(Circular) (Six-base) MAPSORT of: pFN21ASDA0776.seq Check: 5662 from: 1 to: 7111 pFN21ASDA0776 7111 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3043 3043 Size: 7111 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4921 278 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3813 4454 1937 Size: 1876 641 4594 Fragments arranged by size: 4594 1876 641 Acc65I G'GTAC_C Cuts at: 4432 4432 Size: 7111 AclI AA'CG_TT Cuts at: 1563 5238 5611 1563 Size: 3675 373 3063 Fragments arranged by size: 3675 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3032 3234 3764 5174 6558 6826 1632 Size: 1400 202 530 1410 1384 268 1917 Fragments arranged by size: 1917 1410 1400 1384 530 268 202 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6914 AflIII A'CryG_T Cuts at: 1203 2233 6010 6987 1203 Size: 1030 3777 977 1327 Fragments arranged by size: 3777 1327 1030 977 AgeI A'CCGG_T Cuts at: 5942 5942 Size: 7111 AhdI GACnn_n'nnGTC Cuts at: 5840 5840 Size: 7111 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7111 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7111 AlwNI CAG_nnn'CTG Cuts at: 2861 4015 4854 6426 2861 Size: 1154 839 1572 3546 Fragments arranged by size: 3546 1572 1154 839 ApaLI G'TGCA_C Cuts at: 5168 6324 5168 Size: 1156 5955 ApoI r'AATT_y Cuts at: 1493 2632 2825 3122 3852 4179 4420 4646 4798 Size: 1139 193 297 730 327 241 226 152 Cuts at: 4798 6907 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1139 730 327 297 241 226 193 152 AseI AT'TA_AT Cuts at: 160 1032 2280 5665 160 Size: 872 1248 3385 1606 Fragments arranged by size: 3385 1606 1248 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 AvaI C'yCGr_G Cuts at: 1870 1943 3563 4436 1870 Size: 73 1620 873 4545 Fragments arranged by size: 4545 1620 873 73 BamHI G'GATC_C Cuts at: 4441 4808 4441 Size: 367 6744 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4432 5887 618 Size: 325 217 627 2645 1455 1842 Fragments arranged by size: 2645 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4430 1391 Size: 3039 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3582 3936 4068 4262 928 Size: 971 1683 354 132 194 3777 Fragments arranged by size: 3777 1683 971 354 194 132 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5302 5336 1903 Size: 34 3365 34 3678 Fragments arranged by size: 3678 3365 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2600 5003 6213 2600 Size: 2403 1210 3498 Fragments arranged by size: 3498 2403 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7111 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5722 136 Size: 107 122 71 631 605 4050 1525 Fragments arranged by size: 4050 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3220 7106 3220 Size: 3886 3225 BlpI GC'TnA_GC Cuts at: 4528 4528 Size: 7111 Bme1580I G_kGCm'C Cuts at: 1790 5172 6328 1790 Size: 3382 1156 2573 Fragments arranged by size: 3382 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7111 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4269 4842 5800 Size: 416 53 83 399 24 2852 573 958 Cuts at: 5800 442 Size: 1753 Fragments arranged by size: 2852 1753 958 573 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2181 2755 5771 1239 Size: 942 574 3016 2579 Fragments arranged by size: 3016 2579 942 574 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2705 5236 6101 6399 2705 Size: 2531 865 298 3417 Fragments arranged by size: 3417 2531 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5774 882 Size: 617 4275 2219 Fragments arranged by size: 4275 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2588 4408 4902 493 Size: 711 1384 1820 494 2702 Fragments arranged by size: 2702 1820 1384 711 494 BsaBI GATnn'nnATC Cuts at: 7105 7105 Size: 7111 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 4918 5300 5935 Size: 53 83 186 936 498 2887 382 635 Cuts at: 5935 275 Size: 1451 Fragments arranged by size: 2887 1451 936 635 498 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5544 5942 6216 6363 6774 5544 Size: 398 274 147 411 5881 Fragments arranged by size: 5881 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2753 2783 1178 Size: 30 165 30 1350 30 5506 Fragments arranged by size: 5506 1350 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2559 3336 3339 3521 1186 Size: 1373 777 3 182 4776 Fragments arranged by size: 4776 1373 777 182 3 BseYI C'CCAG_C Cuts at: 6314 6810 6314 Size: 496 6615 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4064 1163 Size: 573 2328 4210 Fragments arranged by size: 4210 2328 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 4561 5322 6350 1540 Size: 275 186 60 2500 761 1028 2301 Fragments arranged by size: 2500 2301 1028 761 275 186 60 BsiHKAI G_wGCw'C Cuts at: 5172 5257 6328 5172 Size: 85 1071 5955 Fragments arranged by size: 5955 1071 85 BsmI GAATG_Cn' Cuts at: 3065 4622 4715 3065 Size: 1557 93 5461 Fragments arranged by size: 5461 1557 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4430 5172 5257 6328 1391 Size: 399 2640 742 85 1071 2174 Fragments arranged by size: 2640 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6774 6774 Size: 7111 BspHI T'CATG_A Cuts at: 5000 6730 5000 Size: 1730 5381 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3043 4466 844 Size: 2199 1423 3489 Fragments arranged by size: 3489 2199 1423 BsrBI CCG'CTC Cuts at: 1394 1823 2110 4998 1394 Size: 429 287 2888 3507 Fragments arranged by size: 3507 2888 429 287 BsrDI GCAATG_nn' Cuts at: 65 1248 3343 4158 5606 5780 65 Size: 1183 2095 815 1448 174 1396 Fragments arranged by size: 2095 1448 1396 1183 815 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5755 5942 1700 Size: 110 3945 187 2869 Fragments arranged by size: 3945 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3137 3663 96 Size: 3041 526 3544 Fragments arranged by size: 3544 3041 526 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7111 BssSI C'ACGA_G Cuts at: 5171 6183 5171 Size: 1012 6099 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7111 BstBI TT'CG_AA Cuts at: 2007 5915 2007 Size: 3908 3203 BstEII G'GTnAC_C Cuts at: 1184 2431 1184 Size: 1247 5864 BstXI CCAn_nnnn'nTGG Cuts at: 4136 4136 Size: 7111 BstYI r'GATC_y Cuts at: 1972 3220 4441 4808 5193 5210 6662 6748 6760 Size: 1248 1221 367 385 17 1452 86 12 Cuts at: 6760 7106 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1248 1221 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3504 3504 Size: 7111 BtgI C'CryG_G Cuts at: 513 1065 2022 2593 513 Size: 552 957 571 5031 Fragments arranged by size: 5031 957 571 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6372 Fragments arranged by size: 6372 683 56 BtsI GCAGTG_nn' Cuts at: 3313 4627 5421 5441 3313 Size: 1314 794 20 4983 Fragments arranged by size: 4983 1314 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7111 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7076 DraI TTT'AAA Cuts at: 4415 4415 Size: 7111 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6118 809 Size: 507 4802 1802 Fragments arranged by size: 4802 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 4558 5447 Size: 54 1425 50 275 18 228 2500 889 Cuts at: 5447 7076 8 Size: 1629 43 Fragments arranged by size: 2500 1629 1425 889 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 4558 1537 Size: 275 246 2500 4090 Fragments arranged by size: 4090 2500 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 2034 2391 4395 5041 1353 Size: 681 357 2004 646 3423 Fragments arranged by size: 3423 2004 681 646 357 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 5698 6072 6218 387 Size: 1064 138 453 3656 374 146 1280 Fragments arranged by size: 3656 1280 1064 453 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2181 2755 3032 3234 3764 5174 5771 Size: 393 549 574 277 202 530 1410 597 Cuts at: 5771 6558 6826 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1410 787 597 574 549 530 393 277 268 202 EcoNI CCTnn'n_nnAGG Cuts at: 2803 2803 Size: 7111 EcoRI G'AATT_C Cuts at: 2632 4420 4798 2632 Size: 1788 378 4945 Fragments arranged by size: 4945 1788 378 EcoRV GAT'ATC Cuts at: 3763 3763 Size: 7111 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3905 3905 Size: 7111 FspI TGC'GCA Cuts at: 5617 5617 Size: 7111 HaeII r_GCGC'y Cuts at: 1678 6258 1678 Size: 4580 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2753 2785 2822 2835 2854 Size: 32 135 32 1181 32 37 13 19 Cuts at: 2854 2867 3389 3421 5752 5784 5826 5858 1373 Size: 13 522 32 2331 32 42 32 2626 Fragments arranged by size: 2626 2331 1181 522 135 42 37 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 4455 4701 669 Size: 988 281 2517 246 3079 Fragments arranged by size: 3079 2517 988 281 246 HindIII A'AGCT_T Cuts at: 748 2562 3963 748 Size: 1814 1401 3896 Fragments arranged by size: 3896 1814 1401 HpaI GTT'AAC Cuts at: 4701 4701 Size: 7111 KpnI G_GTAC'C Cuts at: 4436 4436 Size: 7111 MfeI C'AATT_G Cuts at: 4710 4710 Size: 7111 MluI A'CGCG_T Cuts at: 6987 6987 Size: 7111 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4045 6225 6409 1305 Size: 2740 2180 184 2007 Fragments arranged by size: 2740 2180 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5289 Fragments arranged by size: 5289 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5069 5428 5587 518 Size: 648 3903 359 159 2042 Fragments arranged by size: 3903 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2627 4527 5204 6352 6597 1606 Size: 465 556 1900 677 1148 245 2120 Fragments arranged by size: 2120 1900 1148 677 556 465 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5602 Fragments arranged by size: 5602 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7111 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7111 NotI GC'GGCC_GC Cuts at: 4558 4558 Size: 7111 NspI r_CATG'y Cuts at: 1132 1229 2237 2342 4126 4199 4469 6014 1132 Size: 97 1008 105 1784 73 270 1545 2229 Fragments arranged by size: 2229 1784 1545 1008 270 105 97 73 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7111 PciI A'CATG_T Cuts at: 2233 6010 2233 Size: 3777 3334 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7111 PfoI T'CCnGG_A Cuts at: 2771 3456 2771 Size: 685 6426 PmeI GTTT'AAAC Cuts at: 4415 4415 Size: 7111 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5180 5212 6693 6725 5180 Size: 32 1481 32 5566 Fragments arranged by size: 5566 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7111 PsiI TTA'TAA Cuts at: 3128 4681 3128 Size: 1553 5558 PstI C_TGCA'G Cuts at: 830 3826 3958 4463 830 Size: 2996 132 505 3478 Fragments arranged by size: 3478 2996 505 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7111 PvuII CAG'CTG Cuts at: 2627 2627 Size: 7111 SalI G'TCGA_C Cuts at: 1936 4453 1936 Size: 2517 4594 SbfI CC_TGCA'GG Cuts at: 4463 4463 Size: 7111 ScaI AGT'ACT Cuts at: 2785 3533 4321 5359 7071 2785 Size: 748 788 1038 1712 2825 Fragments arranged by size: 2825 1712 1038 788 748 SfcI C'TryA_G Cuts at: 826 1045 2622 3087 3822 3954 4459 5594 6275 Size: 219 1577 465 735 132 505 1135 681 Cuts at: 6275 6466 826 Size: 191 1471 Fragments arranged by size: 1577 1471 1135 735 681 505 465 219 191 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7111 SmaI CCC'GGG Cuts at: 1872 4438 1872 Size: 2566 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2684 3563 5215 6116 6378 820 Size: 197 926 741 879 1652 901 262 1553 Fragments arranged by size: 1652 1553 926 901 879 741 262 197 SnaBI TAC'GTA Cuts at: 493 4408 4902 493 Size: 3915 494 2702 Fragments arranged by size: 3915 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7111 SphI G_CATG'C Cuts at: 1132 4126 4199 4469 1132 Size: 2994 73 270 3774 Fragments arranged by size: 3774 2994 270 73 SspI AAT'ATT Cuts at: 5 52 5035 5 Size: 47 4983 2081 Fragments arranged by size: 4983 2081 47 StuI AGG'CCT Cuts at: 1517 3080 1517 Size: 1563 5548 StyI C'CwwG_G Cuts at: 513 1065 2022 4550 513 Size: 552 957 2528 3074 Fragments arranged by size: 3074 2528 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2779 5138 5155 5308 5493 5938 325 Size: 1255 1199 2359 17 153 185 445 1498 Fragments arranged by size: 2359 1498 1255 1199 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2783 3137 3254 Size: 274 80 33 51 1443 806 354 117 Cuts at: 3254 3348 3531 3663 3755 4319 5357 7069 96 Size: 94 183 132 92 564 1038 1712 138 Fragments arranged by size: 1712 1443 1038 806 564 354 274 183 138 132 117 94 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7071 7103 7071 Size: 32 7079 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7111 XbaI T'CTAG_A Cuts at: 2821 4447 2821 Size: 1626 5485 XhoI C'TCGA_G Cuts at: 1943 3563 1943 Size: 1620 5491 XmaI C'CCGG_G Cuts at: 1870 4436 1870 Size: 2566 4545 XmnI GAAnn'nnTTC Cuts at: 2163 5240 2163 Size: 3077 4034 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4919 276 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI