Restriction map for
pFN21ASDA0785
(Linear) (Six-base) MAPSORT of: KIAA0785.seq Check: 1309 from: 1 to: 2082
KIAA0785.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1206 1464 1920 2007 2082
Size: 1206 258 456 87 75
Fragments arranged by size:
1206 456 258 87 75
AatII G_ACGT'C
Cuts at: 0 851 2082
Size: 851 1231
Acc65I G'GTAC_C
Cuts at: 0 1244 2082
Size: 1244 838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 310 478 574 718 2082
Size: 58 252 168 96 144 1364
Fragments arranged by size:
1364 252 168 144 96 58
AflIII A'CryG_T
Cuts at: 0 596 1397 2082
Size: 596 801 685
Fragments arranged by size:
801 685 596
AleI CACnn'nnGTG
Cuts at: 0 745 2082
Size: 745 1337
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1087 1119 2005 2037 2082
Size: 1087 32 886 32 45
Fragments arranged by size:
1087 886 45 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 375 2082
Size: 375 1707
ApaLI G'TGCA_C
Cuts at: 0 856 2082
Size: 856 1226
ApoI r'AATT_y
Cuts at: 0 220 2082
Size: 220 1862
AvaI C'yCGr_G
Cuts at: 0 214 295 313 1387 1570 1975 2082
Size: 214 81 18 1074 183 405 107
Fragments arranged by size:
1074 405 214 183 107 81 18
BamHI G'GATC_C
Cuts at: 0 1867 2082
Size: 1867 215
BanI G'GyrC_C
Cuts at: 0 661 711 1244 1566 2082
Size: 661 50 533 322 516
Fragments arranged by size:
661 533 516 322 50
BanII G_rGCy'C
Cuts at: 0 25 440 688 1199 1331 2082
Size: 25 415 248 511 132 751
Fragments arranged by size:
751 511 415 248 132 25
BbeI G_GCGC'C
Cuts at: 0 665 2082
Size: 665 1417
BbsI GAAGACnn'nnnn_
Cuts at: 0 466 706 753 783 1760 2082
Size: 466 240 47 30 977 322
Fragments arranged by size:
977 466 322 240 47 30
BbvCI CC'TCA_GC
Cuts at: 0 1547 2082
Size: 1547 535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 804 838 2082
Size: 804 34 1244
Fragments arranged by size:
1244 804 34
BfrBI ATG'CAT
Cuts at: 0 113 2082
Size: 113 1969
BglI GCCn_nnn'nGGC
Cuts at: 0 337 1664 2082
Size: 337 1327 418
Fragments arranged by size:
1327 418 337
BglII A'GATC_T
Cuts at: 0 1556 1649 2082
Size: 1556 93 433
Fragments arranged by size:
1556 433 93
BlpI GC'TnA_GC
Cuts at: 0 1330 1714 2082
Size: 1330 384 368
Fragments arranged by size:
1330 384 368
Bme1580I G_kGCm'C
Cuts at: 0 716 860 1571 2082
Size: 716 144 711 511
Fragments arranged by size:
716 711 511 144
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1335 2082
Size: 1335 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 220 1177 1224 2082
Size: 37 183 957 47 858
Fragments arranged by size:
957 858 183 47 37
Bpu10I CC'TnA_GC
Cuts at: 0 1547 1853 2082
Size: 1547 306 229
Fragments arranged by size:
1547 306 229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1075 1747 2082
Size: 1075 672 335
Fragments arranged by size:
1075 672 335
BsaI GGTCTCn'nnnn_
Cuts at: 0 727 1306 2082
Size: 727 579 776
Fragments arranged by size:
776 727 579
BsaAI yAC'GTr
Cuts at: 0 1920 2082
Size: 1920 162
BsaHI Gr'CG_yC
Cuts at: 0 662 848 1384 2039 2082
Size: 662 186 536 655 43
Fragments arranged by size:
662 655 536 186 43
BsaWI w'CCGG_w
Cuts at: 0 169 2082
Size: 169 1913
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 406 436 1036 1066 1087 1117 2005 2035
Size: 406 30 600 30 21 30 888 30
Cuts at: 2035 2082
Size: 47
Fragments arranged by size:
888 600 406 47 30 30 30 30
21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 618 1588 1723 2082
Size: 618 970 135 359
Fragments arranged by size:
970 618 359 135
BseYI C'CCAG_C
Cuts at: 0 305 390 417 487 921 2082
Size: 305 85 27 70 434 1161
Fragments arranged by size:
1161 434 305 85 70 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1261 2082
Size: 1261 821
BsiEI CG_ry'CG
Cuts at: 0 383 467 1543 2082
Size: 383 84 1076 539
Fragments arranged by size:
1076 539 383 84
BsiHKAI G_wGCw'C
Cuts at: 0 25 752 838 860 1069 1199 1331 2082
Size: 25 727 86 22 209 130 132 751
Fragments arranged by size:
751 727 209 132 130 86 25 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1056 1375 1864 2082
Size: 1056 319 489 218
Fragments arranged by size:
1056 489 319 218
Bsp1286I G_dGCh'C
Cuts at: 0 25 440 688 716 752 838 860 1069
Size: 25 415 248 28 36 86 22 209
Cuts at: 1069 1199 1331 1571 2082
Size: 130 132 240 511
Fragments arranged by size:
511 415 248 240 209 132 130 86
36 28 25 22
BspEI T'CCGG_A
Cuts at: 0 169 2082
Size: 169 1913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1206 1464 1920 2007 2082
Size: 1206 258 456 87 75
Fragments arranged by size:
1206 456 258 87 75
BsrBI CCG'CTC
Cuts at: 0 1467 2082
Size: 1467 615
BsrFI r'CCGG_y
Cuts at: 0 340 889 1538 1542 1901 2082
Size: 340 549 649 4 359 181
Fragments arranged by size:
649 549 359 340 181 4
BsrGI T'GTAC_A
Cuts at: 0 986 2082
Size: 986 1096
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1260 1422 2082
Size: 1260 162 660
Fragments arranged by size:
1260 660 162
BstYI r'GATC_y
Cuts at: 0 1556 1649 1867 2082
Size: 1556 93 218 215
Fragments arranged by size:
1556 218 215 93
BtgI C'CryG_G
Cuts at: 0 345 680 977 2082
Size: 345 335 297 1105
Fragments arranged by size:
1105 345 335 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1095 1335 2082
Size: 1095 240 747
Fragments arranged by size:
1095 747 240
BtsI GCAGTG_nn'
Cuts at: 0 127 860 1026 2082
Size: 127 733 166 1056
Fragments arranged by size:
1056 733 166 127
DraIII CAC_nnn'GTG
Cuts at: 0 578 2082
Size: 578 1504
EaeI y'GGCC_r
Cuts at: 0 329 342 380 464 563 638 980 1073
Size: 329 13 38 84 99 75 342 93
Cuts at: 1073 1220 1337 1412 1540 1643 1835 2082
Size: 147 117 75 128 103 192 247
Fragments arranged by size:
342 329 247 192 147 128 117 103
99 93 84 75 75 38 13
EagI C'GGCC_G
Cuts at: 0 380 464 1540 2082
Size: 380 84 1076 542
Fragments arranged by size:
1076 542 380 84
EarI CTCTTCn'nnn_
Cuts at: 0 713 1490 1679 1795 1867 2082
Size: 713 777 189 116 72 215
Fragments arranged by size:
777 713 215 189 116 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 745 1446 1860 1984 2082
Size: 745 701 414 124 98
Fragments arranged by size:
745 701 414 124 98
EcoICRI GAG'CTC
Cuts at: 0 23 1197 1329 2082
Size: 23 1174 132 753
Fragments arranged by size:
1174 753 132 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 58 220 310 478 574 718 1177
Size: 37 21 162 90 168 96 144 459
Cuts at: 1177 1224 2082
Size: 47 858
Fragments arranged by size:
858 459 168 162 144 96 90 47
37 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2008 2082
Size: 2008 74
EcoO109I rG'GnC_Cy
Cuts at: 0 1506 2001 2082
Size: 1506 495 81
Fragments arranged by size:
1506 495 81
EcoRI G'AATT_C
Cuts at: 0 220 2082
Size: 220 1862
FseI GG_CCGG'CC
Cuts at: 0 344 2082
Size: 344 1738
HaeII r_GCGC'y
Cuts at: 0 665 785 931 1089 1892 2082
Size: 665 120 146 158 803 190
Fragments arranged by size:
803 665 190 158 146 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 597 629 1216 1248 2082
Size: 597 32 587 32 834
Fragments arranged by size:
834 597 587 32 32
KasI G'GCGC_C
Cuts at: 0 661 2082
Size: 661 1421
KpnI G_GTAC'C
Cuts at: 0 1248 2082
Size: 1248 834
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 2082
Size: 206 1876
MscI TGG'CCA
Cuts at: 0 982 1075 1339 1414 2082
Size: 982 93 264 75 668
Fragments arranged by size:
982 668 264 93 75
MslI CAynn'nnrTG
Cuts at: 0 148 595 745 988 1036 1258 2082
Size: 148 447 150 243 48 222 824
Fragments arranged by size:
824 447 243 222 150 148 48
MspA1I CmG'CkG
Cuts at: 0 692 1272 2045 2082
Size: 692 580 773 37
Fragments arranged by size:
773 692 580 37
NaeI GCC'GGC
Cuts at: 0 342 891 1544 2082
Size: 342 549 653 538
Fragments arranged by size:
653 549 538 342
NarI GG'CG_CC
Cuts at: 0 662 2082
Size: 662 1420
NcoI C'CATG_G
Cuts at: 0 680 2082
Size: 680 1402
NgoMIV G'CCGG_C
Cuts at: 0 340 889 1542 2082
Size: 340 549 653 540
Fragments arranged by size:
653 549 540 340
NsiI A_TGCA'T
Cuts at: 0 115 2082
Size: 115 1967
NspI r_CATG'y
Cuts at: 0 600 864 993 1035 1284 1401 1641 1696
Size: 600 264 129 42 249 117 240 55
Cuts at: 1696 2082
Size: 386
Fragments arranged by size:
600 386 264 249 240 129 117 55
42
PciI A'CATG_T
Cuts at: 0 596 1397 2082
Size: 596 801 685
Fragments arranged by size:
801 685 596
PflMI CCAn_nnn'nTGG
Cuts at: 0 651 1169 1704 2082
Size: 651 518 535 378
Fragments arranged by size:
651 535 518 378
PfoI T'CCnGG_A
Cuts at: 0 539 1421 2082
Size: 539 882 661
Fragments arranged by size:
882 661 539
PmlI CAC'GTG
Cuts at: 0 1920 2082
Size: 1920 162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1087 1119 2005 2037 2082
Size: 1087 32 886 32 45
Fragments arranged by size:
1087 886 45 32 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 610 642 2082
Size: 610 32 1440
Fragments arranged by size:
1440 610 32
PstI C_TGCA'G
Cuts at: 0 122 1085 1205 1817 2082
Size: 122 963 120 612 265
Fragments arranged by size:
963 612 265 122 120
SacI G_AGCT'C
Cuts at: 0 25 1199 1331 2082
Size: 25 1174 132 751
Fragments arranged by size:
1174 751 132 25
ScaI AGT'ACT
Cuts at: 0 961 1822 2082
Size: 961 861 260
Fragments arranged by size:
961 861 260
SexAI A'CCwGG_T
Cuts at: 0 574 2082
Size: 574 1508
SfcI C'TryA_G
Cuts at: 0 118 1081 1201 1813 2082
Size: 118 963 120 612 269
Fragments arranged by size:
963 612 269 120 118
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 337 2082
Size: 337 1745
SfoI GGC'GCC
Cuts at: 0 663 2082
Size: 663 1419
SmaI CCC'GGG
Cuts at: 0 315 2082
Size: 315 1767
SmlI C'TyrA_G
Cuts at: 0 1090 1762 2082
Size: 1090 672 320
Fragments arranged by size:
1090 672 320
SphI G_CATG'C
Cuts at: 0 1035 1696 2082
Size: 1035 661 386
Fragments arranged by size:
1035 661 386
SrfI GCCC'GGGC
Cuts at: 0 315 2082
Size: 315 1767
StuI AGG'CCT
Cuts at: 0 904 2082
Size: 904 1178
StyI C'CwwG_G
Cuts at: 0 509 680 785 1322 1340 1355 1502 1805
Size: 509 171 105 537 18 15 147 303
Cuts at: 1805 2027 2082
Size: 222 55
Fragments arranged by size:
537 509 303 222 171 147 105 55
18 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1330 2082
Size: 1330 752
TatI w'GTAC_w
Cuts at: 0 497 959 986 1820 2082
Size: 497 462 27 834 262
Fragments arranged by size:
834 497 462 262 27
Tth111I GACn'n_nGTC
Cuts at: 0 1519 2082
Size: 1519 563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1185 1257 1509 1738 2082
Size: 1185 72 252 229 344
Fragments arranged by size:
1185 344 252 229 72
XmaI C'CCGG_G
Cuts at: 0 313 2082
Size: 313 1769
XmnI GAAnn'nnTTC
Cuts at: 0 82 703 2082
Size: 82 621 1379
Fragments arranged by size:
1379 621 82
ZraI GAC'GTC
Cuts at: 0 849 2082
Size: 849 1233
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AleI AloI AlwNI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrGI BstXI BstYI BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmlI PpiI PsrI
PstI SacI ScaI SexAI SfcI SfiI SfoI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI Tth111I
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AlfI ApaI
AscI AseI AsiSI AvrII BaeI BciVI BclI BmgBI
BmrI BmtI BsaBI BsiWI BsmI BspHI BsrDI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DrdI EcoRV FalI FspI FspAI HincII HindIII
HpaI MfeI MluI NdeI NheI NotI NruI PacI
PasI PmeI PpuMI PshAI PsiI PspOMI PspXI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI SgfI
SgrAI SnaBI SpeI SspI SwaI TstI XbaI XhoI