Restriction map for
pFN21ASDA0776
(Linear) (Six-base) MAPSORT of: KIAA0776.seq Check: 765 from: 1 to: 2382
KIAA0776.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1020 2382
Size: 1020 1362
AccI GT'mk_AC
Cuts at: 0 1790 2382
Size: 1790 592
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 1211 1741 2382
Size: 1009 202 530 641
Fragments arranged by size:
1009 641 530 202
AflIII A'CryG_T
Cuts at: 0 210 2382
Size: 210 2172
AlwNI CAG_nnn'CTG
Cuts at: 0 838 1992 2382
Size: 838 1154 390
Fragments arranged by size:
1154 838 390
ApoI r'AATT_y
Cuts at: 0 609 802 1099 1829 2156 2382
Size: 609 193 297 730 327 226
Fragments arranged by size:
730 609 327 297 226 193
AseI AT'TA_AT
Cuts at: 0 257 2382
Size: 257 2125
AvaI C'yCGr_G
Cuts at: 0 1540 2382
Size: 1540 842
BbsI GAAGACnn'nnnn_
Cuts at: 0 1559 1913 2045 2239 2382
Size: 1559 354 132 194 143
Fragments arranged by size:
1559 354 194 143 132
BciVI GTATCCnnnnn_n'
Cuts at: 0 577 2382
Size: 577 1805
BglII A'GATC_T
Cuts at: 0 1197 2382
Size: 1197 1185
BmrI ACTGGGnnnn_n'
Cuts at: 0 2246 2382
Size: 2246 136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 732 2382
Size: 158 574 1650
Fragments arranged by size:
1650 574 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 2382
Size: 682 1700
BsaAI yAC'GTr
Cuts at: 0 565 2382
Size: 565 1817
BsaHI Gr'CG_yC
Cuts at: 0 8 2382
Size: 8 2374
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 2382
Size: 730 30 1622
Fragments arranged by size:
1622 730 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 536 1313 1316 1498 2382
Size: 536 777 3 182 884
Fragments arranged by size:
884 777 536 182 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2041 2382
Size: 2041 341
BsiEI CG_ry'CG
Cuts at: 0 38 2382
Size: 38 2344
BsmI GAATG_Cn'
Cuts at: 0 1042 2382
Size: 1042 1340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1020 2382
Size: 1020 1362
BsrBI CCG'CTC
Cuts at: 0 87 2382
Size: 87 2295
BsrDI GCAATG_nn'
Cuts at: 0 1320 2135 2382
Size: 1320 815 247
Fragments arranged by size:
1320 815 247
BsrGI T'GTAC_A
Cuts at: 0 1114 1640 2382
Size: 1114 526 742
Fragments arranged by size:
1114 742 526
BstEII G'GTnAC_C
Cuts at: 0 408 2382
Size: 408 1974
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2113 2382
Size: 2113 269
BstYI r'GATC_y
Cuts at: 0 1197 2382
Size: 1197 1185
Bsu36I CC'TnA_GG
Cuts at: 0 1481 2382
Size: 1481 901
BtgI C'CryG_G
Cuts at: 0 570 2382
Size: 570 1812
BtsI GCAGTG_nn'
Cuts at: 0 1290 2382
Size: 1290 1092
EaeI y'GGCC_r
Cuts at: 0 35 2382
Size: 35 2347
EagI C'GGCC_G
Cuts at: 0 35 2382
Size: 35 2347
EarI CTCTTCn'nnn_
Cuts at: 0 11 368 2372 2382
Size: 11 357 2004 10
Fragments arranged by size:
2004 357 11 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2382
Size: 19 2363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 732 1009 1211 1741 2382
Size: 158 574 277 202 530 641
Fragments arranged by size:
641 574 530 277 202 158
EcoNI CCTnn'n_nnAGG
Cuts at: 0 780 2382
Size: 780 1602
EcoRI G'AATT_C
Cuts at: 0 609 2382
Size: 609 1773
EcoRV GAT'ATC
Cuts at: 0 1740 2382
Size: 1740 642
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1882 2382
Size: 1882 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 730 762 799 812 831 844 1366 1398
Size: 730 32 37 13 19 13 522 32
Cuts at: 1398 2382
Size: 984
Fragments arranged by size:
984 730 522 37 32 32 19 13
13
HindIII A'AGCT_T
Cuts at: 0 539 1940 2382
Size: 539 1401 442
Fragments arranged by size:
1401 539 442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2022 2382
Size: 2022 360
MspA1I CmG'CkG
Cuts at: 0 48 604 2382
Size: 48 556 1778
Fragments arranged by size:
1778 556 48
NspI r_CATG'y
Cuts at: 0 214 319 2103 2176 2382
Size: 214 105 1784 73 206
Fragments arranged by size:
1784 214 206 105 73
PciI A'CATG_T
Cuts at: 0 210 2382
Size: 210 2172
PfoI T'CCnGG_A
Cuts at: 0 748 1433 2382
Size: 748 685 949
Fragments arranged by size:
949 748 685
PsiI TTA'TAA
Cuts at: 0 1105 2382
Size: 1105 1277
PstI C_TGCA'G
Cuts at: 0 1803 1935 2382
Size: 1803 132 447
Fragments arranged by size:
1803 447 132
PvuII CAG'CTG
Cuts at: 0 604 2382
Size: 604 1778
ScaI AGT'ACT
Cuts at: 0 762 1510 2298 2382
Size: 762 748 788 84
Fragments arranged by size:
788 762 748 84
SfcI C'TryA_G
Cuts at: 0 599 1064 1799 1931 2382
Size: 599 465 735 132 451
Fragments arranged by size:
735 599 465 451 132
SmlI C'TyrA_G
Cuts at: 0 661 1540 2382
Size: 661 879 842
Fragments arranged by size:
879 842 661
SphI G_CATG'C
Cuts at: 0 2103 2176 2382
Size: 2103 73 206
Fragments arranged by size:
2103 206 73
StuI AGG'CCT
Cuts at: 0 1057 2382
Size: 1057 1325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 756 2382
Size: 756 1626
TatI w'GTAC_w
Cuts at: 0 760 1114 1231 1325 1508 1640 1732 2296
Size: 760 354 117 94 183 132 92 564
Cuts at: 2296 2382
Size: 86
Fragments arranged by size:
760 564 354 183 132 117 94 92
86
XbaI T'CTAG_A
Cuts at: 0 798 2382
Size: 798 1584
XhoI C'TCGA_G
Cuts at: 0 1540 2382
Size: 1540 842
XmnI GAAnn'nnTTC
Cuts at: 0 140 2382
Size: 140 2242
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApoI AseI AvaI
BbsI BciVI BglII BmrI BpmI BpuEI BsaAI BsaHI
BsaXI BseRI BsgI BsiEI BsmI BspMI BsrBI BsrDI
BsrGI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI
Hin4I HindIII MmeI MspA1I NspI PciI PfoI PsiI
PstI PvuII ScaI SfcI SmlI SphI StuI TaqII
TatI XbaI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaI
BsaBI BsaWI BseYI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BsrFI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoO109I FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StyI SwaI TstI Tth111I XcmI XmaI ZraI