Restriction map for
pFN21ASDA0789
(Circular) (Six-base) MAPSORT of: pFN21ASDA0789.seq Check: 2955 from: 1 to: 6424
pFN21ASDA0789 6424 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4234 278
Size: 53 83 186 936 2698 2468
Fragments arranged by size:
2698 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2667 3767 1937
Size: 730 1100 4594
Fragments arranged by size:
4594 1100 730
Acc65I G'GTAC_C
Cuts at: 3745 3745
Size: 6424
AclI AA'CG_TT
Cuts at: 1563 4551 4924 1563
Size: 2988 373 3063
Fragments arranged by size:
3063 2988 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3491 4487 5871 6139 1632
Size: 1859 996 1384 268 1917
Fragments arranged by size:
1917 1859 1384 996 268
AfeI AGC'GCT
Cuts at: 3581 3581
Size: 6424
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6227
AflIII A'CryG_T
Cuts at: 1203 2787 3084 5323 6300 1203
Size: 1584 297 2239 977 1327
Fragments arranged by size:
2239 1584 1327 977 297
AgeI A'CCGG_T
Cuts at: 5255 5255
Size: 6424
AhdI GACnn_n'nnGTC
Cuts at: 5153 5153
Size: 6424
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2937 3069 3461 1184
Size: 1753 132 392 4147
Fragments arranged by size:
4147 1753 392 132
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 6392
AlwNI CAG_nnn'CTG
Cuts at: 3077 4167 5739 3077
Size: 1090 1572 3762
Fragments arranged by size:
3762 1572 1090
ApaI G_GGCC'C
Cuts at: 2449 2449
Size: 6424
ApaLI G'TGCA_C
Cuts at: 3455 4481 5637 3455
Size: 1026 1156 4242
Fragments arranged by size:
4242 1156 1026
ApoI r'AATT_y
Cuts at: 1493 2042 3733 3959 4111 6220 1493
Size: 549 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 549 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4978 160
Size: 872 3946 1606
Fragments arranged by size:
3946 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
AvaI C'yCGr_G
Cuts at: 1870 1943 2158 3599 3749 1870
Size: 73 215 1441 150 4545
Fragments arranged by size:
4545 1441 215 150 73
BamHI G'GATC_C
Cuts at: 3754 4121 3754
Size: 367 6057
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2549 2648 2852 3745 5200
Size: 325 217 627 762 99 204 893 1455
Cuts at: 5200 618
Size: 1842
Fragments arranged by size:
1842 1455 893 762 627 325 217 204
99
BanII G_rGCy'C
Cuts at: 1391 2365 2449 3094 3591 3743 1391
Size: 974 84 645 497 152 4072
Fragments arranged by size:
4072 974 645 497 152 84
BbeI G_GCGC'C
Cuts at: 2553 2652 2553
Size: 99 6325
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2113 3638 928
Size: 971 214 1525 3714
Fragments arranged by size:
3714 1525 971 214
BbvCI CC'TCA_GC
Cuts at: 2199 2199
Size: 6424
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4615 4649 1903
Size: 34 2678 34 3678
Fragments arranged by size:
3678 2678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4316 5526 4316
Size: 1210 5214
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6424
BfrBI ATG'CAT
Cuts at: 2233 2233
Size: 6424
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3299 3333 3516
Size: 107 122 71 631 605 1627 34 183
Cuts at: 3516 5035 136
Size: 1519 1525
Fragments arranged by size:
1627 1525 1519 631 605 183 122 107
71 34
BglII A'GATC_T
Cuts at: 6419 6419
Size: 6424
BlpI GC'TnA_GC
Cuts at: 2208 3841 2208
Size: 1633 4791
Bme1580I G_kGCm'C
Cuts at: 1790 2449 2926 3459 4485 5641 1790
Size: 659 477 533 1026 1156 2573
Fragments arranged by size:
2573 1156 1026 659 533 477
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6424
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4155 5113 442
Size: 416 53 83 399 24 2738 958 1753
Fragments arranged by size:
2738 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2374 2561 5084 1239
Size: 1135 187 2523 2579
Fragments arranged by size:
2579 2523 1135 187
Bpu10I CC'TnA_GC
Cuts at: 2199 3583 2199
Size: 1384 5040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2254 2351 3413 4549 5414 5712 2254
Size: 97 1062 1136 865 298 2966
Fragments arranged by size:
2966 1136 1062 865 298 97
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5087 882
Size: 617 3588 2219
Fragments arranged by size:
3588 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3087 3721 4215 493
Size: 711 1883 634 494 2702
Fragments arranged by size:
2702 1883 711 634 494
BsaBI GATnn'nnATC
Cuts at: 2486 6418 2486
Size: 3932 2492
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2550 2649 3258 4231
Size: 53 83 186 936 1017 99 609 973
Cuts at: 4231 4613 5248 275
Size: 382 635 1451
Fragments arranged by size:
1451 1017 973 936 635 609 382 186
99 83 53
BsaWI w'CCGG_w
Cuts at: 3591 4857 5255 5529 5676 6087 3591
Size: 1266 398 274 147 411 3928
Fragments arranged by size:
3928 1266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6199
Fragments arranged by size:
6199 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2469 2957 1186
Size: 1283 488 4653
Fragments arranged by size:
4653 1283 488
BseYI C'CCAG_C
Cuts at: 2108 2194 3493 3514 5627 6123 2108
Size: 86 1299 21 2113 496 2409
Fragments arranged by size:
2409 2113 1299 496 86 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2733 2800 2953 1163
Size: 573 997 67 153 4634
Fragments arranged by size:
4634 997 573 153 67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3874 4635 5663 1540
Size: 275 186 1873 761 1028 2301
Fragments arranged by size:
2301 1873 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3459 4485 4570 5641 3459
Size: 1026 85 1071 4242
Fragments arranged by size:
4242 1071 1026 85
BsmI GAATG_Cn'
Cuts at: 3935 4028 3935
Size: 93 6331
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2365 2449 2926 3094 3459 3591 3743
Size: 399 575 84 477 168 365 132 152
Cuts at: 3743 4485 4570 5641 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 575 477 399 365 168
152 132 85 84
BspEI T'CCGG_A
Cuts at: 3591 6087 3591
Size: 2496 3928
BspHI T'CATG_A
Cuts at: 4313 6043 4313
Size: 1730 4694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 2691 3779 844
Size: 1430 417 1088 3489
Fragments arranged by size:
3489 1430 1088 417
BsrBI CCG'CTC
Cuts at: 1394 1823 2893 3181 4311 1394
Size: 429 1070 288 1130 3507
Fragments arranged by size:
3507 1130 1070 429 288
BsrDI GCAATG_nn'
Cuts at: 65 1248 2274 3051 4919 5093 65
Size: 1183 1026 777 1868 174 1396
Fragments arranged by size:
1868 1396 1183 1026 777 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2670 3537 5068 5255 1700
Size: 110 860 867 1531 187 2869
Fragments arranged by size:
2869 1531 867 860 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6424
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6424
BssSI C'ACGA_G
Cuts at: 4484 5496 4484
Size: 1012 5412
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2640 2846 2859 794
Size: 1846 206 13 4359
Fragments arranged by size:
4359 1846 206 13
BstBI TT'CG_AA
Cuts at: 2007 5228 2007
Size: 3221 3203
BstEII G'GTnAC_C
Cuts at: 1184 2522 1184
Size: 1338 5086
BstXI CCAn_nnnn'nTGG
Cuts at: 3566 3566
Size: 6424
BstYI r'GATC_y
Cuts at: 1972 2574 3754 4121 4506 4523 5975 6061 6073
Size: 602 1180 367 385 17 1452 86 12
Cuts at: 6073 6419 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1180 602 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3398 513
Size: 552 957 1376 3539
Fragments arranged by size:
3539 1376 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5685
Fragments arranged by size:
5685 683 56
BtsI GCAGTG_nn'
Cuts at: 2721 3940 4734 4754 2721
Size: 1219 794 20 4391
Fragments arranged by size:
4391 1219 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6389
DraI TTT'AAA
Cuts at: 3172 3728 3172
Size: 556 5868
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3485 5431 809
Size: 507 2169 1946 1802
Fragments arranged by size:
2169 1946 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2566 3060
Size: 54 1425 50 275 18 195 541 494
Cuts at: 3060 3111 3232 3325 3371 3871 4760 6389 8
Size: 51 121 93 46 500 889 1629 43
Fragments arranged by size:
1629 1425 889 541 500 494 275 195
121 93 54 51 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3871 1537
Size: 275 2059 4090
Fragments arranged by size:
4090 2059 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2391 4354 1353
Size: 1038 1963 3423
Fragments arranged by size:
3423 1963 1038
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2048 3694 5011 5385 5531 387
Size: 1064 138 459 1646 1317 374 146 1280
Fragments arranged by size:
1646 1317 1280 1064 459 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2374 2561 3491 4487 5084 5871 6139
Size: 393 742 187 930 996 597 787 268
Cuts at: 6139 1239
Size: 1524
Fragments arranged by size:
1524 996 930 787 742 597 393 268
187
EcoNI CCTnn'n_nnAGG
Cuts at: 3473 3473
Size: 6424
EcoO109I rG'GnC_Cy
Cuts at: 2446 3466 3478 2446
Size: 1020 12 5392
Fragments arranged by size:
5392 1020 12
EcoRI G'AATT_C
Cuts at: 3733 4111 3733
Size: 378 6046
FspI TGC'GCA
Cuts at: 3345 4930 3345
Size: 1585 4839
HaeII r_GCGC'y
Cuts at: 1678 2553 2652 3583 5571 1678
Size: 875 99 931 1988 2531
Fragments arranged by size:
2531 1988 931 875 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2877 2909 3192 3224 5065
Size: 32 135 32 1305 32 283 32 1841
Cuts at: 5065 5097 5139 5171 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1841 1305 283 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3768 4014 669
Size: 988 281 1830 246 3079
Fragments arranged by size:
3079 1830 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2330 748
Size: 1582 4842
HpaI GTT'AAC
Cuts at: 4014 4014
Size: 6424
KasI G'GCGC_C
Cuts at: 2549 2648 2549
Size: 99 6325
KpnI G_GTAC'C
Cuts at: 3749 3749
Size: 6424
MfeI C'AATT_G
Cuts at: 4023 4023
Size: 6424
MluI A'CGCG_T
Cuts at: 6300 6300
Size: 6424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5538 5722 1305
Size: 4233 184 2007
Fragments arranged by size:
4233 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 3062 3113 3373 10
Size: 54 1425 343 195 1035 51 260 3061
Fragments arranged by size:
3061 1425 1035 343 260 195 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2792 3653 4382 4741 4900 518
Size: 648 1626 861 729 359 159 2042
Fragments arranged by size:
2042 1626 861 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2153 2180 2198 2680 2774 2836 3198
Size: 444 103 27 18 482 94 62 362
Cuts at: 3198 3493 3840 4517 5665 5910 1606
Size: 295 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 482 444 362 347 295
245 103 94 62 27 18
NarI GG'CG_CC
Cuts at: 2550 2649 2550
Size: 99 6325
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4915
Fragments arranged by size:
4915 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6424
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6424
NotI GC'GGCC_GC
Cuts at: 3871 3871
Size: 6424
NsiI A_TGCA'T
Cuts at: 2235 2235
Size: 6424
NspI r_CATG'y
Cuts at: 1132 1229 2233 2791 3350 3628 3782 5327 1132
Size: 97 1004 558 559 278 154 1545 2229
Fragments arranged by size:
2229 1545 1004 559 558 278 154 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6424
PciI A'CATG_T
Cuts at: 2787 5323 2787
Size: 2536 3888
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2034 3077 1108
Size: 926 1043 4455
Fragments arranged by size:
4455 1043 926
PfoI T'CCnGG_A
Cuts at: 2250 2382 2250
Size: 132 6292
PmeI GTTT'AAAC
Cuts at: 3728 3728
Size: 6424
PmlI CAC'GTG
Cuts at: 3087 3087
Size: 6424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 6006 6038 4493
Size: 32 1481 32 4879
Fragments arranged by size:
4879 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3466 3478 3466
Size: 12 6412
PshAI GACnn'nnGTC
Cuts at: 1613 2993 1613
Size: 1380 5044
PsiI TTA'TAA
Cuts at: 3994 3994
Size: 6424
PspOMI G'GGCC_C
Cuts at: 2445 2445
Size: 6424
PspXI vC'TCGA_Gb
Cuts at: 3599 3599
Size: 6424
PstI C_TGCA'G
Cuts at: 830 2188 2679 3776 830
Size: 1358 491 1097 3478
Fragments arranged by size:
3478 1358 1097 491
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6424
PvuII CAG'CTG
Cuts at: 2198 2680 2774 3493 2198
Size: 482 94 719 5129
Fragments arranged by size:
5129 719 482 94
SalI G'TCGA_C
Cuts at: 1936 3766 1936
Size: 1830 4594
SapI GCTCTTCn'nnn_
Cuts at: 2391 2391
Size: 6424
SbfI CC_TGCA'GG
Cuts at: 3776 3776
Size: 6424
ScaI AGT'ACT
Cuts at: 4672 6384 4672
Size: 1712 4712
SfcI C'TryA_G
Cuts at: 826 1045 2184 2675 3772 4907 5588 5779 826
Size: 219 1139 491 1097 1135 681 191 1471
Fragments arranged by size:
1471 1139 1135 1097 681 491 219 191
SfoI GGC'GCC
Cuts at: 2551 2650 2551
Size: 99 6325
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6424
SmaI CCC'GGG
Cuts at: 1872 3751 1872
Size: 1879 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2269 2366 3428 3599 4528 5429
Size: 197 926 326 97 1062 171 929 901
Cuts at: 5429 5691 820
Size: 262 1553
Fragments arranged by size:
1553 1062 929 926 901 326 262 197
171 97
SnaBI TAC'GTA
Cuts at: 493 3721 4215 493
Size: 3228 494 2702
Fragments arranged by size:
3228 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6424
SphI G_CATG'C
Cuts at: 1132 3350 3782 1132
Size: 2218 432 3774
Fragments arranged by size:
3774 2218 432
SspI AAT'ATT
Cuts at: 5 52 4348 5
Size: 47 4296 2081
Fragments arranged by size:
4296 2081 47
StuI AGG'CCT
Cuts at: 1517 3251 1517
Size: 1734 4690
StyI C'CwwG_G
Cuts at: 513 1065 2022 3863 513
Size: 552 957 1841 3074
Fragments arranged by size:
3074 1841 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 2450 4451 4468 4621 4806 5251
Size: 1255 633 237 2001 17 153 185 445
Cuts at: 5251 325
Size: 1498
Fragments arranged by size:
2001 1498 1255 633 445 237 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2409 4670 6382
Size: 274 80 33 51 1443 432 2261 1712
Cuts at: 6382 96
Size: 138
Fragments arranged by size:
2261 1712 1443 432 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2700 2732 6384 6416 2700
Size: 32 3652 32 2708
Fragments arranged by size:
3652 2708 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6424
XbaI T'CTAG_A
Cuts at: 3760 3760
Size: 6424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3415 3415
Size: 6424
XhoI C'TCGA_G
Cuts at: 1943 3599 1943
Size: 1656 4768
XmaI C'CCGG_G
Cuts at: 1870 3749 1870
Size: 1879 4545
XmnI GAAnn'nnTTC
Cuts at: 4553 4553
Size: 6424
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4232 276
Size: 53 83 186 936 2698 2468
Fragments arranged by size:
2698 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI PacI PsrI RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI