(Circular) (Six-base) MAPSORT of: pFN21ASDA0760.seq Check: 2974 from: 1 to: 8401 pFN21ASDA0760 8401 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2054 3981 4129 4326 4695 5251 2054 Size: 1927 148 197 369 556 5204 Fragments arranged by size: 5204 1927 556 369 197 148 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5094 6211 278 Size: 53 83 186 936 3558 1117 2468 Fragments arranged by size: 3558 2468 1117 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2757 2856 3908 5744 1937 Size: 820 99 1052 1836 4594 Fragments arranged by size: 4594 1836 1052 820 99 Acc65I G'GTAC_C Cuts at: 4375 5722 4375 Size: 1347 7054 AclI AA'CG_TT Cuts at: 1563 4641 6528 6901 1563 Size: 3078 1887 373 3063 Fragments arranged by size: 3078 3063 1887 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2501 2641 3149 3815 4195 6464 7848 8116 Size: 869 140 508 666 380 2269 1384 268 Cuts at: 8116 1632 Size: 1917 Fragments arranged by size: 2269 1917 1384 869 666 508 380 268 140 AfeI AGC'GCT Cuts at: 4742 4742 Size: 8401 AflII C'TTAA_G Cuts at: 820 1017 4430 820 Size: 197 3413 4791 Fragments arranged by size: 4791 3413 197 AflIII A'CryG_T Cuts at: 1203 3167 4482 4719 7300 8277 1203 Size: 1964 1315 237 2581 977 1327 Fragments arranged by size: 2581 1964 1327 1315 977 237 AgeI A'CCGG_T Cuts at: 7232 7232 Size: 8401 AhdI GACnn_n'nnGTC Cuts at: 2071 7130 2071 Size: 5059 3342 AleI CACnn'nnGTG Cuts at: 1166 3465 3992 4220 1166 Size: 2299 527 228 5347 Fragments arranged by size: 5347 2299 527 228 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2264 5604 1184 Size: 1080 3340 3981 Fragments arranged by size: 3981 3340 1080 AlwNI CAG_nnn'CTG Cuts at: 3883 4978 5020 5077 6144 7716 3883 Size: 1095 42 57 1067 1572 4568 Fragments arranged by size: 4568 1572 1095 1067 57 42 ApaI G_GGCC'C Cuts at: 2104 4706 5301 2104 Size: 2602 595 5204 Fragments arranged by size: 5204 2602 595 ApaLI G'TGCA_C Cuts at: 2344 2512 4052 4142 5594 6458 7614 2344 Size: 168 1540 90 1452 864 1156 3131 Fragments arranged by size: 3131 1540 1452 1156 864 168 90 ApoI r'AATT_y Cuts at: 1493 5710 5936 6088 8197 1493 Size: 4217 226 152 2109 1697 Fragments arranged by size: 4217 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6955 160 Size: 872 5923 1606 Fragments arranged by size: 5923 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8401 AvaI C'yCGr_G Cuts at: 1870 1943 3299 5171 5210 5726 1870 Size: 73 1356 1872 39 516 4545 Fragments arranged by size: 4545 1872 1356 516 73 39 BamHI G'GATC_C Cuts at: 5731 6098 5731 Size: 367 8034 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4375 4444 4805 4958 5309 Size: 325 217 627 2588 69 361 153 351 Cuts at: 5309 5522 5722 7177 618 Size: 213 200 1455 1842 Fragments arranged by size: 2588 1842 1455 627 361 351 325 217 213 200 153 69 BanII G_rGCy'C Cuts at: 1391 2104 3656 4494 4706 4947 5178 5301 5720 Size: 713 1552 838 212 241 231 123 419 Cuts at: 5720 1391 Size: 4072 Fragments arranged by size: 4072 1552 838 713 419 241 231 212 123 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2489 2743 3137 3672 4018 5247 5545 Size: 971 590 254 394 535 346 1229 298 Cuts at: 5545 928 Size: 3784 Fragments arranged by size: 3784 1229 971 590 535 394 346 298 254 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6592 6626 1903 Size: 34 4655 34 3678 Fragments arranged by size: 4655 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3747 6293 7503 3747 Size: 2546 1210 4645 Fragments arranged by size: 4645 2546 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8401 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4164 7012 136 Size: 107 122 71 631 605 2492 2848 1525 Fragments arranged by size: 2848 2492 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3174 5613 8396 3174 Size: 2439 2783 3179 Fragments arranged by size: 3179 2783 2439 BlpI GC'TnA_GC Cuts at: 4401 4990 5818 4401 Size: 589 828 6984 Fragments arranged by size: 6984 828 589 Bme1580I G_kGCm'C Cuts at: 1790 2104 2243 2348 2516 2819 2828 3836 4056 Size: 314 139 105 168 303 9 1008 220 Cuts at: 4056 4146 4706 4808 5301 5331 5598 5636 6462 Size: 90 560 102 493 30 267 38 826 Cuts at: 6462 7618 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1008 826 560 493 314 303 267 220 168 139 105 102 90 38 30 9 BmgBI CAC'GTC Cuts at: 1318 3906 4147 4477 1318 Size: 2588 241 330 5242 Fragments arranged by size: 5242 2588 330 241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2933 3650 3832 Size: 416 53 83 399 24 1516 717 182 Cuts at: 3832 4120 6132 7090 442 Size: 288 2012 958 1753 Fragments arranged by size: 2012 1753 1516 958 717 416 399 288 182 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8401 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2854 3001 2854 Size: 147 8254 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2136 2149 2479 2696 2875 2947 3137 3827 Size: 897 13 330 217 179 72 190 690 Cuts at: 3827 4328 5267 7061 1239 Size: 501 939 1794 2579 Fragments arranged by size: 2579 1794 939 897 690 501 330 217 190 179 72 13 Bpu10I CC'TnA_GC Cuts at: 2829 2829 Size: 8401 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2311 2453 3743 4931 4961 5036 6526 7391 7689 Size: 142 1290 1188 30 75 1490 865 298 Cuts at: 7689 2311 Size: 3023 Fragments arranged by size: 3023 1490 1290 1188 865 298 142 75 30 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4199 5282 7064 882 Size: 617 2700 1083 1782 2219 Fragments arranged by size: 2700 2219 1782 1083 617 BsaAI yAC'GTr Cuts at: 493 1204 2686 5698 6192 493 Size: 711 1482 3012 494 2702 Fragments arranged by size: 3012 2702 1482 711 494 BsaBI GATnn'nnATC Cuts at: 5393 8395 5393 Size: 3002 5399 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4997 5091 6208 6590 Size: 53 83 186 936 3464 94 1117 382 Cuts at: 6590 7225 275 Size: 635 1451 Fragments arranged by size: 3464 1451 1117 936 635 382 186 94 83 53 BsaWI w'CCGG_w Cuts at: 3493 6834 7232 7506 7653 8064 3493 Size: 3341 398 274 147 411 3830 Fragments arranged by size: 3830 3341 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3392 3422 3707 3737 3842 Size: 30 165 30 1989 30 285 30 105 Cuts at: 3842 3872 4217 4247 4481 4511 1178 Size: 30 345 30 234 30 5068 Fragments arranged by size: 5068 1989 345 285 234 165 105 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 2392 2527 2684 2830 4856 4859 5471 Size: 1057 149 135 157 146 2026 3 612 Cuts at: 5471 1186 Size: 4116 Fragments arranged by size: 4116 2026 1057 612 157 149 146 135 3 BseYI C'CCAG_C Cuts at: 3153 7604 8100 3153 Size: 4451 496 3454 Fragments arranged by size: 4451 3454 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3215 3521 3529 3785 3991 4081 5236 Size: 573 1479 306 8 256 206 90 1155 Cuts at: 5236 1163 Size: 4328 Fragments arranged by size: 4328 1479 1155 573 306 256 206 90 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2389 4353 4580 5185 5851 6612 Size: 275 186 388 1964 227 605 666 761 Cuts at: 6612 7640 1540 Size: 1028 2301 Fragments arranged by size: 2301 1964 1028 761 666 605 388 275 227 186 BsiHKAI G_wGCw'C Cuts at: 2348 2516 2691 3219 3336 3410 3447 4056 4146 Size: 168 175 528 117 74 37 609 90 Cuts at: 4146 4686 4860 4947 5502 5598 6462 6547 7618 Size: 540 174 87 555 96 864 85 1071 Cuts at: 7618 2348 Size: 3131 Fragments arranged by size: 3131 1071 864 609 555 540 528 175 174 168 117 96 90 87 85 74 37 BsmI GAATG_Cn' Cuts at: 2733 5912 6005 2733 Size: 3179 93 5129 Fragments arranged by size: 5129 3179 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2163 3349 2163 Size: 1186 7215 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2243 2348 2516 2691 2819 2828 Size: 399 314 139 105 168 175 128 9 Cuts at: 2828 3219 3336 3410 3447 3656 3836 4056 4146 Size: 391 117 74 37 209 180 220 90 Cuts at: 4146 4494 4686 4706 4808 4860 4947 5178 5301 Size: 348 192 20 102 52 87 231 123 Cuts at: 5301 5331 5502 5598 5636 5720 6462 6547 7618 Size: 30 171 96 38 84 742 85 1071 Cuts at: 7618 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 391 348 314 231 220 209 192 180 175 171 168 139 128 123 117 105 102 96 90 87 85 84 74 52 38 37 30 20 9 BspEI T'CCGG_A Cuts at: 8064 8064 Size: 8401 BspHI T'CATG_A Cuts at: 6290 8020 6290 Size: 1730 6671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 2733 3969 3981 4129 4143 4326 4422 Size: 1210 679 1236 12 148 14 183 96 Cuts at: 4422 4695 4887 5251 5756 844 Size: 273 192 364 505 3489 Fragments arranged by size: 3489 1236 1210 679 505 364 273 192 183 148 96 14 12 BsrBI CCG'CTC Cuts at: 1394 1823 6288 1394 Size: 429 4465 3507 Fragments arranged by size: 4465 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4081 6896 7070 65 Size: 1183 2833 2815 174 1396 Fragments arranged by size: 2833 2815 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2388 2877 4554 5120 5184 5195 7045 Size: 110 578 489 1677 566 64 11 1850 Cuts at: 7045 7232 1700 Size: 187 2869 Fragments arranged by size: 2869 1850 1677 578 566 489 187 110 64 11 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8401 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8401 BssSI C'ACGA_G Cuts at: 2309 2432 5435 6461 7473 2309 Size: 123 3003 1026 1012 3237 Fragments arranged by size: 3237 3003 1026 1012 123 BstAPI GCAn_nnn'nTGC Cuts at: 794 2352 2520 2739 3294 3405 3750 4104 4423 Size: 1558 168 219 555 111 345 354 319 Cuts at: 4423 5358 794 Size: 935 3837 Fragments arranged by size: 3837 1558 935 555 354 345 319 219 168 111 BstBI TT'CG_AA Cuts at: 2007 7205 2007 Size: 5198 3203 BstEII G'GTnAC_C Cuts at: 1184 4777 1184 Size: 3593 4808 BstYI r'GATC_y Cuts at: 1972 2316 2472 3120 3174 4950 5388 5613 5731 Size: 344 156 648 54 1776 438 225 118 Cuts at: 5731 6098 6483 6500 7952 8038 8050 8396 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1776 1452 648 438 385 367 346 344 225 156 118 86 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2745 4440 2745 Size: 1695 6706 BtgI C'CryG_G Cuts at: 513 1065 2022 3411 4530 4954 513 Size: 552 957 1389 1119 424 3960 Fragments arranged by size: 3960 1389 1119 957 552 424 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5304 487 Size: 683 56 4078 3584 Fragments arranged by size: 4078 3584 683 56 BtsI GCAGTG_nn' Cuts at: 2425 2735 2737 2768 3283 3349 3460 3472 3481 Size: 310 2 31 515 66 111 12 9 Cuts at: 3481 4003 4238 4858 5224 5917 6711 6731 2425 Size: 522 235 620 366 693 794 20 4095 Fragments arranged by size: 4095 794 693 620 522 515 366 310 235 111 66 31 20 12 9 2 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8401 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8366 DraI TTT'AAA Cuts at: 5705 5705 Size: 8401 DraIII CAC_nnn'GTG Cuts at: 2520 3919 4014 4336 4777 2520 Size: 1399 95 322 441 6144 Fragments arranged by size: 6144 1399 441 322 95 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7408 809 Size: 507 6092 1802 Fragments arranged by size: 6092 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2595 3108 4173 Size: 54 1425 50 275 18 765 513 1065 Cuts at: 4173 4365 4386 4577 5011 5472 5848 6737 8366 Size: 192 21 191 434 461 376 889 1629 Cuts at: 8366 8 Size: 43 Fragments arranged by size: 1629 1425 1065 889 765 513 461 434 376 275 192 191 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 4577 5848 1537 Size: 275 2765 1271 4090 Fragments arranged by size: 4090 2765 1271 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2297 2369 6331 1353 Size: 944 72 3962 3423 Fragments arranged by size: 3962 3423 944 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2735 6988 7362 7508 387 Size: 1064 138 1146 4253 374 146 1280 Fragments arranged by size: 4253 1280 1146 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4945 4945 Size: 8401 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2149 2479 2501 2641 2696 2875 Size: 393 504 13 330 22 140 55 179 Cuts at: 2875 2947 3137 3149 3815 3827 4195 4328 5267 Size: 72 190 12 666 12 368 133 939 Cuts at: 5267 6464 7061 7848 8116 1239 Size: 1197 597 787 268 1524 Fragments arranged by size: 1524 1197 939 787 666 597 504 393 368 330 268 190 179 140 133 72 55 22 13 12 12 EcoNI CCTnn'n_nnAGG Cuts at: 2225 4016 2225 Size: 1791 6610 EcoO109I rG'GnC_Cy Cuts at: 4702 4703 5074 5298 4702 Size: 1 371 224 7805 Fragments arranged by size: 7805 371 224 1 EcoRI G'AATT_C Cuts at: 5710 6088 5710 Size: 378 8023 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2548 2644 3112 4210 5665 2548 Size: 96 468 1098 1455 5284 Fragments arranged by size: 5284 1455 1098 468 96 FspI TGC'GCA Cuts at: 4883 6907 4883 Size: 2024 6377 HaeII r_GCGC'y Cuts at: 1678 2952 2970 3714 4744 5142 7548 1678 Size: 1274 18 744 1030 398 2406 2531 Fragments arranged by size: 2531 2406 1274 1030 744 398 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 2057 2089 2165 Size: 32 135 32 446 32 7 32 76 Cuts at: 2165 2197 3842 3874 4580 4612 7042 7074 7116 Size: 32 1645 32 706 32 2430 32 42 Cuts at: 7116 7148 1373 Size: 32 2626 Fragments arranged by size: 2626 2430 1645 706 446 135 76 42 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2758 3909 5745 5991 669 Size: 988 281 820 1151 1836 246 3079 Fragments arranged by size: 3079 1836 1151 988 820 281 246 HindIII A'AGCT_T Cuts at: 748 5084 748 Size: 4336 4065 HpaI GTT'AAC Cuts at: 5991 5991 Size: 8401 KpnI G_GTAC'C Cuts at: 4379 5726 4379 Size: 1347 7054 MfeI C'AATT_G Cuts at: 6000 6000 Size: 8401 MluI A'CGCG_T Cuts at: 4482 8277 4482 Size: 3795 4606 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2583 3792 4467 7515 7699 1305 Size: 1278 1209 675 3048 184 2007 Fragments arranged by size: 3048 2007 1278 1209 675 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2597 4388 5013 10 Size: 54 1425 343 765 1791 625 3398 Fragments arranged by size: 3398 1791 1425 765 625 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 3465 3992 4049 4074 4220 4535 Size: 648 1026 1273 527 57 25 146 315 Cuts at: 4535 5261 5347 6359 6718 6877 518 Size: 726 86 1012 359 159 2042 Fragments arranged by size: 2042 1273 1026 1012 726 648 527 359 315 159 146 86 57 25 MspA1I CmG'CkG Cuts at: 1606 2109 2707 3013 3697 4095 4905 4994 5817 Size: 503 598 306 684 398 810 89 823 Cuts at: 5817 6494 7642 7887 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 823 810 684 677 598 503 398 306 245 89 NaeI GCC'GGC Cuts at: 5122 5197 5122 Size: 75 8326 NcoI C'CATG_G Cuts at: 513 1065 2022 3411 4530 4954 513 Size: 552 957 1389 1119 424 3960 Fragments arranged by size: 3960 1389 1119 957 552 424 NdeI CA'TA_TG Cuts at: 387 387 Size: 8401 NgoMIV G'CCGG_C Cuts at: 5120 5195 5120 Size: 75 8326 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8401 NotI GC'GGCC_GC Cuts at: 5848 5848 Size: 8401 NspI r_CATG'y Cuts at: 1132 1229 2566 3171 3985 4426 4723 4921 4972 Size: 97 1337 605 814 441 297 198 51 Cuts at: 4972 5260 5759 7304 1132 Size: 288 499 1545 2229 Fragments arranged by size: 2229 1545 1337 814 605 499 441 297 288 198 97 51 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8401 PciI A'CATG_T Cuts at: 3167 4719 7300 3167 Size: 1552 2581 4268 Fragments arranged by size: 4268 2581 1552 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8401 PfoI T'CCnGG_A Cuts at: 2113 2113 Size: 8401 PmeI GTTT'AAAC Cuts at: 5705 5705 Size: 8401 PmlI CAC'GTG Cuts at: 2686 2686 Size: 8401 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6470 6502 7983 8015 6470 Size: 32 1481 32 6856 Fragments arranged by size: 6856 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5074 5074 Size: 8401 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8401 PsiI TTA'TAA Cuts at: 5971 5971 Size: 8401 PspOMI G'GGCC_C Cuts at: 2100 4702 5297 2100 Size: 2602 595 5204 Fragments arranged by size: 5204 2602 595 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3230 3262 3230 Size: 32 8369 PstI C_TGCA'G Cuts at: 830 2357 2556 2730 3330 3467 3879 3966 4140 Size: 1527 199 174 600 137 412 87 174 Cuts at: 4140 4233 4280 4419 4548 4692 4836 5025 5667 Size: 93 47 139 129 144 144 189 642 Cuts at: 5667 5753 830 Size: 86 3478 Fragments arranged by size: 3478 1527 642 600 412 199 189 174 174 144 144 139 137 129 93 87 86 47 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8401 PvuII CAG'CTG Cuts at: 2707 2707 Size: 8401 SacI G_AGCT'C Cuts at: 4947 4947 Size: 8401 SalI G'TCGA_C Cuts at: 1936 2756 3907 5743 1936 Size: 820 1151 1836 4594 Fragments arranged by size: 4594 1836 1151 820 SbfI CC_TGCA'GG Cuts at: 3330 4140 4419 5753 3330 Size: 810 279 1334 5978 Fragments arranged by size: 5978 1334 810 279 ScaI AGT'ACT Cuts at: 6649 8361 6649 Size: 1712 6689 SexAI A'CCwGG_T Cuts at: 5076 5076 Size: 8401 SfcI C'TryA_G Cuts at: 826 1045 2353 2552 2726 3067 3326 3463 3875 Size: 219 1308 199 174 341 259 137 412 Cuts at: 3875 3962 4136 4229 4276 4415 4544 4688 4832 Size: 87 174 93 47 139 129 144 144 Cuts at: 4832 5021 5663 5749 6884 7565 7756 826 Size: 189 642 86 1135 681 191 1471 Fragments arranged by size: 1471 1308 1135 681 642 412 341 259 219 199 191 189 174 174 144 144 139 137 129 93 87 86 47 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8401 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8401 SmaI CCC'GGG Cuts at: 1872 5728 1872 Size: 3856 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2290 2468 3758 4430 4940 4946 Size: 197 926 347 178 1290 672 510 6 Cuts at: 4946 5051 6505 7406 7668 820 Size: 105 1454 901 262 1553 Fragments arranged by size: 1553 1454 1290 926 901 672 510 347 262 197 178 105 6 SnaBI TAC'GTA Cuts at: 493 5698 6192 493 Size: 5205 494 2702 Fragments arranged by size: 5205 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8401 SphI G_CATG'C Cuts at: 1132 4426 4921 5759 1132 Size: 3294 495 838 3774 Fragments arranged by size: 3774 3294 838 495 SspI AAT'ATT Cuts at: 5 52 6325 5 Size: 47 6273 2081 Fragments arranged by size: 6273 2081 47 StuI AGG'CCT Cuts at: 1517 4286 1517 Size: 2769 5632 StyI C'CwwG_G Cuts at: 513 1065 2022 3057 3411 3639 4035 4176 4530 Size: 552 957 1035 354 228 396 141 354 Cuts at: 4530 4954 5840 513 Size: 424 886 3074 Fragments arranged by size: 3074 1035 957 886 552 424 396 354 354 228 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2137 2200 4367 5144 5327 5672 6428 Size: 1255 557 63 2167 777 183 345 756 Cuts at: 6428 6445 6598 6783 7228 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2167 1498 1255 777 756 557 445 345 185 183 153 63 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2920 6647 8359 Size: 274 80 33 51 1443 943 3727 1712 Cuts at: 8359 96 Size: 138 Fragments arranged by size: 3727 1712 1443 943 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2714 2746 4217 4249 8361 8393 2714 Size: 32 1471 32 4112 32 2722 Fragments arranged by size: 4112 2722 1471 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3904 1316 Size: 2588 5813 XbaI T'CTAG_A Cuts at: 5737 5737 Size: 8401 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2111 2142 2971 4303 5149 2111 Size: 31 829 1332 846 5363 Fragments arranged by size: 5363 1332 846 829 31 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8401 XmaI C'CCGG_G Cuts at: 1870 5726 1870 Size: 3856 4545 XmnI GAAnn'nnTTC Cuts at: 5645 6530 5645 Size: 885 7516 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5092 6209 276 Size: 53 83 186 936 3558 1117 2468 Fragments arranged by size: 3558 2468 1117 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI