Restriction map for
pFN21ASDA0785
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# NO CUT ENZYMES ( 87 enzymes) #
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AccI AclI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI AspEI AviII AvrII BciVI
BclI BfaI BfrI BlnI BsaBI
BsiWI BsmI BspDI BspHI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRV FbaI FspI
HincII HindII HindIII HpaI KspI
MaeI MamI MfeI MluI MseI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 847 GACGTC GACGT^C
Acc65I 1244 GGTACC G^GTACC
AccIII 169 TCCGGA T^CCGGA
AcsI 220 GAATTC [GA]^AATT[CT]
Alw44I 856 GTGCAC G^TGCAC
AlwNI 370 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 856 GTGCAC G^TGCAC
ApoI 220 GAATTC [GA]^AATT[CT]
Asp718I 1244 GGTACC G^GTACC
AspI 1516 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1867 GGATCC G^GATCC
BbeI 661 GGCGCC GGCGC^C
BbrPI 1918 CACGTG CAC^GTG
BbvCI 1546 GCTGAGG CCTCAGC(-5/-2)
BcgI 815 GCAAGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1918 CACGTG [CT]AC^GT[GA]
BsaWI 169 TCCGGA [AT]^CCGG[AT]
BseAI 169 TCCGGA T^CCGGA
BsgI 1276 CTGCAC GTGCAG(16/14)
Bsp1407I 986 TGTACA T^GTACA
BspEI 169 TCCGGA T^CCGGA
BsrBI 1465 GAGCGG CCGCTC(-3/-3)
BsrGI 986 TGTACA T^GTACA
DraIII 573 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2004 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 220 GAATTC G^AATTC
EcoT22I 111 ATGCAT ATGCA^T
FseI 339 GGCCGGCC GGCCGG^CC
KasI 661 GGCGCC G^GCGCC
KpnI 1244 GGTACC GGTAC^C
MroI 169 TCCGGA T^CCGGA
NarI 661 GGCGCC GG^CGCC
NcoI 680 CCATGG C^CATGG
NsiI 111 ATGCAT ATGCA^T
PflFI 1516 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1918 CACGTG CAC^GTG
PmlI 1918 CACGTG CAC^GTG
SexAI 574 ACCTGGT A^CC[AT]GGT
SfiI 330 GGCCGTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 661 GGCGCC GGC^GCC
SmaI 313 CCCGGG CCC^GGG
SspBI 986 TGTACA T^GTACA
StuI 902 AGGCCT AGG^CCT
Tsp509I 221 AATT ^AATT
Tth111I 1516 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 313 CCCGGG C^CCGGG
XmaI 313 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflIII 596 ACATGT A^C[GA][CT]GT
1397 ACATGT
Asp700I 78 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
699 GAAGACCTTC
BglI 331 GCCGTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1658 GCCAGACAGGC
BglII 1556 AGATCT A^GATCT
1649 AGATCT
BlpI 1329 GCTCAGC GC^T[ACGT]AGC
1713 GCTGAGC
Bpu1102I 1329 GCTCAGC GC^T[ACGT]AGC
1713 GCTGAGC
BsaI 733 GAGACC GGTCTC(1/5)
1312 GAGACC
BspLU11I 596 ACATGT A^CATGT
1397 ACATGT
BsrI 951 ACTGG ACTGG(1/-1)
1823 ACTGG
BstXI 1253 CCATCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1415 CCACCTTCCTGG
CelII 1329 GCTCAGC GC^T[ACGT]AGC
1713 GCTGAGC
DraII 1505 AGGGCCT [GA]G^G[ACGT]CC[CT]
2000 GGGGCCT
EcoO109I 1505 AGGGCCT [GA]G^G[ACGT]CC[CT]
2000 GGGGCCT
MaeIII 631 GTCAC ^GT[ACGT]AC
1831 GTGAC
PleI 1318 GACTC GAGTC(4/5)
298 GAGTC
ScaI 959 AGTACT AGT^ACT
1820 AGTACT
SmlI 1090 CTCAAG C^T[CT][GA]AG
1762 CTCAAG
SphI 1031 GCATGC GCATG^C
1692 GCATGC
TfiI 612 GAATC G^A[AT]TC
1482 GAATC
Tsp45I 631 GTCAC ^GT[GC]AC
1831 GTGAC
XmnI 78 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
699 GAAGACCTTC
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EagI EarI EclXI Eco52I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI TspRI TthHB8I
Van91I XcmI XhoII