(Circular) (Six-base) MAPSORT of: pFN21ASDA0769.seq Check: 9137 from: 1 to: 6781 pFN21ASDA0769 6781 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3281 3281 Size: 6781 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4591 278 Size: 53 83 186 936 3055 2468 Fragments arranged by size: 3055 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4124 1937 Size: 2187 4594 Acc65I G'GTAC_C Cuts at: 4102 4102 Size: 6781 AclI AA'CG_TT Cuts at: 1563 4908 5281 1563 Size: 3345 373 3063 Fragments arranged by size: 3345 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 2593 2687 3039 3885 4844 6228 6496 Size: 437 524 94 352 846 959 1384 268 Cuts at: 6496 1632 Size: 1917 Fragments arranged by size: 1917 1384 959 846 524 437 352 268 94 AflII C'TTAA_G Cuts at: 820 1017 3253 820 Size: 197 2236 4348 Fragments arranged by size: 4348 2236 197 AflIII A'CryG_T Cuts at: 1203 2852 2854 3498 5680 6657 1203 Size: 1649 2 644 2182 977 1327 Fragments arranged by size: 2182 1649 1327 977 644 2 AgeI A'CCGG_T Cuts at: 5612 5612 Size: 6781 AhdI GACnn_n'nnGTC Cuts at: 3490 5510 3490 Size: 2020 4761 AleI CACnn'nnGTG Cuts at: 1166 2855 4070 1166 Size: 1689 1215 3877 Fragments arranged by size: 3877 1689 1215 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6781 AlwNI CAG_nnn'CTG Cuts at: 2801 3472 4524 6096 2801 Size: 671 1052 1572 3486 Fragments arranged by size: 3486 1572 1052 671 ApaI G_GGCC'C Cuts at: 3124 3124 Size: 6781 ApaLI G'TGCA_C Cuts at: 4838 5994 4838 Size: 1156 5625 ApoI r'AATT_y Cuts at: 1493 3686 4090 4316 4468 6577 1493 Size: 2193 404 226 152 2109 1697 Fragments arranged by size: 2193 2109 1697 404 226 152 AseI AT'TA_AT Cuts at: 160 1032 5335 160 Size: 872 4303 1606 Fragments arranged by size: 4303 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6781 AvaI C'yCGr_G Cuts at: 1870 1943 2126 4106 1870 Size: 73 183 1980 4545 Fragments arranged by size: 4545 1980 183 73 BamHI G'GATC_C Cuts at: 4111 4478 4111 Size: 367 6414 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3248 4102 5557 618 Size: 325 217 627 1461 854 1455 1842 Fragments arranged by size: 1842 1461 1455 854 627 325 217 BanII G_rGCy'C Cuts at: 1391 3124 3878 4100 1391 Size: 1733 754 222 4072 Fragments arranged by size: 4072 1733 754 222 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3375 928 Size: 971 1476 4334 Fragments arranged by size: 4334 1476 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4972 5006 1903 Size: 34 3035 34 3678 Fragments arranged by size: 3678 3035 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2115 4673 5883 2115 Size: 2558 1210 3013 Fragments arranged by size: 3013 2558 1210 BclI T'GATC_A Cuts at: 1548 2581 1548 Size: 1033 5748 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3246 5392 136 Size: 107 122 71 631 605 1574 2146 1525 Fragments arranged by size: 2146 1574 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3780 6776 3780 Size: 2996 3785 BlpI GC'TnA_GC Cuts at: 4198 4198 Size: 6781 Bme1580I G_kGCm'C Cuts at: 1790 3124 4842 5998 1790 Size: 1334 1718 1156 2573 Fragments arranged by size: 2573 1718 1334 1156 BmgBI CAC'GTC Cuts at: 1318 3501 1318 Size: 2183 4598 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2143 2811 3382 Size: 416 53 83 399 24 726 668 571 Cuts at: 3382 4512 5470 442 Size: 1130 958 1753 Fragments arranged by size: 1753 1130 958 726 668 571 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6781 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2085 2659 2930 5441 1239 Size: 846 574 271 2511 2579 Fragments arranged by size: 2579 2511 846 574 271 Bpu10I CC'TnA_GC Cuts at: 3455 3455 Size: 6781 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2573 4906 5771 6069 2573 Size: 2333 865 298 3285 Fragments arranged by size: 3285 2333 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3900 3962 5444 882 Size: 617 2401 62 1482 2219 Fragments arranged by size: 2401 2219 1482 617 62 BsaAI yAC'GTr Cuts at: 493 1204 2855 2864 4078 4572 493 Size: 711 1651 9 1214 494 2702 Fragments arranged by size: 2702 1651 1214 711 494 9 BsaBI GATnn'nnATC Cuts at: 3779 6775 3779 Size: 2996 3785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4588 4970 5605 275 Size: 53 83 186 936 3055 382 635 1451 Fragments arranged by size: 3055 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5214 5612 5886 6033 6444 5214 Size: 398 274 147 411 5551 Fragments arranged by size: 5551 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2913 2943 1178 Size: 30 165 30 1510 30 5016 Fragments arranged by size: 5016 1510 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2828 3886 1186 Size: 1642 1058 4081 Fragments arranged by size: 4081 1642 1058 BseYI C'CCAG_C Cuts at: 2742 3842 3857 5984 6480 2742 Size: 1100 15 2127 496 3043 Fragments arranged by size: 3043 2127 1100 496 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2663 1163 Size: 573 927 5281 Fragments arranged by size: 5281 927 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4231 4992 6020 1540 Size: 275 186 2230 761 1028 2301 Fragments arranged by size: 2301 2230 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2820 3878 4842 4927 5998 2820 Size: 1058 964 85 1071 3603 Fragments arranged by size: 3603 1071 1058 964 85 BsmI GAATG_Cn' Cuts at: 4292 4385 4292 Size: 93 6688 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2820 3124 3878 4100 4842 4927 5998 Size: 399 1030 304 754 222 742 85 1071 Cuts at: 5998 1391 Size: 2174 Fragments arranged by size: 2174 1071 1030 754 742 399 304 222 85 BspEI T'CCGG_A Cuts at: 6444 6444 Size: 6781 BspHI T'CATG_A Cuts at: 4670 6400 4670 Size: 1730 5051 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2131 3042 3281 4136 844 Size: 1287 911 239 855 3489 Fragments arranged by size: 3489 1287 911 855 239 BsrBI CCG'CTC Cuts at: 1394 1823 4668 1394 Size: 429 2845 3507 Fragments arranged by size: 3507 2845 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5276 5450 65 Size: 1183 4028 174 1396 Fragments arranged by size: 4028 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5425 5612 1700 Size: 110 3615 187 2869 Fragments arranged by size: 3615 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6781 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6781 BssSI C'ACGA_G Cuts at: 4841 5853 4841 Size: 1012 5769 BstAPI GCAn_nnn'nTGC Cuts at: 794 2510 794 Size: 1716 5065 BstBI TT'CG_AA Cuts at: 2007 5585 2007 Size: 3578 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6781 BstXI CCAn_nnnn'nTGG Cuts at: 2683 2857 2683 Size: 174 6607 BstYI r'GATC_y Cuts at: 1972 2262 3780 4111 4478 4863 4880 6332 6418 Size: 290 1518 331 367 385 17 1452 86 Cuts at: 6418 6430 6776 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1518 1452 385 367 346 331 290 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3621 3621 Size: 6781 BtgI C'CryG_G Cuts at: 513 1065 3513 3761 513 Size: 552 2448 248 3533 Fragments arranged by size: 3533 2448 552 248 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3130 487 Size: 683 56 1904 4138 Fragments arranged by size: 4138 1904 683 56 BtsI GCAGTG_nn' Cuts at: 3141 3818 4297 5091 5111 3141 Size: 677 479 794 20 4811 Fragments arranged by size: 4811 794 677 479 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6781 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3807 3842 529 Size: 35 3243 35 3468 Fragments arranged by size: 3468 3243 35 35 DraI TTT'AAA Cuts at: 4085 4085 Size: 6781 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5788 809 Size: 507 4472 1802 Fragments arranged by size: 4472 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3397 3474 4228 Size: 54 1425 50 275 18 1567 77 754 Cuts at: 4228 5117 6746 8 Size: 889 1629 43 Fragments arranged by size: 1629 1567 1425 889 754 275 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4228 1537 Size: 275 2416 4090 Fragments arranged by size: 4090 2416 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 3189 4711 1353 Size: 692 1144 1522 3423 Fragments arranged by size: 3423 1522 1144 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5368 5742 5888 387 Size: 1064 138 3779 374 146 1280 Fragments arranged by size: 3779 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3876 3876 Size: 6781 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2085 2593 2659 2687 2930 3039 Size: 393 437 16 508 66 28 243 109 Cuts at: 3039 3885 4844 5441 6228 6496 1239 Size: 846 959 597 787 268 1524 Fragments arranged by size: 1524 959 846 787 597 508 437 393 268 243 109 66 28 16 EcoRI G'AATT_C Cuts at: 3686 4090 4468 3686 Size: 404 378 5999 Fragments arranged by size: 5999 404 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2335 2335 Size: 6781 FspI TGC'GCA Cuts at: 5287 5287 Size: 6781 HaeII r_GCGC'y Cuts at: 1678 5928 1678 Size: 4250 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2089 2121 2360 2392 3476 Size: 32 135 32 517 32 239 32 1084 Cuts at: 3476 3508 3596 3618 3628 3650 5422 5454 5496 Size: 32 88 22 10 22 1772 32 42 Cuts at: 5496 5528 1373 Size: 32 2626 Fragments arranged by size: 2626 1772 1084 517 239 135 88 42 32 32 32 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 2256 2548 3318 4125 4371 669 Size: 988 281 318 292 770 807 246 3079 Fragments arranged by size: 3079 988 807 770 318 292 281 246 HindIII A'AGCT_T Cuts at: 748 2215 748 Size: 1467 5314 HpaI GTT'AAC Cuts at: 2548 4371 2548 Size: 1823 4958 KpnI G_GTAC'C Cuts at: 4106 4106 Size: 6781 MfeI C'AATT_G Cuts at: 4380 4380 Size: 6781 MluI A'CGCG_T Cuts at: 6657 6657 Size: 6781 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3009 5895 6079 1305 Size: 1704 2886 184 2007 Fragments arranged by size: 2886 2007 1704 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3399 10 Size: 54 1425 343 1567 3392 Fragments arranged by size: 3392 1567 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2855 4070 4739 5098 5257 518 Size: 648 1689 1215 669 359 159 2042 Fragments arranged by size: 2042 1689 1215 669 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2707 4197 4874 6022 6267 1606 Size: 1101 1490 677 1148 245 2120 Fragments arranged by size: 2120 1490 1148 1101 677 245 NcoI C'CATG_G Cuts at: 513 1065 3761 513 Size: 552 2696 3533 Fragments arranged by size: 3533 2696 552 NdeI CA'TA_TG Cuts at: 387 3976 387 Size: 3589 3192 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6781 NotI GC'GGCC_GC Cuts at: 4228 4228 Size: 6781 NspI r_CATG'y Cuts at: 1132 1229 2104 2634 3463 4139 5684 1132 Size: 97 875 530 829 676 1545 2229 Fragments arranged by size: 2229 1545 875 829 676 530 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6781 PciI A'CATG_T Cuts at: 5680 5680 Size: 6781 PflMI CCAn_nnn'nTGG Cuts at: 1108 3094 1108 Size: 1986 4795 PfoI T'CCnGG_A Cuts at: 2688 2688 Size: 6781 PmeI GTTT'AAAC Cuts at: 4085 4085 Size: 6781 PmlI CAC'GTG Cuts at: 2855 2864 2855 Size: 9 6772 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4850 4882 6363 6395 4850 Size: 32 1481 32 5236 Fragments arranged by size: 5236 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6781 PsiI TTA'TAA Cuts at: 4351 4351 Size: 6781 PspOMI G'GGCC_C Cuts at: 3120 3120 Size: 6781 PstI C_TGCA'G Cuts at: 830 3136 3468 4133 830 Size: 2306 332 665 3478 Fragments arranged by size: 3478 2306 665 332 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6781 PvuII CAG'CTG Cuts at: 2707 2707 Size: 6781 SacI G_AGCT'C Cuts at: 3878 3878 Size: 6781 SalI G'TCGA_C Cuts at: 1936 4123 1936 Size: 2187 4594 SbfI CC_TGCA'GG Cuts at: 4133 4133 Size: 6781 ScaI AGT'ACT Cuts at: 5029 6741 5029 Size: 1712 5069 SfcI C'TryA_G Cuts at: 826 1045 3132 3428 3464 4129 5264 5945 6136 Size: 219 2087 296 36 665 1135 681 191 Cuts at: 6136 826 Size: 1471 Fragments arranged by size: 2087 1471 1135 681 665 296 219 191 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6781 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6781 SmaI CCC'GGG Cuts at: 1872 4108 1872 Size: 2236 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2126 2588 3253 4885 5786 6048 Size: 197 926 183 462 665 1632 901 262 Cuts at: 6048 820 Size: 1553 Fragments arranged by size: 1632 1553 926 901 665 462 262 197 183 SnaBI TAC'GTA Cuts at: 493 4078 4572 493 Size: 3585 494 2702 Fragments arranged by size: 3585 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6781 SphI G_CATG'C Cuts at: 1132 3463 4139 1132 Size: 2331 676 3774 Fragments arranged by size: 3774 2331 676 SspI AAT'ATT Cuts at: 5 52 2986 4705 5 Size: 47 2934 1719 2081 Fragments arranged by size: 2934 2081 1719 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6781 StyI C'CwwG_G Cuts at: 513 1065 3761 4220 513 Size: 552 2696 459 3074 Fragments arranged by size: 3074 2696 552 459 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3480 4808 4825 4978 5163 5608 325 Size: 1255 1900 1328 17 153 185 445 1498 Fragments arranged by size: 1900 1498 1328 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3882 5027 6739 Size: 274 80 33 51 1443 1905 1145 1712 Cuts at: 6739 96 Size: 138 Fragments arranged by size: 1905 1712 1443 1145 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6741 6773 6741 Size: 32 6749 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6781 XbaI T'CTAG_A Cuts at: 4117 4117 Size: 6781 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 3665 2029 Size: 1636 5145 XhoI C'TCGA_G Cuts at: 1943 2126 1943 Size: 183 6598 XmaI C'CCGG_G Cuts at: 1870 4106 1870 Size: 2236 4545 XmnI GAAnn'nnTTC Cuts at: 2651 2985 4910 2651 Size: 334 1925 4522 Fragments arranged by size: 4522 1925 334 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4589 276 Size: 53 83 186 936 3055 2468 Fragments arranged by size: 3055 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI