(Circular) (Six-base) MAPSORT of: pFN21ASDA0798.seq Check: 8956 from: 1 to: 6685 pFN21ASDA0798 6685 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4495 278 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4028 1937 Size: 2091 4594 Acc65I G'GTAC_C Cuts at: 4006 4006 Size: 6685 AclI AA'CG_TT Cuts at: 1563 4812 5185 1563 Size: 3249 373 3063 Fragments arranged by size: 3249 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 4748 6132 6400 1632 Size: 704 2412 1384 268 1917 Fragments arranged by size: 2412 1917 1384 704 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6488 AflIII A'CryG_T Cuts at: 1203 5584 6561 1203 Size: 4381 977 1327 Fragments arranged by size: 4381 1327 977 AgeI A'CCGG_T Cuts at: 5516 5516 Size: 6685 AhdI GACnn_n'nnGTC Cuts at: 5414 5414 Size: 6685 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6685 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6685 AlwNI CAG_nnn'CTG Cuts at: 2095 4428 6000 2095 Size: 2333 1572 2780 Fragments arranged by size: 2780 2333 1572 ApaI G_GGCC'C Cuts at: 2102 2102 Size: 6685 ApaLI G'TGCA_C Cuts at: 4742 5898 4742 Size: 1156 5529 ApoI r'AATT_y Cuts at: 1493 2440 2492 2555 2781 3705 3994 4220 4372 Size: 947 52 63 226 924 289 226 152 Cuts at: 4372 6481 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 947 924 289 226 226 152 63 52 AseI AT'TA_AT Cuts at: 160 1032 5239 160 Size: 872 4207 1606 Fragments arranged by size: 4207 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 AvaI C'yCGr_G Cuts at: 1870 1943 4010 1870 Size: 73 2067 4545 Fragments arranged by size: 4545 2067 73 BamHI G'GATC_C Cuts at: 4015 4382 4015 Size: 367 6318 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4006 5461 618 Size: 325 217 627 2219 1455 1842 Fragments arranged by size: 2219 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2102 4004 1391 Size: 711 1902 4072 Fragments arranged by size: 4072 1902 711 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2821 928 Size: 971 922 4792 Fragments arranged by size: 4792 971 922 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4876 4910 1903 Size: 34 2939 34 3678 Fragments arranged by size: 3678 2939 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4577 5787 4577 Size: 1210 5475 BclI T'GATC_A Cuts at: 1548 2025 2278 2686 1548 Size: 477 253 408 5547 Fragments arranged by size: 5547 477 408 253 BfrBI ATG'CAT Cuts at: 2348 2348 Size: 6685 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5296 136 Size: 107 122 71 631 605 3624 1525 Fragments arranged by size: 3624 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2139 6680 2139 Size: 4541 2144 BlpI GC'TnA_GC Cuts at: 4102 4102 Size: 6685 Bme1580I G_kGCm'C Cuts at: 1790 2102 4746 5902 1790 Size: 312 2644 1156 2573 Fragments arranged by size: 2644 2573 1156 312 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6685 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3012 4416 5374 Size: 416 53 83 399 24 1595 1404 958 Cuts at: 5374 442 Size: 1753 Fragments arranged by size: 1753 1595 1404 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6685 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2734 2895 2979 3483 3567 3651 3819 Size: 833 662 161 84 504 84 84 168 Cuts at: 3819 5345 1239 Size: 1526 2579 Fragments arranged by size: 2579 1526 833 662 504 168 161 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4810 5675 5973 4810 Size: 865 298 5522 Fragments arranged by size: 5522 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5348 882 Size: 617 3849 2219 Fragments arranged by size: 3849 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2638 3982 4476 493 Size: 711 1434 1344 494 2702 Fragments arranged by size: 2702 1434 1344 711 494 BsaBI GATnn'nnATC Cuts at: 2280 6679 2280 Size: 4399 2286 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4492 4874 5509 275 Size: 53 83 186 936 2959 382 635 1451 Fragments arranged by size: 2959 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5118 5516 5790 5937 6348 5118 Size: 398 274 147 411 5455 Fragments arranged by size: 5455 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6460 Fragments arranged by size: 6460 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2223 3916 1186 Size: 910 127 1693 3955 Fragments arranged by size: 3955 1693 910 127 BseYI C'CCAG_C Cuts at: 5888 6384 5888 Size: 496 6189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3502 3670 1163 Size: 573 1766 168 4178 Fragments arranged by size: 4178 1766 573 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4135 4896 5924 1540 Size: 275 186 2134 761 1028 2301 Fragments arranged by size: 2301 2134 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4746 4831 5902 4746 Size: 85 1071 5529 Fragments arranged by size: 5529 1071 85 BsmI GAATG_Cn' Cuts at: 4196 4289 4196 Size: 93 6592 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 4004 4746 4831 5902 1391 Size: 399 312 1902 742 85 1071 2174 Fragments arranged by size: 2174 1902 1071 742 399 312 85 BspEI T'CCGG_A Cuts at: 6348 6348 Size: 6685 BspHI T'CATG_A Cuts at: 2401 2617 2689 4574 6304 2401 Size: 216 72 1885 1730 2782 Fragments arranged by size: 2782 1885 1730 216 72 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2160 4040 844 Size: 1316 1880 3489 Fragments arranged by size: 3489 1880 1316 BsrBI CCG'CTC Cuts at: 1394 1823 4572 1394 Size: 429 2749 3507 Fragments arranged by size: 3507 2749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2902 3154 3574 5180 5354 65 Size: 1183 1654 252 420 1606 174 1396 Fragments arranged by size: 1654 1606 1396 1183 420 252 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5329 5516 1700 Size: 110 3519 187 2869 Fragments arranged by size: 3519 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2938 3274 3442 3610 3694 3946 96 Size: 2842 336 168 168 84 252 2835 Fragments arranged by size: 2842 2835 336 252 168 168 84 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6685 BssSI C'ACGA_G Cuts at: 4745 5757 4745 Size: 1012 5673 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6685 BstBI TT'CG_AA Cuts at: 2007 5489 2007 Size: 3482 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6685 BstYI r'GATC_y Cuts at: 1972 2139 4015 4382 4767 4784 6236 6322 6334 Size: 167 1876 367 385 17 1452 86 12 Cuts at: 6334 6680 1972 Size: 346 1977 Fragments arranged by size: 1977 1876 1452 385 367 346 167 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2219 513 Size: 552 1154 4979 Fragments arranged by size: 4979 1154 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5946 Fragments arranged by size: 5946 683 56 BtsI GCAGTG_nn' Cuts at: 2650 2818 3238 3406 3490 3658 3910 4201 4995 Size: 168 420 168 84 168 252 291 794 Cuts at: 4995 5015 2650 Size: 20 4320 Fragments arranged by size: 4320 794 420 291 252 168 168 168 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6685 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2884 2919 3136 3171 3556 3591 529 Size: 35 2320 35 217 35 385 35 3623 Fragments arranged by size: 3623 2320 385 217 35 35 35 35 DraI TTT'AAA Cuts at: 3989 3989 Size: 6685 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5692 809 Size: 507 4376 1802 Fragments arranged by size: 4376 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4132 5021 6650 Size: 54 1425 50 275 18 2302 889 1629 Cuts at: 6650 8 Size: 43 Fragments arranged by size: 2302 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4132 1537 Size: 275 2320 4090 Fragments arranged by size: 4090 2320 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2312 2834 3002 3170 3254 3422 3711 3742 Size: 959 522 168 168 84 168 289 31 Cuts at: 3742 3842 4615 1353 Size: 100 773 3423 Fragments arranged by size: 3423 959 773 522 289 168 168 168 100 84 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5272 5646 5792 387 Size: 1064 138 3683 374 146 1280 Fragments arranged by size: 3683 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2336 2734 2895 2979 3483 3567 Size: 393 440 264 398 161 84 504 84 Cuts at: 3567 3651 3819 4748 5345 6132 6400 1239 Size: 84 168 929 597 787 268 1524 Fragments arranged by size: 1524 929 787 597 504 440 398 393 268 264 168 161 84 84 84 EcoO109I rG'GnC_Cy Cuts at: 2099 2099 Size: 6685 EcoRI G'AATT_C Cuts at: 2781 3994 4372 2781 Size: 1213 378 5094 Fragments arranged by size: 5094 1213 378 FspI TGC'GCA Cuts at: 5191 5191 Size: 6685 HaeII r_GCGC'y Cuts at: 1678 5832 1678 Size: 4154 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 2495 2527 2842 Size: 32 135 32 605 32 286 32 315 Cuts at: 2842 2874 3178 3210 3430 3462 3514 3546 5326 Size: 32 304 32 220 32 52 32 1780 Cuts at: 5326 5358 5400 5432 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1780 605 315 304 286 220 135 52 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2455 4029 4275 669 Size: 988 281 517 1574 246 3079 Fragments arranged by size: 3079 1574 988 517 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6685 HpaI GTT'AAC Cuts at: 4275 4275 Size: 6685 KpnI G_GTAC'C Cuts at: 4010 4010 Size: 6685 MfeI C'AATT_G Cuts at: 4284 4284 Size: 6685 MluI A'CGCG_T Cuts at: 6561 6561 Size: 6685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2115 2181 5799 5983 1305 Size: 810 66 3618 184 2007 Fragments arranged by size: 3618 2007 810 184 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4863 Fragments arranged by size: 4863 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3142 3310 4643 5002 5161 518 Size: 648 1976 168 1333 359 159 2042 Fragments arranged by size: 2042 1976 1333 648 359 168 159 MspA1I CmG'CkG Cuts at: 1606 4101 4778 5926 6171 1606 Size: 2495 677 1148 245 2120 Fragments arranged by size: 2495 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2219 513 Size: 552 1154 4979 Fragments arranged by size: 4979 1154 552 NdeI CA'TA_TG Cuts at: 387 2891 3227 3563 387 Size: 2504 336 336 3509 Fragments arranged by size: 3509 2504 336 336 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6685 NotI GC'GGCC_GC Cuts at: 4132 4132 Size: 6685 NsiI A_TGCA'T Cuts at: 2350 2350 Size: 6685 NspI r_CATG'y Cuts at: 1132 1229 3016 3352 3399 4043 5588 1132 Size: 97 1787 336 47 644 1545 2229 Fragments arranged by size: 2229 1787 1545 644 336 97 47 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6685 PciI A'CATG_T Cuts at: 5584 5584 Size: 6685 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6685 PmeI GTTT'AAAC Cuts at: 3989 3989 Size: 6685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4754 4786 6267 6299 4754 Size: 32 1481 32 5140 Fragments arranged by size: 5140 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6685 PsiI TTA'TAA Cuts at: 4255 4255 Size: 6685 PspOMI G'GGCC_C Cuts at: 2098 2098 Size: 6685 PstI C_TGCA'G Cuts at: 830 2289 2551 4037 830 Size: 1459 262 1486 3478 Fragments arranged by size: 3478 1486 1459 262 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6685 SalI G'TCGA_C Cuts at: 1936 4027 1936 Size: 2091 4594 SapI GCTCTTCn'nnn_ Cuts at: 2834 3002 3170 3842 2834 Size: 168 168 672 5677 Fragments arranged by size: 5677 672 168 168 SbfI CC_TGCA'GG Cuts at: 4037 4037 Size: 6685 ScaI AGT'ACT Cuts at: 4933 6645 4933 Size: 1712 4973 SfcI C'TryA_G Cuts at: 826 1045 2143 2285 2547 3061 4033 5168 5849 Size: 219 1098 142 262 514 972 1135 681 Cuts at: 5849 6040 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1098 972 681 514 262 219 191 142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6685 SmaI CCC'GGG Cuts at: 1872 4012 1872 Size: 2140 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4789 5690 5952 820 Size: 197 926 2846 901 262 1553 Fragments arranged by size: 2846 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3982 4476 493 Size: 3489 494 2702 Fragments arranged by size: 3489 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6685 SphI G_CATG'C Cuts at: 1132 3016 3352 4043 1132 Size: 1884 336 691 3774 Fragments arranged by size: 3774 1884 691 336 SspI AAT'ATT Cuts at: 5 52 4609 5 Size: 47 4557 2081 Fragments arranged by size: 4557 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6685 StyI C'CwwG_G Cuts at: 513 1065 2219 3759 4124 513 Size: 552 1154 1540 365 3074 Fragments arranged by size: 3074 1540 1154 552 365 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4712 4729 4882 5067 5512 325 Size: 1255 3132 17 153 185 445 1498 Fragments arranged by size: 3132 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2567 2726 2938 Size: 274 80 33 51 1443 590 159 212 Cuts at: 2938 3274 3442 3610 3694 3946 4931 6643 96 Size: 336 168 168 84 252 985 1712 138 Fragments arranged by size: 1712 1443 985 590 336 274 252 212 168 168 159 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6645 6677 6645 Size: 32 6653 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6685 XbaI T'CTAG_A Cuts at: 4021 4021 Size: 6685 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6685 XmaI C'CCGG_G Cuts at: 1870 4010 1870 Size: 2140 4545 XmnI GAAnn'nnTTC Cuts at: 3080 3164 3416 3500 4814 3080 Size: 84 252 84 1314 4951 Fragments arranged by size: 4951 1314 252 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4493 276 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI