Restriction map for
pFN21ASDA0781
(Linear) (Six-base) MAPSORT of: KIAA0781.seq Check: 8637 from: 1 to: 2778
KIAA0781.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 1722 1789 2155 2380 2778
Size: 371 1351 67 366 225 398
Fragments arranged by size:
1351 398 371 366 225 67
AfeI AGC'GCT
Cuts at: 0 2559 2778
Size: 2559 219
AflII C'TTAA_G
Cuts at: 0 1603 2778
Size: 1603 1175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1109 2778
Size: 1109 1669
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2132 2164 2778
Size: 2132 32 614
Fragments arranged by size:
2132 614 32
AlwNI CAG_nnn'CTG
Cuts at: 0 900 1210 1326 1692 1812 2022 2037 2133
Size: 900 310 116 366 120 210 15 96
Cuts at: 2133 2250 2319 2403 2509 2778
Size: 117 69 84 106 269
Fragments arranged by size:
900 366 310 269 210 120 117 116
106 96 84 69 15
ApaLI G'TGCA_C
Cuts at: 0 415 2778
Size: 415 2363
ApoI r'AATT_y
Cuts at: 0 308 364 492 1499 1829 2778
Size: 308 56 128 1007 330 949
Fragments arranged by size:
1007 949 330 308 128 56
AvaI C'yCGr_G
Cuts at: 0 18 2423 2778
Size: 18 2405 355
Fragments arranged by size:
2405 355 18
BamHI G'GATC_C
Cuts at: 0 2745 2778
Size: 2745 33
BanI G'GyrC_C
Cuts at: 0 113 1896 2778
Size: 113 1783 882
Fragments arranged by size:
1783 882 113
BanII G_rGCy'C
Cuts at: 0 642 1118 1699 2227 2326 2616 2778
Size: 642 476 581 528 99 290 162
Fragments arranged by size:
642 581 528 476 290 162 99
BbeI G_GCGC'C
Cuts at: 0 1900 2778
Size: 1900 878
BbsI GAAGACnn'nnnn_
Cuts at: 0 1397 1595 2778
Size: 1397 198 1183
Fragments arranged by size:
1397 1183 198
BbvCI CC'TCA_GC
Cuts at: 0 1910 2371 2778
Size: 1910 461 407
Fragments arranged by size:
1910 461 407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 9 43 2778
Size: 9 34 2735
Fragments arranged by size:
2735 34 9
BciVI GTATCCnnnnn_n'
Cuts at: 0 2609 2778
Size: 2609 169
BfrBI ATG'CAT
Cuts at: 0 1131 2778
Size: 1131 1647
BglI GCCn_nnn'nGGC
Cuts at: 0 1411 1687 2778
Size: 1411 276 1091
Fragments arranged by size:
1411 1091 276
BlpI GC'TnA_GC
Cuts at: 0 1082 2778
Size: 1082 1696
Bme1580I G_kGCm'C
Cuts at: 0 73 419 2778
Size: 73 346 2359
Fragments arranged by size:
2359 346 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 1027 1114 2025 2778
Size: 1027 87 911 753
Fragments arranged by size:
1027 911 753 87
BmtI G_CTAG'C
Cuts at: 0 1931 2778
Size: 1931 847
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2358 2691 2778
Size: 2358 333 87
Fragments arranged by size:
2358 333 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 1387 1712 1900 2035 2044 2134 2353
Size: 621 766 325 188 135 9 90 219
Cuts at: 2353 2661 2778
Size: 308 117
Fragments arranged by size:
766 621 325 308 219 188 135 117
90 9
Bpu10I CC'TnA_GC
Cuts at: 0 1910 2320 2371 2778
Size: 1910 410 51 407
Fragments arranged by size:
1910 410 407 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 2143 2778
Size: 202 1941 635
Fragments arranged by size:
1941 635 202
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 828 1339 1659 1730 2020 2778
Size: 253 575 511 320 71 290 758
Fragments arranged by size:
758 575 511 320 290 253 71
BsaHI Gr'CG_yC
Cuts at: 0 1897 2778
Size: 1897 881
BsaWI w'CCGG_w
Cuts at: 0 116 701 1875 2416 2778
Size: 116 585 1174 541 362
Fragments arranged by size:
1174 585 541 362 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1507 1537 2017 2047 2132 2162 2778
Size: 1507 30 480 30 85 30 616
Fragments arranged by size:
1507 616 480 85 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1156 1898 2423 2426 2778
Size: 1156 742 525 3 352
Fragments arranged by size:
1156 742 525 352 3
BseYI C'CCAG_C
Cuts at: 0 75 105 1330 1677 2000 2059 2312 2514
Size: 75 30 1225 347 323 59 253 202
Cuts at: 2514 2615 2778
Size: 101 163
Fragments arranged by size:
1225 347 323 253 202 163 101 75
59 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1870 2778
Size: 1870 908
BsiHKAI G_wGCw'C
Cuts at: 0 419 642 737 1699 2227 2778
Size: 419 223 95 962 528 551
Fragments arranged by size:
962 551 528 419 223 95
BsmI GAATG_Cn'
Cuts at: 0 1131 1231 2778
Size: 1131 100 1547
Fragments arranged by size:
1547 1131 100
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1434 1977 2778
Size: 1434 543 801
Fragments arranged by size:
1434 801 543
Bsp1286I G_dGCh'C
Cuts at: 0 73 419 642 737 1118 1699 2227 2326
Size: 73 346 223 95 381 581 528 99
Cuts at: 2326 2616 2778
Size: 290 162
Fragments arranged by size:
581 528 381 346 290 223 162 99
95 73
BspEI T'CCGG_A
Cuts at: 0 701 2778
Size: 701 2077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 885 2045 2778
Size: 885 1160 733
Fragments arranged by size:
1160 885 733
BsrBI CCG'CTC
Cuts at: 0 1399 2778
Size: 1399 1379
BsrDI GCAATG_nn'
Cuts at: 0 1076 2778
Size: 1076 1702
BsrFI r'CCGG_y
Cuts at: 0 39 335 1899 2778
Size: 39 296 1564 879
Fragments arranged by size:
1564 879 296 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 900 2133 2778
Size: 900 1233 645
Fragments arranged by size:
1233 900 645
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1473 2778
Size: 1473 1305
BstYI r'GATC_y
Cuts at: 0 2745 2778
Size: 2745 33
Bsu36I CC'TnA_GG
Cuts at: 0 1940 2778
Size: 1940 838
BtgI C'CryG_G
Cuts at: 0 2011 2113 2778
Size: 2011 102 665
Fragments arranged by size:
2011 665 102
BtsI GCAGTG_nn'
Cuts at: 0 179 1324 2778
Size: 179 1145 1454
Fragments arranged by size:
1454 1145 179
ClaI AT'CG_AT
Cuts at: 0 152 2778
Size: 152 2626
DraI TTT'AAA
Cuts at: 0 501 2778
Size: 501 2277
EaeI y'GGCC_r
Cuts at: 0 333 1053 2778
Size: 333 720 1725
Fragments arranged by size:
1725 720 333
EarI CTCTTCn'nnn_
Cuts at: 0 668 1238 2778
Size: 668 570 1540
Fragments arranged by size:
1540 668 570
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 1237 2778
Size: 25 1212 1541
Fragments arranged by size:
1541 1212 25
EcoICRI GAG'CTC
Cuts at: 0 640 1697 2225 2778
Size: 640 1057 528 553
Fragments arranged by size:
1057 640 553 528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 621 1387 1712 1722 1789 1900 2035
Size: 371 250 766 325 10 67 111 135
Cuts at: 2035 2044 2134 2155 2353 2380 2661 2778
Size: 9 90 21 198 27 281 117
Fragments arranged by size:
766 371 325 281 250 198 135 117
111 90 67 27 21 10 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1171 1490 1774 2778
Size: 1171 319 284 1004
Fragments arranged by size:
1171 1004 319 284
EcoO109I rG'GnC_Cy
Cuts at: 0 1572 1920 2292 2670 2778
Size: 1572 348 372 378 108
Fragments arranged by size:
1572 378 372 348 108
EcoRI G'AATT_C
Cuts at: 0 1829 2778
Size: 1829 949
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 105 2181 2579 2778
Size: 105 2076 398 199
Fragments arranged by size:
2076 398 199 105
HaeII r_GCGC'y
Cuts at: 0 1008 1900 2561 2778
Size: 1008 892 661 217
Fragments arranged by size:
1008 892 661 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 827 859 1148 1180 1507 1539 1543 1575
Size: 827 32 289 32 327 32 4 32
Cuts at: 1575 2778
Size: 1203
Fragments arranged by size:
1203 827 327 289 32 32 32 32
4
KasI G'GCGC_C
Cuts at: 0 1896 2778
Size: 1896 882
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1205 2778
Size: 385 820 1573
Fragments arranged by size:
1573 820 385
MspA1I CmG'CkG
Cuts at: 0 165 591 1433 1934 2004 2115 2133 2250
Size: 165 426 842 501 70 111 18 117
Cuts at: 2250 2778
Size: 528
Fragments arranged by size:
842 528 501 426 165 117 111 70
18
NaeI GCC'GGC
Cuts at: 0 1901 2778
Size: 1901 877
NarI GG'CG_CC
Cuts at: 0 1897 2778
Size: 1897 881
NgoMIV G'CCGG_C
Cuts at: 0 1899 2778
Size: 1899 879
NheI G'CTAG_C
Cuts at: 0 1927 2778
Size: 1927 851
NsiI A_TGCA'T
Cuts at: 0 1133 2778
Size: 1133 1645
PasI CC'CwG_GG
Cuts at: 0 1776 2778
Size: 1776 1002
PflMI CCAn_nnn'nTGG
Cuts at: 0 1210 2778
Size: 1210 1568
PfoI T'CCnGG_A
Cuts at: 0 1916 2330 2778
Size: 1916 414 448
Fragments arranged by size:
1916 448 414
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2132 2164 2778
Size: 2132 32 614
Fragments arranged by size:
2132 614 32
PpuMI rG'GwC_Cy
Cuts at: 0 1572 1920 2778
Size: 1572 348 858
Fragments arranged by size:
1572 858 348
PstI C_TGCA'G
Cuts at: 0 2042 2138 2192 2778
Size: 2042 96 54 586
Fragments arranged by size:
2042 586 96 54
PvuII CAG'CTG
Cuts at: 0 165 591 1934 2004 2133 2250 2778
Size: 165 426 1343 70 129 117 528
Fragments arranged by size:
1343 528 426 165 129 117 70
RsrII CG'GwC_CG
Cuts at: 0 42 2778
Size: 42 2736
SacI G_AGCT'C
Cuts at: 0 642 1699 2227 2778
Size: 642 1057 528 551
Fragments arranged by size:
1057 642 551 528
SacII CC_GC'GG
Cuts at: 0 2116 2778
Size: 2116 662
SfcI C'TryA_G
Cuts at: 0 2038 2134 2188 2452 2778
Size: 2038 96 54 264 326
Fragments arranged by size:
2038 326 264 96 54
SfoI GGC'GCC
Cuts at: 0 1898 2778
Size: 1898 880
SgrAI Cr'CCGG_yG
Cuts at: 0 1899 2778
Size: 1899 879
SmaI CCC'GGG
Cuts at: 0 2425 2778
Size: 2425 353
SmlI C'TyrA_G
Cuts at: 0 181 1603 2122 2778
Size: 181 1422 519 656
Fragments arranged by size:
1422 656 519 181
StuI AGG'CCT
Cuts at: 0 1192 2194 2778
Size: 1192 1002 584
Fragments arranged by size:
1192 1002 584
StyI C'CwwG_G
Cuts at: 0 918 1097 2778
Size: 918 179 1681
Fragments arranged by size:
1681 918 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 672 1789 2778
Size: 672 1117 989
Fragments arranged by size:
1117 989 672
TatI w'GTAC_w
Cuts at: 0 201 2778
Size: 201 2577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1231 1263 2403 2435 2778
Size: 1231 32 1140 32 343
Fragments arranged by size:
1231 1140 343 32 32
XbaI T'CTAG_A
Cuts at: 0 1833 2778
Size: 1833 945
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 132 1338 2778
Size: 132 1206 1440
Fragments arranged by size:
1440 1206 132
XmaI C'CCGG_G
Cuts at: 0 2423 2778
Size: 2423 355
XmnI GAAnn'nnTTC
Cuts at: 0 1501 2778
Size: 1501 1277
Enzymes that do cut:
AcuI AfeI AflII AlfI AloI AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BfrBI BglI BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
BtsI ClaI DraI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI MmeI
MspA1I NaeI NarI NgoMIV NheI NsiI PasI PflMI
PfoI PpiI PpuMI PstI PvuII RsrII SacI SacII
SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII
TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AleI ApaI AscI AseI AsiSI AvrII BaeI BclI
BglII BmgBI BsaAI BsaBI BsiEI BsiWI BspHI BsrGI
BssHII BssSI BstBI BstEII BstZ17I BtgZI CspCI DraIII
DrdI EagI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI MslI NcoI NdeI
NotI NruI NspI PacI PciI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XhoI ZraI