(Circular) (Six-base) MAPSORT of: pFN21ASDA0775.seq Check: 7890 from: 1 to: 6574 pFN21ASDA0775 6574 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2505 2505 Size: 6574 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4384 278 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2235 3917 1937 Size: 298 1682 4594 Fragments arranged by size: 4594 1682 298 Acc65I G'GTAC_C Cuts at: 3810 3895 3810 Size: 85 6489 AclI AA'CG_TT Cuts at: 1563 4701 5074 1563 Size: 3138 373 3063 Fragments arranged by size: 3138 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2825 4637 6021 6289 1632 Size: 1193 1812 1384 268 1917 Fragments arranged by size: 1917 1812 1384 1193 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 2127 5473 6450 1203 Size: 924 3346 977 1327 Fragments arranged by size: 3346 1327 977 924 AgeI A'CCGG_T Cuts at: 5405 5405 Size: 6574 AhdI GACnn_n'nnGTC Cuts at: 5303 5303 Size: 6574 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6574 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3578 1184 Size: 2394 4180 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2018 2050 2018 Size: 32 6542 AlwNI CAG_nnn'CTG Cuts at: 2527 2836 4317 5889 2527 Size: 309 1481 1572 3212 Fragments arranged by size: 3212 1572 1481 309 ApaI G_GGCC'C Cuts at: 2482 2830 2482 Size: 348 6226 ApaLI G'TGCA_C Cuts at: 4631 5787 4631 Size: 1156 5418 ApoI r'AATT_y Cuts at: 1493 2899 3883 4109 4261 6370 1493 Size: 1406 984 226 152 2109 1697 Fragments arranged by size: 2109 1697 1406 984 226 152 AscI GG'CGCG_CC Cuts at: 3541 3541 Size: 6574 AseI AT'TA_AT Cuts at: 160 1032 5128 160 Size: 872 4096 1606 Fragments arranged by size: 4096 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 2742 2823 3084 3489 3677 3899 1870 Size: 73 799 81 261 405 188 222 4545 Fragments arranged by size: 4545 799 405 261 222 188 81 73 BamHI G'GATC_C Cuts at: 3904 4271 3904 Size: 367 6207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3226 3271 3440 3635 3810 Size: 325 217 627 1439 45 169 195 175 Cuts at: 3810 3895 5350 618 Size: 85 1455 1842 Fragments arranged by size: 1842 1455 1439 627 325 217 195 175 169 85 45 BanII G_rGCy'C Cuts at: 1391 2482 2576 2672 2752 2830 3510 3764 3893 Size: 1091 94 96 80 78 680 254 129 Cuts at: 3893 1391 Size: 4072 Fragments arranged by size: 4072 1091 680 254 129 96 94 80 78 BbeI G_GCGC'C Cuts at: 3444 3444 Size: 6574 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5603 BbvCI CC'TCA_GC Cuts at: 2465 3530 2465 Size: 1065 5509 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4765 4799 1903 Size: 34 2828 34 3678 Fragments arranged by size: 3678 2828 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4466 5676 4466 Size: 1210 5364 BclI T'GATC_A Cuts at: 1548 2256 2890 1548 Size: 708 634 5232 Fragments arranged by size: 5232 708 634 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2747 3089 3470 Size: 107 122 71 631 605 1075 342 381 Cuts at: 3470 5185 136 Size: 1715 1525 Fragments arranged by size: 1715 1525 1075 631 605 381 342 122 107 71 BglII A'GATC_T Cuts at: 2505 3864 6569 2505 Size: 1359 2705 2510 Fragments arranged by size: 2705 2510 1359 BlpI GC'TnA_GC Cuts at: 2751 3991 2751 Size: 1240 5334 Bme1580I G_kGCm'C Cuts at: 1790 2482 2830 3065 3104 3147 3155 3276 3609 Size: 692 348 235 39 43 8 121 333 Cuts at: 3609 3638 4635 5791 1790 Size: 29 997 1156 2573 Fragments arranged by size: 2573 1156 997 692 348 333 235 121 43 39 29 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6574 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2673 3052 4305 Size: 416 53 83 399 24 1256 379 1253 Cuts at: 4305 5263 442 Size: 958 1753 Fragments arranged by size: 1753 1256 1253 958 416 399 379 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2339 2531 2714 2756 3872 5234 1239 Size: 977 123 192 183 42 1116 1362 2579 Fragments arranged by size: 2579 1362 1116 977 192 183 123 42 Bpu10I CC'TnA_GC Cuts at: 2465 2570 3530 2465 Size: 105 960 5509 Fragments arranged by size: 5509 960 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2668 2998 4699 5564 5862 2668 Size: 330 1701 865 298 3380 Fragments arranged by size: 3380 1701 865 330 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2100 2813 5237 882 Size: 617 601 713 2424 2219 Fragments arranged by size: 2424 2219 713 617 601 BsaAI yAC'GTr Cuts at: 493 1204 3376 3871 4365 493 Size: 711 2172 495 494 2702 Fragments arranged by size: 2702 2172 711 495 494 BsaBI GATnn'nnATC Cuts at: 6568 6568 Size: 6574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2221 3441 4381 4763 Size: 53 83 186 936 688 1220 940 382 Cuts at: 4763 5398 275 Size: 635 1451 Fragments arranged by size: 1451 1220 940 936 688 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5007 5405 5679 5826 6237 5007 Size: 398 274 147 411 5344 Fragments arranged by size: 5344 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2018 2048 2117 2147 3581 Size: 30 165 30 615 30 69 30 1434 Cuts at: 3581 3611 3827 3857 1178 Size: 30 216 30 3895 Fragments arranged by size: 3895 1434 615 216 165 69 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2777 2945 3602 1186 Size: 1102 489 168 657 4158 Fragments arranged by size: 4158 1102 657 489 168 BseYI C'CCAG_C Cuts at: 2185 2935 3126 3248 3278 3365 3620 3793 5777 Size: 750 191 122 30 87 255 173 1984 Cuts at: 5777 6273 2185 Size: 496 2486 Fragments arranged by size: 2486 1984 750 496 255 191 173 122 87 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2584 2600 1163 Size: 573 848 16 5137 Fragments arranged by size: 5137 848 573 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4024 4785 5813 1540 Size: 275 186 2023 761 1028 2301 Fragments arranged by size: 2301 2023 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2135 3510 3717 4635 4720 5791 2135 Size: 1375 207 918 85 1071 2918 Fragments arranged by size: 2918 1375 1071 918 207 85 BsmI GAATG_Cn' Cuts at: 4085 4178 4085 Size: 93 6481 BsmBI CGTCTCn'nnnn_ Cuts at: 2483 3746 2483 Size: 1263 5311 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2482 2576 2672 2752 2830 3065 Size: 399 345 347 94 96 80 78 235 Cuts at: 3065 3104 3147 3155 3276 3510 3609 3638 3717 Size: 39 43 8 121 234 99 29 79 Cuts at: 3717 3764 3893 4635 4720 5791 1391 Size: 47 129 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 347 345 235 234 129 121 99 96 94 85 80 79 78 47 43 39 29 8 BspEI T'CCGG_A Cuts at: 6237 6237 Size: 6574 BspHI T'CATG_A Cuts at: 4463 6193 4463 Size: 1730 4844 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2505 2622 3929 844 Size: 1661 117 1307 3489 Fragments arranged by size: 3489 1661 1307 117 BsrBI CCG'CTC Cuts at: 1394 1823 2698 2788 3089 3135 3174 3219 4461 Size: 429 875 90 301 46 39 45 1242 Cuts at: 4461 1394 Size: 3507 Fragments arranged by size: 3507 1242 875 429 301 90 46 45 39 BsrDI GCAATG_nn' Cuts at: 65 1248 5069 5243 65 Size: 1183 3821 174 1396 Fragments arranged by size: 3821 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2940 3223 3383 5218 5405 1700 Size: 110 1130 283 160 1835 187 2869 Fragments arranged by size: 2869 1835 1130 283 187 160 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6574 BssHII G'CGCG_C Cuts at: 1925 3346 3541 1925 Size: 1421 195 4958 Fragments arranged by size: 4958 1421 195 BssSI C'ACGA_G Cuts at: 4634 5646 4634 Size: 1012 5562 BstAPI GCAn_nnn'nTGC Cuts at: 794 2527 794 Size: 1733 4841 BstBI TT'CG_AA Cuts at: 2007 5378 2007 Size: 3371 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6574 BstYI r'GATC_y Cuts at: 1972 2505 2639 2833 3864 3904 4271 4656 4673 Size: 533 134 194 1031 40 367 385 17 Cuts at: 4673 6125 6211 6223 6569 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1031 533 385 367 346 194 134 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3536 513 Size: 552 957 1514 3551 Fragments arranged by size: 3551 1514 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5835 Fragments arranged by size: 5835 683 56 BtsI GCAGTG_nn' Cuts at: 2157 4090 4884 4904 2157 Size: 1933 794 20 3827 Fragments arranged by size: 3827 1933 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 3878 3878 Size: 6574 DraIII CAC_nnn'GTG Cuts at: 2681 3376 2681 Size: 695 5879 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5581 809 Size: 507 4265 1802 Fragments arranged by size: 4265 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2663 3013 3302 Size: 54 1425 50 275 18 833 350 289 Cuts at: 3302 3339 3471 3728 4021 4910 6539 8 Size: 37 132 257 293 889 1629 43 Fragments arranged by size: 1629 1425 889 833 350 293 289 275 257 132 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 4021 1537 Size: 275 2209 4090 Fragments arranged by size: 4090 2209 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2438 2706 4504 1353 Size: 1085 268 1798 3423 Fragments arranged by size: 3423 1798 1085 268 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2125 3434 5161 5535 5681 387 Size: 1064 138 536 1309 1727 374 146 1280 Fragments arranged by size: 1727 1309 1280 1064 536 374 146 138 EcoICRI GAG'CTC Cuts at: 3508 3508 Size: 6574 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2339 2531 2714 2756 2825 3872 Size: 393 584 123 192 183 42 69 1047 Cuts at: 3872 4637 5234 6021 6289 1239 Size: 765 597 787 268 1524 Fragments arranged by size: 1524 1047 787 765 597 584 393 268 192 183 123 69 42 EcoNI CCTnn'n_nnAGG Cuts at: 2060 2318 2060 Size: 258 6316 EcoO109I rG'GnC_Cy Cuts at: 2068 2075 2191 2478 2479 3525 2068 Size: 7 116 287 1 1046 5117 Fragments arranged by size: 5117 1046 287 116 7 1 EcoRI G'AATT_C Cuts at: 3883 4261 3883 Size: 378 6196 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2417 2467 3403 2417 Size: 50 936 5588 Fragments arranged by size: 5588 936 50 FspI TGC'GCA Cuts at: 5080 5080 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 2909 3133 3172 3217 3262 3444 5721 1678 Size: 1231 224 39 45 45 182 2277 2531 Fragments arranged by size: 2531 2277 1231 224 182 45 45 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 2211 2243 2397 Size: 32 135 32 545 32 62 32 154 Cuts at: 2397 2429 2825 2857 5215 5247 5289 5321 1373 Size: 32 396 32 2358 32 42 32 2626 Fragments arranged by size: 2626 2358 545 396 154 135 62 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3918 4164 669 Size: 988 281 1980 246 3079 Fragments arranged by size: 3079 1980 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6574 HpaI GTT'AAC Cuts at: 4164 4164 Size: 6574 KasI G'GCGC_C Cuts at: 3440 3440 Size: 6574 KpnI G_GTAC'C Cuts at: 3814 3899 3814 Size: 85 6489 MfeI C'AATT_G Cuts at: 4173 4173 Size: 6574 MluI A'CGCG_T Cuts at: 6450 6450 Size: 6574 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2242 2589 2832 2868 3564 3789 5688 5872 Size: 937 347 243 36 696 225 1899 184 Cuts at: 5872 1305 Size: 2007 Fragments arranged by size: 2007 1899 937 696 347 243 225 184 36 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4752 Fragments arranged by size: 4752 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4532 4891 5050 518 Size: 648 3366 359 159 2042 Fragments arranged by size: 3366 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2527 2812 3031 3282 3538 3645 3777 3793 Size: 921 285 219 251 256 107 132 16 Cuts at: 3793 3990 4667 5815 6060 1606 Size: 197 677 1148 245 2120 Fragments arranged by size: 2120 1148 921 677 285 256 251 245 219 197 132 107 16 NaeI GCC'GGC Cuts at: 2942 3385 2942 Size: 443 6131 NarI GG'CG_CC Cuts at: 3441 3441 Size: 6574 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5065 Fragments arranged by size: 5065 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6574 NgoMIV G'CCGG_C Cuts at: 2940 3383 2940 Size: 443 6131 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 2131 3932 5477 1132 Size: 97 902 1801 1545 2229 Fragments arranged by size: 2229 1801 1545 902 97 PasI CC'CwG_GG Cuts at: 1348 2071 1348 Size: 723 5851 PciI A'CATG_T Cuts at: 2127 5473 2127 Size: 3346 3228 PflMI CCAn_nnn'nTGG Cuts at: 1108 2836 1108 Size: 1728 4846 PfoI T'CCnGG_A Cuts at: 2101 2547 2757 2101 Size: 446 210 5918 Fragments arranged by size: 5918 446 210 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PmlI CAC'GTG Cuts at: 3376 3376 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2018 2050 4643 4675 6156 6188 2018 Size: 32 2593 32 1481 32 2404 Fragments arranged by size: 2593 2404 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 4144 4144 Size: 6574 PspOMI G'GGCC_C Cuts at: 2478 2826 2478 Size: 348 6226 PstI C_TGCA'G Cuts at: 830 2343 2796 2817 3462 3926 830 Size: 1513 453 21 645 464 3478 Fragments arranged by size: 3478 1513 645 464 453 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 2527 2812 3282 3777 3793 2527 Size: 285 470 495 16 5308 Fragments arranged by size: 5308 495 470 285 16 SacI G_AGCT'C Cuts at: 3510 3510 Size: 6574 SacII CC_GC'GG Cuts at: 3539 3539 Size: 6574 SalI G'TCGA_C Cuts at: 1936 3916 1936 Size: 1980 4594 SbfI CC_TGCA'GG Cuts at: 3926 3926 Size: 6574 ScaI AGT'ACT Cuts at: 4822 6534 4822 Size: 1712 4862 SfcI C'TryA_G Cuts at: 826 1045 2339 2792 2813 2963 3458 3922 5057 Size: 219 1294 453 21 150 495 464 1135 Cuts at: 5057 5738 5929 826 Size: 681 191 1471 Fragments arranged by size: 1471 1294 1135 681 495 464 453 219 191 150 21 SfiI GGCCn_nnn'nGGCC Cuts at: 3470 3470 Size: 6574 SfoI GGC'GCC Cuts at: 3442 3442 Size: 6574 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 3223 1700 Size: 1523 5051 SmaI CCC'GGG Cuts at: 1872 2744 2825 3491 3901 1872 Size: 872 81 666 410 4545 Fragments arranged by size: 4545 872 666 410 81 SmlI C'TyrA_G Cuts at: 820 1017 1943 2647 2977 4678 5579 5841 820 Size: 197 926 704 330 1701 901 262 1553 Fragments arranged by size: 1701 1553 926 901 704 330 262 197 SnaBI TAC'GTA Cuts at: 493 3871 4365 493 Size: 3378 494 2702 Fragments arranged by size: 3378 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 3932 1132 Size: 2800 3774 SspI AAT'ATT Cuts at: 5 52 4498 5 Size: 47 4446 2081 Fragments arranged by size: 4446 2081 47 StuI AGG'CCT Cuts at: 1517 2420 2544 1517 Size: 903 124 5547 Fragments arranged by size: 5547 903 124 StyI C'CwwG_G Cuts at: 513 1065 2022 4013 513 Size: 552 957 1991 3074 Fragments arranged by size: 3074 1991 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2692 2860 4601 4618 4771 4956 5401 Size: 1255 1112 168 1741 17 153 185 445 Cuts at: 5401 325 Size: 1498 Fragments arranged by size: 1741 1498 1255 1112 445 185 168 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4820 6532 96 Size: 274 80 33 51 1443 2843 1712 138 Fragments arranged by size: 2843 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3581 3613 3707 3739 3827 3859 6534 6566 3581 Size: 32 94 32 88 32 2675 32 3589 Fragments arranged by size: 3589 2675 94 88 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6574 XbaI T'CTAG_A Cuts at: 3910 3910 Size: 6574 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3628 3628 Size: 6574 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6574 XmaI C'CCGG_G Cuts at: 1870 2742 2823 3489 3899 1870 Size: 872 81 666 410 4545 Fragments arranged by size: 4545 872 666 410 81 XmnI GAAnn'nnTTC Cuts at: 4703 4703 Size: 6574 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4382 276 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI