Restriction map for
pFN21ASDA0781
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI AhdI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BbrPI BclI BglII
BlnI BsaAI BsaBI BsiEI BsiWI
Bsp1407I BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MluNI MscI MslI MunI NcoI
NdeI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SalI SapI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 701 TCCGGA T^CCGGA
AcyI 1896 GGCGCC G[GA]^CG[CT]C
AflII 1603 CTTAAG C^TTAAG
Alw44I 415 GTGCAC G^TGCAC
AocI 1939 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2557 AGCGCT AGC^GCT
ApaLI 415 GTGCAC G^TGCAC
Asp700I 1497 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2745 GGATCC G^GATCC
BbeI 1896 GGCGCC GGCGC^C
BcgI 20 CGAGGCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2598 GTATCC GTATCC(6/5)
BfrI 1603 CTTAAG C^TTAAG
BlpI 1081 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1081 GCTGAGC GC^T[ACGT]AGC
BsaHI 1896 GGCGCC G[GA]^CG[CT]C
BseAI 701 TCCGGA T^CCGGA
BsgI 1849 GTGCAG GTGCAG(16/14)
BspDI 151 ATCGAT AT^CGAT
BspEI 701 TCCGGA T^CCGGA
BsrBI 1397 CCGCTC CCGCTC(-3/-3)
BsrDI 1077 CATTGC GCAATG(2/0)
BstXI 1466 CCAATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2745 GGATCC [GA]^GATC[CT]
Bsu36I 1939 CCTCAGG CC^T[ACGT]AGG
CelII 1081 GCTGAGC GC^T[ACGT]AGC
ClaI 151 ATCGAT AT^CGAT
CpoI 41 CGGTCCG CG^G[AT]CCG
DraI 499 TTTAAA TTT^AAA
Eco47III 2557 AGCGCT AGC^GCT
Eco81I 1939 CCTCAGG CC^T[ACGT]AGG
EcoRI 1829 GAATTC G^AATTC
EcoT22I 1129 ATGCAT ATGCA^T
Hin1I 1896 GGCGCC G[GA]^CG[CT]C
KasI 1896 GGCGCC G^GCGCC
KspI 2113 CCGCGG CCGC^GG
MaeII 1184 ACGT A^CGT
MflI 2745 GGATCC [GA]^GATC[CT]
MroI 701 TCCGGA T^CCGGA
NaeI 1899 GCCGGC GCC^GGC
NarI 1896 GGCGCC GG^CGCC
NgoMIV 1899 GCCGGC G^CCGGC
NheI 1927 GCTAGC G^CTAGC
NsiI 1129 ATGCAT ATGCA^T
PflMI 1204 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 41 CGGTCCG CG^G[AT]CCG
SacII 2113 CCGCGG CCGC^GG
SfoI 1896 GGCGCC GGC^GCC
SgrAI 1898 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2423 CCCGGG CCC^GGG
Van91I 1204 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1833 TCTAGA T^CTAGA
XhoII 2745 GGATCC [GA]^GATC[CT]
XmaCI 2423 CCCGGG C^CCGGG
XmaI 2423 CCCGGG C^CCGGG
XmnI 1497 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 34 CGCG CG^CG
2114 CGCG
AvaI 18 CCCGAG C^[CT]CG[GA]G
2423 CCCGGG
BanI 113 GGCACC G^G[CT][GA]CC
1896 GGCGCC
BbsI 1390 GAAGAC GAAGAC(2/6)
1588 GAAGAC
BbvCI 1909 CCTCAGC CCTCAGC(-5/-2)
2370 CCTCAGC
BglI 1405 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1681 GCCATGCAGGC
BpuAI 1390 GAAGAC GAAGAC(2/6)
1588 GAAGAC
BsmBI 1971 CGTCTC CGTCTC(1/5)
1440 GAGACG
BsmI 1131 GCATTC GAATGC(1/-1)
1231 GCATTC
BsoBI 18 CCCGAG C^[CT]CG[GA]G
2423 CCCGGG
BspMI 2036 ACCTGC ACCTGC(4/8)
894 GCAGGT
BstAPI 894 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2127 GCAGCAGCTGC
BstUI 34 CGCG CG^CG
2114 CGCG
DsaI 2011 CCACGG C^C[GA][CT]GG
2113 CCGCGG
EaeI 333 TGGCCG [CT]^GGCC[GA]
1053 TGGCCG
EarI 662 CTCTTC CTCTTC(1/4)
1243 GAAGAG
EcoT14I 918 CCTTGG C^C[AT][AT]GG
1097 CCAAGG
Ksp632I 662 CTCTTC CTCTTC(1/4)
1243 GAAGAG
MvnI 34 CGCG CG^CG
2114 CGCG
PpuMI 1571 GGGACCT [GA]G^G[AT]CC[CT]
1919 AGGACCT
StuI 1190 AGGCCT AGG^CCT
2192 AGGCCT
StyI 918 CCTTGG C^C[AT][AT]GG
1097 CCAAGG
TaqI 65 TCGA T^CGA
152 TCGA
TthHB8I 65 TCGA T^CGA
152 TCGA
XcmI 125 CCAAGACGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1331 CCAGCAACATGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI