(Circular) (Six-base) MAPSORT of: pFN21ASDA0794.seq Check: 5082 from: 1 to: 6196 pFN21ASDA0794 6196 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4006 278 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3539 1937 Size: 1602 4594 Acc65I G'GTAC_C Cuts at: 3517 3517 Size: 6196 AclI AA'CG_TT Cuts at: 1563 3373 4323 4696 1563 Size: 1810 950 373 3063 Fragments arranged by size: 3063 1810 950 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2081 2211 4259 5643 5911 1632 Size: 449 130 2048 1384 268 1917 Fragments arranged by size: 2048 1917 1384 449 268 130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5999 AflIII A'CryG_T Cuts at: 1203 5095 6072 1203 Size: 3892 977 1327 Fragments arranged by size: 3892 1327 977 AgeI A'CCGG_T Cuts at: 2093 5027 2093 Size: 2934 3262 AhdI GACnn_n'nnGTC Cuts at: 4925 4925 Size: 6196 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2581 1184 Size: 1397 4799 AlwNI CAG_nnn'CTG Cuts at: 3939 5511 3939 Size: 1572 4624 ApaLI G'TGCA_C Cuts at: 4253 5409 4253 Size: 1156 5040 ApoI r'AATT_y Cuts at: 1493 3505 3731 3883 5992 1493 Size: 2012 226 152 2109 1697 Fragments arranged by size: 2109 2012 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4750 160 Size: 872 3718 1606 Fragments arranged by size: 3718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 AvaI C'yCGr_G Cuts at: 1870 1943 3521 1870 Size: 73 1578 4545 Fragments arranged by size: 4545 1578 73 BamHI G'GATC_C Cuts at: 3526 3893 3526 Size: 367 5829 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2748 3517 4972 618 Size: 325 217 627 961 769 1455 1842 Fragments arranged by size: 1842 1455 961 769 627 325 217 BanII G_rGCy'C Cuts at: 1391 2042 2199 3515 1391 Size: 651 157 1316 4072 Fragments arranged by size: 4072 1316 651 157 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2886 928 Size: 971 987 4238 Fragments arranged by size: 4238 987 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4387 4421 1903 Size: 34 2450 34 3678 Fragments arranged by size: 3678 2450 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3288 3353 4088 5298 3288 Size: 65 735 1210 4186 Fragments arranged by size: 4186 1210 735 65 BclI T'GATC_A Cuts at: 1548 1548 Size: 6196 BfrBI ATG'CAT Cuts at: 2470 2470 Size: 6196 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4807 136 Size: 107 122 71 631 605 3135 1525 Fragments arranged by size: 3135 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2440 6191 2440 Size: 3751 2445 BlpI GC'TnA_GC Cuts at: 3613 3613 Size: 6196 Bme1580I G_kGCm'C Cuts at: 1790 2260 2851 4257 5413 1790 Size: 470 591 1406 1156 2573 Fragments arranged by size: 2573 1406 1156 591 470 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6196 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2193 3927 4885 Size: 416 53 83 399 24 776 1734 958 Cuts at: 4885 442 Size: 1753 Fragments arranged by size: 1753 1734 958 776 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3332 1056 Size: 2276 3920 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3218 4856 1239 Size: 1979 1638 2579 Fragments arranged by size: 2579 1979 1638 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3444 4321 5186 5484 3444 Size: 877 865 298 4156 Fragments arranged by size: 4156 877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4859 882 Size: 617 3360 2219 Fragments arranged by size: 3360 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3346 3493 3987 493 Size: 711 2142 147 494 2702 Fragments arranged by size: 2702 2142 711 494 147 BsaBI GATnn'nnATC Cuts at: 6190 6190 Size: 6196 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 4003 4385 5020 Size: 53 83 186 936 515 1955 382 635 Cuts at: 5020 275 Size: 1451 Fragments arranged by size: 1955 1451 936 635 515 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2093 4629 5027 5301 5448 5859 2093 Size: 2536 398 274 147 411 2430 Fragments arranged by size: 2536 2430 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 1178 Size: 30 165 30 621 30 5320 Fragments arranged by size: 5320 621 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2421 3017 3116 1186 Size: 1235 596 99 4266 Fragments arranged by size: 4266 1235 596 99 BseYI C'CCAG_C Cuts at: 5399 5895 5399 Size: 496 5700 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2316 3368 1163 Size: 573 580 1052 3991 Fragments arranged by size: 3991 1052 580 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3646 4407 5435 1540 Size: 275 186 1645 761 1028 2301 Fragments arranged by size: 2301 1645 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2314 4257 4342 5413 2314 Size: 1943 85 1071 3097 Fragments arranged by size: 3097 1943 1071 85 BsmI GAATG_Cn' Cuts at: 3707 3800 3707 Size: 93 6103 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2199 2260 2314 2851 3515 4257 Size: 399 252 157 61 54 537 664 742 Cuts at: 4257 4342 5413 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 664 537 399 252 157 85 61 54 BspEI T'CCGG_A Cuts at: 5859 5859 Size: 6196 BspHI T'CATG_A Cuts at: 2644 4085 5815 2644 Size: 1441 1730 3025 Fragments arranged by size: 3025 1730 1441 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2626 3551 844 Size: 1782 925 3489 Fragments arranged by size: 3489 1782 925 BsrBI CCG'CTC Cuts at: 1394 1823 2961 4083 1394 Size: 429 1138 1122 3507 Fragments arranged by size: 3507 1138 1122 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3434 4691 4865 65 Size: 1183 2186 1257 174 1396 Fragments arranged by size: 2186 1396 1257 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 4840 5027 1700 Size: 110 283 2747 187 2869 Fragments arranged by size: 2869 2747 283 187 110 BsrGI T'GTAC_A Cuts at: 96 3475 96 Size: 3379 2817 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6196 BssSI C'ACGA_G Cuts at: 4256 5268 4256 Size: 1012 5184 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6196 BstBI TT'CG_AA Cuts at: 2007 5000 2007 Size: 2993 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6196 BstYI r'GATC_y Cuts at: 1972 2440 3526 3893 4278 4295 5747 5833 5845 Size: 468 1086 367 385 17 1452 86 12 Cuts at: 5845 6191 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1086 468 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2032 2043 2720 513 Size: 552 957 10 11 677 3989 Fragments arranged by size: 3989 957 677 552 11 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2592 487 Size: 683 56 1366 4091 Fragments arranged by size: 4091 1366 683 56 BtsI GCAGTG_nn' Cuts at: 3004 3712 4506 4526 3004 Size: 708 794 20 4674 Fragments arranged by size: 4674 794 708 20 ClaI AT'CG_AT Cuts at: 1554 2415 1554 Size: 861 5335 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2482 2501 2517 2536 529 Size: 35 1918 19 16 19 4189 Fragments arranged by size: 4189 1918 35 19 19 16 DraI TTT'AAA Cuts at: 3500 3500 Size: 6196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5203 809 Size: 507 3887 1802 Fragments arranged by size: 3887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2450 2503 3643 Size: 54 1425 50 275 18 620 53 1140 Cuts at: 3643 4532 6161 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1140 889 620 275 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3643 1537 Size: 275 1831 4090 Fragments arranged by size: 4090 1831 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2187 3033 3312 4126 1353 Size: 834 846 279 814 3423 Fragments arranged by size: 3423 846 834 814 279 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4783 5157 5303 387 Size: 1064 138 3194 374 146 1280 Fragments arranged by size: 3194 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2211 3218 4259 4856 5643 5911 Size: 393 449 130 1007 1041 597 787 268 Cuts at: 5911 1239 Size: 1524 Fragments arranged by size: 1524 1041 1007 787 597 449 393 268 130 EcoRI G'AATT_C Cuts at: 3505 3883 3505 Size: 378 5818 FspI TGC'GCA Cuts at: 4702 4702 Size: 6196 HaeII r_GCGC'y Cuts at: 1678 2060 5343 1678 Size: 382 3283 2531 Fragments arranged by size: 3283 2531 382 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2397 2429 2945 2957 2977 Size: 32 135 32 825 32 516 12 20 Cuts at: 2977 2989 2996 3028 4837 4869 4911 4943 1373 Size: 12 7 32 1809 32 42 32 2626 Fragments arranged by size: 2626 1809 825 516 135 42 32 32 32 32 32 32 20 12 12 7 HincII GTy'rAC Cuts at: 669 1657 1938 3540 3786 669 Size: 988 281 1602 246 3079 Fragments arranged by size: 3079 1602 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6196 HpaI GTT'AAC Cuts at: 3786 3786 Size: 6196 KpnI G_GTAC'C Cuts at: 3521 3521 Size: 6196 MfeI C'AATT_G Cuts at: 3795 3795 Size: 6196 MluI A'CGCG_T Cuts at: 6072 6072 Size: 6196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5310 5494 1305 Size: 4005 184 2007 Fragments arranged by size: 4005 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2505 10 Size: 54 1425 343 673 3701 Fragments arranged by size: 3701 1425 673 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2563 2649 4154 4513 4672 518 Size: 648 1397 86 1505 359 159 2042 Fragments arranged by size: 2042 1505 1397 648 359 159 86 MspA1I CmG'CkG Cuts at: 1606 2045 3118 3179 3265 3612 4289 5437 5682 Size: 439 1073 61 86 347 677 1148 245 Cuts at: 5682 1606 Size: 2120 Fragments arranged by size: 2120 1148 1073 677 439 347 245 86 61 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4687 Fragments arranged by size: 4687 957 552 NdeI CA'TA_TG Cuts at: 387 2121 387 Size: 1734 4462 NheI G'CTAG_C Cuts at: 1052 3328 1052 Size: 2276 3920 NotI GC'GGCC_GC Cuts at: 3643 3643 Size: 6196 NsiI A_TGCA'T Cuts at: 2472 2472 Size: 6196 NspI r_CATG'y Cuts at: 1132 1229 2562 3554 5099 1132 Size: 97 1333 992 1545 2229 Fragments arranged by size: 2229 1545 1333 992 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6196 PciI A'CATG_T Cuts at: 5095 5095 Size: 6196 PflMI CCAn_nnn'nTGG Cuts at: 1108 2306 1108 Size: 1198 4998 PfoI T'CCnGG_A Cuts at: 2355 2613 3145 2355 Size: 258 532 5406 Fragments arranged by size: 5406 532 258 PmeI GTTT'AAAC Cuts at: 3500 3500 Size: 6196 PmlI CAC'GTG Cuts at: 3346 3346 Size: 6196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4265 4297 5778 5810 4265 Size: 32 1481 32 4651 Fragments arranged by size: 4651 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6196 PsiI TTA'TAA Cuts at: 2682 3766 2682 Size: 1084 5112 PstI C_TGCA'G Cuts at: 830 3548 830 Size: 2718 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6196 PvuII CAG'CTG Cuts at: 3179 3265 3179 Size: 86 6110 SacII CC_GC'GG Cuts at: 2046 2046 Size: 6196 SalI G'TCGA_C Cuts at: 1936 3538 1936 Size: 1602 4594 SapI GCTCTTCn'nnn_ Cuts at: 2187 2187 Size: 6196 SbfI CC_TGCA'GG Cuts at: 3548 3548 Size: 6196 ScaI AGT'ACT Cuts at: 4444 6156 4444 Size: 1712 4484 SfcI C'TryA_G Cuts at: 826 1045 3544 4679 5360 5551 826 Size: 219 2499 1135 681 191 1471 Fragments arranged by size: 2499 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6196 SmaI CCC'GGG Cuts at: 1872 3523 1872 Size: 1651 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3459 4300 5201 5463 820 Size: 197 926 1516 841 901 262 1553 Fragments arranged by size: 1553 1516 926 901 841 262 197 SnaBI TAC'GTA Cuts at: 493 3493 3987 493 Size: 3000 494 2702 Fragments arranged by size: 3000 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6196 SphI G_CATG'C Cuts at: 1132 3554 1132 Size: 2422 3774 SspI AAT'ATT Cuts at: 5 52 2608 4120 5 Size: 47 2556 1512 2081 Fragments arranged by size: 2556 2081 1512 47 StuI AGG'CCT Cuts at: 1517 3450 1517 Size: 1933 4263 StyI C'CwwG_G Cuts at: 513 1065 2022 3166 3635 513 Size: 552 957 1144 469 3074 Fragments arranged by size: 3074 1144 957 552 469 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2470 2786 4223 4240 4393 4578 5023 Size: 1255 890 316 1437 17 153 185 445 Cuts at: 5023 325 Size: 1498 Fragments arranged by size: 1498 1437 1255 890 445 316 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3475 4442 6154 Size: 274 80 33 51 1443 1498 967 1712 Cuts at: 6154 96 Size: 138 Fragments arranged by size: 1712 1498 1443 967 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2024 2056 3379 3411 6156 6188 2024 Size: 32 1323 32 2745 32 2032 Fragments arranged by size: 2745 2032 1323 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6196 XbaI T'CTAG_A Cuts at: 3532 3532 Size: 6196 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6196 XmaI C'CCGG_G Cuts at: 1870 3521 1870 Size: 1651 4545 XmnI GAAnn'nnTTC Cuts at: 2250 2376 4325 2250 Size: 126 1949 4121 Fragments arranged by size: 4121 1949 126 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4004 276 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI