(Circular) (Six-base) MAPSORT of: pFN21ASDA0792.seq Check: 3251 from: 1 to: 7150 pFN21ASDA0792 7150 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2881 4175 2881 Size: 1294 5856 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2196 4960 278 Size: 53 83 186 936 660 2764 2468 Fragments arranged by size: 2764 2468 936 660 186 83 53 AccI GT'mk_AC Cuts at: 1937 3471 3540 3981 4493 1937 Size: 1534 69 441 512 4594 Fragments arranged by size: 4594 1534 512 441 69 Acc65I G'GTAC_C Cuts at: 4471 4471 Size: 7150 AclI AA'CG_TT Cuts at: 1563 5277 5650 1563 Size: 3714 373 3063 Fragments arranged by size: 3714 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3131 5213 6597 6865 1632 Size: 1499 2082 1384 268 1917 Fragments arranged by size: 2082 1917 1499 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6953 AflIII A'CryG_T Cuts at: 1203 2772 2783 6049 7026 1203 Size: 1569 11 3266 977 1327 Fragments arranged by size: 3266 1569 1327 977 11 AgeI A'CCGG_T Cuts at: 5981 5981 Size: 7150 AhdI GACnn_n'nnGTC Cuts at: 2290 5879 2290 Size: 3589 3561 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3752 1184 Size: 2568 4582 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2021 2053 3030 3062 2021 Size: 32 977 32 6109 Fragments arranged by size: 6109 977 32 32 AlwNI CAG_nnn'CTG Cuts at: 2407 3061 3289 4893 6465 2407 Size: 654 228 1604 1572 3092 Fragments arranged by size: 3092 1604 1572 654 228 ApaLI G'TGCA_C Cuts at: 5207 6363 5207 Size: 1156 5994 ApoI r'AATT_y Cuts at: 1493 4459 4685 4837 6946 1493 Size: 2966 226 152 2109 1697 Fragments arranged by size: 2966 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5704 160 Size: 872 4672 1606 Fragments arranged by size: 4672 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7150 AvaI C'yCGr_G Cuts at: 1870 1943 2090 4475 1870 Size: 73 147 2385 4545 Fragments arranged by size: 4545 2385 147 73 BamHI G'GATC_C Cuts at: 4008 4480 4847 4008 Size: 472 367 6311 Fragments arranged by size: 6311 472 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2658 3680 4355 4471 5926 Size: 325 217 627 871 1022 675 116 1455 Cuts at: 5926 618 Size: 1842 Fragments arranged by size: 1842 1455 1022 871 675 627 325 217 116 BanII G_rGCy'C Cuts at: 1391 3165 3192 4469 1391 Size: 1774 27 1277 4072 Fragments arranged by size: 4072 1774 1277 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2861 2903 2982 3655 3865 928 Size: 971 962 42 79 673 210 4213 Fragments arranged by size: 4213 971 962 673 210 79 42 BbvCI CC'TCA_GC Cuts at: 4190 4190 Size: 7150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5341 5375 1903 Size: 34 3404 34 3678 Fragments arranged by size: 3678 3404 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2787 5042 6252 2787 Size: 2255 1210 3685 Fragments arranged by size: 3685 2255 1210 BclI T'GATC_A Cuts at: 1548 3777 1548 Size: 2229 4921 BfrBI ATG'CAT Cuts at: 3857 3857 Size: 7150 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3791 5761 136 Size: 107 122 71 631 605 2119 1970 1525 Fragments arranged by size: 2119 1970 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7145 7145 Size: 7150 BlpI GC'TnA_GC Cuts at: 4567 4567 Size: 7150 Bme1580I G_kGCm'C Cuts at: 1790 5211 6367 1790 Size: 3421 1156 2573 Fragments arranged by size: 3421 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3046 1318 Size: 1728 5422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4881 5839 442 Size: 416 53 83 399 24 3464 958 1753 Fragments arranged by size: 3464 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2132 3038 3509 4019 5810 1239 Size: 824 69 906 471 510 1791 2579 Fragments arranged by size: 2579 1791 906 824 510 471 69 Bpu10I CC'TnA_GC Cuts at: 4190 4190 Size: 7150 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3929 5275 6140 6438 3929 Size: 1346 865 298 4641 Fragments arranged by size: 4641 1346 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3590 3758 5813 882 Size: 617 2091 168 2055 2219 Fragments arranged by size: 2219 2091 2055 617 168 BsaAI yAC'GTr Cuts at: 493 1204 2784 4279 4447 4941 493 Size: 711 1580 1495 168 494 2702 Fragments arranged by size: 2702 1580 1495 711 494 168 BsaBI GATnn'nnATC Cuts at: 2311 7144 2311 Size: 4833 2317 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2193 2427 2979 4957 Size: 53 83 186 936 660 234 552 1978 Cuts at: 4957 5339 5974 275 Size: 382 635 1451 Fragments arranged by size: 1978 1451 936 660 635 552 382 234 186 83 53 BsaWI w'CCGG_w Cuts at: 3609 5583 5981 6255 6402 6813 3609 Size: 1974 398 274 147 411 3946 Fragments arranged by size: 3946 1974 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6925 Fragments arranged by size: 6925 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2314 2702 1186 Size: 1128 388 5634 Fragments arranged by size: 5634 1128 388 BseYI C'CCAG_C Cuts at: 2083 2353 2959 3165 3289 3988 6353 6849 2083 Size: 270 606 206 124 699 2365 496 2384 Fragments arranged by size: 2384 2365 699 606 496 270 206 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2591 2822 1163 Size: 573 855 231 5491 Fragments arranged by size: 5491 855 573 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4600 5361 6389 1540 Size: 275 186 2599 761 1028 2301 Fragments arranged by size: 2599 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 3192 5211 5296 6367 2148 Size: 1044 2019 85 1071 2931 Fragments arranged by size: 2931 2019 1071 1044 85 BsmI GAATG_Cn' Cuts at: 4661 4754 4661 Size: 93 7057 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 3165 3192 4469 5211 5296 6367 Size: 399 358 1017 27 1277 742 85 1071 Cuts at: 6367 1391 Size: 2174 Fragments arranged by size: 2174 1277 1071 1017 742 399 358 85 27 BspEI T'CCGG_A Cuts at: 6813 6813 Size: 7150 BspHI T'CATG_A Cuts at: 3525 5039 6769 3525 Size: 1514 1730 3906 Fragments arranged by size: 3906 1730 1514 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2881 2895 4175 4505 844 Size: 2037 14 1280 330 3489 Fragments arranged by size: 3489 2037 1280 330 14 BsrBI CCG'CTC Cuts at: 1394 1823 5037 1394 Size: 429 3214 3507 Fragments arranged by size: 3507 3214 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2262 3730 5645 5819 65 Size: 1183 1014 1468 1915 174 1396 Fragments arranged by size: 1915 1468 1396 1183 1014 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2097 3960 5794 5981 1700 Size: 110 287 1863 1834 187 2869 Fragments arranged by size: 2869 1863 1834 287 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7150 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7150 BssSI C'ACGA_G Cuts at: 5210 6222 5210 Size: 1012 6138 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7150 BstBI TT'CG_AA Cuts at: 2007 5954 2007 Size: 3947 3203 BstEII G'GTnAC_C Cuts at: 1184 2155 1184 Size: 971 6179 BstXI CCAn_nnnn'nTGG Cuts at: 3652 3652 Size: 7150 BstYI r'GATC_y Cuts at: 1972 2403 4008 4480 4847 5232 5249 6701 6787 Size: 431 1605 472 367 385 17 1452 86 Cuts at: 6787 6799 7145 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1605 1452 472 431 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2326 3231 2326 Size: 905 6245 BtgI C'CryG_G Cuts at: 513 1065 3096 3357 4410 513 Size: 552 2031 261 1053 3253 Fragments arranged by size: 3253 2031 1053 552 261 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3688 3898 487 Size: 683 56 2462 210 3739 Fragments arranged by size: 3739 2462 683 210 56 BtsI GCAGTG_nn' Cuts at: 2070 3482 4423 4666 5460 5480 2070 Size: 1412 941 243 794 20 3740 Fragments arranged by size: 3740 1412 941 794 243 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7150 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2914 2949 529 Size: 35 2350 35 4730 Fragments arranged by size: 4730 2350 35 35 DraI TTT'AAA Cuts at: 4454 4454 Size: 7150 DraIII CAC_nnn'GTG Cuts at: 3955 3955 Size: 7150 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4321 6157 809 Size: 507 3005 1836 1802 Fragments arranged by size: 3005 1836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2257 2820 Size: 54 1425 50 275 18 297 130 563 Cuts at: 2820 2935 3099 3783 4597 5486 7115 8 Size: 115 164 684 814 889 1629 43 Fragments arranged by size: 1629 1425 889 814 684 563 297 275 164 130 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4597 1537 Size: 275 2785 4090 Fragments arranged by size: 4090 2785 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2639 2858 3314 4064 4209 5080 1353 Size: 1286 219 456 750 145 871 3423 Fragments arranged by size: 3423 1286 871 750 456 219 145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2719 5737 6111 6257 387 Size: 1064 138 1130 3018 374 146 1280 Fragments arranged by size: 3018 1280 1130 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3190 3190 Size: 7150 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2132 3038 3131 3509 4019 5213 Size: 393 431 69 906 93 378 510 1194 Cuts at: 5213 5810 6597 6865 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1194 906 787 597 510 431 393 378 268 93 69 EcoNI CCTnn'n_nnAGG Cuts at: 2449 2840 3646 4309 2449 Size: 391 806 663 5290 Fragments arranged by size: 5290 806 663 391 EcoO109I rG'GnC_Cy Cuts at: 3265 4102 3265 Size: 837 6313 EcoRI G'AATT_C Cuts at: 4459 4837 4459 Size: 378 6772 FspI TGC'GCA Cuts at: 5656 5656 Size: 7150 HaeII r_GCGC'y Cuts at: 1678 4410 6297 1678 Size: 2732 1887 2531 Fragments arranged by size: 2732 2531 1887 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 2969 3001 5791 Size: 32 135 32 704 32 661 32 2790 Cuts at: 5791 5823 5865 5897 1373 Size: 32 42 32 2626 Fragments arranged by size: 2790 2626 704 661 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2333 2515 4494 4740 669 Size: 988 281 395 182 1979 246 3079 Fragments arranged by size: 3079 1979 988 395 281 246 182 HindIII A'AGCT_T Cuts at: 748 748 Size: 7150 HpaI GTT'AAC Cuts at: 4740 4740 Size: 7150 KpnI G_GTAC'C Cuts at: 4475 4475 Size: 7150 MfeI C'AATT_G Cuts at: 4749 4749 Size: 7150 MluI A'CGCG_T Cuts at: 2772 7026 2772 Size: 4254 2896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3633 6264 6448 1305 Size: 2328 2631 184 2007 Fragments arranged by size: 2631 2328 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2822 2937 3101 3785 10 Size: 54 1425 343 990 115 164 684 3375 Fragments arranged by size: 3375 1425 990 684 343 164 115 54 MslI CAynn'nnrTG Cuts at: 518 1166 2999 3779 5108 5467 5626 518 Size: 648 1833 780 1329 359 159 2042 Fragments arranged by size: 2042 1833 1329 780 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 2199 2806 2908 3273 4022 4374 4566 Size: 477 116 607 102 365 749 352 192 Cuts at: 4566 5243 6391 6636 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 749 677 607 477 365 352 245 192 116 102 NcoI C'CATG_G Cuts at: 513 1065 3096 3357 513 Size: 552 2031 261 4306 Fragments arranged by size: 4306 2031 552 261 NdeI CA'TA_TG Cuts at: 387 387 Size: 7150 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7150 NotI GC'GGCC_GC Cuts at: 4597 4597 Size: 7150 NsiI A_TGCA'T Cuts at: 3859 3859 Size: 7150 NspI r_CATG'y Cuts at: 1132 1229 4258 4508 6053 1132 Size: 97 3029 250 1545 2229 Fragments arranged by size: 3029 2229 1545 250 97 PasI CC'CwG_GG Cuts at: 1348 3061 3580 1348 Size: 1713 519 4918 Fragments arranged by size: 4918 1713 519 PciI A'CATG_T Cuts at: 6049 6049 Size: 7150 PflMI CCAn_nnn'nTGG Cuts at: 1108 2407 3061 4036 1108 Size: 1299 654 975 4222 Fragments arranged by size: 4222 1299 975 654 PfoI T'CCnGG_A Cuts at: 2040 2406 2766 3048 3084 2040 Size: 366 360 282 36 6106 Fragments arranged by size: 6106 366 360 282 36 PmeI GTTT'AAAC Cuts at: 4454 4454 Size: 7150 PmlI CAC'GTG Cuts at: 2784 2784 Size: 7150 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5219 5251 6732 6764 5219 Size: 32 1481 32 5605 Fragments arranged by size: 5605 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3536 1613 Size: 1923 5227 PsiI TTA'TAA Cuts at: 2234 4720 2234 Size: 2486 4664 PstI C_TGCA'G Cuts at: 830 2892 4502 830 Size: 2062 1610 3478 Fragments arranged by size: 3478 2062 1610 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7150 PvuII CAG'CTG Cuts at: 2083 2199 2806 2083 Size: 116 607 6427 Fragments arranged by size: 6427 607 116 SacI G_AGCT'C Cuts at: 3192 3192 Size: 7150 SalI G'TCGA_C Cuts at: 1936 4492 1936 Size: 2556 4594 SapI GCTCTTCn'nnn_ Cuts at: 2858 4209 2858 Size: 1351 5799 SbfI CC_TGCA'GG Cuts at: 2892 4502 2892 Size: 1610 5540 ScaI AGT'ACT Cuts at: 5398 7110 5398 Size: 1712 5438 SexAI A'CCwGG_T Cuts at: 2694 2694 Size: 7150 SfcI C'TryA_G Cuts at: 826 1045 2579 2888 3281 3892 4498 5633 6314 Size: 219 1534 309 393 611 606 1135 681 Cuts at: 6314 6505 826 Size: 191 1471 Fragments arranged by size: 1534 1471 1135 681 611 606 393 309 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7150 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7150 SmaI CCC'GGG Cuts at: 1872 4477 1872 Size: 2605 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3944 5254 6155 6417 820 Size: 197 926 2001 1310 901 262 1553 Fragments arranged by size: 2001 1553 1310 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4447 4941 493 Size: 3954 494 2702 Fragments arranged by size: 3954 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7150 SphI G_CATG'C Cuts at: 1132 4508 1132 Size: 3376 3774 SspI AAT'ATT Cuts at: 5 52 5074 5 Size: 47 5022 2081 Fragments arranged by size: 5022 2081 47 StuI AGG'CCT Cuts at: 1517 3641 3827 4034 4442 1517 Size: 2124 186 207 408 4225 Fragments arranged by size: 4225 2124 408 207 186 StyI C'CwwG_G Cuts at: 513 1065 2058 3096 3357 4035 4589 513 Size: 552 993 1038 261 678 554 3074 Fragments arranged by size: 3074 1038 993 678 554 552 261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5177 5194 5347 5532 5977 325 Size: 1255 3597 17 153 185 445 1498 Fragments arranged by size: 3597 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2679 5396 7108 Size: 274 80 33 51 1443 702 2717 1712 Cuts at: 7108 96 Size: 138 Fragments arranged by size: 2717 1712 1443 702 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2426 2458 2654 2686 3853 3885 7110 7142 2426 Size: 32 196 32 1167 32 3225 32 2434 Fragments arranged by size: 3225 2434 1167 196 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3505 1316 Size: 2189 4961 XbaI T'CTAG_A Cuts at: 3595 4486 3595 Size: 891 6259 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2788 3839 4417 2788 Size: 1051 578 5521 Fragments arranged by size: 5521 1051 578 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7150 XmaI C'CCGG_G Cuts at: 1870 4475 1870 Size: 2605 4545 XmnI GAAnn'nnTTC Cuts at: 3119 5279 3119 Size: 2160 4990 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2194 4958 276 Size: 53 83 186 936 660 2764 2468 Fragments arranged by size: 2764 2468 936 660 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI