(Circular) (Six-base) MAPSORT of: pFN21ASDA0770.seq Check: 1665 from: 1 to: 7354 pFN21ASDA0770 7354 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3538 4501 3538 Size: 963 6391 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5164 278 Size: 53 83 186 936 3628 2468 Fragments arranged by size: 3628 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4697 1937 Size: 2760 4594 Acc65I G'GTAC_C Cuts at: 4675 4675 Size: 7354 AclI AA'CG_TT Cuts at: 1563 5481 5854 1563 Size: 3918 373 3063 Fragments arranged by size: 3918 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3719 3732 5417 6801 7069 1632 Size: 2087 13 1685 1384 268 1917 Fragments arranged by size: 2087 1917 1685 1384 268 13 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7157 AflIII A'CryG_T Cuts at: 1203 6253 7230 1203 Size: 5050 977 1327 Fragments arranged by size: 5050 1327 977 AgeI A'CCGG_T Cuts at: 2375 6185 2375 Size: 3810 3544 AhdI GACnn_n'nnGTC Cuts at: 6083 6083 Size: 7354 AleI CACnn'nnGTG Cuts at: 1166 3122 1166 Size: 1956 5398 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2846 3111 1184 Size: 1662 265 5427 Fragments arranged by size: 5427 1662 265 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3795 3827 4053 4085 3795 Size: 32 226 32 7064 Fragments arranged by size: 7064 226 32 32 AlwNI CAG_nnn'CTG Cuts at: 3616 3625 5097 6669 3616 Size: 9 1472 1572 4301 Fragments arranged by size: 4301 1572 1472 9 ApaLI G'TGCA_C Cuts at: 5411 6567 5411 Size: 1156 6198 ApoI r'AATT_y Cuts at: 1493 2447 2512 3021 3772 4472 4663 4889 5041 Size: 954 65 509 751 700 191 226 152 Cuts at: 5041 7150 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 954 751 700 509 226 191 152 65 AseI AT'TA_AT Cuts at: 160 1032 5908 160 Size: 872 4876 1606 Fragments arranged by size: 4876 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7354 AvaI C'yCGr_G Cuts at: 1870 1943 4313 4679 1870 Size: 73 2370 366 4545 Fragments arranged by size: 4545 2370 366 73 BamHI G'GATC_C Cuts at: 4684 5051 4684 Size: 367 6987 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4675 6130 618 Size: 325 217 627 2888 1455 1842 Fragments arranged by size: 2888 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2586 2613 3531 4069 4673 1391 Size: 1195 27 918 538 604 4072 Fragments arranged by size: 4072 1195 918 604 538 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6383 BbvCI CC'TCA_GC Cuts at: 4146 4146 Size: 7354 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5545 5579 1903 Size: 34 3608 34 3678 Fragments arranged by size: 3678 3608 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3189 4126 5246 6456 3189 Size: 937 1120 1210 4087 Fragments arranged by size: 4087 1210 1120 937 BclI T'GATC_A Cuts at: 1548 3292 1548 Size: 1744 5610 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3416 5965 136 Size: 107 122 71 631 605 1744 2549 1525 Fragments arranged by size: 2549 1744 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3715 7349 3715 Size: 3634 3720 BlpI GC'TnA_GC Cuts at: 4771 4771 Size: 7354 Bme1580I G_kGCm'C Cuts at: 1790 2490 2710 3632 5415 6571 1790 Size: 700 220 922 1783 1156 2573 Fragments arranged by size: 2573 1783 1156 922 700 220 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7354 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2236 3923 5085 Size: 416 53 83 399 24 819 1687 1162 Cuts at: 5085 6043 442 Size: 958 1753 Fragments arranged by size: 1753 1687 1162 958 819 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7354 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3850 3850 Size: 7354 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2627 2930 3455 3960 4277 4295 6014 Size: 1106 282 303 525 505 317 18 1719 Cuts at: 6014 1239 Size: 2579 Fragments arranged by size: 2579 1719 1106 525 505 317 303 282 18 Bpu10I CC'TnA_GC Cuts at: 4146 4146 Size: 7354 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3368 4436 5479 6344 6642 3368 Size: 1068 1043 865 298 4080 Fragments arranged by size: 4080 1068 1043 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2366 6017 882 Size: 617 867 3651 2219 Fragments arranged by size: 3651 2219 867 617 BsaAI yAC'GTr Cuts at: 493 1204 3472 3743 4651 5145 493 Size: 711 2268 271 908 494 2702 Fragments arranged by size: 2702 2268 908 711 494 271 BsaBI GATnn'nnATC Cuts at: 4200 7348 4200 Size: 3148 4206 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5161 5543 6178 275 Size: 53 83 186 936 3628 382 635 1451 Fragments arranged by size: 3628 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2375 5787 6185 6459 6606 7017 2375 Size: 3412 398 274 147 411 2712 Fragments arranged by size: 3412 2712 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2928 2958 4053 4083 4526 Size: 30 165 30 1525 30 1095 30 443 Cuts at: 4526 4556 4590 4620 1178 Size: 30 34 30 3912 Fragments arranged by size: 3912 1525 1095 443 165 34 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 3473 3839 3971 4163 4547 1186 Size: 1210 1077 366 132 192 384 3993 Fragments arranged by size: 3993 1210 1077 384 366 192 132 BseYI C'CCAG_C Cuts at: 3934 3958 4235 4636 6557 7053 3934 Size: 24 277 401 1921 496 4235 Fragments arranged by size: 4235 1921 496 401 277 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2677 3629 1163 Size: 573 941 952 4888 Fragments arranged by size: 4888 952 941 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4804 5565 6593 1540 Size: 275 186 2803 761 1028 2301 Fragments arranged by size: 2803 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2586 2745 3201 3471 3555 4069 4452 5415 5500 Size: 159 456 270 84 514 383 963 85 Cuts at: 5500 6571 2586 Size: 1071 3369 Fragments arranged by size: 3369 1071 963 514 456 383 270 159 85 84 BsmI GAATG_Cn' Cuts at: 4239 4865 4958 4239 Size: 626 93 6635 Fragments arranged by size: 6635 626 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2490 2586 2613 2710 2745 3201 3471 Size: 399 700 96 27 97 35 456 270 Cuts at: 3471 3531 3555 3632 4069 4452 4673 5415 5500 Size: 60 24 77 437 383 221 742 85 Cuts at: 5500 6571 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 700 456 437 399 383 270 221 97 96 85 77 60 35 27 24 BspEI T'CCGG_A Cuts at: 7017 7017 Size: 7354 BspHI T'CATG_A Cuts at: 2452 5243 6973 2452 Size: 2791 1730 2833 Fragments arranged by size: 2833 2791 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3079 3153 3538 3648 4297 4501 4709 844 Size: 2235 74 385 110 649 204 208 3489 Fragments arranged by size: 3489 2235 649 385 208 204 110 74 BsrBI CCG'CTC Cuts at: 1394 1823 3316 4301 4495 5241 1394 Size: 429 1493 985 194 746 3507 Fragments arranged by size: 3507 1493 985 746 429 194 BsrDI GCAATG_nn' Cuts at: 65 1248 2760 3188 5849 6023 65 Size: 1183 1512 428 2661 174 1396 Fragments arranged by size: 2661 1512 1396 1183 428 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2375 3969 4020 5998 6185 1700 Size: 110 565 1594 51 1978 187 2869 Fragments arranged by size: 2869 1978 1594 565 187 110 51 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7354 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7354 BssSI C'ACGA_G Cuts at: 3533 3554 3596 5414 6426 3533 Size: 21 42 1818 1012 4461 Fragments arranged by size: 4461 1818 1012 42 21 BstAPI GCAn_nnn'nTGC Cuts at: 794 3616 4245 794 Size: 2822 629 3903 Fragments arranged by size: 3903 2822 629 BstBI TT'CG_AA Cuts at: 2007 6158 2007 Size: 4151 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7354 BstXI CCAn_nnnn'nTGG Cuts at: 2497 4600 2497 Size: 2103 5251 BstYI r'GATC_y Cuts at: 1972 2226 2688 2787 3112 3715 4684 5051 5436 Size: 254 462 99 325 603 969 367 385 Cuts at: 5436 5453 6905 6991 7003 7349 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 969 603 462 385 367 346 325 254 99 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2854 