Restriction map for
pFN21ASDA0798
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspI StuI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 1493 GCGG CCGC(-3/-1)
AlwI 267 GGATC GGATC(4/5)
AlwNI 67 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 75 GGGCCC GGGCC^C
BanII 75 GGGCCC G[GA]GC[CT]^C
BbsI 805 GTCTTC GAAGAC(2/6)
BcnI 99 CCGGG CC^[GC]GG
BglII 116 AGATCT A^GATCT
BmyI 75 GGGCCC G[AGT]GC[ACT]^C
BpuAI 805 GTCTTC GAAGAC(2/6)
BsaAI 613 CACGTA [CT]AC^GT[GA]
BsaBI 253 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 651 GTCTC GTCTC(1/5)
Bsp1286I 75 GGGCCC G[AGT]GC[ACT]^C
BspMI 128 ACCTGC ACCTGC(4/8)
BstYI 116 AGATCT [GA]^GATC[CT]
DdeI 653 CTCAG C^T[ACGT]AG
DraII 75 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 196 CCATGG C^C[GA][CT]GG
EcoO109I 75 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 758 GAATTC G^AATTC
EcoT22I 323 ATGCAT ATGCA^T
HaeIII 76 GGCC GG^CC
HapII 99 CCGG C^CGG
HincII 430 GTCAAC GT[CT]^[GA]AC
HindII 430 GTCAAC GT[CT]^[GA]AC
HpaII 99 CCGG C^CGG
MaeII 614 ACGT A^CGT
MamI 253 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 116 AGATCT [GA]^GATC[CT]
MspI 99 CCGG C^CGG
MwoI 890 GCTTCCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 99 CCGGG CC^[GC]GG
NcoI 196 CCATGG C^CATGG
NlaIV 75 GGGCCC GG[ACGT]^[ACGT]CC
NsiI 323 ATGCAT ATGCA^T
PleI 1407 GAGTC GAGTC(4/5)
TfiI 227 GAATC G^A[AT]TC
XhoII 116 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BbvI 66 GCAGC GCAGC(8/12)
526 GCAGC
BsgI 1458 GTGCAG GTGCAG(16/14)
1626 GTGCAG
Cfr13I 75 GGGCC G^G[ACGT]CC
76 GGCCC
EcoT14I 196 CCATGG C^C[AT][AT]GG
1736 CCAAGG
Fnu4HI 66 GCAGC GC^[ACGT]GC
526 GCAGC
HinfI 227 GAATC G^A[ACGT]TC
1407 GAGTC
ItaI 66 GCAGC GC^[ACGT]GC
526 GCAGC
MslI 1115 CATATATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1283 CATATCTATG
PstI 262 CTGCAG CTGCA^G
524 CTGCAG
Sau96I 75 GGGCC G^G[ACGT]CC
76 GGCCC
SphI 989 GCATGC GCATG^C
1325 GCATGC
StyI 196 CCATGG C^C[AT][AT]GG
1736 CCAAGG
TaqI 225 TCGA T^CGA
1166 TCGA
TseI 66 GCAGC G^C[AT]GC
526 GCAGC
TthHB8I 225 TCGA T^CGA
1166 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BclI BpmI BsaJI BseRI Bsp1407I
BspHI BsrDI BsrGI BsrI BssKI
BstF5I BstNI Cac8I DpnII EarI
EcoRII FbaI FokI HphI Ksp632I
MaeIII MboI MboII MnlI MseI
MvaI NdeI NdeII NlaIII NspI
RcaI RsaI SapI Sau3AI ScrFI
SfaNI SfcI SspBI Tru9I Tsp509I
TspRI XmnI