(Circular) (Six-base) MAPSORT of: pFN21ASDA0781.seq Check: 7756 from: 1 to: 7507 pFN21ASDA0781 7507 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5317 278 Size: 53 83 186 936 3781 2468 Fragments arranged by size: 3781 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4850 1937 Size: 2913 4594 Acc65I G'GTAC_C Cuts at: 4828 4828 Size: 7507 AclI AA'CG_TT Cuts at: 1563 5634 6007 1563 Size: 4071 373 3063 Fragments arranged by size: 4071 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2394 3745 3812 4178 4403 5570 6954 7222 Size: 762 1351 67 366 225 1167 1384 268 Cuts at: 7222 1632 Size: 1917 Fragments arranged by size: 1917 1384 1351 1167 762 366 268 225 67 AfeI AGC'GCT Cuts at: 4582 4582 Size: 7507 AflII C'TTAA_G Cuts at: 820 1017 3626 820 Size: 197 2609 4701 Fragments arranged by size: 4701 2609 197 AflIII A'CryG_T Cuts at: 1203 6406 7383 1203 Size: 5203 977 1327 Fragments arranged by size: 5203 1327 977 AgeI A'CCGG_T Cuts at: 6338 6338 Size: 7507 AhdI GACnn_n'nnGTC Cuts at: 6236 6236 Size: 7507 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7507 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3132 1184 Size: 1948 5559 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4155 4187 4155 Size: 32 7475 AlwNI CAG_nnn'CTG Cuts at: 2923 3233 3349 3715 3835 4045 4060 4156 4273 Size: 310 116 366 120 210 15 96 117 Cuts at: 4273 4342 4426 4532 5250 6822 2923 Size: 69 84 106 718 1572 3608 Fragments arranged by size: 3608 1572 718 366 310 210 120 117 116 106 96 84 69 15 ApaLI G'TGCA_C Cuts at: 2438 5564 6720 2438 Size: 3126 1156 3225 Fragments arranged by size: 3225 3126 1156 ApoI r'AATT_y Cuts at: 1493 2331 2387 2515 3522 3852 4816 5042 5194 Size: 838 56 128 1007 330 964 226 152 Cuts at: 5194 7303 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1007 964 838 330 226 152 128 56 AseI AT'TA_AT Cuts at: 160 1032 6061 160 Size: 872 5029 1606 Fragments arranged by size: 5029 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7507 AvaI C'yCGr_G Cuts at: 1870 1943 2041 4446 4832 1870 Size: 73 98 2405 386 4545 Fragments arranged by size: 4545 2405 386 98 73 BamHI G'GATC_C Cuts at: 4768 4837 5204 4768 Size: 69 367 7071 Fragments arranged by size: 7071 367 69 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2136 3919 4828 6283 618 Size: 325 217 627 349 1783 909 1455 1842 Fragments arranged by size: 1842 1783 1455 909 627 349 325 217 BanII G_rGCy'C Cuts at: 1391 2665 3141 3722 4250 4349 4639 4826 1391 Size: 1274 476 581 528 99 290 187 4072 Fragments arranged by size: 4072 1274 581 528 476 290 187 99 BbeI G_GCGC'C Cuts at: 3923 3923 Size: 7507 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3420 3618 928 Size: 971 1521 198 4817 Fragments arranged by size: 4817 1521 971 198 BbvCI CC'TCA_GC Cuts at: 3933 4394 3933 Size: 461 7046 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2032 2066 5698 5732 1903 Size: 34 95 34 3632 34 3678 Fragments arranged by size: 3678 3632 95 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4632 5399 6609 4632 Size: 767 1210 5530 Fragments arranged by size: 5530 1210 767 BclI T'GATC_A Cuts at: 1548 1548 Size: 7507 BfrBI ATG'CAT Cuts at: 3154 3154 Size: 7507 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3434 3710 6118 Size: 107 122 71 631 605 1762 276 2408 Cuts at: 6118 136 Size: 1525 Fragments arranged by size: 2408 1762 1525 631 605 276 122 107 71 BglII A'GATC_T Cuts at: 7502 7502 Size: 7507 BlpI GC'TnA_GC Cuts at: 3105 4924 3105 Size: 1819 5688 Bme1580I G_kGCm'C Cuts at: 1790 2096 2442 5568 6724 1790 Size: 306 346 3126 1156 2573 Fragments arranged by size: 3126 2573 1156 346 306 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7507 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3050 3137 4048 Size: 416 53 83 399 24 1633 87 911 Cuts at: 4048 5238 6196 442 Size: 1190 958 1753 Fragments arranged by size: 1753 1633 1190 958 911 416 399 87 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3954 1056 Size: 2898 4609 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4381 4714 4381 Size: 333 7174 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2644 3410 3735 3923 4058 4067 4157 4376 Size: 1405 766 325 188 135 9 90 219 Cuts at: 4376 4684 6167 1239 Size: 308 1483 2579 Fragments arranged by size: 2579 1483 1405 766 325 308 219 188 135 90 9 Bpu10I CC'TnA_GC Cuts at: 3933 4343 4394 3933 Size: 410 51 7046 Fragments arranged by size: 7046 410 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2225 4166 5632 6497 6795 2225 Size: 1941 1466 865 298 2937 Fragments arranged by size: 2937 1941 1466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2276 2851 3362 3682 3753 4043 6170 Size: 617 777 575 511 320 71 290 2127 Cuts at: 6170 882 Size: 2219 Fragments arranged by size: 2219 2127 777 617 575 511 320 290 71 BsaAI yAC'GTr Cuts at: 493 1204 4804 5298 493 Size: 711 3600 494 2702 Fragments arranged by size: 3600 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7501 7501 Size: 7507 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3920 5314 5696 6331 Size: 53 83 186 936 2387 1394 382 635 Cuts at: 6331 275 Size: 1451 Fragments arranged by size: 2387 1451 1394 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2139 2724 3898 4439 5940 6338 6612 6759 7170 Size: 585 1174 541 1501 398 274 147 411 Cuts at: 7170 2139 Size: 2476 Fragments arranged by size: 2476 1501 1174 585 541 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3530 3560 4040 4070 4155 Size: 30 165 30 2127 30 480 30 85 Cuts at: 4155 4185 1178 Size: 30 4500 Fragments arranged by size: 4500 2127 480 165 85 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3179 3921 4446 4449 1186 Size: 1993 742 525 3 4244 Fragments arranged by size: 4244 1993 742 525 3 BseYI C'CCAG_C Cuts at: 2098 2128 3353 3700 4023 4082 4335 4537 4638 Size: 30 1225 347 323 59 253 202 101 Cuts at: 4638 6710 7206 2098 Size: 2072 496 2399 Fragments arranged by size: 2399 2072 1225 496 347 323 253 202 101 59 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3893 1163 Size: 573 2157 4777 Fragments arranged by size: 4777 2157 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4957 5718 6746 1540 Size: 275 186 2956 761 1028 2301 Fragments arranged by size: 2956 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2442 2665 2760 3722 4250 5568 5653 6724 2442 Size: 223 95 962 528 1318 85 1071 3225 Fragments arranged by size: 3225 1318 1071 962 528 223 95 85 BsmI GAATG_Cn' Cuts at: 3154 3254 5018 5111 3154 Size: 100 1764 93 5550 Fragments arranged by size: 5550 1764 100 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3457 4000 3457 Size: 543 6964 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2442 2665 2760 3141 3722 4250 Size: 399 306 346 223 95 381 581 528 Cuts at: 4250 4349 4639 4826 5568 5653 6724 1391 Size: 99 290 187 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 581 528 399 381 346 306 290 223 187 99 95 85 BspEI T'CCGG_A Cuts at: 2724 7170 2724 Size: 4446 3061 BspHI T'CATG_A Cuts at: 5396 7126 5396 Size: 1730 5777 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2908 4068 4862 844 Size: 2064 1160 794 3489 Fragments arranged by size: 3489 2064 1160 794 BsrBI CCG'CTC Cuts at: 1394 1823 3422 5394 1394 Size: 429 1599 1972 3507 Fragments arranged by size: 3507 1972 1599 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3099 6002 6176 65 Size: 1183 1851 2903 174 1396 Fragments arranged by size: 2903 1851 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 2358 3922 6151 6338 1700 Size: 110 252 296 1564 2229 187 2869 Fragments arranged by size: 2869 2229 1564 296 252 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7507 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7507 BssSI C'ACGA_G Cuts at: 5567 6579 5567 Size: 1012 6495 BstAPI GCAn_nnn'nTGC Cuts at: 794 2923 4156 794 Size: 2129 1233 4145 Fragments arranged by size: 4145 2129 1233 BstBI TT'CG_AA Cuts at: 2007 6311 2007 Size: 4304 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7507 BstXI CCAn_nnnn'nTGG Cuts at: 2029 3496 2029 Size: 1467 6040 BstYI r'GATC_y Cuts at: 1972 4768 4837 5204 5589 5606 7058 7144 7156 Size: 2796 69 367 385 17 1452 86 12 Cuts at: 7156 7502 1972 Size: 346 1977 Fragments arranged by size: 2796 1977 1452 385 367 346 86 69 17 12 Bsu36I CC'TnA_GG Cuts at: 3963 3963 Size: 7507 BtgI C'CryG_G Cuts at: 513 1065 2022 4034 4136 513 Size: 552 957 2012 102 3884 Fragments arranged by size: 3884 2012 957 552 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6768 Fragments arranged by size: 6768 683 56 BtsI GCAGTG_nn' Cuts at: 2202 3347 5023 5817 5837 2202 Size: 1145 1676 794 20 3872 Fragments arranged by size: 3872 1676 1145 794 20 ClaI AT'CG_AT Cuts at: 1554 2175 1554 Size: 621 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7472 DraI TTT'AAA Cuts at: 2524 4811 2524 Size: 2287 5220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6514 809 Size: 507 5198 1802 Fragments arranged by size: 5198 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2356 3076 4954 Size: 54 1425 50 275 18 526 720 1878 Cuts at: 4954 5843 7472 8 Size: 889 1629 43 Fragments arranged by size: 1878 1629 1425 889 720 526 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4954 1537 Size: 275 3142 4090 Fragments arranged by size: 4090 3142 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2691 3261 5437 1353 Size: 1338 570 2176 3423 Fragments arranged by size: 3423 2176 1338 570 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2048 3260 6094 6468 6614 387 Size: 1064 138 459 1212 2834 374 146 1280 Fragments arranged by size: 2834 1280 1212 1064 459 374 146 138 EcoICRI GAG'CTC Cuts at: 2663 3720 4248 2663 Size: 1057 528 5922 Fragments arranged by size: 5922 1057 528 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2394 2644 3410 3735 3745 3812 3923 Size: 393 762 250 766 325 10 67 111 Cuts at: 3923 4058 4067 4157 4178 4376 4403 4684 5570 Size: 135 9 90 21 198 27 281 886 Cuts at: 5570 6167 6954 7222 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 886 787 766 762 597 393 325 281 268 250 198 135 111 90 67 27 21 10 9 EcoNI CCTnn'n_nnAGG Cuts at: 3194 3513 3797 3194 Size: 319 284 6904 Fragments arranged by size: 6904 319 284 EcoO109I rG'GnC_Cy Cuts at: 3595 3943 4315 4693 3595 Size: 348 372 378 6409 Fragments arranged by size: 6409 378 372 348 EcoRI G'AATT_C Cuts at: 3852 4816 5194 3852 Size: 964 378 6165 Fragments arranged by size: 6165 964 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2128 4204 4602 2128 Size: 2076 398 5033 Fragments arranged by size: 5033 2076 398 FspI TGC'GCA Cuts at: 6013 6013 Size: 7507 HaeII r_GCGC'y Cuts at: 1678 3031 3923 4584 6654 1678 Size: 1353 892 661 2070 2531 Fragments arranged by size: 2531 2070 1353 892 661 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2850 2882 3171 3203 3530 Size: 32 135 32 1278 32 289 32 327 Cuts at: 3530 3562 3566 3598 6148 6180 6222 6254 1373 Size: 32 4 32 2550 32 42 32 2626 Fragments arranged by size: 2626 2550 1278 327 289 135 42 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 4851 5097 669 Size: 988 281 2913 246 3079 Fragments arranged by size: 3079 2913 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7507 HpaI GTT'AAC Cuts at: 5097 5097 Size: 7507 KasI G'GCGC_C Cuts at: 3919 3919 Size: 7507 KpnI G_GTAC'C Cuts at: 4832 4832 Size: 7507 MfeI C'AATT_G Cuts at: 5106 5106 Size: 7507 MluI A'CGCG_T Cuts at: 7383 7383 Size: 7507 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2408 3228 6621 6805 1305 Size: 1103 820 3393 184 2007 Fragments arranged by size: 3393 2007 1103 820 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5685 Fragments arranged by size: 5685 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 5465 5824 5983 518 Size: 648 861 3438 359 159 2042 Fragments arranged by size: 3438 2042 861 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2188 2614 3456 3957 4027 4138 4156 4273 Size: 582 426 842 501 70 111 18 117 Cuts at: 4273 4923 5600 6748 6993 1606 Size: 650 677 1148 245 2120 Fragments arranged by size: 2120 1148 842 677 650 582 501 426 245 117 111 70 18 NaeI GCC'GGC Cuts at: 3924 3924 Size: 7507 NarI GG'CG_CC Cuts at: 3920 3920 Size: 7507 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5998 Fragments arranged by size: 5998 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7507 NgoMIV G'CCGG_C Cuts at: 3922 3922 Size: 7507 NheI G'CTAG_C Cuts at: 1052 3950 1052 Size: 2898 4609 NotI GC'GGCC_GC Cuts at: 4954 4954 Size: 7507 NsiI A_TGCA'T Cuts at: 3156 3156 Size: 7507 NspI r_CATG'y Cuts at: 1132 1229 4865 6410 1132 Size: 97 3636 1545 2229 Fragments arranged by size: 3636 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3799 1348 Size: 2451 5056 PciI A'CATG_T Cuts at: 6406 6406 Size: 7507 PflMI CCAn_nnn'nTGG Cuts at: 1108 3233 1108 Size: 2125 5382 PfoI T'CCnGG_A Cuts at: 3939 4353 3939 Size: 414 7093 PmeI GTTT'AAAC Cuts at: 4811 4811 Size: 7507 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4155 4187 5576 5608 7089 7121 4155 Size: 32 1389 32 1481 32 4541 Fragments arranged by size: 4541 1481 1389 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3595 3943 3595 Size: 348 7159 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7507 PsiI TTA'TAA Cuts at: 5077 5077 Size: 7507 PstI C_TGCA'G Cuts at: 830 4065 4161 4215 4859 830 Size: 3235 96 54 644 3478 Fragments arranged by size: 3478 3235 644 96 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7507 PvuII CAG'CTG Cuts at: 2188 2614 3957 4027 4156 4273 2188 Size: 426 1343 70 129 117 5422 Fragments arranged by size: 5422 1343 426 129 117 70 RsrII CG'GwC_CG Cuts at: 2065 2065 Size: 7507 SacI G_AGCT'C Cuts at: 2665 3722 4250 2665 Size: 1057 528 5922 Fragments arranged by size: 5922 1057 528 SacII CC_GC'GG Cuts at: 4139 4139 Size: 7507 SalI G'TCGA_C Cuts at: 1936 4849 1936 Size: 2913 4594 SbfI CC_TGCA'GG Cuts at: 4859 4859 Size: 7507 ScaI AGT'ACT Cuts at: 5755 7467 5755 Size: 1712 5795 SfcI C'TryA_G Cuts at: 826 1045 4061 4157 4211 4475 4855 5990 6671 Size: 219 3016 96 54 264 380 1135 681 Cuts at: 6671 6862 826 Size: 191 1471 Fragments arranged by size: 3016 1471 1135 681 380 264 219 191 96 54 SfoI GGC'GCC Cuts at: 3921 3921 Size: 7507 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7507 SgrAI Cr'CCGG_yG Cuts at: 1700 3922 1700 Size: 2222 5285 SmaI CCC'GGG Cuts at: 1872 4448 4834 1872 Size: 2576 386 4545 Fragments arranged by size: 4545 2576 386 SmlI C'TyrA_G Cuts at: 820 1017 1943 2204 3626 4145 5611 6512 6774 Size: 197 926 261 1422 519 1466 901 262 Cuts at: 6774 820 Size: 1553 Fragments arranged by size: 1553 1466 1422 926 901 519 262 261 197 SnaBI TAC'GTA Cuts at: 493 4804 5298 493 Size: 4311 494 2702 Fragments arranged by size: 4311 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7507 SphI G_CATG'C Cuts at: 1132 4865 1132 Size: 3733 3774 SspI AAT'ATT Cuts at: 5 52 5431 5 Size: 47 5379 2081 Fragments arranged by size: 5379 2081 47 StuI AGG'CCT Cuts at: 1517 3215 4217 1517 Size: 1698 1002 4807 Fragments arranged by size: 4807 1698 1002 StyI C'CwwG_G Cuts at: 513 1065 2022 2941 3120 4946 513 Size: 552 957 919 179 1826 3074 Fragments arranged by size: 3074 1826 957 919 552 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2695 3812 5534 5551 5704 5889 6334 Size: 1255 1115 1117 1722 17 153 185 445 Cuts at: 6334 325 Size: 1498 Fragments arranged by size: 1722 1498 1255 1117 1115 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2224 5753 7465 Size: 274 80 33 51 1443 247 3529 1712 Cuts at: 7465 96 Size: 138 Fragments arranged by size: 3529 1712 1443 274 247 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3254 3286 4426 4458 7467 7499 3254 Size: 32 1140 32 3009 32 3262 Fragments arranged by size: 3262 3009 1140 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7507 XbaI T'CTAG_A Cuts at: 3856 4843 3856 Size: 987 6520 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2155 3361 2155 Size: 1206 6301 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7507 XmaI C'CCGG_G Cuts at: 1870 4446 4832 1870 Size: 2576 386 4545 Fragments arranged by size: 4545 2576 386 XmnI GAAnn'nnTTC Cuts at: 3524 5636 3524 Size: 2112 5395 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5315 276 Size: 53 83 186 936 3781 2468 Fragments arranged by size: 3781 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI SanDI SapI SexAI SfiI SrfI SwaI