4478 2854 Size: 1624 5730 BtgI C'CryG_G Cuts at: 513 1065 2496 2667 2736 2919 513 Size: 552 1431 171 69 183 4948 Fragments arranged by size: 4948 1431 552 183 171 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6615 Fragments arranged by size: 6615 683 56 BtsI GCAGTG_nn' Cuts at: 2772 3445 4243 4870 5664 5684 2772 Size: 673 798 627 794 20 4442 Fragments arranged by size: 4442 798 794 673 627 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7354 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7319 DraI TTT'AAA Cuts at: 4658 4658 Size: 7354 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6361 809 Size: 507 5045 1802 Fragments arranged by size: 5045 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2733 2880 4801 Size: 54 1425 50 275 18 903 147 1921 Cuts at: 4801 5690 7319 8 Size: 889 1629 43 Fragments arranged by size: 1921 1629 1425 903 889 275 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4801 1537 Size: 275 2989 4090 Fragments arranged by size: 4090 2989 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3062 3942 4485 5284 1353 Size: 1709 880 543 799 3423 Fragments arranged by size: 3423 1709 880 799 543 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5941 6315 6461 387 Size: 1064 138 4352 374 146 1280 Fragments arranged by size: 4352 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2584 4067 2584 Size: 1483 5871 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2627 2930 3455 3719 3732 3960 Size: 393 713 282 303 525 264 13 228 Cuts at: 3960 4277 4295 5417 6014 6801 7069 1239 Size: 317 18 1122 597 787 268 1524 Fragments arranged by size: 1524 1122 787 713 597 525 393 317 303 282 268 264 228 18 13 EcoNI CCTnn'n_nnAGG Cuts at: 3588 4403 3588 Size: 815 6539 EcoO109I rG'GnC_Cy Cuts at: 2838 4309 4348 2838 Size: 1471 39 5844 Fragments arranged by size: 5844 1471 39 EcoRI G'AATT_C Cuts at: 4472 4663 5041 4472 Size: 191 378 6785 Fragments arranged by size: 6785 378 191 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2147 3244 2147 Size: 1097 6257 FspI TGC'GCA Cuts at: 5860 5860 Size: 7354 HaeII r_GCGC'y Cuts at: 1678 6501 1678 Size: 4823 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3284 3316 3820 3852 4590 Size: 32 135 32 1712 32 504 32 738 Cuts at: 4590 4622 5995 6027 6069 6101 1373 Size: 32 1373 32 42 32 2626 Fragments arranged by size: 2626 1712 1373 738 504 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4698 4944 669 Size: 988 281 2760 246 3079 Fragments arranged by size: 3079 2760 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7354 HpaI GTT'AAC Cuts at: 4944 4944 Size: 7354 KpnI G_GTAC'C Cuts at: 4679 4679 Size: 7354 MfeI C'AATT_G Cuts at: 3266 4953 3266 Size: 1687 5667 MluI A'CGCG_T Cuts at: 7230 7230 Size: 7354 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2615 2959 6468 6652 1305 Size: 1310 344 3509 184 2007 Fragments arranged by size: 3509 2007 1310 344 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2735 2882 10 Size: 54 1425 343 903 147 4482 Fragments arranged by size: 4482 1425 903 343 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2701 2729 2765 3122 3402 5312 5671 Size: 648 1535 28 36 357 280 1910 359 Cuts at: 5671 5830 518 Size: 159 2042 Fragments arranged by size: 2042 1910 1535 648 359 357 280 159 36 28 MspA1I CmG'CkG Cuts at: 1606 2605 3938 4640 4770 5447 6595 6840 1606 Size: 999 1333 702 130 677 1148 245 2120 Fragments arranged by size: 2120 1333 1148 999 702 677 245 130 NaeI GCC'GGC Cuts at: 4022 4022 Size: 7354 NcoI C'CATG_G Cuts at: 513 1065 2496 2736 2919 513 Size: 552 1431 240 183 4948 Fragments arranged by size: 4948 1431 552 240 183 NdeI CA'TA_TG Cuts at: 387 387 Size: 7354 NgoMIV G'CCGG_C Cuts at: 4020 4020 Size: 7354 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7354 NotI GC'GGCC_GC Cuts at: 4801 4801 Size: 7354 NspI r_CATG'y Cuts at: 1132 1229 4712 6257 1132 Size: 97 3483 1545 2229 Fragments arranged by size: 3483 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7354 PciI A'CATG_T Cuts at: 6253 6253 Size: 7354 PflMI CCAn_nnn'nTGG Cuts at: 1108 2502 3127 3692 1108 Size: 1394 625 565 4770 Fragments arranged by size: 4770 1394 625 565 PfoI T'CCnGG_A Cuts at: 2946 3720 4398 4485 2946 Size: 774 678 87 5815 Fragments arranged by size: 5815 774 678 87 PmeI GTTT'AAAC Cuts at: 4658 4658 Size: 7354 PmlI CAC'GTG Cuts at: 3472 3743 3472 Size: 271 7083 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4053 4085 5423 5455 6936 6968 4053 Size: 32 1338 32 1481 32 4439 Fragments arranged by size: 4439 1481 1338 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4309 4309 Size: 7354 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7354 PsiI TTA'TAA Cuts at: 4924 4924 Size: 7354 PspXI vC'TCGA_Gb Cuts at: 4313 4313 Size: 7354 PstI C_TGCA'G Cuts at: 830 2424 2976 3549 3621 3919 4308 4706 830 Size: 1594 552 573 72 298 389 398 3478 Fragments arranged by size: 3478 1594 573 552 398 389 298 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7354 PvuII CAG'CTG Cuts at: 2605 3938 4640 2605 Size: 1333 702 5319 Fragments arranged by size: 5319 1333 702 SacI G_AGCT'C Cuts at: 2586 4069 2586 Size: 1483 5871 SalI G'TCGA_C Cuts at: 1936 4696 1936 Size: 2760 4594 SapI GCTCTTCn'nnn_ Cuts at: 4485 4485 Size: 7354 SbfI CC_TGCA'GG Cuts at: 3919 4706 3919 Size: 787 6567 ScaI AGT'ACT Cuts at: 4154 5602 7314 4154 Size: 1448 1712 4194 Fragments arranged by size: 4194 1712 1448 SfcI C'TryA_G Cuts at: 826 1045 2140 2420 2972 3160 3545 3617 3915 Size: 219 1095 280 552 188 385 72 298 Cuts at: 3915 4304 4702 5837 6518 6709 826 Size: 389 398 1135 681 191 1471 Fragments arranged by size: 1471 1135 1095 681 552 398 389 385 298 280 219 191 188 72 SfiI GGCCn_nnn'nGGCC Cuts at: 3416 3416 Size: 7354 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7354 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7354 SmaI CCC'GGG Cuts at: 1872 4681 1872 Size: 2809 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3383 4313 4451 5458 6359 6621 Size: 197 926 1440 930 138 1007 901 262 Cuts at: 6621 820 Size: 1553 Fragments arranged by size: 1553 1440 1007 930 926 901 262 197 138 SnaBI TAC'GTA Cuts at: 493 4651 5145 493 Size: 4158 494 2702 Fragments arranged by size: 4158 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7354 SphI G_CATG'C Cuts at: 1132 4712 1132 Size: 3580 3774 SspI AAT'ATT Cuts at: 5 52 5278 5 Size: 47 5226 2081 Fragments arranged by size: 5226 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7354 StyI C'CwwG_G Cuts at: 513 1065 2496 2736 2919 4344 4793 513 Size: 552 1431 240 183 1425 449 3074 Fragments arranged by size: 3074 1431 1425 552 449 240 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3134 3658 4188 5381 5398 5551 5736 Size: 1255 1554 524 530 1193 17 153 185 Cuts at: 5736 6181 325 Size: 445 1498 Fragments arranged by size: 1554 1498 1255 1193 530 524 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3492 4152 5600 Size: 274 80 33 51 1443 1515 660 1448 Cuts at: 5600 7312 96 Size: 1712 138 Fragments arranged by size: 1712 1515 1448 1443 660 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4526 4558 7314 7346 4526 Size: 32 2756 32 4534 Fragments arranged by size: 4534 2756 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7354 XbaI T'CTAG_A Cuts at: 4690 4690 Size: 7354 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3124 3124 Size: 7354 XhoI C'TCGA_G Cuts at: 1943 4313 1943 Size: 2370 4984 XmaI C'CCGG_G Cuts at: 1870 4679 1870 Size: 2809 4545 XmnI GAAnn'nnTTC Cuts at: 2103 4436 5483 2103 Size: 2333 1047 3974 Fragments arranged by size: 3974 2333 1047 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5162 276 Size: 53 83 186 936 3628 2468 Fragments arranged by size: 3628 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI