Restriction map for
pFN21ASDA0789
(Linear) (Six-base) MAPSORT of: KIAA0789.seq Check: 6655 from: 1 to: 1695
KIAA0789.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 644 1695
Size: 644 1051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1468 1695
Size: 1468 227
AfeI AGC'GCT
Cuts at: 0 1558 1695
Size: 1558 137
AflIII A'CryG_T
Cuts at: 0 764 1061 1695
Size: 764 297 634
Fragments arranged by size:
764 634 297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 914 1046 1438 1695
Size: 914 132 392 257
Fragments arranged by size:
914 392 257 132
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 985 1017 1695
Size: 985 32 678
Fragments arranged by size:
985 678 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1054 1695
Size: 1054 641
ApaI G_GGCC'C
Cuts at: 0 426 1695
Size: 426 1269
ApaLI G'TGCA_C
Cuts at: 0 1432 1695
Size: 1432 263
ApoI r'AATT_y
Cuts at: 0 19 1695
Size: 19 1676
AvaI C'yCGr_G
Cuts at: 0 135 1576 1695
Size: 135 1441 119
Fragments arranged by size:
1441 135 119
BanI G'GyrC_C
Cuts at: 0 526 625 829 1695
Size: 526 99 204 866
Fragments arranged by size:
866 526 204 99
BanII G_rGCy'C
Cuts at: 0 342 426 1071 1568 1695
Size: 342 84 645 497 127
Fragments arranged by size:
645 497 342 127 84
BbeI G_GCGC'C
Cuts at: 0 530 629 1695
Size: 530 99 1066
Fragments arranged by size:
1066 530 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 90 1615 1695
Size: 90 1525 80
Fragments arranged by size:
1525 90 80
BbvCI CC'TCA_GC
Cuts at: 0 176 1695
Size: 176 1519
BfrBI ATG'CAT
Cuts at: 0 210 1695
Size: 210 1485
BglI GCCn_nnn'nGGC
Cuts at: 0 1276 1310 1493 1695
Size: 1276 34 183 202
Fragments arranged by size:
1276 202 183 34
BlpI GC'TnA_GC
Cuts at: 0 185 1695
Size: 185 1510
Bme1580I G_kGCm'C
Cuts at: 0 426 903 1436 1695
Size: 426 477 533 259
Fragments arranged by size:
533 477 426 259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 538 1695
Size: 351 187 1157
Fragments arranged by size:
1157 351 187
Bpu10I CC'TnA_GC
Cuts at: 0 176 1560 1695
Size: 176 1384 135
Fragments arranged by size:
1384 176 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 328 1390 1695
Size: 231 97 1062 305
Fragments arranged by size:
1062 305 231 97
BsaAI yAC'GTr
Cuts at: 0 1064 1695
Size: 1064 631
BsaBI GATnn'nnATC
Cuts at: 0 463 1695
Size: 463 1232
BsaHI Gr'CG_yC
Cuts at: 0 527 626 1235 1695
Size: 527 99 609 460
Fragments arranged by size:
609 527 460 99
BsaWI w'CCGG_w
Cuts at: 0 1568 1695
Size: 1568 127
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 446 934 1695
Size: 446 488 761
Fragments arranged by size:
761 488 446
BseYI C'CCAG_C
Cuts at: 0 85 171 1470 1491 1695
Size: 85 86 1299 21 204
Fragments arranged by size:
1299 204 86 85 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 777 930 1695
Size: 710 67 153 765
Fragments arranged by size:
765 710 153 67
BsiHKAI G_wGCw'C
Cuts at: 0 1436 1695
Size: 1436 259
Bsp1286I G_dGCh'C
Cuts at: 0 342 426 903 1071 1436 1568 1695
Size: 342 84 477 168 365 132 127
Fragments arranged by size:
477 365 342 168 132 127 84
BspEI T'CCGG_A
Cuts at: 0 1568 1695
Size: 1568 127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 251 668 1695
Size: 251 417 1027
Fragments arranged by size:
1027 417 251
BsrBI CCG'CTC
Cuts at: 0 870 1158 1695
Size: 870 288 537
Fragments arranged by size:
870 537 288
BsrDI GCAATG_nn'
Cuts at: 0 251 1028 1695
Size: 251 777 667
Fragments arranged by size:
777 667 251
BsrFI r'CCGG_y
Cuts at: 0 647 1514 1695
Size: 647 867 181
Fragments arranged by size:
867 647 181
BstAPI GCAn_nnn'nTGC
Cuts at: 0 617 823 836 1695
Size: 617 206 13 859
Fragments arranged by size:
859 617 206 13
BstEII G'GTnAC_C
Cuts at: 0 499 1695
Size: 499 1196
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1543 1695
Size: 1543 152
BstYI r'GATC_y
Cuts at: 0 551 1695
Size: 551 1144
BtgI C'CryG_G
Cuts at: 0 1375 1695
Size: 1375 320
BtsI GCAGTG_nn'
Cuts at: 0 698 1695
Size: 698 997
DraI TTT'AAA
Cuts at: 0 1149 1695
Size: 1149 546
DrdI GACnn_nn'nnGTC
Cuts at: 0 1462 1695
Size: 1462 233
EaeI y'GGCC_r
Cuts at: 0 2 543 1037 1088 1209 1302 1348 1695
Size: 2 541 494 51 121 93 46 347
Fragments arranged by size:
541 494 347 121 93 51 46 2
EarI CTCTTCn'nnn_
Cuts at: 0 368 1695
Size: 368 1327
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 1671 1695
Size: 25 1646 24
Fragments arranged by size:
1646 25 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 538 1468 1695
Size: 351 187 930 227
Fragments arranged by size:
930 351 227 187
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1450 1695
Size: 1450 245
EcoO109I rG'GnC_Cy
Cuts at: 0 423 1443 1455 1695
Size: 423 1020 12 240
Fragments arranged by size:
1020 423 240 12
FspI TGC'GCA
Cuts at: 0 1322 1695
Size: 1322 373
HaeII r_GCGC'y
Cuts at: 0 530 629 1560 1695
Size: 530 99 931 135
Fragments arranged by size:
931 530 135 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 854 886 1169 1201 1695
Size: 854 32 283 32 494
Fragments arranged by size:
854 494 283 32 32
HindIII A'AGCT_T
Cuts at: 0 307 1695
Size: 307 1388
KasI G'GCGC_C
Cuts at: 0 526 625 1695
Size: 526 99 1070
Fragments arranged by size:
1070 526 99
MscI TGG'CCA
Cuts at: 0 4 1039 1090 1350 1695
Size: 4 1035 51 260 345
Fragments arranged by size:
1035 345 260 51 4
MslI CAynn'nnrTG
Cuts at: 0 769 1630 1695
Size: 769 861 65
Fragments arranged by size:
861 769 65
MspA1I CmG'CkG
Cuts at: 0 27 130 157 175 657 751 813 1175
Size: 27 103 27 18 482 94 62 362
Cuts at: 1175 1470 1695
Size: 295 225
Fragments arranged by size:
482 362 295 225 103 94 62 27
27 18
NarI GG'CG_CC
Cuts at: 0 527 626 1695
Size: 527 99 1069
Fragments arranged by size:
1069 527 99
NsiI A_TGCA'T
Cuts at: 0 212 1695
Size: 212 1483
NspI r_CATG'y
Cuts at: 0 210 768 1327 1605 1695
Size: 210 558 559 278 90
Fragments arranged by size:
559 558 278 210 90
PciI A'CATG_T
Cuts at: 0 764 1695
Size: 764 931
PflMI CCAn_nnn'nTGG
Cuts at: 0 11 1054 1695
Size: 11 1043 641
Fragments arranged by size:
1043 641 11
PfoI T'CCnGG_A
Cuts at: 0 227 359 1695
Size: 227 132 1336
Fragments arranged by size:
1336 227 132
PmlI CAC'GTG
Cuts at: 0 1064 1695
Size: 1064 631
PpuMI rG'GwC_Cy
Cuts at: 0 1443 1455 1695
Size: 1443 12 240
Fragments arranged by size:
1443 240 12
PshAI GACnn'nnGTC
Cuts at: 0 970 1695
Size: 970 725
PspOMI G'GGCC_C
Cuts at: 0 422 1695
Size: 422 1273
PspXI vC'TCGA_Gb
Cuts at: 0 1576 1695
Size: 1576 119
PstI C_TGCA'G
Cuts at: 0 165 656 1695
Size: 165 491 1039
Fragments arranged by size:
1039 491 165
PvuII CAG'CTG
Cuts at: 0 175 657 751 1470 1695
Size: 175 482 94 719 225
Fragments arranged by size:
719 482 225 175 94
SapI GCTCTTCn'nnn_
Cuts at: 0 368 1695
Size: 368 1327
SfcI C'TryA_G
Cuts at: 0 161 652 1695
Size: 161 491 1043
Fragments arranged by size:
1043 491 161
SfoI GGC'GCC
Cuts at: 0 528 627 1695
Size: 528 99 1068
Fragments arranged by size:
1068 528 99
SmlI C'TyrA_G
Cuts at: 0 246 343 1405 1576 1695
Size: 246 97 1062 171 119
Fragments arranged by size:
1062 246 171 119 97
SphI G_CATG'C
Cuts at: 0 1327 1695
Size: 1327 368
StuI AGG'CCT
Cuts at: 0 1228 1695
Size: 1228 467
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 190 427 1695
Size: 190 237 1268
Fragments arranged by size:
1268 237 190
TatI w'GTAC_w
Cuts at: 0 386 1695
Size: 386 1309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 677 709 1695
Size: 677 32 986
Fragments arranged by size:
986 677 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1392 1695
Size: 1392 303
XhoI C'TCGA_G
Cuts at: 0 1576 1695
Size: 1576 119
Enzymes that do cut:
AccI AcuI AfeI AflIII AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BfrBI BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI
BstYI BtgI BtsI DraI DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HindIII KasI
MscI MslI MspA1I NarI NsiI NspI PciI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII
SapI SfcI SfoI SmlI SphI StuI TaqII TatI
TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BclI BglII BmgBI BmrI BmtI BplI BsaI BsaXI
BsiEI BsiWI BsmI BsmBI BspHI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EagI
EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HpaI
KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PmeI PpiI PsiI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StyI SwaI Tth111I XbaI XmaI XmnI
ZraI