Restriction map for
pFN21ASDA0789
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AocI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BcgI BciVI BclI BfrI
BglII BlnI BsaI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoRI EcoRV EcoT14I FbaI FseI
HincII HindII HpaI KpnI KspI
MfeI MluI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccI 643 GTCTAC GT^[AC][GT]AC
AccII 915 CGCG CG^CG
AccIII 1568 TCCGGA T^CCGGA
AcsI 19 AAATTC [GA]^AATT[CT]
Alw44I 1432 GTGCAC G^TGCAC
AlwI 552 GATCC GGATC(4/5)
AlwNI 1049 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1556 AGCGCT AGC^GCT
ApaI 422 GGGCCC GGGCC^C
ApaLI 1432 GTGCAC G^TGCAC
ApoI 19 AAATTC [GA]^AATT[CT]
AspHI 1432 GTGCAC G[AT]GC[AT]^C
AviII 1320 TGCGCA TGC^GCA
BbrPI 1062 CACGTG CAC^GTG
BbvCI 175 GCTGAGG CCTCAGC(-5/-2)
BfaI 944 CTAG C^TAG
BlpI 184 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 184 GCTGAGC GC^T[ACGT]AGC
BsaAI 1062 CACGTG [CT]AC^GT[GA]
BsaBI 459 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1568 TCCGGA [AT]^CCGG[AT]
BseAI 1568 TCCGGA T^CCGGA
BsiHKAI 1432 GTGCAC G[AT]GC[AT]^C
BsmAI 133 GTCTC GTCTC(1/5)
BspEI 1568 TCCGGA T^CCGGA
BspLU11I 764 ACATGT A^CATGT
BstEII 499 GGTTACC G^GT[ACGT]ACC
BstPI 499 GGTTACC G^GT[ACGT]ACC
BstUI 915 CGCG CG^CG
BstXI 1536 CCAGAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 551 AGATCC [GA]^GATC[CT]
CelII 184 GCTGAGC GC^T[ACGT]AGC
DraI 1147 TTTAAA TTT^AAA
DrdI 1456 GACCTCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1375 CCGTGG C^C[GA][CT]GG
EarI 373 GAAGAG CTCTTC(1/4)
Eco47III 1556 AGCGCT AGC^GCT
EcoNI 1446 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 499 GGTTACC G^GT[ACGT]ACC
EcoT22I 208 ATGCAT ATGCA^T
FspI 1320 TGCGCA TGC^GCA
HindIII 307 AAGCTT A^AGCTT
Ksp632I 373 GAAGAG CTCTTC(1/4)
MaeI 944 CTAG C^TAG
MamI 459 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 551 AGATCC [GA]^GATC[CT]
MroI 1568 TCCGGA T^CCGGA
MseI 1148 TTAA T^TAA
MvnI 915 CGCG CG^CG
NsiI 208 ATGCAT ATGCA^T
PaeR7I 1576 CTCGAG C^TCGAG
PmaCI 1062 CACGTG CAC^GTG
PmlI 1062 CACGTG CAC^GTG
PshAI 966 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 373 GAAGAGC GCTCTTC(1/4)
SphI 1323 GCATGC GCATG^C
StuI 1226 AGGCCT AGG^CCT
TfiI 863 GATTC G^A[AT]TC
Tru9I 1148 TTAA T^TAA
XcmI 1385 CCACTCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1576 CTCGAG C^TCGAG
XhoII 551 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 764 ACATGT A^C[GA][CT]GT
1061 ACACGT
AvaI 135 CTCGGG C^[CT]CG[GA]G
1576 CTCGAG
BbeI 526 GGCGCC GGCGC^C
625 GGCGCC
BbsI 1608 GAAGAC GAAGAC(2/6)
97 GTCTTC
BpmI 330 CTGGAG CTGGAG(16/14)
517 CTGGAG
BpuAI 1608 GAAGAC GAAGAC(2/6)
97 GTCTTC
BseRI 431 GAGGAG GAGGAG(10/8)
919 GAGGAG
BsoBI 135 CTCGGG C^[CT]CG[GA]G
1576 CTCGAG
BspMI 260 GCAGGT ACCTGC(4/8)
677 GCAGGT
BsrBI 868 CCGCTC CCGCTC(-3/-3)
1156 GAGCGG
BsrDI 252 CATTGC GCAATG(2/0)
1029 CATTGC
BsrFI 647 ACCGGC [GA]^CCGG[CT]
1514 ACCGGC
Cfr10I 647 ACCGGC [GA]^CCGG[CT]
1514 ACCGGC
DpnII 552 GATC ^GATC
1221 GATC
HgaI 1197 GACGC GACGC(5/10)
1234 GACGC
KasI 526 GGCGCC G^GCGCC
625 GGCGCC
MaeII 566 ACGT A^CGT
1063 ACGT
MboI 552 GATC ^GATC
1221 GATC
MslI 765 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1626 CATCAAGATG
NarI 526 GGCGCC GG^CGCC
625 GGCGCC
NdeII 552 GATC ^GATC
1221 GATC
PflMI 5 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1048 CCAGGTGCTGG
PpuMI 1442 AGGACCT [GA]G^G[AT]CC[CT]
1454 AGGACCT
PstI 161 CTGCAG CTGCA^G
652 CTGCAG
Sau3AI 552 GATC ^GATC
1221 GATC
SfcI 161 CTGCAG C^T[GA][CT]AG
652 CTGCAG
SfoI 526 GGCGCC GGC^GCC
625 GGCGCC
Tsp45I 203 GTGAC ^GT[GC]AC
745 GTGAC
Tsp509I 20 AATT ^AATT
1085 AATT
Van91I 5 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1048 CCAGGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BsaHI BsaJI BsgI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeIII MboII MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV NspI PleI PvuII
RsaI Sau96I ScrFI SfaNI SmlI
TaqI TseI TspRI TthHB8I