(Circular) (Six-base) MAPSORT of: pFN21ASDA0764.seq Check: 5817 from: 1 to: 5971 pFN21ASDA0764 5971 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2248 3781 278 Size: 53 83 186 936 712 1533 2468 Fragments arranged by size: 2468 1533 936 712 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2331 2805 4034 5418 5686 1632 Size: 699 474 1229 1384 268 1917 Fragments arranged by size: 1917 1384 1229 699 474 268 AfeI AGC'GCT Cuts at: 3239 3239 Size: 5971 AflII C'TTAA_G Cuts at: 820 1017 2594 820 Size: 197 1577 4197 Fragments arranged by size: 4197 1577 197 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 2926 3714 5286 2926 Size: 788 1572 3611 Fragments arranged by size: 3611 1572 788 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 3280 3506 3658 5767 1493 Size: 1787 226 152 2109 1697 Fragments arranged by size: 2109 1787 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 3296 1870 Size: 73 1353 4545 Fragments arranged by size: 4545 1353 73 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3292 4747 618 Size: 325 217 627 1505 1455 1842 Fragments arranged by size: 1842 1505 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2950 3164 3290 1391 Size: 1559 214 126 4072 Fragments arranged by size: 4072 1559 214 126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2240 3106 928 Size: 971 341 866 3793 Fragments arranged by size: 3793 971 866 341 BbvCI CC'TCA_GC Cuts at: 2149 2149 Size: 5971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2465 2499 4162 4196 1903 Size: 34 528 34 1663 34 3678 Fragments arranged by size: 3678 1663 528 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 2339 4032 5188 1790 Size: 549 1693 1156 2573 Fragments arranged by size: 2573 1693 1156 549 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2050 3702 4660 Size: 416 53 83 399 24 633 1652 958 Cuts at: 4660 442 Size: 1753 Fragments arranged by size: 1753 1652 958 633 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2340 2340 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2579 2699 2915 2934 2996 4631 1239 Size: 1340 120 216 19 62 1635 2579 Fragments arranged by size: 2579 1635 1340 216 120 62 19 Bpu10I CC'TnA_GC Cuts at: 2149 2271 2149 Size: 122 5849 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3045 4096 4961 5259 3045 Size: 1051 865 298 3757 Fragments arranged by size: 3757 1051 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2555 2910 4634 882 Size: 617 1056 355 1724 2219 Fragments arranged by size: 2219 1724 1056 617 355 BsaAI yAC'GTr Cuts at: 493 1204 3268 3762 493 Size: 711 2064 494 2702 Fragments arranged by size: 2702 2064 711 494 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2245 3778 4160 4795 Size: 53 83 186 936 712 1533 382 635 Cuts at: 4795 275 Size: 1451 Fragments arranged by size: 1533 1451 936 712 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2337 2367 3113 3143 1178 Size: 30 165 30 934 30 746 30 4006 Fragments arranged by size: 4006 934 746 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2340 2483 2903 3015 3134 1186 Size: 1154 143 420 112 119 4023 Fragments arranged by size: 4023 1154 420 143 119 112 BseYI C'CCAG_C Cuts at: 2641 3100 3146 5174 5670 2641 Size: 459 46 2028 496 2942 Fragments arranged by size: 2942 2028 496 459 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 3027 1163 Size: 573 387 904 4107 Fragments arranged by size: 4107 904 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2709 4032 4117 5188 2709 Size: 1323 85 1071 3492 Fragments arranged by size: 3492 1323 1071 85 BsmI GAATG_Cn' Cuts at: 3482 3575 3482 Size: 93 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2339 2709 2950 3164 3290 4032 4117 Size: 399 549 370 241 214 126 742 85 Cuts at: 4117 5188 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 549 399 370 241 214 126 85 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2665 3326 844 Size: 1821 661 3489 Fragments arranged by size: 3489 1821 661 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3085 4466 4640 65 Size: 1183 1837 1381 174 1396 Fragments arranged by size: 1837 1396 1381 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4615 4802 1700 Size: 110 2805 187 2869 Fragments arranged by size: 2869 2805 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 2332 4031 5043 2332 Size: 1699 1012 3260 Fragments arranged by size: 3260 1699 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3097 794 Size: 2303 3668 BstBI TT'CG_AA Cuts at: 2007 4775 2007 Size: 2768 3203 BstEII G'GTnAC_C Cuts at: 1184 2343 1184 Size: 1159 4812 BstXI CCAn_nnnn'nTGG Cuts at: 2179 2518 2179 Size: 339 5632 BstYI r'GATC_y Cuts at: 1972 2406 3301 3668 4053 4070 5522 5608 5620 Size: 434 895 367 385 17 1452 86 12 Cuts at: 5620 5966 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 895 434 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2099 513 Size: 552 1034 4385 Fragments arranged by size: 4385 1034 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 2511 2707 2924 3112 3487 4281 4301 2511 Size: 196 217 188 375 794 20 4181 Fragments arranged by size: 4181 794 375 217 196 188 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2508 3418 4307 Size: 54 1425 50 275 18 678 910 889 Cuts at: 4307 5936 8 Size: 1629 43 Fragments arranged by size: 1629 1425 910 889 678 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2307 2331 3242 3901 1353 Size: 954 24 911 659 3423 Fragments arranged by size: 3423 954 911 659 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2331 2579 2699 2805 2915 2934 2996 Size: 393 699 248 120 106 110 19 62 Cuts at: 2996 4034 4631 5418 5686 1239 Size: 1038 597 787 268 1524 Fragments arranged by size: 1524 1038 787 699 597 393 268 248 120 110 106 62 19 EcoNI CCTnn'n_nnAGG Cuts at: 2498 3129 2498 Size: 631 5340 EcoO109I rG'GnC_Cy Cuts at: 3216 3216 Size: 5971 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 3241 5118 1678 Size: 1563 1877 2531 Fragments arranged by size: 2531 1877 1563 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2174 2206 2791 2823 3113 Size: 32 135 32 602 32 585 32 290 Cuts at: 3113 3145 4612 4644 4686 4718 1373 Size: 32 1467 32 42 32 2626 Fragments arranged by size: 2626 1467 602 585 290 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 2985 748 Size: 2237 3734 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5085 5269 1305 Size: 3780 184 2007 Fragments arranged by size: 3780 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2510 10 Size: 54 1425 343 678 3471 Fragments arranged by size: 3471 1425 678 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3929 4288 4447 518 Size: 648 2763 359 159 2042 Fragments arranged by size: 2763 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2472 3387 4064 5212 5457 1606 Size: 866 915 677 1148 245 2120 Fragments arranged by size: 2120 1148 915 866 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5419 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 2761 3329 4874 1132 Size: 97 1532 568 1545 2229 Fragments arranged by size: 2229 1545 1532 568 97 PasI CC'CwG_GG Cuts at: 1348 2339 1348 Size: 991 4980 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 2506 2689 1108 Size: 1398 183 4390 Fragments arranged by size: 4390 1398 183 PfoI T'CCnGG_A Cuts at: 2556 2556 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3216 3216 Size: 5971 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PstI C_TGCA'G Cuts at: 830 3323 830 Size: 2493 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2472 2472 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 2762 3319 4454 5135 5326 826 Size: 219 1717 557 1135 681 191 1471 Fragments arranged by size: 1717 1471 1135 681 557 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2594 3060 4075 4976 5238 820 Size: 197 926 651 466 1015 901 262 1553 Fragments arranged by size: 1553 1015 926 901 651 466 262 197 SnaBI TAC'GTA Cuts at: 493 3268 3762 493 Size: 2775 494 2702 Fragments arranged by size: 2775 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5971 StyI C'CwwG_G Cuts at: 513 1065 3410 513 Size: 552 2345 3074 Fragments arranged by size: 3074 2345 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3998 4015 4168 4353 4798 325 Size: 1255 2418 17 153 185 445 1498 Fragments arranged by size: 2418 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4217 5929 96 Size: 274 80 33 51 1443 2240 1712 138 Fragments arranged by size: 2240 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2091 2123 2337 2369 5931 5963 2091 Size: 32 214 32 3562 32 2099 Fragments arranged by size: 3562 2099 214 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 2325 4100 2325 Size: 1775 4196 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2246 3779 276 Size: 53 83 186 936 712 1533 2468 Fragments arranged by size: 2468 1533 936 712 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI