by size:
3462 3197 1605 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 239 466 651 3460 4367 5480 7795 8334
Size: 239 227 185 2809 907 1113 2315 539
Fragments arranged by size:
2809 2315 1113 907 539 239 227 185
DrdI GACnn_nn'nnGTC
Cuts at: 0 1705 7306 7357 7545 7585 7744 8334
Size: 1705 5601 51 188 40 159 590
Fragments arranged by size:
5601 1705 590 188 159 51 40
EaeI y'GGCC_r
Cuts at: 0 20 44 848 1170 1599 2678 3165 3462
Size: 20 24 804 322 429 1079 487 297
Cuts at: 3462 3719 3900 4527 6107 6240 6948 7110 7215
Size: 257 181 627 1580 133 708 162 105
Cuts at: 7215 7507 7874 8138 8334
Size: 292 367 264 196
Fragments arranged by size:
1580 1079 804 708 627 487 429 367
322 297 292 264 257 196 181 162
133 105 24 20
EagI C'GGCC_G
Cuts at: 0 1599 7215 8334
Size: 1599 5616 1119
Fragments arranged by size:
5616 1599 1119
EarI CTCTTCn'nnn_
Cuts at: 0 370 1078 1093 2176 2614 7714 7786 8334
Size: 370 708 15 1083 438 5100 72 548
Fragments arranged by size:
5100 1083 708 548 438 370 72 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 314 777 1087 1163 2444 3248 7255 7926
Size: 314 463 310 76 1281 804 4007 671
Cuts at: 7926 7963 8334
Size: 37 371
Fragments arranged by size:
4007 1281 804 671 463 371 314 310
76 37
EcoICRI GAG'CTC
Cuts at: 0 957 4911 8334
Size: 957 3954 3423
Fragments arranged by size:
3954 3423 957
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 912 960 1070 1309 1327 1836 2032
Size: 351 561 48 110 239 18 509 196
Cuts at: 2032 2338 2638 3644 4586 4768 4962 5134 5155
Size: 306 300 1006 942 182 194 172 21
Cuts at: 5155 5468 5651 5892 6726 8137 8334
Size: 313 183 241 834 1411 197
Fragments arranged by size:
1411 1006 942 834 561 509 351 313
306 300 241 239 197 196 194 183
182 172 110 48 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 2422 4393 7807 8334
Size: 10 2412 1971 3414 527
Fragments arranged by size:
3414 2412 1971 527 10
EcoO109I rG'GnC_Cy
Cuts at: 0 71 186 496 663 744 810 988 989
Size: 71 115 310 167 81 66 178 1
Cuts at: 989 1329 1719 1772 1879 1984 2239 2462 2535
Size: 340 390 53 107 105 255 223 73
Cuts at: 2535 2923 2924 2964 3321 3322 3450 3752 3917
Size: 388 1 40 357 1 128 302 165
Cuts at: 3917 3933 4029 4030 4227 4228 4380 4476 4590
Size: 16 96 1 197 1 152 96 114
Cuts at: 4590 4629 5302 5303 5468 5489 5708 6136 6302
Size: 39 673 1 165 21 219 428 166
Cuts at: 6302 6689 6826 7210 7424 7515 7701 8024 8032
Size: 387 137 384 214 91 186 323 8
Cuts at: 8032 8168 8334
Size: 136 166
Fragments arranged by size:
673 428 390 388 387 384 357 340
323 310 302 255 223 219 214 197
186 178 167 166 166 165 165 152
137 136 128 115 114 107 105 96
96 91 81 73 71 66 53 40
39 21 16 8 1 1 1 1
1 1
FseI GG_CCGG'CC
Cuts at: 0 1605 8334
Size: 1605 6729
HaeII r_GCGC'y
Cuts at: 0 121 701 1525 2112 2154 5208 6198 7207
Size: 121 580 824 587 42 3054 990 1009
Cuts at: 7207 7478 7679 7848 8189 8334
Size: 271 201 169 341 145
Fragments arranged by size:
3054 1009 990 824 587 580 341 271
201 169 145 121 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 1060 1092 1669 1701 3115 3147
Size: 259 32 769 32 577 32 1414 32
Cuts at: 3147 3744 3776 6810 6842 6972 7004 7150 7182
Size: 597 32 3034 32 130 32 146 32
Cuts at: 7182 8334
Size: 1152
Fragments arranged by size:
3034 1414 1152 769 597 577 259 146
130 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 5253 5575 8334
Size: 5253 322 2759
Fragments arranged by size:
5253 2759 322
HindIII A'AGCT_T
Cuts at: 0 335 5450 8334
Size: 335 5115 2884
Fragments arranged by size:
5115 2884 335
KasI G'GCGC_C
Cuts at: 0 117 697 6194 7474 7675 8185 8334
Size: 117 580 5497 1280 201 510 149
Fragments arranged by size:
5497 1280 580 510 201 149 117
KpnI G_GTAC'C
Cuts at: 0 2754 8334
Size: 2754 5580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3851 5713 6660 8334
Size: 3851 1862 947 1674
Fragments arranged by size:
3851 1862 1674 947
MscI TGG'CCA
Cuts at: 0 22 1172 2680 3167 3464 6950 7876 8140
Size: 22 1150 1508 487 297 3486 926 264
Cuts at: 8140 8334
Size: 194
Fragments arranged by size:
3486 1508 1150 926 487 297 264 194
22
MslI CAynn'nnrTG
Cuts at: 0 842 2198 2858 3610 4342 4774 5188 5941
Size: 842 1356 660 752 732 432 414 753
Cuts at: 5941 6401 7135 7255 7330 8323 8334
Size: 460 734 120 75 993 11
Fragments arranged by size:
1356 993 842 753 752 734 732 660
460 432 414 120 75 11
MspA1I CmG'CkG
Cuts at: 0 99 291 575 694 1115 1598 1802 1943
Size: 99 192 284 119 421 483 204 141
Cuts at: 1943 2996 3011 3060 3108 3272 4016 4263 4525
Size: 1053 15 49 48 164 744 247 262
Cuts at: 4525 4564 4743 4758 5657 6245 6266 6413 6515
Size: 39 179 15 899 588 21 147 102
Cuts at: 6515 6560 6660 6676 7104 7512 7568 7833 8157
Size: 45 100 16 428 408 56 265 324
Cuts at: 8157 8334
Size: 177
Fragments arranged by size:
1053 899 744 588 483 428 421 408
324 284 265 262 247 204 192 179
177 164 147 141 119 102 100 99
56 49 48 45 39 21 16 15
15
NaeI GCC'GGC
Cuts at: 0 864 1603 2593 3382 3424 6697 7849 7949
Size: 864 739 990 789 42 3273 1152 100
Cuts at: 7949 8334
Size: 385
Fragments arranged by size:
3273 1152 990 864 789 739 385 100
42
NarI GG'CG_CC
Cuts at: 0 118 698 6195 7475 7676 8186 8334
Size: 118 580 5497 1280 201 510 148
Fragments arranged by size:
5497 1280 580 510 201 148 118
NcoI C'CATG_G
Cuts at: 0 23 1178 1215 3168 4340 5186 5484 6431
Size: 23 1155 37 1953 1172 846 298 947
Cuts at: 6431 8036 8334
Size: 1605 298
Fragments arranged by size:
1953 1605 1172 1155 947 846 298 298
37 23
NdeI CA'TA_TG
Cuts at: 0 4515 8334
Size: 4515 3819
NgoMIV G'CCGG_C
Cuts at: 0 862 1601 2591 3380 3422 6695 7847 7947
Size: 862 739 990 789 42 3273 1152 100
Cuts at: 7947 8334
Size: 387
Fragments arranged by size:
3273 1152 990 862 789 739 387 100
42
NspI r_CATG'y
Cuts at: 0 1294 1549 2588 5832 6290 6965 7022 8328
Size: 1294 255 1039 3244 458 675 57 1306
Cuts at: 8328 8334
Size: 6
Fragments arranged by size:
3244 1306 1294 1039 675 458 255 57
6
PasI CC'CwG_GG
Cuts at: 0 6 67 561 759 1890 2155 2242 4395
Size: 6 61 494 198 1131 265 87 2153
Cuts at: 4395 5388 6153 6471 8027 8334
Size: 993 765 318 1556 307
Fragments arranged by size:
2153 1556 1131 993 765 494 318 307
265 198 87 61 6
PciI A'CATG_T
Cuts at: 0 1545 6286 6961 8334
Size: 1545 4741 675 1373
Fragments arranged by size:
4741 1545 1373 675
PflMI CCAn_nnn'nTGG
Cuts at: 0 797 1179 3460 4194 4341 4367 4641 5334
Size: 797 382 2281 734 147 26 274 693
Cuts at: 5334 5520 8334
Size: 186 2814
Fragments arranged by size:
2814 2281 797 734 693 382 274 186
147 26
PfoI T'CCnGG_A
Cuts at: 0 227 2315 3848 5054 5111 6742 6914 7443
Size: 227 2088 1533 1206 57 1631 172 529
Cuts at: 7443 8334
Size: 891
Fragments arranged by size:
2088 1631 1533 1206 891 529 227 172
57
PmlI CAC'GTG
Cuts at: 0 1281 3149 8334
Size: 1281 1868 5185
Fragments arranged by size:
5185 1868 1281
PpuMI rG'GwC_Cy
Cuts at: 0 496 663 744 810 1719 1772 1879 2462
Size: 496 167 81 66 909 53 107 583
Cuts at: 2462 2535 3450 3752 4590 5708 6136 6689 6826
Size: 73 915 302 838 1118 428 553 137
Cuts at: 6826 8032 8334
Size: 1206 302
Fragments arranged by size:
1206 1118 915 909 838 583 553 496
428 302 302 167 137 107 81 73
66 53
PshAI GACnn'nnGTC
Cuts at: 0 815 8334
Size: 815 7519
PspOMI G'GGCC_C
Cuts at: 0 71 988 1329 2923 3321 4029 4227 4476
Size: 71 917 341 1594 398 708 198 249
Cuts at: 4476 5302 7423 7669 8023 8095 8167 8334
Size: 826 2121 246 354 72 72 167
Fragments arranged by size:
2121 1594 917 826 708 398 354 341
249 246 198 167 72 72 71
PstI C_TGCA'G
Cuts at: 0 515 2150 2650 2849 3295 4083 5593 6068
Size: 515 1635 500 199 446 788 1510 475
Cuts at: 6068 6902 7416 8334
Size: 834 514 918
Fragments arranged by size:
1635 1510 918 834 788 515 514 500
475 446 199
PvuII CAG'CTG
Cuts at: 0 694 1802 4263 4743 4758 6413 6676 8157
Size: 694 1108 2461 480 15 1655 263 1481
Cuts at: 8157 8334
Size: 177
Fragments arranged by size:
2461 1655 1481 1108 694 480 263 177
15
RsrII CG'GwC_CG
Cuts at: 0 3873 8334
Size: 3873 4461
SacI G_AGCT'C
Cuts at: 0 959 4913 8334
Size: 959 3954 3421
Fragments arranged by size:
3954 3421 959
SacII CC_GC'GG
Cuts at: 0 3061 7513 7569 8334
Size: 3061 4452 56 765
Fragments arranged by size:
4452 3061 765 56
SalI G'TCGA_C
Cuts at: 0 5573 8334
Size: 5573 2761
SanDI GG'GwC_CC
Cuts at: 0 663 1719 1772 2462 3450 3752 8032 8334
Size: 663 1056 53 690 988 302 4280 302
Fragments arranged by size:
4280 1056 988 690 663 302 302 53
SapI GCTCTTCn'nnn_
Cuts at: 0 7714 8334
Size: 7714 620
SbfI CC_TGCA'GG
Cuts at: 0 515 6902 8334
Size: 515 6387 1432
Fragments arranged by size:
6387 1432 515
ScaI AGT'ACT
Cuts at: 0 6784 8236 8334
Size: 6784 1452 98
Fragments arranged by size:
6784 1452 98
SexAI A'CCwGG_T
Cuts at: 0 5246 5476 8334
Size: 5246 230 2858
Fragments arranged by size:
5246 2858 230
SfcI C'TryA_G
Cuts at: 0 150 511 2146 2646 2845 2905 3291 3733
Size: 150 361 1635 500 199 60 386 442
Cuts at: 3733 4079 5589 6064 6898 7412 8334
Size: 346 1510 475 834 514 922
Fragments arranged by size:
1635 1510 922 834 514 500 475 442
386 361 346 199 150 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1846 6310 8334
Size: 1846 4464 2024
Fragments arranged by size:
4464 2024 1846
SfoI GGC'GCC
Cuts at: 0 119 699 6196 7476 7677 8187 8334
Size: 119 580 5497 1280 201 510 147
Fragments arranged by size:
5497 1280 580 510 201 147 119
SmaI CCC'GGG
Cuts at: 0 76 428 1717 3254 3280 3559 3750 4035
Size: 76 352 1289 1537 26 279 191 285
Cuts at: 4035 7429 8334
Size: 3394 905
Fragments arranged by size:
3394 1537 1289 905 352 285 279 191
76 26
SmlI C'TyrA_G
Cuts at: 0 7618 8107 8334
Size: 7618 489 227
Fragments arranged by size:
7618 489 227
SphI G_CATG'C
Cuts at: 0 2588 8334
Size: 2588 5746
SrfI GCCC'GGGC
Cuts at: 0 76 8334
Size: 76 8258
StuI AGG'CCT
Cuts at: 0 998 1840 1929 2017 4688 8334
Size: 998 842 89 88 2671 3646
Fragments arranged by size:
3646 2671 998 842 89 88
StyI C'CwwG_G
Cuts at: 0 23 41 1178 1215 1333 1455 1740 1778
Size: 23 18 1137 37 118 122 285 38
Cuts at: 1778 1924 3168 3213 4340 5186 5484 6431 6629
Size: 146 1244 45 1127 846 298 947 198
Cuts at: 6629 6764 7892 8036 8334
Size: 135 1128 144 298
Fragments arranged by size:
1244 1137 1128 1127 947 846 298 298
285 198 146 144 135 122 118 45
38 37 23 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 626 751 871 1420 1461 1816 2041 2103
Size: 626 125 120 549 41 355 225 62
Cuts at: 2103 2545 2728 2943 4186 4764 6804 8193 8334
Size: 442 183 215 1243 578 2040 1389 141
Fragments arranged by size:
2040 1389 1243 626 578 549 442 355
225 215 183 141 125 120 62 41
TatI w'GTAC_w
Cuts at: 0 1691 2787 4415 6782 7368 8234 8334
Size: 1691 1096 1628 2367 586 866 100
Fragments arranged by size:
2367 1691 1628 1096 866 586 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 223 255 306 338 1216 1248 1408 1440
Size: 223 32 51 32 878 32 160 32
Cuts at: 1440 1854 1886 2788 2820 4980 5012 5577 5609
Size: 414 32 902 32 2160 32 565 32
Cuts at: 5609 5809 5841 6910 6942 7132 7164 8048 8080
Size: 200 32 1069 32 190 32 884 32
Cuts at: 8080 8135 8167 8334
Size: 55 32 167
Fragments arranged by size:
2160 1069 902 884 878 565 414 223
200 190 167 160 55 51 32 32
32 32 32 32 32 32 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 769 8334
Size: 769 7565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4545 6235 7821 8334
Size: 4545 1690 1586 513
Fragments arranged by size:
4545 1690 1586 513
XhoI C'TCGA_G
Cuts at: 0 8107 8334
Size: 8107 227
XmaI C'CCGG_G
Cuts at: 0 74 426 1715 3252 3278 3557 3748 4033
Size: 74 352 1289 1537 26 279 191 285
Cuts at: 4033 7427 8334
Size: 3394 907
Fragments arranged by size:
3394 1537 1289 907 352 285 279 191
74 26
XmnI GAAnn'nnTTC
Cuts at: 0 7088 8334
Size: 7088 1246
ZraI GAC'GTC
Cuts at: 0 5578 8334
Size: 5578 2756
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI
HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI
PstI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AloI AscI AseI AsiSI
BaeI BciVI BclI BfrBI BmtI BsaBI BsiWI BspHI
BssHII BstBI BstZ17I ClaI DraI EcoRI EcoRV FalI
FspI FspAI HpaI MfeI MluI NheI NotI NruI
NsiI PacI PmeI PpiI PsiI PspXI PsrI PvuI
SgfI SgrAI SnaBI SpeI SspI SwaI XbaI